BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,913	A→G	R75R (CGT→CGC)	yhjW ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,913	0	A	G	99.8%	1329.7 / ‑5.3	423	R75R (CGT→CGC)	yhjW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (202/219); total (203/220)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 452 total aligned reads displayed.

GAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGACGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCT  >  NC_000913/3707823‑3708002

  aggTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgta                                                                                       >  1:5142678/1‑93 (MQ=255)

  aggTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgta                                                                                       <  2:7929102/93‑1 (MQ=255)

  aggTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgta                                                                                       <  2:7829996/93‑1 (MQ=255)

  aggTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgta                                                                                       <  1:6968537/93‑1 (MQ=255)

  aggTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgta                                                                                       >  2:7460772/1‑93 (MQ=255)

   ggTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgtag                                                                                      >  2:3236347/1‑93 (MQ=255)

    gTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgtagt                                                                                     <  1:1203829/93‑1 (MQ=255)

    gTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCgtagt                                                                                     >  2:25934/1‑93 (MQ=255)

      cATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTaa                                                                                   <  2:3514437/93‑1 (MQ=255)

        tGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTaaaa                                                                                 >  1:4796648/1‑93 (MQ=255)

         gTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTaaaaa                                                                                <  1:1504492/93‑1 (MQ=255)

         gTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTaaaaa                                                                                >  1:6662020/1‑93 (MQ=255)

            aTAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAaagaa                                                                             >  2:1876280/1‑93 (MQ=255)

             tAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAaagaag                                                                            >  1:3009211/1‑93 (MQ=255)

               gCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGt                                                                          >  2:6930234/1‑93 (MQ=255)

               acTGGCACCTGCGGAAAAGAGCTCCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGt                                                                          <  1:6214136/92‑1 (MQ=255)

                  ggCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCAc                                                                       >  1:5808319/1‑93 (MQ=255)

                   gCACCTGCGGAAAAGAGCACCACAAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCAcc                                                                      <  2:4309007/93‑1 (MQ=255)

                    cACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCa                                                                     <  2:6973049/93‑1 (MQ=255)

                    cACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCa                                                                     >  1:5617247/1‑93 (MQ=255)

                    cACCGGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCa                                                                     <  2:6630706/93‑1 (MQ=255)

                     cccTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAg                                                                    <  1:7473959/92‑1 (MQ=255)

                     aCCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGGCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAg                                                                    >  2:888952/1‑93 (MQ=255)

                       cTGCGGAAAAGAGCCCCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTa                                                                  <  2:5750865/93‑1 (MQ=255)

                       cTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTa                                                                  <  2:5324044/93‑1 (MQ=255)

                       cTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACAAGTa                                                                  <  2:1724020/93‑1 (MQ=255)

                        tGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTAc                                                                 >  2:506569/1‑93 (MQ=255)

                        tGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTAc                                                                 <  1:1428125/93‑1 (MQ=255)

                         gCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACg                                                                <  1:2477270/93‑1 (MQ=255)

                         gCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACg                                                                >  1:3184507/1‑93 (MQ=255)

                         gCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACg                                                                <  2:6894231/93‑1 (MQ=255)

                          cGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACgg                                                               <  2:4558979/93‑1 (MQ=255)

                            gAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTg                                                             <  2:6227589/93‑1 (MQ=255)

                               aaGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTGAAAAGAAGGTCACCAGTACGGTggcg                                                          >  2:4121566/1‑93 (MQ=255)

                               aaGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGGAGCAAAAAGAAGGTCACCAGTACGGTggcg                                                          >  2:2009922/1‑93 (MQ=255)

                                agagCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTggcgg                                                         >  2:6713890/1‑93 (MQ=255)

                                 gagCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTggcggc                                                        >  2:47984/1‑93 (MQ=255)

                                  agCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGcc                                                       >  1:5051700/1‑93 (MQ=255)

                                  agCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGcc                                                       <  1:2329560/93‑1 (MQ=255)

                                   gCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCa                                                      >  1:5006103/1‑93 (MQ=255)

                                   gCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCa                                                      <  2:1295712/93‑1 (MQ=255)

                                       caccaGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTc                                                  <  1:5478474/93‑1 (MQ=255)

                                          caGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTcaac                                               <  1:3508899/93‑1 (MQ=255)

                                           aGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTcaaca                                              <  2:5431270/93‑1 (MQ=255)

                                            gCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTcaacaa                                             >  1:5891250/1‑93 (MQ=255)

                                            gCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTcaacaa                                             >  1:1820257/1‑93 (MQ=255)

                                            gCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTcaacaa                                             <  2:1464351/93‑1 (MQ=255)

                                             cGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCaacaac                                            <  2:5986946/93‑1 (MQ=255)

                                             cGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCaacaac                                            <  1:1093867/93‑1 (MQ=255)

                                             cGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCaacaac                                            <  1:4194947/93‑1 (MQ=255)

                                              gATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAacaaca                                           <  1:1657493/93‑1 (MQ=255)

                                              gATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAacaaca                                           <  1:4387088/93‑1 (MQ=255)

                                              gATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAacaaca                                           <  2:2362554/93‑1 (MQ=255)

                                                 gCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAAcagca                                        <  1:4088064/93‑1 (MQ=255)

                                                    aGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGaa                                     <  1:7169525/93‑1 (MQ=255)

                                                    aGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGaa                                     <  1:2592519/93‑1 (MQ=255)

                                                     gAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGaaa                                    <  2:3657851/93‑1 (MQ=255)

                                                     gAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGaaa                                    <  1:4308688/93‑1 (MQ=255)

                                                      aaTACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAAt                                   <  1:5574450/93‑1 (MQ=255)

                                                           gCCAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCtt                              >  1:4418774/1‑93 (MQ=255)

                                                             cAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTc                            <  2:7540202/93‑1 (MQ=255)

                                                             cAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTc                            <  2:7297408/93‑1 (MQ=255)

                                                             cAGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTc                            <  1:5965147/93‑1 (MQ=255)

                                                              aGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTcc                           >  1:5296965/1‑93 (MQ=255)

                                                              aGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTcc                           <  1:5668181/93‑1 (MQ=255)

                                                              aGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTcc                           <  1:288967/93‑1 (MQ=255)

                                                              aGCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTcc                           <  1:7353246/93‑1 (MQ=255)

                                                               gCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCa                          >  2:4908488/1‑93 (MQ=255)

                                                               gCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCa                          <  1:7561072/93‑1 (MQ=255)

                                                               gCTGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCa                          >  2:1133968/1‑93 (MQ=255)

                                                                 tGCGGCGGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGa                        >  1:6066727/1‑93 (MQ=255)

                                                                  gcggcgGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGAc                       <  2:2729327/93‑1 (MQ=255)

                                                                  gcggcgGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGAc                       <  2:2107312/93‑1 (MQ=255)

                                                                  gcggcgGCCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGAc                       <  1:3642085/93‑1 (MQ=255)

                                                                     gcggcCAAGCAGCGAATAAAGGCGTAGTAAAAAGAAGTTAACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGt                    <  2:2211517/93‑1 (MQ=255)

                                                                     gcggcCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACgga                    >  2:6538391/1‑92 (MQ=255)

                                                                       ggcCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTaa                  <  2:5469484/93‑1 (MQ=255)

                                                                       ggcCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTaa                  >  2:2816167/1‑93 (MQ=255)

                                                                       ggcCAAACAGCGAAAGAAGACGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTaa                  >  1:2183488/1‑93 (MQ=255)

                                                                        gcCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTaaa                 >  1:3430256/1‑93 (MQ=255)

                                                                        gcCAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTaaa                 >  2:3020320/1‑93 (MQ=255)

                                                                         ccAAACAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTaaaa                <  2:102079/93‑1 (MQ=255)

                                                                           aaaCAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATc              <  1:7823902/93‑1 (MQ=255)

                                                                           aaaCAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATc              >  1:4673585/1‑93 (MQ=255)

                                                                           aaaCAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATc              <  2:4190772/93‑1 (MQ=255)

                                                                             aCAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGt            <  2:3896256/93‑1 (MQ=255)

                                                                              cAGCGAAAGAAGGCGTAGTAAAAAGAAGGTCCCCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTg           <  1:4886745/93‑1 (MQ=255)

                                                                               aGCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTgc          <  1:2381547/93‑1 (MQ=255)

                                                                                gCGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTgcg         <  2:4011187/93‑1 (MQ=255)

                                                                                 cGAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTgcgc        >  1:866140/1‑93 (MQ=255)

                                                                                  gAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCa       >  2:634274/1‑93 (MQ=255)

                                                                                  gAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCa       >  1:6515502/1‑93 (MQ=255)

                                                                                  gAAAGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCa       >  1:1029924/1‑93 (MQ=255)

                                                                                   aaaGAAGGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCAt      <  1:3889714/93‑1 (MQ=255)

                                                                                    aagaagGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATa     >  2:4472353/1‑93 (MQ=255)

                                                                                    aagaagGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATa     <  1:6288660/93‑1 (MQ=255)

                                                                                    aagaagGCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATa     >  1:6856822/1‑93 (MQ=255)

                                                                                      gaaggCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGc   >  1:7686595/1‑93 (MQ=255)

                                                                                       aaggCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCt  <  2:6442590/93‑1 (MQ=255)

                                                                                       aaggCGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCt  <  2:2479825/93‑1 (MQ=255)

GAAGGTCATGTAATAGCTGGCACCTGCGGAAAAGAGCACCACCAGCGATGCCAGAATACGCCAGCTGCGGCGGCCAAACAGCGAAAGAAGACGTAGTAAAAAGAAGGTCACCAGTACGGTGGCGGCCAGTTCAACAACAGCAGAAATGCCTTTCCAGACGGTAAAATCGTGCGCATAGCT  >  NC_000913/3707823‑3708002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`