BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,876,595	G→C	P385A (CCG→GCG)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,876,595	0	G	C	99.5%	673.1 / NA	216	P385A (CCG→GCG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (1/0); new base C (38/177); total (39/177)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

GGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGGCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTT  >  NC_000913/3876504‑3876684

  aTCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCNNTTTCCGCGCGCGACGCGcngc                                                                                        >  1:932279/1‑93 (MQ=255)

  aTCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGcgccgc                                                                                        >  2:7292813/1‑93 (MQ=255)

   tCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCt                                                                                       <  2:430704/93‑1 (MQ=255)

    cgcgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGCGCGACGcgccgcct                                                                                      >  2:3342354/1‑91 (MQ=255)

     gcgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTc                                                                                     <  2:6659516/93‑1 (MQ=255)

     gcgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTc                                                                                     <  1:2064183/93‑1 (MQ=255)

     gcgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTc                                                                                     <  1:3152079/93‑1 (MQ=255)

      cgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTca                                                                                    <  1:6469685/93‑1 (MQ=255)

       gTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACCCGCGCGACGCGCCGCCTcac                                                                                   >  2:2613181/1‑93 (MQ=255)

        ttCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacg                                                                                  <  1:6201749/93‑1 (MQ=255)

        tgccTGGCAGCCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacg                                                                                  <  2:115471/91‑1 (MQ=255)

         tCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACCCGCGCGGCGGGCCGCTTcacgg                                                                                 >  2:5495090/1‑93 (MQ=255)

           cTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTAAGCGCGCGACGCGCCGCCTcacgcgc                                                                               >  2:5137737/1‑93 (MQ=255)

            tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggca                                                                              <  1:6577334/93‑1 (MQ=255)

            tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggca                                                                              <  1:3955010/93‑1 (MQ=255)

            tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTTACCCGCCCCCCGCGCCCCCTcccgggcc                                                                              >  2:3448599/1‑92 (MQ=255)

            tGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGTGCGGGACGCGCCGCTTcacgggcc                                                                              >  1:6748167/1‑92 (MQ=255)

              gCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggcacg                                                                            <  1:3033636/93‑1 (MQ=255)

               cAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggcacgg                                                                           <  1:2867959/93‑1 (MQ=255)

               cAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGGGCGGCGCGCCGCTTcacgggcacgg                                                                           >  1:2300691/1‑93 (MQ=255)

                aGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTcacgggcacggg                                                                          >  1:3347743/1‑93 (MQ=255)

                  tCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTTACGCGCGCGACGCGCCGCTTCAcgggcacgggca                                                                        >  2:5554597/1‑93 (MQ=255)

                  tCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCAcgggcacgggca                                                                        <  1:3102814/93‑1 (MQ=255)

                  tCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCAcgggcacgggca                                                                        <  2:3027938/93‑1 (MQ=255)

                   cTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTCACGCGCCCGGCGCGCCGCTTCcccggcccgggccg                                                                       >  1:1659580/1‑91 (MQ=255)

                   cTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGCGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcag                                                                       >  1:2695743/1‑93 (MQ=255)

                    tGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGCCGCGCCGCTTCACGGGCACGGgcagc                                                                      >  1:7163067/1‑93 (MQ=255)

                    tGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcagc                                                                      <  1:3284334/93‑1 (MQ=255)

                    tGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCCTTACGGCGGGGCCTTTTACGCGCGGCACGCGCCGCCTGCCGGGCAGGGgcagc                                                                      >  2:2325254/1‑93 (MQ=255)

                     gCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcagca                                                                     <  2:2671427/93‑1 (MQ=255)

                     gCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACGCGCGCGGCGCGCCGCCTCACGGGGACCGgccgca                                                                     >  2:5322568/1‑93 (MQ=255)

                      ccAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCAt                                                                    <  2:1933067/93‑1 (MQ=255)

                         gTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGa                                                                 <  2:6729128/93‑1 (MQ=255)

                         gTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGa                                                                 <  1:1005863/93‑1 (MQ=255)

                          tttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTACCCGCGCGGCGCGCCGCTTCACGGGCACGGGCAGCATCGAt                                                                >  2:6061394/1‑93 (MQ=255)

                           ttGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACCGGCAGCATCGATa                                                               >  1:1177564/1‑93 (MQ=255)

                            tGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATaa                                                              <  1:5207342/93‑1 (MQ=255)

                            tGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTAACGGGCACGGGCAGCATCGATaa                                                              <  1:5738990/93‑1 (MQ=255)

                            tGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGGAGCATCCATaa                                                              >  2:352936/1‑93 (MQ=255)

                              cccGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCGTCGATAAtt                                                            <  1:5880998/93‑1 (MQ=255)

                              cccGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAAtt                                                            <  1:7635043/93‑1 (MQ=255)

                                 ggCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTTTCGCGCGCGGGGCGCCGCTTTTCCGGCGCGGGCAGCGTCGAGAAATTTg                                                         >  1:4453658/1‑93 (MQ=255)

                                  gCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGGCGCCCCGCTTCACGGGGACCGGCCGCCTCGGTTATTTTGc                                                        >  1:5081495/1‑93 (MQ=255)

                                      gCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACCGGCCGCCTCGATAATTTTTcccaa                                                    >  2:4475566/1‑90 (MQ=255)

                                        ccGCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGGAGCATCCATAATTTTGCCAACCa                                                  >  1:2389474/1‑93 (MQ=255)

                                        ccGCTAAGTCGAGCGCACCTTGACGGCGGGTCATTTCACGTGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCa                                                  <  2:2824330/93‑1 (MQ=255)

                                         cGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCAc                                                 <  2:5752716/93‑1 (MQ=255)

                                          gCTAAGTCGAGCGCACCTTTACGGCGGGGCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCAct                                                >  1:2144374/1‑92 (MQ=255)

                                           cTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGa                                               <  2:3793155/93‑1 (MQ=255)

                                           cTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGa                                               <  2:1338920/93‑1 (MQ=255)

                                                tCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTc                                          <  1:5346678/93‑1 (MQ=255)

                                                     cgcACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGTAGCATCGATAATTTTGCCAACCACGATTTTCGCGTc                                     <  2:6849149/93‑1 (MQ=255)

                                                      gcACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGGCCACCACCATTTTCgcggcg                                    >  2:5379630/1‑93 (MQ=255)

                                                        cccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGt                                  <  1:599037/92‑1 (MQ=255)

                                                         ccTTTTGGGCGGGTTATTCCCCGCGCGCGGCGCTCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGtt                                 <  2:2073925/93‑1 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCGCGCGCGCGGCGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGtt                                 <  1:4198524/93‑1 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTTGCGTCGGtt                                 >  2:4138701/1‑93 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTTGCGTCGGtt                                 >  2:894834/1‑93 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCTCGTCGGtt                                 >  1:7389267/1‑93 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGtt                                 >  2:4755261/1‑93 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGtt                                 <  2:6390255/93‑1 (MQ=255)

                                                         ccTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGtt                                 <  1:7889269/93‑1 (MQ=255)

                                                          cTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCCCGGGCACGGGCAGCATCGATAATTTTGCCAACCACGCTTTTTGCGTCGGGTg                                >  2:6117617/1‑93 (MQ=255)

                                                             tACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGt                             <  2:5236724/93‑1 (MQ=255)

                                                             tACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGt                             <  2:703766/93‑1 (MQ=255)

                                                              aCGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGtt                            <  2:2451426/93‑1 (MQ=255)

                                                               cggcggGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGttt                           <  1:6276073/93‑1 (MQ=255)

                                                               cggcggGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAAATTTTCCAACCACGATTTTTGCGTCGGTTGGGgtt                           >  2:7725262/1‑93 (MQ=255)

                                                                 gcggGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTc                         <  1:5501438/93‑1 (MQ=255)

                                                                  cggGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTcc                        <  2:5360021/93‑1 (MQ=255)

                                                                   gggTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCca                       >  1:1538953/1‑93 (MQ=255)

                                                                   gggTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCca                       <  1:1318921/93‑1 (MQ=255)

                                                                   gggTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCca                       >  1:4073639/1‑93 (MQ=255)

                                                                    ggTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcag                      >  2:487895/1‑93 (MQ=255)

                                                                     gTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagc                     <  2:1689600/93‑1 (MQ=255)

                                                                     gTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagc                     >  2:117565/1‑93 (MQ=255)

                                                                      tCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                    >  1:6946142/1‑93 (MQ=255)

                                                                      gcATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                    <  1:1802784/92‑1 (MQ=255)

                                                                       cATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagcag                   <  2:485483/93‑1 (MQ=255)

                                                                       cATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagcag                   <  1:3903059/93‑1 (MQ=255)

                                                                        aTTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAgg                  >  1:7572680/1‑93 (MQ=255)

                                                                        aTTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAgg                  >  2:7188968/1‑93 (MQ=255)

                                                                        aTTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAgg                  >  2:3669395/1‑93 (MQ=255)

                                                                         tttCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGt                 >  1:7279805/1‑93 (MQ=255)

                                                                         tttCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGt                 <  1:1274619/93‑1 (MQ=255)

                                                                         tttCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATAGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGt                 >  1:3458607/1‑93 (MQ=255)

                                                                            cACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTAtt              <  1:5624810/93‑1 (MQ=255)

                                                                            cACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTAtt              <  2:3847039/93‑1 (MQ=255)

                                                                             ccgcgcgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTc             <  1:4719353/92‑1 (MQ=255)

                                                                             ccgcgcgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTc             <  1:1879827/92‑1 (MQ=255)

                                                                             aCGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTc             >  2:6200646/1‑93 (MQ=255)

                                                                                 gcgcgACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGcc         <  2:7458787/93‑1 (MQ=255)

                                                                                     gACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagca     <  2:1510212/93‑1 (MQ=255)

                                                                                     gACGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagca     <  2:7596887/93‑1 (MQ=255)

                                                                                      gcgcgCCGCTTCACGGGCGCGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagcag    <  1:3571525/92‑1 (MQ=255)

                                                                                      aCGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagcag    <  1:2254140/93‑1 (MQ=255)

                                                                                      aCGCGCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagcag    >  2:404359/1‑93 (MQ=255)

                                                                                       cgcgCCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgt   <  1:4971950/93‑1 (MQ=255)

                                                                                        gcgccgCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgtt  <  2:6910524/93‑1 (MQ=255)

                                                                                        gcgccgCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgtt  >  2:521566/1‑93 (MQ=255)

GGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGGCGCTTCACGGGCACGGGCAGCATCGATAATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTT  >  NC_000913/3876504‑3876684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`