BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	436,659	T→C	E258G (GAG→GGG)	yajO ←	putative NAD(P)H‑dependent xylose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,659	0	T	C	100.0%	704.2 / NA	222	E258G (GAG→GGG)	yajO	putative NAD(P)H‑dependent xylose reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (124/98); total (124/98)

Only 100 of 236 total aligned reads displayed.

TGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCC  >  NC_000913/436568‑436748

 gtCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCCCCcca                                                                                         <  2:483011/93‑1 (MQ=255)

  tCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCccat                                                                                        <  1:4319937/93‑1 (MQ=255)

  tCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCccat                                                                                        <  1:625200/93‑1 (MQ=255)

  tCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCccat                                                                                        <  1:7104314/93‑1 (MQ=255)

    gCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCccatca                                                                                      <  2:1446816/93‑1 (MQ=255)

      ccccAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGa                                                                                    <  2:4674920/93‑1 (MQ=255)

      ccccAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGa                                                                                    >  2:2354434/1‑93 (MQ=255)

         cAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGacac                                                                                 <  2:5173773/93‑1 (MQ=255)

          aGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACAcc                                                                                >  1:1635939/1‑93 (MQ=255)

          aGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACAcc                                                                                >  1:4858043/1‑93 (MQ=255)

           gTTTTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCa                                                                               <  1:1985767/93‑1 (MQ=255)

            ttcttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAg                                                                              >  1:6389580/1‑93 (MQ=255)

              cttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTc                                                                            <  2:2865996/93‑1 (MQ=255)

               ttcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCg                                                                           >  2:2759661/1‑93 (MQ=255)

                tcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGt                                                                          >  1:6796156/1‑93 (MQ=255)

                tcACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGt                                                                          <  1:318955/93‑1 (MQ=255)

                 cACTGACGCCTGTTAACCGCTGTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTg                                                                         <  2:1631102/93‑1 (MQ=255)

                 cACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTg                                                                         <  2:758093/93‑1 (MQ=255)

                  aCTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGc                                                                        <  1:225765/93‑1 (MQ=255)

                     gACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGt                                                                     >  2:7004862/1‑93 (MQ=255)

                      aCGCCTGTTAACCGCTCTGCGATCTGTGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTa                                                                    >  2:3012470/1‑93 (MQ=255)

                        gCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGt                                                                  >  1:7737668/1‑93 (MQ=255)

                        gCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGt                                                                  >  1:548661/1‑93 (MQ=255)

                         ccTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGtt                                                                 >  2:3849182/1‑93 (MQ=255)

                           tGTTAACCGCTCTGCGATCTGCGCGTTTTTTTCATCGCTTTCTTTATAGCGATTTTTCCCCACCTCATCAGACACCAGTCGAGCGGTAGCTtc                                                               >  2:2693991/1‑93 (MQ=255)

                           tGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtc                                                               >  2:3482509/1‑93 (MQ=255)

                           tGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtc                                                               <  2:4868529/93‑1 (MQ=255)

                           tGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCAACCCATCAGACACCAGTCGTGCGGTAGTTtc                                                               >  1:1570439/1‑93 (MQ=255)

                            gTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtct                                                              <  2:1805874/93‑1 (MQ=255)

                            gTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtct                                                              <  2:6554244/93‑1 (MQ=255)

                            gTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTtct                                                              <  1:1307853/93‑1 (MQ=255)

                              tAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTcc                                                            <  2:1223007/93‑1 (MQ=255)

                               taCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTGCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTccc                                                           <  1:240806/92‑1 (MQ=255)

                               aaCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTccc                                                           >  2:7442269/1‑93 (MQ=255)

                                aCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTcccc                                                          >  2:7064386/1‑93 (MQ=255)

                                   gCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACg                                                       >  2:6048414/1‑93 (MQ=255)

                                   gCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACg                                                       >  1:6505293/1‑93 (MQ=255)

                                    ctctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACgg                                                      >  1:6751589/1‑93 (MQ=255)

                                     tctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCGCCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGa                                                     <  1:5110539/93‑1 (MQ=255)

                                     tctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGa                                                     <  2:7804465/93‑1 (MQ=255)

                                      ctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACCGAc                                                    >  1:5287371/1‑93 (MQ=255)

                                       tGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGAcg                                                   >  2:243088/1‑93 (MQ=255)

                                           aTCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTc                                               >  2:3770922/1‑93 (MQ=255)

                                           aTCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCACCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTc                                               >  2:2643351/1‑93 (MQ=255)

                                            tCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCa                                              <  2:6202551/93‑1 (MQ=255)

                                            tCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCa                                              <  2:4160389/93‑1 (MQ=255)

                                             cTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAg                                             >  2:6740135/1‑93 (MQ=255)

                                              tGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGa                                            <  1:2704214/93‑1 (MQ=255)

                                               gcgcGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGAc                                           <  1:6123033/93‑1 (MQ=255)

                                               gcgcGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGAc                                           >  1:1493902/1‑93 (MQ=255)

                                               gcgcGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGAc                                           <  1:4814808/93‑1 (MQ=255)

                                                 gcgTCATTTTCGTCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACgg                                         >  2:2332265/1‑93 (MQ=255)

                                                 gcgTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACgg                                         <  2:3411225/93‑1 (MQ=255)

                                                  cgTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCCCCCCACGGACGCGTCAGACGGc                                        <  2:842073/93‑1 (MQ=255)

                                                   gTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcc                                       >  2:518112/1‑93 (MQ=255)

                                                   gTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcc                                       <  2:5356684/93‑1 (MQ=255)

                                                   gTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcc                                       >  1:5555373/1‑93 (MQ=255)

                                                    tCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGccc                                      >  1:1534781/1‑93 (MQ=255)

                                                     cATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGTTAGTTTCTCCCCACGGACGCGTCAGACGGcccc                                     <  2:790950/93‑1 (MQ=255)

                                                     cATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGcccc                                     <  1:7322045/93‑1 (MQ=255)

                                                      aTTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCt                                    >  1:7576405/1‑93 (MQ=255)

                                                      aTTTTCATCGCTTTCTTTATAGAGATTGTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCt                                    <  1:7153378/93‑1 (MQ=255)

                                                       ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                   >  1:4624828/1‑93 (MQ=255)

                                                       ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                   >  2:6437189/1‑93 (MQ=255)

                                                       ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                   >  1:6583513/1‑93 (MQ=255)

                                                       ttttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                   <  1:1423500/93‑1 (MQ=255)

                                                        tttCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTg                                  >  2:6609508/1‑93 (MQ=255)

                                                          tCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGATGCGTCAGACGGCCCCTTGcc                                >  1:4694693/1‑93 (MQ=255)

                                                          tCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGcc                                >  2:1962260/1‑93 (MQ=255)

                                                          tCATCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGcc                                >  2:731028/1‑93 (MQ=255)

                                                             tCGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGc                             <  2:1178280/93‑1 (MQ=255)

                                                              cGCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCg                            >  2:6973000/1‑93 (MQ=255)

                                                               gCTTTCTTTATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCgg                           <  1:3408644/93‑1 (MQ=255)

                                                                 tttctttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGc                         <  2:6281976/93‑1 (MQ=255)

                                                                  ttctttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCt                        >  1:5994348/1‑93 (MQ=255)

                                                                     tttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCa                     <  1:5349582/93‑1 (MQ=255)

                                                                     tttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCa                     <  1:3671037/93‑1 (MQ=255)

                                                                     tttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCa                     >  1:1981065/1‑93 (MQ=255)

                                                                     tttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCa                     <  2:2895190/93‑1 (MQ=255)

                                                                      ttATAGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCAt                    >  1:4053868/1‑93 (MQ=255)

                                                                        ataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGTCCCCTTGCCAGCGGGCTCCATgg                  >  1:1956470/1‑93 (MQ=255)

                                                                        ataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATgg                  >  2:5832910/1‑93 (MQ=255)

                                                                        ataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATgg                  >  2:2144587/1‑93 (MQ=255)

                                                                        ataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATgg                  >  1:4938735/1‑93 (MQ=255)

                                                                        ataGAGATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATgg                  >  2:1697264/1‑93 (MQ=255)

                                                                          agagATTTTTCCCCGCCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGaa                <  1:3820153/93‑1 (MQ=255)

                                                                          agagATTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGaa                <  2:4237391/93‑1 (MQ=255)

                                                                            agaTTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAAtt              <  1:1282719/93‑1 (MQ=255)

                                                                             gaTTTTTCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGCAATTa             >  1:2977091/1‑93 (MQ=255)

                                                                              aTTTTTCCCCACCCCATCAGACACCAGGCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAc            <  1:51774/93‑1 (MQ=255)

                                                                               tttttCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAcc           <  1:5800538/93‑1 (MQ=255)

                                                                               tttttCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTAcc           <  1:7510468/93‑1 (MQ=255)

                                                                                 tttCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGc         >  2:5610551/1‑93 (MQ=255)

                                                                                  ttCCCCACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc        <  1:1252785/93‑1 (MQ=255)

                                                                                    ccccccccCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCAc      <  2:3621305/93‑1 (MQ=255)

                                                                                    ccccaccccaTCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCAc      <  1:1348051/93‑1 (MQ=255)

                                                                                     cccACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACg     >  2:886262/1‑93 (MQ=255)

                                                                                       cACCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGcc   >  1:2883911/1‑93 (MQ=255)

                                                                                        aCCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGccc  >  2:1162083/1‑93 (MQ=255)

                                                                                        aCCCCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGGCAGACGGTCCCTTGCCAGCGGGCTCCATAGGATGACCGCGACGccc  >  1:838379/1‑93 (MQ=255)

TGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCC  >  NC_000913/436568‑436748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`