BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,742	T→C	A320A (GCT→GCC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,742	0	T	C	99.2%	1120.8 / ‑5.3	359	A320A (GCT→GCC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (184/172); total (187/172)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 374 total aligned reads displayed.

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

  tCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGccgctg                                                                                       >  2:3559457/1‑93 (MQ=255)

   gggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGt                                                                                      <  1:5407943/92‑1 (MQ=255)

   cggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGt                                                                                      <  1:5266719/93‑1 (MQ=255)

    ggcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGtt                                                                                     >  1:2461646/1‑93 (MQ=255)

     gcggcggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTc                                                                                    >  1:5586593/1‑93 (MQ=255)

         cggTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCga                                                                                <  1:5964051/93‑1 (MQ=255)

           gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgagg                                                                              >  2:5895707/1‑92 (MQ=255)

           gTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgaga                                                                              >  1:71281/1‑93 (MQ=255)

           gTATCGGCCAGTCTCGTTTGACTATGCTGCTGATGCAACTGCCGCATATCGGACAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCgaga                                                                              <  1:7037284/93‑1 (MQ=255)

            tATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGagag                                                                             >  1:7812661/1‑93 (MQ=255)

            tATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGagag                                                                             >  1:1709363/1‑93 (MQ=255)

               cGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGACAGCCGCTGGTCGCGAGAGCGt                                                                          >  1:4836671/1‑93 (MQ=255)

                ggCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTc                                                                         <  2:3322189/93‑1 (MQ=255)

                ggCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTc                                                                         <  1:2805850/93‑1 (MQ=255)

                 gCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTcc                                                                        >  2:718902/1‑93 (MQ=255)

                 gCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTAGCGAGAGCGTcc                                                                        >  2:7444350/1‑93 (MQ=255)

                  ccAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTccc                                                                       <  2:2244582/93‑1 (MQ=255)

                  ccAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTccc                                                                       <  1:2729298/93‑1 (MQ=255)

                    aGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCtt                                                                     >  1:4349771/1‑93 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  1:6386346/93‑1 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  2:5780149/93‑1 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    <  1:754150/93‑1 (MQ=255)

                     gTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtc                                                                    >  1:6238467/1‑93 (MQ=255)

                      tctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtct                                                                   <  1:2309572/93‑1 (MQ=255)

                      tctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtct                                                                   >  2:3863930/1‑93 (MQ=255)

                       ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtctc                                                                  >  2:2999307/1‑93 (MQ=255)

                       ctcGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTtctc                                                                  >  2:1918036/1‑93 (MQ=255)

                         cGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctg                                                                <  2:6907787/93‑1 (MQ=255)

                         cGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctg                                                                >  2:4624005/1‑93 (MQ=255)

                          gTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgc                                                               <  2:7024977/93‑1 (MQ=255)

                          gTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgc                                                               >  2:7598376/1‑93 (MQ=255)

                           tttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgct                                                              >  1:2096079/1‑93 (MQ=255)

                            ttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgctg                                                             >  1:7424091/1‑93 (MQ=255)

                            ttGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTctgctg                                                             >  1:5432017/1‑93 (MQ=255)

                              gACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGta                                                           >  2:1824423/1‑93 (MQ=255)

                              gACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGta                                                           <  2:7389412/93‑1 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          >  1:4475834/1‑93 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          >  2:1482866/1‑93 (MQ=255)

                               aCTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaa                                                          >  2:2418614/1‑93 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        >  2:1306659/1‑93 (MQ=255)

                                 tATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaata                                                        <  2:7843011/93‑1 (MQ=255)

                                  aTGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaataa                                                       <  1:2916214/93‑1 (MQ=255)

                                  aTGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGtaataa                                                       <  1:3650666/93‑1 (MQ=255)

                                   tgctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTaataat                                                      >  1:2626147/1‑93 (MQ=255)

                                    gctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAtt                                                     <  1:2995818/93‑1 (MQ=255)

                                     ctgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAAttt                                                    <  1:834288/93‑1 (MQ=255)

                                      tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   >  1:3296778/1‑93 (MQ=255)

                                      tgctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTa                                                   >  2:1581644/1‑93 (MQ=255)

                                        ctgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATc                                                 <  2:278969/93‑1 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                <  2:5593808/93‑1 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                <  2:2412306/93‑1 (MQ=255)

                                         tgctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcg                                                >  1:4239738/1‑93 (MQ=255)

                                           ctgcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgcc                                              <  2:6730415/93‑1 (MQ=255)

                                             gcAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATcgccgc                                            <  2:6151226/93‑1 (MQ=255)

                                              cAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCgccgcc                                           <  2:1236783/93‑1 (MQ=255)

                                                aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                         >  2:6113236/1‑93 (MQ=255)

                                                aCTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAg                                         <  2:308957/93‑1 (MQ=255)

                                                 cTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGc                                        <  1:3534979/93‑1 (MQ=255)

                                                   gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGt                                      >  2:7100059/1‑93 (MQ=255)

                                                   gCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGt                                      <  1:6956241/93‑1 (MQ=255)

                                                     cGCATGTCGGCCCGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCt                                    <  1:3999003/93‑1 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  2:390739/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  1:21773/1‑93 (MQ=255)

                                                      gCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTg                                   >  2:4161570/1‑93 (MQ=255)

                                                       cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGa                                  <  2:1732080/93‑1 (MQ=255)

                                                       cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGa                                  >  1:2000679/1‑93 (MQ=255)

                                                       cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGa                                  >  2:2824838/1‑93 (MQ=255)

                                                       cATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAACGTCCCTTCTCTGCTGTAATAATTTATCCTCGCCAGCGCCTGa                                  >  1:387340/1‑93 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 <  1:487521/93‑1 (MQ=255)

                                                        atatCGGCCAGGTTCAGTGTGGACTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAg                                 <  2:4289977/93‑1 (MQ=255)

                                                         tatCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGc                                >  1:1215156/1‑93 (MQ=255)

                                                          atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                               <  1:4738739/93‑1 (MQ=255)

                                                          atCGGCCAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCa                               >  1:3582503/1‑93 (MQ=255)

                                                           tCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAg                              >  1:6453807/1‑93 (MQ=255)

                                                               ccAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcgg                          >  2:5289466/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  2:21138/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  1:4639795/1‑93 (MQ=255)

                                                                cAGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAggcggc                         >  1:2571308/1‑93 (MQ=255)

                                                                 aGGTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCt                        >  1:5081302/1‑93 (MQ=255)

                                                                  ggTTCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCtt                       >  1:4511512/1‑93 (MQ=255)

                                                                    ttCAGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCg                     >  1:7244625/1‑93 (MQ=255)

                                                                       aGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCac                  >  1:1621741/1‑92 (MQ=255)

                                                                       aGTGTGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCAt                  >  2:3677790/1‑93 (MQ=255)

                                                                        gtgtGGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCCTc                 >  1:6309003/1‑93 (MQ=255)

                                                                         tgtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                >  1:6795212/1‑93 (MQ=255)

                                                                         tgtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                >  2:4997957/1‑93 (MQ=255)

                                                                         tgtgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATcc                <  1:583016/93‑1 (MQ=255)

                                                                           tgGAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCg              >  2:4398075/1‑93 (MQ=255)

                                                                             gAGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCAGCCAGCGTCTGAGCAGGCGGCTTCGCATCCTGGt            >  1:6075542/1‑93 (MQ=255)

                                                                              aGTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTa           <  2:2007115/93‑1 (MQ=255)

                                                                               ggaTGGCCAGGCGCTGTTCGCGAGAGCGTCCCTTCCGTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTAt          <  1:1028670/91‑1 (MQ=255)

                                                                               gTATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGAATCCCGGTAt          >  1:1311232/1‑93 (MQ=255)

                                                                                tATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATc         <  2:5469058/93‑1 (MQ=255)

                                                                                tATGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATc         <  2:833082/93‑1 (MQ=255)

                                                                                 aTGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCa        <  1:7671959/93‑1 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       <  1:5861214/93‑1 (MQ=255)

                                                                                  tGGCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCaa       >  1:3370237/1‑93 (MQ=255)

                                                                                    gCCAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAg     <  2:4870882/93‑1 (MQ=255)

                                                                                     ccAGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgc    <  2:378370/93‑1 (MQ=255)

                                                                                       aGCCGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAgcgc  >  1:1916208/1‑93 (MQ=255)

CATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCTGCAACTGCCGCATATCGGCCAGGTTCAGTGTGGAGTATGGCCAGCTGCTGTTCGCGAGAGCGTCCCTTCTCTGCTGTAATAATTTATCGCCGCCAGCGTCTGAGCAGGCGGCTTCGCATCCCGGTATCAAAGCGC  >  NC_000913/3925652‑3925831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`