BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,945,896	T→C	V63A (GTA→GCA)	yifE →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,945,896	0	T	C	99.6%	1431.6 / ‑3.9	458	V63A (GTA→GCA)	yifE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (229/227); total (230/228)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 480 total aligned reads displayed.

ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACA  >  NC_000913/3945804‑3945985

  aaCTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAt                                                                                         >  1:768838/1‑93 (MQ=255)

  aaCTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAt                                                                                         >  1:5012690/1‑93 (MQ=255)

  aaCTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTGGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAt                                                                                         <  1:2868841/93‑1 (MQ=255)

     tGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGcc                                                                                      <  2:1240205/93‑1 (MQ=255)

      gCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCg                                                                                     <  2:1081510/93‑1 (MQ=255)

       cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGt                                                                                    <  1:5664258/93‑1 (MQ=255)

       cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGt                                                                                    <  2:4079267/93‑1 (MQ=255)

        ttGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTg                                                                                   >  2:1134184/1‑93 (MQ=255)

        ttGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTg                                                                                   >  2:2178686/1‑93 (MQ=255)

         tGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTgg                                                                                  <  2:5979019/93‑1 (MQ=255)

         tGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTgg                                                                                  <  2:13433/93‑1 (MQ=255)

         tGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTgg                                                                                  <  2:6087577/93‑1 (MQ=255)

         tGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTgg                                                                                  >  2:7071278/1‑93 (MQ=255)

         tGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTgg                                                                                  <  1:1071053/93‑1 (MQ=255)

          gAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGc                                                                                 >  1:3073456/1‑93 (MQ=255)

          gAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGc                                                                                 <  2:7783643/93‑1 (MQ=255)

           aGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCg                                                                                >  1:5322957/1‑93 (MQ=255)

             cGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGaa                                                                              <  1:1662690/93‑1 (MQ=255)

              gTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAc                                                                             >  1:190099/1‑93 (MQ=255)

              gTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAc                                                                             >  1:7563441/1‑93 (MQ=255)

              gTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAc                                                                             >  1:776522/1‑93 (MQ=255)

               tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGCACg                                                                            >  1:607155/1‑93 (MQ=255)

               tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACg                                                                            >  1:3955135/1‑93 (MQ=255)

               tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACg                                                                            <  1:1234464/93‑1 (MQ=255)

               tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACg                                                                            >  1:5228416/1‑93 (MQ=255)

                 aTGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTg                                                                          >  2:7202026/1‑93 (MQ=255)

                  tGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGa                                                                         >  1:4079708/1‑93 (MQ=255)

                  tGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGa                                                                         >  2:6310856/1‑93 (MQ=255)

                  tGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGAGAACGTGa                                                                         >  2:331416/1‑93 (MQ=255)

                   ggTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAg                                                                        <  1:5442198/93‑1 (MQ=255)

                    gTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGc                                                                       >  1:4652639/1‑93 (MQ=255)

                      tATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCa                                                                     <  1:6540679/93‑1 (MQ=255)

                      tATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCa                                                                     >  1:2073268/1‑93 (MQ=255)

                       aTGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAg                                                                    <  1:3717916/93‑1 (MQ=255)

                         gCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTg                                                                  >  1:1853928/1‑93 (MQ=255)

                          ccTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGa                                                                 <  2:3864013/93‑1 (MQ=255)

                          ccTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGa                                                                 >  1:7128522/1‑93 (MQ=255)

                            ttCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACa                                                               >  2:5392293/1‑93 (MQ=255)

                            ttCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACa                                                               >  1:1326044/1‑93 (MQ=255)

                            ttCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACa                                                               >  2:6563816/1‑93 (MQ=255)

                             tCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAg                                                              >  1:2725218/1‑93 (MQ=255)

                               aaTGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGaa                                                            <  1:1557243/93‑1 (MQ=255)

                               aaTGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGaa                                                            >  2:5627982/1‑93 (MQ=255)

                                aTGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAg                                                           <  2:7721169/93‑1 (MQ=255)

                                   aGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAg                                                        <  2:7337491/93‑1 (MQ=255)

                                     ttGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGaa                                                      <  2:7425677/93‑1 (MQ=255)

                                     ttGGATCTTGACAACCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGaa                                                      <  2:5024387/93‑1 (MQ=255)

                                        gATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgc                                                   >  2:5753150/1‑93 (MQ=255)

                                        gATCTGGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgc                                                   <  1:801601/93‑1 (MQ=255)

                                         aTCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgcg                                                  <  1:6759319/93‑1 (MQ=255)

                                          tCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCgt                                                 >  2:4251359/1‑93 (MQ=255)

                                            ttGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCgtgt                                               <  1:4242542/93‑1 (MQ=255)

                                                cAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTc                                           <  2:7501259/93‑1 (MQ=255)

                                                cAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTc                                           >  2:3695579/1‑93 (MQ=255)

                                                cAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTc                                           <  1:3133069/93‑1 (MQ=255)

                                                 aaaCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTcc                                          >  1:2577000/1‑93 (MQ=255)

                                                 aaaCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGGGTGGGcc                                          >  2:1613590/1‑93 (MQ=255)

                                                    cgcgAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAg                                       <  2:5338084/93‑1 (MQ=255)

                                                    cgcgAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAg                                       <  2:2484859/93‑1 (MQ=255)

                                                       gAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtat                                    <  2:3428621/93‑1 (MQ=255)

                                                        gcccGTTTCCCGAGAAAGAGAACCTCTCGGTAGCAGCATGCCGGGGCGAACGTGACCCAGTGACAGAAGCAGACCGCGTGTGGTCCAAGtata                                   <  2:3149672/91‑1 (MQ=255)

                                                        aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtata                                   >  1:632352/1‑93 (MQ=255)

                                                        aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtata                                   <  2:2345933/93‑1 (MQ=255)

                                                         gCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTatat                                  >  2:2680649/1‑93 (MQ=255)

                                                           cGGTTCCCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGa                                <  2:5879694/93‑1 (MQ=255)

                                                            ggTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAc                               >  2:2919362/1‑93 (MQ=255)

                                                               tACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAcgcg                            >  1:5951183/1‑93 (MQ=255)

                                                                  cGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTAt                         <  1:5763577/93‑1 (MQ=255)

                                                                   gAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAACCAGAACGCGTGTGGTCCAAGTATATGACGCGTAtt                        >  1:2440904/1‑93 (MQ=255)

                                                                    aGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTa                       >  1:1008538/1‑93 (MQ=255)

                                                                    aGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTa                       >  1:6946526/1‑93 (MQ=255)

                                                                    aGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTa                       <  1:647107/93‑1 (MQ=255)

                                                                     ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      >  1:3231073/1‑93 (MQ=255)

                                                                     ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      <  2:45178/93‑1 (MQ=255)

                                                                     ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      <  1:3613756/93‑1 (MQ=255)

                                                                     ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      >  2:1438062/1‑93 (MQ=255)

                                                                     ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      >  1:4553954/1‑93 (MQ=255)

                                                                     ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      >  2:555447/1‑93 (MQ=255)

                                                                      gAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaac                     <  2:4792580/93‑2 (MQ=255)

                                                                      gAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAg                     <  2:6786583/93‑1 (MQ=255)

                                                                       aaGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGc                    <  1:1161748/93‑1 (MQ=255)

                                                                       aaGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGc                    <  1:3512829/93‑1 (MQ=255)

                                                                       aaGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGc                    <  1:4410998/93‑1 (MQ=255)

                                                                        agagAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCg                   <  2:6954474/93‑1 (MQ=255)

                                                                        agagAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCg                   <  2:1320567/93‑1 (MQ=255)

                                                                         gagaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGt                  >  2:2988623/1‑93 (MQ=255)

                                                                          agaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTc                 <  2:5261695/93‑1 (MQ=255)

                                                                          agaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTc                 <  2:2115236/93‑1 (MQ=255)

                                                                           gaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTcc                >  1:4363272/1‑93 (MQ=255)

                                                                           gaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTcc                >  1:972350/1‑93 (MQ=255)

                                                                               ctctTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCaaaa            >  1:3660356/1‑93 (MQ=255)

                                                                                 ctTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACg          <  1:4916361/93‑1 (MQ=255)

                                                                                  ttCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGt         <  2:836926/93‑1 (MQ=255)

                                                                                   tCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGtt        <  2:3036173/93‑1 (MQ=255)

                                                                                     gTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGtttt      <  1:5090428/93‑1 (MQ=255)

                                                                                     gTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGtttt      >  1:7509912/1‑93 (MQ=255)

                                                                                     gTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGtttt      <  1:2204805/93‑1 (MQ=255)

                                                                                      tAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTc     <  1:7409336/93‑1 (MQ=255)

                                                                                       agcagcATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTca    <  2:1859124/93‑1 (MQ=255)

                                                                                         cAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTcaca  <  2:446288/93‑1 (MQ=255)

ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACA  >  NC_000913/3945804‑3945985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`