BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,974,008	C→T	A40V (GCG→GTG)	wzxE →	putative cytochrome

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,974,008	0	C	T	99.1%	1153.6 / ‑4.8	340	A40V (GCG→GTG)	wzxE	putative cytochrome
Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (160/176); total (164/176)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

CGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACC  >  NC_000913/3973918‑3974097

cggcggCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCtggtgg                                                                                         >  1:198248/1‑93 (MQ=255)

      ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAAtt                                                                                   >  2:1052307/1‑93 (MQ=255)

      ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAAtt                                                                                   >  2:6808851/1‑93 (MQ=255)

      ccAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAAtt                                                                                   <  1:3163440/93‑1 (MQ=255)

        ggTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTc                                                                                 >  2:5879343/2‑93 (MQ=255)

         gTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTcc                                                                                >  1:7822980/1‑93 (MQ=255)

           acacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGc                                                                              >  1:5109920/1‑93 (MQ=255)

           acacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGc                                                                              >  1:3371678/1‑93 (MQ=255)

            cacTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGcc                                                                             <  1:1120830/93‑1 (MQ=255)

             acTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCa                                                                            <  1:233807/93‑1 (MQ=255)

             acTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCa                                                                            <  1:40819/93‑1 (MQ=255)

               tGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGt                                                                          <  1:2680073/93‑1 (MQ=255)

               tGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGGGTCAAATTTCTGCCAGt                                                                          >  2:7023409/1‑93 (MQ=255)

                ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGtt                                                                         <  1:6203835/93‑1 (MQ=255)

                ggTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGtt                                                                         <  2:1897168/93‑1 (MQ=255)

                 gTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTg                                                                        <  1:5056437/93‑1 (MQ=255)

                 gTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTg                                                                        >  2:2081913/1‑93 (MQ=255)

                  tCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGa                                                                       >  2:7909557/1‑93 (MQ=255)

                  tCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGa                                                                       <  2:3119500/93‑1 (MQ=255)

                   cAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGAt                                                                      >  2:7541476/1‑93 (MQ=255)

                    aaGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGAtt                                                                     >  2:3872431/1‑93 (MQ=255)

                    aaGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGAtt                                                                     <  1:3289263/93‑1 (MQ=255)

                    aaGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGAtt                                                                     >  2:2848358/1‑93 (MQ=255)

                     aGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTa                                                                    >  1:5813830/1‑93 (MQ=255)

                      gATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTAc                                                                   >  2:178394/1‑93 (MQ=255)

                       aTTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTAcc                                                                  <  1:4297991/93‑1 (MQ=255)

                       aTTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTAcc                                                                  >  2:7708640/1‑93 (MQ=255)

                       aTTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTAcc                                                                  <  2:3599824/93‑1 (MQ=255)

                         tGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGt                                                                >  2:6066235/1‑93 (MQ=255)

                            tGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCt                                                             <  1:6126173/93‑1 (MQ=255)

                             gCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTc                                                            >  1:537577/1‑93 (MQ=255)

                              ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTTGCAAATTTCCGCCAGTTGATTACCGTGCTCg                                                           >  2:5535308/1‑93 (MQ=255)

                              ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCg                                                           <  2:7470607/93‑1 (MQ=255)

                              ccGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCg                                                           <  2:851689/93‑1 (MQ=255)

                               cGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCgg                                                          >  2:5477970/1‑93 (MQ=255)

                                gggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGc                                                         <  2:4218137/93‑1 (MQ=255)

                                gggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGc                                                         <  1:7141770/93‑1 (MQ=255)

                                 ggTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCg                                                        <  1:6809021/93‑1 (MQ=255)

                                   ttACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTg                                                      >  2:4719662/1‑93 (MQ=255)

                                    tACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGc                                                     <  2:4567509/93‑1 (MQ=255)

                                    tACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGc                                                     <  1:7565471/93‑1 (MQ=255)

                                     aCTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCt                                                    >  2:6435970/1‑93 (MQ=255)

                                      cTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCtt                                                   >  1:4585265/1‑93 (MQ=255)

                                       tGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCttc                                                  >  1:5491949/1‑92 (MQ=255)

                                       tGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTg                                                  >  2:4861118/1‑93 (MQ=255)

                                       tGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTg                                                  >  1:5301558/1‑93 (MQ=255)

                                        ggTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGc                                                 >  2:4124842/1‑93 (MQ=255)

                                          tCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCg                                               <  1:7395006/93‑1 (MQ=255)

                                           cGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCgg                                              >  1:2462069/1‑93 (MQ=255)

                                           cGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCgg                                              <  1:3560966/93‑1 (MQ=255)

                                            ggTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCggg                                             <  1:1206913/93‑1 (MQ=255)

                                              tAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                           <  1:6260307/93‑1 (MQ=255)

                                              tAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                           <  1:4276820/93‑1 (MQ=255)

                                              tAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                           >  1:5933482/1‑93 (MQ=255)

                                              tAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGc                                           <  2:7394426/93‑1 (MQ=255)

                                               taGTTGCTGGCTGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                          <  1:6047770/92‑1 (MQ=255)

                                               aaGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCt                                          <  2:6174490/93‑1 (MQ=255)

                                               aaGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCATGCTCGGCGTGCTTGCCGGGGCt                                          >  2:5379188/1‑93 (MQ=255)

                                                aGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTg                                         <  2:6499950/93‑1 (MQ=255)

                                                aGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTg                                         <  2:4354365/93‑1 (MQ=255)

                                                aGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTg                                         >  1:653209/1‑93 (MQ=255)

                                                 gTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTTGGCTGGTGGTAAATTTCCGCCAGTTTATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                        >  2:1693445/1‑93 (MQ=255)

                                                 gTTGCTGGCGCTGTCATTTGGTCCGGCGGGGCTGGAGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTgg                                        <  1:2860393/93‑1 (MQ=255)

                                                  ttGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGc                                       <  2:154334/93‑1 (MQ=255)

                                                  ttGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGc                                       <  2:1937447/93‑1 (MQ=255)

                                                      tGGCGGTGTCATTTGGGCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                   <  1:1809603/93‑1 (MQ=255)

                                                      cgggggTGTCATTTGGGCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCt                                   <  1:1561100/89‑1 (MQ=255)

                                                         cGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTa                                <  2:3258748/93‑1 (MQ=255)

                                                         cGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTa                                <  1:4427565/93‑1 (MQ=255)

                                                          ggTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                               >  2:1196813/1‑93 (MQ=255)

                                                          ggTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTaa                               <  1:895989/93‑1 (MQ=255)

                                                          ggTGTCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCATGCTTGCCGGGGCTGGCATCTTTaa                               <  2:5519400/93‑1 (MQ=255)

                                                           gtgtTATGTGGTCCGGCGGGCCTTGGGCTGTTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAAc                              <  2:1312273/93‑1 (MQ=255)

                                                           gtgtCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAAc                              >  1:4106374/1‑93 (MQ=255)

                                                             gtCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                            >  2:7282071/1‑93 (MQ=255)

                                                             gtCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACgg                            >  1:370095/1‑93 (MQ=255)

                                                              tCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                           >  2:7216644/1‑93 (MQ=255)

                                                              tCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                           <  2:6708612/93‑1 (MQ=255)

                                                              tCATTTGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGgt                           <  2:5750187/93‑1 (MQ=255)

                                                                 tttGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTa                        >  1:5405199/1‑93 (MQ=255)

                                                                  ttGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTaa                       <  1:2545238/93‑1 (MQ=255)

                                                                  ttGGTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTCATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTaa                       <  1:2059502/93‑1 (MQ=255)

                                                                     gTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                    >  1:7752580/1‑93 (MQ=255)

                                                                     gTCCGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCa                    >  1:3121063/1‑93 (MQ=255)

                                                                       ccGGCGGGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCaaa                  <  2:6304043/93‑1 (MQ=255)

                                                                        ctgcggGGCTTGGGCGGCTTGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAAt                 <  2:2712419/91‑1 (MQ=255)

                                                                         ggcggGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                <  1:5647262/93‑1 (MQ=255)

                                                                         ggcggGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATa                <  1:7498806/93‑1 (MQ=255)

                                                                          gcggGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATAc               >  1:7226195/1‑93 (MQ=255)

                                                                           cggGGCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACg              <  2:5079484/93‑1 (MQ=255)

                                                                            ggggCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGt             <  1:6392701/93‑1 (MQ=255)

                                                                            ggggCTTGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGt             >  1:4809902/1‑93 (MQ=255)

                                                                                 ttGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc        >  1:3756108/1‑93 (MQ=255)

                                                                                 ttGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc        >  1:4212727/1‑93 (MQ=255)

                                                                                 ttGGGCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc        <  1:4640589/93‑1 (MQ=255)

                                                                                 ttGGGCTGGAGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGccc        >  2:2440049/1‑93 (MQ=255)

                                                                                   gggCTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTGACCAAATACGTTGCCCAg      >  1:2948096/1‑93 (MQ=255)

                                                                                      cTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTAc   >  1:7066370/1‑93 (MQ=255)

                                                                                      cTGGTGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTAc   <  1:2310927/93‑1 (MQ=255)

                                                                                       tggtggCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTAcc  >  2:5252264/1‑93 (MQ=255)

CGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGTTGCTGGCGGTGTCATTTGGTCCGGCGGGGCTTGGGCTGGCGGCAAATTTCCGCCAGTTGATTACCGTGCTCGGCGTGCTTGCCGGGGCTGGCATCTTTAACGGTGTAACCAAATACGTTGCCCAGTACC  >  NC_000913/3973918‑3974097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`