BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,233	T→C	L220P (CTG→CCG)	rfaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,233	0	T	C	99.3%	866.9 / ‑3.4	276	L220P (CTG→CCG)	rfaL	O‑antigen ligase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (171/103); total (171/105)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

Only 100 of 276 total aligned reads displayed.

CTAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTA  >  NC_000913/3795143‑3795322

cTAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGt                                                                                         <  2:7725610/93‑1 (MQ=255)

  aaGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTc                                                                                       <  2:5626564/93‑1 (MQ=255)

   aGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGCTcc                                                                                      >  1:4693446/1‑93 (MQ=255)

    gAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTccc                                                                                     <  2:7295728/93‑1 (MQ=255)

    gAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTccc                                                                                     <  2:4834945/93‑1 (MQ=255)

     aaaaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCt                                                                                    >  1:7474089/1‑93 (MQ=255)

      aaaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCta                                                                                   >  1:2384860/1‑93 (MQ=255)

       aaaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCtat                                                                                  >  2:3443823/1‑93 (MQ=255)

        aaTCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCtata                                                                                 >  1:6437897/1‑93 (MQ=255)

         atcatcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATaa                                                                                <  1:6846270/93‑1 (MQ=255)

          tcatcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAt                                                                               <  2:786330/93‑1 (MQ=255)

           catcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAtt                                                                              <  2:7683370/93‑1 (MQ=255)

           catcCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAtt                                                                              <  2:105089/93‑1 (MQ=255)

            ctcCTTTTTTATTTTTATTAAATAGTTGCGCGGTGCTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAAttt                                                                             <  1:6980508/92‑1 (MQ=255)

                ttttttATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTgtgt                                                                         >  1:4123919/1‑93 (MQ=255)

                   tttatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgc                                                                      <  2:7051845/93‑1 (MQ=255)

                   tttatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgc                                                                      >  1:7758679/1‑93 (MQ=255)

                    ttatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgct                                                                     >  2:1454883/1‑93 (MQ=255)

                     tatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgctg                                                                    >  2:4408951/1‑93 (MQ=255)

                     tatttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgctg                                                                    >  1:7823152/1‑93 (MQ=255)

                      atttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTtgctgc                                                                   >  1:3604969/1‑93 (MQ=255)

                        ttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCAt                                                                 <  2:4669/93‑1 (MQ=255)

                        ttttattAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCAt                                                                 <  2:2628608/93‑1 (MQ=255)

                        tgttattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCAt                                                                 <  1:1085614/91‑1 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTaa                                                              <  1:4375989/93‑1 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTaa                                                              <  1:5840583/93‑1 (MQ=255)

                           tattaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAAACCTACTCCCGTTACCTATAATTTGTGTTGCTGCATTaa                                                              >  1:4506089/1‑93 (MQ=255)

                            attaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAAt                                                             >  2:5713739/1‑93 (MQ=255)

                            attaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAAt                                                             >  1:6632687/1‑93 (MQ=255)

                             ttaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATa                                                            >  2:841655/1‑93 (MQ=255)

                             ttaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATa                                                            >  1:6337910/1‑93 (MQ=255)

                             ttaAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATa                                                            >  1:3277452/1‑93 (MQ=255)

                              taAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAg                                                           >  2:545054/1‑93 (MQ=255)

                               aaaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGc                                                          <  1:205796/93‑1 (MQ=255)

                               aaaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGc                                                          <  2:2970266/93‑1 (MQ=255)

                               aaaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGc                                                          >  2:4257026/1‑93 (MQ=255)

                               aaaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGc                                                          >  1:5037224/1‑93 (MQ=255)

                               aaaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGc                                                          >  1:4964591/1‑93 (MQ=255)

                                aaTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATCTGTGTTGCTGCATTAATAGCt                                                         <  1:5987867/93‑1 (MQ=255)

                                 aTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTGCCTATAATTTGTGTTGCTGCATTACTAGCtt                                                        >  1:5137935/1‑93 (MQ=255)

                                 aTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtt                                                        >  2:496816/1‑93 (MQ=255)

                                 aTAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtt                                                        >  1:3506951/1‑93 (MQ=255)

                                  tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       <  2:5218206/93‑1 (MQ=255)

                                  tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       >  2:3231320/1‑93 (MQ=255)

                                  tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       <  1:3218499/93‑1 (MQ=255)

                                  tAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       >  2:2779993/1‑93 (MQ=255)

                                  tAGTTGCGCGGCACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCtta                                                       <  2:4723302/93‑1 (MQ=255)

                                    gTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttatt                                                     >  2:1143158/1‑93 (MQ=255)

                                     ttGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCttatta                                                    >  1:6828865/1‑93 (MQ=255)

                                        cgcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataa                                                 <  2:3643349/93‑1 (MQ=255)

                                        cgcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataa                                                 >  1:1393353/1‑93 (MQ=255)

                                         gcgGTACTTTGTGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataat                                                >  2:5091491/1‑93 (MQ=255)

                                         gcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataat                                                >  2:6640091/1‑93 (MQ=255)

                                         gcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataat                                                >  1:7068528/1‑93 (MQ=255)

                                         gcgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataat                                                >  1:7168650/1‑93 (MQ=255)

                                          cgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataata                                               >  2:4099217/1‑93 (MQ=255)

                                          cgGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTataata                                               >  2:6550155/1‑93 (MQ=255)

                                            gTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATaaa                                             <  1:3718704/93‑1 (MQ=255)

                                              aCTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATc                                           <  1:4822859/93‑1 (MQ=255)

                                               cTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCa                                          <  2:3346438/93‑1 (MQ=255)

                                                tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAc                                         >  1:5972780/1‑93 (MQ=255)

                                                tttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAc                                         >  2:7581114/1‑93 (MQ=255)

                                                 ttATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCAcc                                        >  2:2457678/1‑93 (MQ=255)

                                                   aTGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCa                                      >  1:3823037/1‑93 (MQ=255)

                                                   aTGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCa                                      >  1:3428884/1‑93 (MQ=255)

                                                    tGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCaa                                     >  1:6283594/1‑93 (MQ=255)

                                                    tGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCaa                                     <  1:3080686/93‑1 (MQ=255)

                                                    tGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCaa                                     >  1:532475/1‑93 (MQ=255)

                                                     gTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAg                                    >  2:5203682/1‑93 (MQ=255)

                                                      ttCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGa                                   >  1:6227818/1‑93 (MQ=255)

                                                      ttCTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGa                                   >  1:5412455/1‑93 (MQ=255)

                                                        cTGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGaaa                                 <  2:7444295/93‑1 (MQ=255)

                                                         tGGCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAAt                                >  1:4795167/1‑93 (MQ=255)

                                                          ggCGCTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAAtt                               >  2:6344880/1‑93 (MQ=255)

                                                             gcTAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCAc                            >  2:3955936/1‑93 (MQ=255)

                                                               tAACACAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACtt                          >  1:1852891/1‑93 (MQ=255)

                                                                 acacAAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTcc                        >  2:3740876/1‑93 (MQ=255)

                                                                  cacaAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCct                       <  2:7159706/93‑1 (MQ=255)

                                                                  cacaAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCct                       <  1:1199703/93‑1 (MQ=255)

                                                                  cacaAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCct                       <  1:3922768/93‑1 (MQ=255)

                                                                    caAACCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCctct                     >  1:3653325/1‑93 (MQ=255)

                                                                     aaaCCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTa                    <  1:5896403/93‑1 (MQ=255)

                                                                       aCCAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTAtt                  >  1:7572623/1‑93 (MQ=255)

                                                                        ccAGAGCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTg                 <  2:1923023/93‑1 (MQ=255)

                                                                          agagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGtt               >  1:7201409/1‑93 (MQ=255)

                                                                           gagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTc              >  2:314449/1‑93 (MQ=255)

                                                                           gagCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTc              <  2:415390/93‑1 (MQ=255)

                                                                            agCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTct             <  2:6065779/93‑1 (MQ=255)

                                                                            agCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTct             >  1:1612274/1‑93 (MQ=255)

                                                                             gCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTcta            <  2:6465031/93‑1 (MQ=255)

                                                                             gCAACCCTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTcta            >  2:1243709/1‑93 (MQ=255)

                                                                                 cccTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTaa        >  1:2809580/1‑93 (MQ=255)

                                                                                   cTACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAAtt      <  1:2839950/93‑1 (MQ=255)

                                                                                    tACTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTg     <  2:6753039/93‑1 (MQ=255)

                                                                                     aCTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGc    >  2:3691723/1‑93 (MQ=255)

                                                                                      cTCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCt   >  2:2544746/1‑93 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  >  2:7199168/1‑93 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  <  1:2692761/93‑1 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  >  1:7446078/1‑93 (MQ=255)

                                                                                       tCCCGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCta  <  1:3297318/93‑1 (MQ=255)

CTAAGAAAAATCATCCTTTTTTATTTTTATTAAATAGTTGCGCGGTACTTTATGTTCTGGCGCTAACACAAACCAGAGCAACCCTACTCCTGTTCCCTATAATTTGTGTTGCTGCATTAATAGCTTATTATAATAAATCACCCAAGAAATTCACTTCCTCTATTGTTCTACTAATTGCTA  >  NC_000913/3795143‑3795322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`