BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,633	T→C	L456L (CTA→CTG)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,633	0	T	C	99.6%	806.8 / ‑5.5	254	L456L (CTA→CTG)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (197/55); total (198/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 264 total aligned reads displayed.

GTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCC  >  NC_000913/3725544‑3725722

gTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGa                                                                                        >  1:6569026/1‑93 (MQ=255)

 tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAt                                                                                       >  2:7008636/1‑93 (MQ=255)

 tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAt                                                                                       >  2:6255482/1‑93 (MQ=255)

 tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAt                                                                                       >  1:6772076/1‑93 (MQ=255)

  ttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGCTg                                                                                      >  2:2676625/1‑93 (MQ=255)

  ttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATg                                                                                      >  2:2353091/1‑93 (MQ=255)

     cGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGa                                                                                   >  1:4714139/1‑93 (MQ=255)

     cGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGa                                                                                   >  2:3131922/1‑93 (MQ=255)

      gCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGAc                                                                                  >  2:4107332/1‑93 (MQ=255)

         aTTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGt                                                                               >  1:7038278/1‑93 (MQ=255)

         aTTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGt                                                                               >  1:3006526/1‑93 (MQ=255)

          ttAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGtt                                                                              >  2:5510936/1‑93 (MQ=255)

          ttAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGtt                                                                              >  1:4143767/1‑93 (MQ=255)

           tAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTc                                                                             >  2:7376060/1‑93 (MQ=255)

             aTGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTa                                                                           >  1:2388920/1‑93 (MQ=255)

              tGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTaa                                                                          >  1:7300981/1‑93 (MQ=255)

              tGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTaa                                                                          >  1:3209809/1‑93 (MQ=255)

                 cAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACgg                                                                       >  1:3857568/1‑93 (MQ=255)

                  aGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGt                                                                      >  1:1749985/1‑93 (MQ=255)

                    aaGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAg                                                                    <  2:2535385/93‑1 (MQ=255)

                     aGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGt                                                                   >  2:1094060/1‑93 (MQ=255)

                     aGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGt                                                                   <  1:395709/93‑1 (MQ=255)

                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  1:1616303/1‑93 (MQ=255)

                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  2:530647/1‑93 (MQ=255)

                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  1:6130001/1‑93 (MQ=255)

                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  2:4974010/1‑93 (MQ=255)

                        tGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGc                                                                <  1:7174126/93‑1 (MQ=255)

                         gCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCg                                                               >  1:354213/1‑93 (MQ=255)

                          cTGATAGAGGCGACGGAACGTTTCTCGTCGGGGCTGATAAGCGGCATAACGCTGCGCAACTGGCAGATGCGACTGTTCTAACTGTAGTTGCgg                                                              >  1:7631449/1‑93 (MQ=255)

                            gATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGca                                                            >  1:5356666/1‑93 (MQ=255)

                             aTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaa                                                           >  2:6711245/1‑93 (MQ=255)

                             aTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaa                                                           <  1:6403371/93‑1 (MQ=255)

                             aTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGAGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaa                                                           >  2:3763312/1‑93 (MQ=255)

                              tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGGGGcaac                                                          >  2:3620081/1‑93 (MQ=255)

                              tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaac                                                          >  2:5730521/1‑93 (MQ=255)

                              tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaac                                                          >  1:1577449/1‑93 (MQ=255)

                               agagGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaaca                                                         >  1:5618761/1‑93 (MQ=255)

                                gagGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaacaa                                                        >  2:7789120/1‑93 (MQ=255)

                                gagGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaacaa                                                        >  1:7023831/1‑93 (MQ=255)

                                  ggCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAtt                                                      >  1:1355572/1‑93 (MQ=255)

                                      gcGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAt                                                  <  1:6765509/92‑1 (MQ=255)

                                      aCGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAt                                                  >  2:3549031/1‑93 (MQ=255)

                                       cGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATg                                                 >  2:7847128/1‑93 (MQ=255)

                                        ggAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGa                                                >  1:4986377/1‑93 (MQ=255)

                                        ggAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGa                                                >  1:3355663/1‑93 (MQ=255)

                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  1:5539559/1‑93 (MQ=255)

                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               <  2:1801612/93‑1 (MQ=255)

                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  2:4711475/1‑93 (MQ=255)

                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  2:2303338/1‑93 (MQ=255)

                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  1:5722230/1‑93 (MQ=255)

                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGAATAACGCTGCGCATCTGGCAGAGGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  1:1127029/1‑93 (MQ=255)

                                          aaCGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGc                                              >  2:2814282/1‑93 (MQ=255)

                                            cGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGa                                            >  1:5375809/1‑93 (MQ=255)

                                             gTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAt                                           >  1:5046187/1‑93 (MQ=255)

                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTTAATGAGCGAtt                                          >  2:5594461/1‑93 (MQ=255)

                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          >  2:3852535/1‑93 (MQ=255)

                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          >  2:7488301/1‑93 (MQ=255)

                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          <  1:6050160/93‑1 (MQ=255)

                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          >  2:2979144/1‑93 (MQ=255)

                                                  tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTtctc                                      >  2:1589306/1‑93 (MQ=255)

                                                  tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTtctc                                      >  1:243312/1‑93 (MQ=255)

                                                   cgtcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTctct                                     >  2:6847076/1‑93 (MQ=255)

                                                    gtcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTg                                    <  2:606507/93‑1 (MQ=255)

                                                     tcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTgg                                   >  2:275138/1‑93 (MQ=255)

                                                      cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGa                                  <  1:3102139/93‑1 (MQ=255)

                                                      cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGa                                  >  2:1983061/1‑93 (MQ=255)

                                                       gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAt                                 >  1:4625615/1‑93 (MQ=255)

                                                       gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAt                                 >  1:6642629/1‑93 (MQ=255)

                                                       gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATCAGCGAATTTTCTGGAt                                 >  1:1014107/1‑93 (MQ=255)

                                                        tGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAtt                                >  2:2803859/1‑93 (MQ=255)

                                                         ggCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTc                               <  1:4487602/93‑1 (MQ=255)

                                                         ggCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTc                               <  2:5881821/93‑1 (MQ=255)

                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTcg                              >  2:5906447/1‑93 (MQ=255)

                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTcg                              >  2:4257243/1‑93 (MQ=255)

                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTcg                              <  2:4462077/93‑1 (MQ=255)

                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTCACGGCAACCATTCAATAAGCGATTTCTCTGGAttac                              >  1:2641984/1‑91 (MQ=255)

                                                           cTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGGGATTTCTCTGGATTcgc                             >  2:168488/1‑93 (MQ=255)

                                                            tGATAAGCGGCATAACGATGCGCATCTGGTAGATGCGACTGCTCTAACGGGAGTTAAGGAAACAATTGAATGAGCGCTTTCTCAGGAGTcgca                            >  2:5096809/1‑92 (MQ=255)

                                                             gATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTtgccg                           >  1:5712562/1‑93 (MQ=255)

                                                               tAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCcgcg                         >  1:1975174/1‑93 (MQ=255)

                                                                aaGCGGCATAACGCTGCGCATCTGGCCGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGACCGATTTCTCTGGATTCGACGCGa                        >  1:2487777/1‑93 (MQ=255)

                                                                aaGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGa                        >  1:2703981/1‑93 (MQ=255)

                                                                 aGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGAt                       <  2:7690082/93‑1 (MQ=255)

                                                                  gCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATc                      <  2:1738913/93‑1 (MQ=255)

                                                                  gCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATc                      <  1:5973721/93‑1 (MQ=255)

                                                                    ggCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTg                    <  2:5347657/93‑1 (MQ=255)

                                                                    ggCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTg                    >  2:4166879/1‑93 (MQ=255)

                                                                       aTAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTgcgc                 >  2:6432999/1‑93 (MQ=255)

                                                                        tAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGcc                >  1:873430/1‑93 (MQ=255)

                                                                         aaCGCTGCGCATCTGGCAGATACGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCa               >  1:6457699/1‑93 (MQ=255)

                                                                          aCGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAg              >  1:4271184/1‑93 (MQ=255)

                                                                            gCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGcc            <  1:3131923/93‑1 (MQ=255)

                                                                              tGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCtt          <  1:7448872/93‑1 (MQ=255)

                                                                              tGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCtt          <  1:2849980/93‑1 (MQ=255)

                                                                              tGCGCATCTGGCAGATGCGACTGTTCTAACGGGAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCACAGCGATCTGCGCCAGCCtt          >  2:2185567/1‑93 (MQ=255)

                                                                               gcgcATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtg         <  2:4896486/93‑1 (MQ=255)

                                                                                cgcATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtgc        <  2:923206/93‑1 (MQ=255)

                                                                                 gcATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtgct       <  1:2212126/93‑1 (MQ=255)

                                                                                   aTCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtgctgc     <  2:4610502/93‑1 (MQ=255)

                                                                                      tGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGcc  >  2:1299977/1‑93 (MQ=255)

GTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCC  >  NC_000913/3725544‑3725722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`