BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	442,916	A→G	V275A (GTA→GCA)	apbA ←	ApbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	442,916	0	A	G	98.6%	834.8 / ‑0.1	280	V275A (GTA→GCA)	apbA	ApbA
Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (142/134); total (144/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 301 total aligned reads displayed.

CACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAAC  >  NC_000913/442826‑443005

cACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCg                                                                                         <  2:4180244/93‑1 (MQ=255)

 aCTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACgg                                                                                        <  2:6430853/93‑1 (MQ=255)

   tACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCa                                                                                      >  1:3394681/1‑93 (MQ=255)

    aCCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCaa                                                                                     <  1:7087761/93‑1 (MQ=255)

    aCCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCaa                                                                                     <  2:6973070/93‑1 (MQ=255)

    aCCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCaa                                                                                     <  1:5451059/93‑1 (MQ=255)

       aGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATcc                                                                                  <  2:4692182/93‑1 (MQ=255)

        ggggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATccc                                                                                 >  1:6520650/1‑93 (MQ=255)

          ggCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCAt                                                                               <  2:3877044/93‑1 (MQ=255)

           gCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATg                                                                              <  2:6863956/93‑1 (MQ=255)

             gAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcg                                                                            <  1:379689/93‑1 (MQ=255)

             gAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcg                                                                            >  2:2431087/1‑93 (MQ=255)

             gAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcg                                                                            >  1:1295951/1‑93 (MQ=255)

              aGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcgc                                                                           >  2:6774789/1‑93 (MQ=255)

              aGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATgcgc                                                                           >  2:4347467/1‑93 (MQ=255)

               ggCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGGTTTCCCGTGCGGCAATCACACgcgcc                                                                          >  2:4445895/1‑92 (MQ=255)

                  aaaCCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCCGTGCGGCAATCCCATgcgcgccg                                                                       >  1:5051673/1‑91 (MQ=255)

                    aCCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggc                                                                     <  1:4944286/93‑1 (MQ=255)

                     ccAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcg                                                                    >  2:3056539/1‑93 (MQ=255)

                     ccAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcg                                                                    >  1:1838050/1‑93 (MQ=255)

                      cAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgc                                                                   >  1:6302061/1‑93 (MQ=255)

                        gTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgcgg                                                                 <  2:561895/93‑1 (MQ=255)

                        gTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCgcgcgggcgcgg                                                                 <  1:5705885/93‑1 (MQ=255)

                          gCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCgcggcg                                                               >  2:2787812/1‑93 (MQ=255)

                            cGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTa                                                             <  1:7440201/93‑1 (MQ=255)

                             gATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTaa                                                            <  1:831058/93‑1 (MQ=255)

                             gATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGGaa                                                            >  2:7244434/1‑93 (MQ=255)

                                gcgcTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAagag                                                         <  1:2960269/93‑1 (MQ=255)

                                  gcTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa                                                       <  1:3311369/93‑1 (MQ=255)

                                  gcTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaa                                                       >  1:5182815/1‑93 (MQ=255)

                                   cTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaaa                                                      <  2:770795/93‑1 (MQ=255)

                                   cTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGATTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaaa                                                      >  1:4908812/1‑93 (MQ=255)

                                    tCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGGGGAAGAGaaaa                                                     >  2:1533564/1‑93 (MQ=255)

                                    tCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGaaaa                                                     >  2:3199091/1‑93 (MQ=255)

                                     cATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAc                                                    >  2:5928169/1‑93 (MQ=255)

                                       tatTCGCTCTCCTTTCTTTTTACCATTGCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCa                                                  <  1:1336835/93‑1 (MQ=255)

                                       tatTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAccc                                                  >  1:2289630/1‑92 (MQ=255)

                                       tatTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCa                                                  <  2:5336670/93‑1 (MQ=255)

                                       tatTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCa                                                  <  2:5117103/93‑1 (MQ=255)

                                        atTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAt                                                 >  1:2143069/1‑93 (MQ=255)

                                        atTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAt                                                 >  1:3028852/1‑93 (MQ=255)

                                        atTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGGGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAAACAt                                                 >  2:5747887/1‑93 (MQ=255)

                                         ttCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCAtt                                                >  1:3627641/1‑93 (MQ=255)

                                           cACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGa                                              >  2:1353953/1‑93 (MQ=255)

                                           cACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGa                                              >  2:5405516/1‑93 (MQ=255)

                                            aCTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGat                                             >  2:367260/1‑93 (MQ=255)

                                             ctctCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata                                            >  2:5231202/1‑93 (MQ=255)

                                             ctctCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGata                                            <  1:6858143/93‑1 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         <  1:2603878/93‑1 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         <  2:5955613/93‑1 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         >  1:4864801/1‑93 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         >  1:5950658/1‑93 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         >  1:4588291/1‑93 (MQ=255)

                                                tcCTTTCTTTTTACCATTTCAAACAGGCGGGGGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAg                                         >  1:3077786/1‑93 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        >  1:3159535/1‑93 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        >  1:7715083/1‑93 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        >  1:5326634/1‑93 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        <  2:7285373/93‑1 (MQ=255)

                                                 ccTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGt                                        >  1:1076114/1‑93 (MQ=255)

                                                  ctttctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTc                                       >  1:4089244/1‑93 (MQ=255)

                                                  ctttctttTTACCATTTCAAACAGGCGGGTGTGTTCCGGTGCGGCAAGCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTc                                       <  2:2149947/93‑1 (MQ=255)

                                                  ctttctttTGACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTc                                       <  1:6216749/93‑1 (MQ=255)

                                                   tttctttTTACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCg                                      >  2:1721855/1‑93 (MQ=255)

                                                         tttACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCa                                >  2:7657606/1‑93 (MQ=255)

                                                          ttACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAg                               >  1:7851129/1‑93 (MQ=255)

                                                           tACCATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAgt                              <  2:3487657/93‑1 (MQ=255)

                                                              cATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCCTtgtg                           >  2:2366412/1‑93 (MQ=255)

                                                              cATTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGtgtg                           >  1:929915/1‑93 (MQ=255)

                                                               aTTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTgg                          >  2:1671456/1‑93 (MQ=255)

                                                               aTTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTgg                          >  1:2488356/1‑93 (MQ=255)

                                                               aTTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTgg                          >  2:2595812/1‑93 (MQ=255)

                                                               aTTTCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGGGTgg                          >  1:587880/1‑93 (MQ=255)

                                                                tttCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGgc                         >  2:4575571/1‑93 (MQ=255)

                                                                tttCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGgc                         >  1:7256100/1‑93 (MQ=255)

                                                                  tCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGgcgc                       >  2:6094386/1‑93 (MQ=255)

                                                                  tCAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGgcgc                       >  2:3794578/1‑93 (MQ=255)

                                                                   cAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCa                      <  1:3428684/93‑1 (MQ=255)

                                                                   cAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCa                      >  2:4835725/1‑93 (MQ=255)

                                                                   cAAACAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCa                      >  2:5041407/1‑93 (MQ=255)

                                                                   cAAACAGGCGGCTGTTTGCCGGTGCGGCAAGCCCATGCGCGCGCGCGCTGTGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCa                      <  1:1596744/93‑1 (MQ=255)

                                                                    aaaCAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAg                     <  2:5424051/93‑1 (MQ=255)

                                                                     aaCAGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgc                    >  2:704796/1‑93 (MQ=255)

                                                                        aGGCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgcgcg                 <  2:6529395/93‑1 (MQ=255)

                                                                         ggCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAgcgcgc                >  2:3291948/1‑93 (MQ=255)

                                                                          gCGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGcgcgcg               <  1:3696014/93‑1 (MQ=255)

                                                                           cGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCgg              >  2:6710785/1‑93 (MQ=255)

                                                                           cGGGTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCgg              >  1:4311276/1‑93 (MQ=255)

                                                                            gggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGa             >  2:5301116/1‑93 (MQ=255)

                                                                            gggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGa             <  1:1969838/93‑1 (MQ=255)

                                                                             ggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGat            >  2:4058467/1‑93 (MQ=255)

                                                                             ggTGTTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGat            >  1:3502936/1‑93 (MQ=255)

                                                                              gtgtTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGata           <  2:1464377/93‑1 (MQ=255)

                                                                              gtgtTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGata           <  2:6474646/93‑1 (MQ=255)

                                                                                gtTTTCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATc         <  2:3433244/93‑1 (MQ=255)

                                                                                 ttttCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATcc        >  1:2647483/1‑93 (MQ=255)

                                                                                  tttCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCt       <  1:1466576/93‑1 (MQ=255)

                                                                                  tttCCGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCt       <  2:4227343/93‑1 (MQ=255)

                                                                                      cGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCaa   >  1:4256580/1‑93 (MQ=255)

                                                                                      cGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCaa   <  1:3666869/93‑1 (MQ=255)

                                                                                      cGGTGCGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCaa   >  2:1968558/1‑93 (MQ=255)

CACTACCAGGGGCGAGGCAAACCAGTGCCGATGCGCTCATATTCACTCTCCTTTCTTTTTACCATTTCAAACAGGCGGGTGTTTTCCGGTACGGCAATCCCATGCGCGCGGGCGCGGCGTAAGAGAAAACCATTGATATAGTCGATTTCAGTGTGGCGCAGCGCGCGGATATCCTGCAAC  >  NC_000913/442826‑443005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`