BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,845,193	G→A	T354T (ACC→ACT)	uhpC ←	UhpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,845,193	0	G	A	99.4%	1224.0 / ‑4.5	342	T354T (ACC→ACT)	uhpC	UhpC
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (186/154); total (187/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

Only 100 of 346 total aligned reads displayed.

CCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAAT  >  NC_000913/3845103‑3845282

  ccGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTga                                                                                       >  2:286701/1‑93 (MQ=255)

  ccGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTga                                                                                       <  1:3697471/93‑1 (MQ=255)

  ccGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTga                                                                                       >  2:859628/1‑93 (MQ=255)

  ccGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTga                                                                                       <  2:6785506/93‑1 (MQ=255)

   cGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTgaa                                                                                      >  2:2802360/1‑93 (MQ=255)

     tCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTgaaga                                                                                    <  1:4185730/93‑1 (MQ=255)

     tCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTgaaga                                                                                    <  2:2066226/93‑1 (MQ=255)

     tCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTgaaga                                                                                    >  1:6556915/1‑93 (MQ=255)

     tCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTgaaga                                                                                    <  1:1006600/93‑1 (MQ=255)

       gccgccCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGaagaag                                                                                  >  2:7139674/1‑93 (MQ=255)

       gccgccCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGaagaag                                                                                  >  2:7426719/1‑93 (MQ=255)

          gccCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAg                                                                               >  1:3162065/1‑93 (MQ=255)

           ccccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGTTTAACATCTTTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAgt                                                                              >  1:2337137/1‑92 (MQ=255)

           ccccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAgg                                                                              >  1:4100167/1‑93 (MQ=255)

           ccccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAgg                                                                              >  1:15147/1‑93 (MQ=255)

            cccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGt                                                                             >  1:3371265/1‑93 (MQ=255)

            cccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGt                                                                             >  1:3884289/1‑93 (MQ=255)

            cccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGt                                                                             >  1:998368/1‑93 (MQ=255)

             ccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGtt                                                                            <  2:3393280/93‑1 (MQ=255)

             ccTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGtt                                                                            >  2:7808246/1‑93 (MQ=255)

              cTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTg                                                                           >  1:1650140/1‑93 (MQ=255)

              cTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTg                                                                           >  1:7536886/1‑93 (MQ=255)

              cTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTg                                                                           >  1:4892686/1‑93 (MQ=255)

                gccgccTCTTTGTGGGACCACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGcc                                                                         <  2:4146606/93‑1 (MQ=255)

                gccgccTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGcc                                                                         <  2:4441552/93‑1 (MQ=255)

                   gccTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGc                                                                      >  1:6198479/1‑93 (MQ=255)

                     ctctTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCAt                                                                    >  1:2831503/1‑93 (MQ=255)

                      tctTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATc                                                                   >  2:2661822/1‑93 (MQ=255)

                      tctTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATc                                                                   >  1:3228139/1‑93 (MQ=255)

                      tctTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATc                                                                   >  1:7189710/1‑93 (MQ=255)

                       ctTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCa                                                                  <  1:4576970/93‑1 (MQ=255)

                        tttGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCAc                                                                 <  2:1339157/93‑1 (MQ=255)

                           gtgGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTa                                                              <  1:5538981/93‑1 (MQ=255)

                            tgGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAg                                                             >  1:2434887/1‑93 (MQ=255)

                              ggAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCt                                                           <  2:959796/93‑1 (MQ=255)

                              ggAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCt                                                           <  1:2955655/93‑1 (MQ=255)

                               gAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTg                                                          <  1:6433681/93‑1 (MQ=255)

                               gAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTg                                                          <  1:6721823/93‑1 (MQ=255)

                               gAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTg                                                          <  2:3004535/93‑1 (MQ=255)

                                aaCACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTgg                                                         >  2:6332804/1‑93 (MQ=255)

                                 acacTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGc                                                        <  1:434675/93‑1 (MQ=255)

                                    ctctGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                     <  1:1070290/93‑1 (MQ=255)

                                    ctctGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                     <  2:5394748/93‑1 (MQ=255)

                                    ctctGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                     >  2:4958450/1‑93 (MQ=255)

                                    ctctGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                     >  2:3993761/1‑93 (MQ=255)

                                    ctctGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCaaa                                                     >  1:3804648/1‑93 (MQ=255)

                                      ctGCCGCCGCCATACCGATTAACATCTGTGGTCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATg                                                   <  1:3284032/93‑1 (MQ=255)

                                      ctGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATg                                                   <  1:7411900/93‑1 (MQ=255)

                                       tGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATgg                                                  >  2:2130384/1‑93 (MQ=255)

                                          cgccgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCAt                                               <  2:3847978/93‑1 (MQ=255)

                                           gccgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATc                                              >  2:347776/1‑93 (MQ=255)

                                           gccgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATc                                              >  2:5478322/1‑93 (MQ=255)

                                           gccgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATc                                              >  2:5971916/1‑93 (MQ=255)

                                           gccgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATc                                              <  1:3532970/93‑1 (MQ=255)

                                            ccgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCa                                             >  2:5719984/1‑93 (MQ=255)

                                             cgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAg                                            >  1:2076098/1‑93 (MQ=255)

                                             cgccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAg                                            <  2:6473679/93‑1 (MQ=255)

                                              gccATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGc                                           >  2:6038143/1‑93 (MQ=255)

                                                 aTACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcac                                        <  2:721119/93‑1 (MQ=255)

                                                 aTACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcac                                        >  2:1899663/1‑93 (MQ=255)

                                                  tACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcaca                                       >  1:7687928/1‑93 (MQ=255)

                                                  tACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcaca                                       >  1:4826530/1‑93 (MQ=255)

                                                  tACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcaca                                       >  1:2229131/1‑93 (MQ=255)

                                                  tACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCcaca                                       >  1:1896245/1‑93 (MQ=255)

                                                   aCCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAg                                      >  1:2985529/1‑93 (MQ=255)

                                                    ccGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAgg                                     >  2:1242062/1‑93 (MQ=255)

                                                      gATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGa                                   >  2:2459034/1‑93 (MQ=255)

                                                      gATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGa                                   <  2:1554763/93‑1 (MQ=255)

                                                      gATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGa                                   <  1:2197439/93‑1 (MQ=255)

                                                      gATTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGa                                   >  2:3548917/1‑93 (MQ=255)

                                                       aTTAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAg                                  >  1:6856276/1‑93 (MQ=255)

                                                        ttAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGc                                 >  1:6005452/1‑93 (MQ=255)

                                                        ttAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGc                                 >  2:1423298/1‑93 (MQ=255)

                                                         tAACATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGcc                                >  1:7070263/1‑93 (MQ=255)

                                                          aaCATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCg                               >  2:3033525/1‑93 (MQ=255)

                                                            cATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGAc                             >  2:4651245/1‑93 (MQ=255)

                                                            cATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGAc                             >  2:6902852/1‑93 (MQ=255)

                                                            cATCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGAc                             >  2:5573702/1‑93 (MQ=255)

                                                             aTCTGTGGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACt                            <  2:528476/93‑1 (MQ=255)

                                                                 gtgGGCCAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGaaa                        <  2:4892997/93‑1 (MQ=255)

                                                                 gtgGGCCAAAGACAAAAAAACCAATAGGGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGaaa                        <  2:3348318/93‑1 (MQ=255)

                                                                     gtcAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCaa                    <  2:5159957/91‑1 (MQ=255)

                                                                       cAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCaaaa                  >  1:5184369/1‑93 (MQ=255)

                                                                       cAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCaaaa                  >  1:2542067/1‑93 (MQ=255)

                                                                       cAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCaaaa                  >  2:658064/1‑93 (MQ=255)

                                                                       cAAAGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCaaaa                  <  1:3654120/93‑1 (MQ=255)

                                                                         aaGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAAtt                >  1:471539/1‑93 (MQ=255)

                                                                         aaGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAAtt                >  1:3490345/1‑93 (MQ=255)

                                                                          aGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTc               <  2:4763560/93‑1 (MQ=255)

                                                                          aGACAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTc               >  2:7816200/1‑93 (MQ=255)

                                                                            aCAAAAAAACCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCcg             >  2:7489173/1‑93 (MQ=255)

                                                                              aaaaaacCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCcggc           <  1:544998/93‑1 (MQ=255)

                                                                              aaaaaaaCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGATCCGACTGAAAGCAAAATTCcggc           >  2:4313588/1‑93 (MQ=255)

                                                                              aaaaaaaCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCcggc           <  1:5650936/93‑1 (MQ=255)

                                                                                aaaaaCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCcggcgg         >  2:164847/1‑93 (MQ=255)

                                                                                aaaaaCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCcggcgg         >  1:7915953/1‑93 (MQ=255)

                                                                                    aCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGaa     >  1:6050016/1‑93 (MQ=255)

                                                                                    aCCAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGaa     >  1:7260082/1‑93 (MQ=255)

                                                                                     ccAATAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGaaa    >  1:4471803/1‑93 (MQ=255)

                                                                                       aaTAGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAAt  >  2:1178309/1‑93 (MQ=255)

CCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAAT  >  NC_000913/3845103‑3845282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`