BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,583	A→G	Y403H (TAT→CAT)	araJ ←	AraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,583	0	A	G	99.7%	1136.4 / ‑5.4	355	Y403H (TAT→CAT)	araJ	AraJ
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (165/189); total (165/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 371 total aligned reads displayed.

AATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATAC  >  NC_000913/410493‑410672

aaTAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGca                                                                                         <  2:6839615/93‑1 (MQ=255)

aaTAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGca                                                                                         >  2:5620204/1‑93 (MQ=255)

aaTAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGca                                                                                         <  1:4932801/93‑1 (MQ=255)

aaTAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGca                                                                                         <  2:6734584/93‑1 (MQ=255)

  tAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGcagc                                                                                       <  1:7229591/93‑1 (MQ=255)

   aaGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATgcagca                                                                                      >  1:4161498/1‑93 (MQ=255)

    aGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGcagcag                                                                                     <  1:167067/93‑1 (MQ=255)

       aaTGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCaa                                                                                  <  2:1225420/93‑1 (MQ=255)

       aaTGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCaa                                                                                  <  1:3657457/93‑1 (MQ=255)

        aTGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAac                                                                                 <  2:5439903/93‑1 (MQ=255)

        aTGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAac                                                                                 <  2:6916511/93‑1 (MQ=255)

          gAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAacga                                                                               >  2:3389703/1‑93 (MQ=255)

          gAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAacga                                                                               <  1:6241462/93‑1 (MQ=255)

           aGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAacgac                                                                              <  2:1489830/93‑1 (MQ=255)

           aGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAacgac                                                                              <  1:5112835/93‑1 (MQ=255)

              aCCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACgacgac                                                                           <  2:7281929/93‑1 (MQ=255)

              aCCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACgacgac                                                                           <  1:6775938/93‑1 (MQ=255)

              aCCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACgacgac                                                                           >  2:3728946/1‑93 (MQ=255)

              aCCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACgacgac                                                                           <  2:442437/93‑1 (MQ=255)

               ccGAGACTATAACCTCCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACa                                                                          <  1:5638793/93‑1 (MQ=255)

                cGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACAt                                                                         >  1:7932840/1‑93 (MQ=255)

                 gagaCTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATc                                                                        <  2:4271992/93‑1 (MQ=255)

                 gagaCTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATc                                                                        <  1:4502233/93‑1 (MQ=255)

                   gaCTATAACCTCCCCCAGTGGTTTCGCCAGCACCGGAGTCTCCGCCGCTTGATGGCGCTTATAGCGACCATGCAGCAGCAACGACGGCATCgc                                                                      <  1:7003068/93‑1 (MQ=255)

                   gaCTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgc                                                                      >  2:6380459/1‑93 (MQ=255)

                   gaCTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgc                                                                      >  1:2049477/1‑93 (MQ=255)

                   gaCTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgc                                                                      >  1:3559018/1‑93 (MQ=255)

                   gaCTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgc                                                                      >  2:2480633/1‑93 (MQ=255)

                      tataACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgcagc                                                                   >  2:1769775/1‑93 (MQ=255)

                      tataACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgcagc                                                                   >  2:5906118/1‑93 (MQ=255)

                      tataACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgcagc                                                                   >  2:6761081/1‑93 (MQ=255)

                      tataACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCgcaga                                                                   >  2:1683803/1‑92 (MQ=255)

                          aCCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGCCATCGCAGCAAAc                                                               >  2:2164490/1‑93 (MQ=255)

                             tACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGaa                                                            >  2:2960456/1‑93 (MQ=255)

                             tACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGaa                                                            <  2:304129/93‑1 (MQ=255)

                             caCCCCAGTGGTTTCGCCAGCACCGGAGTAGCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACAGCGCAGCAAACGaa                                                            <  2:6526359/92‑1 (MQ=255)

                              cccccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGaaa                                                           <  2:4791477/92‑1 (MQ=255)

                              aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGaaa                                                           <  1:4248738/93‑1 (MQ=255)

                              aCCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGaaa                                                           <  1:1053942/93‑1 (MQ=255)

                               ccccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAg                                                          >  1:7743981/1‑93 (MQ=255)

                               ccccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAg                                                          <  1:7353705/93‑1 (MQ=255)

                               ccccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAg                                                          >  1:6272391/1‑93 (MQ=255)

                               ccccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAg                                                          <  1:6099745/93‑1 (MQ=255)

                               ccccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTAGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAg                                                          <  2:3958415/93‑1 (MQ=255)

                                cccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGc                                                         >  1:2411492/1‑93 (MQ=255)

                                 ccAGTGGTTTCGCTAGCACCGTAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        <  1:4845054/93‑1 (MQ=255)

                                 ccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        >  1:603543/1‑93 (MQ=255)

                                 ccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        <  2:291739/93‑1 (MQ=255)

                                 ccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        >  2:5020112/1‑93 (MQ=255)

                                 ccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        <  1:5378962/93‑1 (MQ=255)

                                 ccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        <  2:1397352/93‑1 (MQ=255)

                                 ccAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                                                        <  2:6005155/93‑1 (MQ=255)

                                  cAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAg                                                       <  1:1577509/93‑1 (MQ=255)

                                  cAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAg                                                       <  1:3802756/93‑1 (MQ=255)

                                   aGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAgg                                                      <  2:3184718/93‑1 (MQ=255)

                                     tGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGggc                                                    <  2:5110416/93‑1 (MQ=255)

                                       gTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGggcgg                                                  >  1:1261778/1‑93 (MQ=255)

                                       gTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGggcgg                                                  <  1:5838680/93‑1 (MQ=255)

                                         ttCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGCCCATGCAGCAGCACCGCCGACATCGCAGGAAACGACAGCCGGGCggca                                                >  1:3437117/1‑93 (MQ=255)

                                         ttCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCggca                                                <  2:1312731/93‑1 (MQ=255)

                                          tCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCggcag                                               >  1:4704852/1‑93 (MQ=255)

                                            gCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCggcaggc                                             >  2:6225354/1‑93 (MQ=255)

                                             ccAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCggcaggca                                            <  1:3064441/93‑1 (MQ=255)

                                              cAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGgcaggcag                                           <  1:5725518/93‑1 (MQ=255)

                                                gCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                                         <  1:6629947/93‑1 (MQ=255)

                                                gCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                                         >  1:6200792/1‑93 (MQ=255)

                                                gCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                                         >  1:2531517/1‑93 (MQ=255)

                                                    cGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCAc                                     >  1:3951837/1‑93 (MQ=255)

                                                     ggAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACg                                    >  1:1931038/1‑93 (MQ=255)

                                                          aTCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAAtt                               <  2:4923779/93‑1 (MQ=255)

                                                          aTCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAAtt                               <  2:7710745/93‑1 (MQ=255)

                                                          aTCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAAtt                               >  1:2257269/1‑93 (MQ=255)

                                                           tCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGATGACATCGCAGCAAACGAAAGCAGTGCGGCAGGCAGCGCCACGTAATta                              >  2:6993344/1‑93 (MQ=255)

                                                           tCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCCGGCAGCGCCACGTAATta                              >  2:2292447/1‑93 (MQ=255)

                                                           tCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATta                              <  2:806399/93‑1 (MQ=255)

                                                           tCCGCCGCTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATta                              <  1:1132997/93‑1 (MQ=255)

                                                             cgccgcTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGTGGCAGGCAGCGCCACGTAATtata                            <  2:7587285/93‑1 (MQ=255)

                                                             cgccgcTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtata                            <  1:687447/93‑1 (MQ=255)

                                                             cgccgcTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtata                            <  1:4422216/93‑1 (MQ=255)

                                                               ccgcTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATg                          >  2:255024/1‑93 (MQ=255)

                                                                 gcTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGcc                        >  1:6549126/1‑93 (MQ=255)

                                                                  cTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCa                       <  2:5804903/93‑1 (MQ=255)

                                                                  cTTGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCa                       <  1:5447796/93‑1 (MQ=255)

                                                                   ttGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAg                      >  1:960355/1‑93 (MQ=255)

                                                                   ttGCTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAg                      >  1:3122639/1‑93 (MQ=255)

                                                                      cTGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGccc                   >  1:2934104/1‑93 (MQ=255)

                                                                       tGGCGCTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGCAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGcccc                  >  2:7130981/1‑93 (MQ=255)

                                                                          cgcTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGc               <  1:6394191/93‑1 (MQ=255)

                                                                          cgcTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGc               >  1:2176374/1‑93 (MQ=255)

                                                                            cTTATAGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGT‑ATTATATGCCAGCCCCAGCGTc            <  2:3823787/93‑1 (MQ=255)

                                                                              tataGCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCa           >  2:4055329/1‑93 (MQ=255)

                                                                                  gCGACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcat       >  1:7541293/1‑93 (MQ=255)

                                                                                    gACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTCATTATATGCCAGCCCCAGCGTCAGaatca     >  1:2690863/1‑93 (MQ=255)

                                                                                    gACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatca     <  1:6365123/93‑1 (MQ=255)

                                                                                    gACCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatca     <  2:1883627/93‑1 (MQ=255)

                                                                                     aCCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatcat    >  1:4599322/1‑93 (MQ=255)

                                                                                     aCCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatcat    >  2:2766149/1‑93 (MQ=255)

                                                                                     aCCATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGcatcat    >  2:4586863/1‑93 (MQ=255)

                                                                                       cATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATAc  <  2:102853/93‑1 (MQ=255)

                                                                                       cATGCAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATAc  <  2:7457753/93‑1 (MQ=255)

AATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGCCAGCCCCAGCGTCAGCATCATAC  >  NC_000913/410493‑410672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`