BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	439,695	A→G	Y211H (TAT→CAT)	ispA ←	geranyltranstransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	439,695	0	A	G	99.4%	980.5 / ‑4.5	313	Y211H (TAT→CAT)	ispA	geranyltranstransferase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (174/136); total (175/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 320 total aligned reads displayed.

ACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTT  >  NC_000913/439606‑439784

aCCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCtt                                                                                        >  2:7074042/1‑93 (MQ=255)

aCCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCtt                                                                                        >  1:3687553/1‑93 (MQ=255)

 cccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTg                                                                                       >  1:6733176/1‑93 (MQ=255)

 cccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTg                                                                                       <  1:6159231/93‑1 (MQ=255)

 cccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTg                                                                                       <  2:1099114/93‑1 (MQ=255)

  ccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGt                                                                                      >  2:5100748/1‑93 (MQ=255)

   cTGGCGTTTTCCCGACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTc                                                                                     >  1:5229044/1‑93 (MQ=255)

      gCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAg                                                                                  >  1:3358020/1‑93 (MQ=255)

         ttttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTAc                                                                               >  2:7356461/1‑93 (MQ=255)

         ttttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTAc                                                                               >  2:6540123/1‑93 (MQ=255)

          tttCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTAcc                                                                              <  1:7793673/93‑1 (MQ=255)

              ccAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCa                                                                          >  2:4667408/1‑93 (MQ=255)

              ccAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCa                                                                          >  1:2924316/1‑93 (MQ=255)

              ccAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCa                                                                          <  1:3222319/93‑1 (MQ=255)

               cAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCag                                                                         >  2:2005285/1‑93 (MQ=255)

                 aCGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCagag                                                                       >  1:1073094/1‑93 (MQ=255)

                  cGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGc                                                                      <  2:2190934/93‑1 (MQ=255)

                    ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    <  2:1065764/93‑1 (MQ=255)

                    ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    <  1:7247427/93‑1 (MQ=255)

                    ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    >  1:6504648/1‑93 (MQ=255)

                    ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    >  2:5990043/1‑93 (MQ=255)

                    ttGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCac                                                                    <  2:58651/93‑1 (MQ=255)

                     tGCAGTATCTCCCACCACATCGAGGATGTCATCCTGAACCTGGAAGGCAAGGCTGATGCGCTCTGCATTCTTGTCGAGTACCGGCAGAGCacg                                                                   <  2:1722570/93‑1 (MQ=255)

                      gCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCacga                                                                  <  1:7645381/93‑1 (MQ=255)

                          tATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTc                                                              >  2:6781265/1‑93 (MQ=255)

                            tctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCt                                                            <  2:6091325/93‑1 (MQ=255)

                             ctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                           <  1:4340285/93‑1 (MQ=255)

                             ctcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                           >  2:265583/1‑93 (MQ=255)

                              tcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                          <  1:4803840/93‑1 (MQ=255)

                              tcCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                          <  2:3205175/93‑1 (MQ=255)

                               cccACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCtttt                                                         >  1:5774429/1‑92 (MQ=255)

                                ccaccaCATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAt                                                        <  1:5010015/93‑1 (MQ=255)

                                ccaccaCATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAt                                                        <  2:3599028/93‑1 (MQ=255)

                                 caccacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtc                                                       >  1:1955536/1‑93 (MQ=255)

                                  accacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                                      >  2:307813/1‑93 (MQ=255)

                                  accacATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                                      >  1:4175850/1‑93 (MQ=255)

                                    cacaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                                    >  1:7446447/1‑93 (MQ=255)

                                    cacaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTcc                                                    >  2:860844/1‑93 (MQ=255)

                                    cacaGCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAACACGACGTCCTTTATCTcc                                                    <  2:1928695/93‑1 (MQ=255)

                                     acaTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                                   >  1:6920655/1‑93 (MQ=255)

                                      caTCCAGGCTGTCATCCTGACCCCGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTCCCGGCAGAGGGGCACGTCCTATACCTCCgg                                                  <  2:5347207/93‑1 (MQ=255)

                                      caTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                                  <  1:6617757/93‑1 (MQ=255)

                                      caTCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                                  >  1:2335158/1‑93 (MQ=255)

                                        tCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcg                                                >  1:376017/1‑93 (MQ=255)

                                        tCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcg                                                >  2:4090019/1‑93 (MQ=255)

                                          cAGGATGTCATCCTGAACGTGGAAGGCGAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCGTTTATCTCCGGCGCt                                              >  2:7916083/1‑93 (MQ=255)

                                          cAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCt                                              >  2:6313743/1‑93 (MQ=255)

                                            ggATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                            <  1:557560/93‑1 (MQ=255)

                                            ggATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                            >  1:6258137/1‑93 (MQ=255)

                                               tGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATg                                         >  1:2692860/1‑93 (MQ=255)

                                                gTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGc                                        <  2:6875940/93‑1 (MQ=255)

                                                gTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGc                                        <  1:1732441/93‑1 (MQ=255)

                                                 tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGcc                                       >  1:2641953/1‑92 (MQ=255)

                                                 tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                       >  1:7170725/1‑93 (MQ=255)

                                                 tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                       <  1:312342/93‑1 (MQ=255)

                                                 tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                       >  2:4463272/1‑93 (MQ=255)

                                                 tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                       >  2:4509451/1‑93 (MQ=255)

                                                 tCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                       <  2:7823630/93‑1 (MQ=255)

                                                   aTCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCAcc                                     <  1:7111239/93‑1 (MQ=255)

                                                   aTCCTGAAACTGGAAGGCAAGGCAGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACTTCCTTTATCTCCGGCGCTTAATGCAcc                                     <  2:5408380/93‑1 (MQ=255)

                                                    tCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCa                                    <  1:463017/93‑1 (MQ=255)

                                                     ccggAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  1:6415084/90‑1 (MQ=255)

                                                     ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  1:1016708/93‑1 (MQ=255)

                                                     ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  2:3997484/93‑1 (MQ=255)

                                                     ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   >  2:2769412/1‑93 (MQ=255)

                                                     ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  2:2558120/93‑1 (MQ=255)

                                                     ccTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCaa                                   <  1:4571699/93‑1 (MQ=255)

                                                      cTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAg                                  <  2:7650119/93‑1 (MQ=255)

                                                      cTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAg                                  <  2:1632268/93‑1 (MQ=255)

                                                       tGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAgg                                 >  2:3892764/1‑93 (MQ=255)

                                                        gAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGc                                <  1:447833/93‑1 (MQ=255)

                                                        gAACCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGc                                <  2:1258699/93‑1 (MQ=255)

                                                         aaCCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCg                               >  1:1358961/1‑93 (MQ=255)

                                                         aaCCTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCg                               >  2:2458083/1‑93 (MQ=255)

                                                            cTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                            >  1:3515036/1‑93 (MQ=255)

                                                            cTGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAc                            >  2:2298370/1‑93 (MQ=255)

                                                             tGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                           >  1:883700/1‑93 (MQ=255)

                                                             tGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                           >  1:7848873/1‑93 (MQ=255)

                                                             tGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                           >  2:5235733/1‑93 (MQ=255)

                                                             tGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                           >  2:4813463/1‑93 (MQ=255)

                                                             tGGAAGGCAAGGCCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcg                           >  1:6705430/1‑93 (MQ=255)

                                                                  ggcaaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACggcggc                      >  2:2712253/1‑93 (MQ=255)

                                                                     aaggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGcgcg                   <  1:6100492/93‑1 (MQ=255)

                                                                       ggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGaa                 <  2:7143867/93‑1 (MQ=255)

                                                                       ggcCGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGaa                 <  1:1499978/93‑1 (MQ=255)

                                                                          cGATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCCAATCa              >  1:1184609/1‑93 (MQ=255)

                                                                           gagtctctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTACCTCCGGCGCTTATTGCACCAAGGCGAACGGCGGCGCGAATCaa             <  2:1605385/89‑1 (MQ=255)

                                                                           gATGCTCTCTGCATGCTTGTCGAGTACCGGCAGTGCACGACGTCCTTTATCTCCGGCGCTTAATGCACTAAGGAGAACGGCGGCGCGCATCaa             >  1:4073611/1‑93 (MQ=255)

                                                                           gATGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCaa             >  1:6094827/1‑93 (MQ=255)

                                                                             tGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATg           >  2:5460478/1‑93 (MQ=255)

                                                                             tGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATg           >  2:3951839/1‑93 (MQ=255)

                                                                             tGCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGCCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATg           >  1:2373904/1‑93 (MQ=255)

                                                                              gCTCTCTGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgc          <  2:115154/93‑1 (MQ=255)

                                                                                tctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc        >  2:3329831/1‑93 (MQ=255)

                                                                                 ctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCGGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGcc       <  1:5941133/93‑1 (MQ=255)

                                                                                 ctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGcc       <  2:5735091/93‑1 (MQ=255)

                                                                                 ctctGCATGCTTGTCGAGTACCGGCAGAGCACGACGCCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGcc       <  1:2212822/93‑1 (MQ=255)

                                                                                  tctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGCATCAATGCGCCg      >  1:5267358/1‑93 (MQ=255)

                                                                                  tctGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCg      >  1:4731025/1‑93 (MQ=255)

                                                                                    tGCATGCTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGt    >  2:7586467/1‑93 (MQ=255)

ACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTT  >  NC_000913/439606‑439784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`