BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,346	A→G	V475A (GTG→GCG)	ampG ←	AmpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,346	0	A	G	99.1%	704.6 / ‑3.4	230	V475A (GTG→GCG)	ampG	AmpG
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (141/87); total (143/87)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 244 total aligned reads displayed.

ACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAAT  >  NC_000913/451256‑451435

 cGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCgcga                                                                                        <  1:6243777/93‑1 (MQ=255)

 cGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCgcga                                                                                        <  2:5308286/93‑1 (MQ=255)

 aggCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTGCGTAGCGCCAGATAATCCAGCAAACCACCAAGCgcga                                                                                        <  1:7867409/92‑1 (MQ=255)

     gAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGcc                                                                                    >  1:5245471/1‑93 (MQ=255)

        tGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGa                                                                                 >  2:2701460/1‑93 (MQ=255)

        tGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGa                                                                                 >  1:5486413/1‑93 (MQ=255)

         gATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGaa                                                                                >  1:6483235/1‑93 (MQ=255)

         gATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGaa                                                                                <  2:7392716/93‑1 (MQ=255)

          aTTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGaaa                                                                               >  1:4467201/1‑93 (MQ=255)

          aTTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGaaa                                                                               <  1:4149018/93‑1 (MQ=255)

          aTTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGaaa                                                                               >  2:598881/1‑93 (MQ=255)

          aTTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGaaa                                                                               <  1:5837360/93‑1 (MQ=255)

           ttACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAg                                                                              >  2:7473335/1‑93 (MQ=255)

            tactacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAgg                                                                             >  1:7653290/1‑93 (MQ=255)

            tactacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAgg                                                                             >  2:179715/1‑93 (MQ=255)

              ctacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGc                                                                           >  1:4779388/1‑93 (MQ=255)

              ctacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGc                                                                           >  1:6163676/1‑93 (MQ=255)

              ctacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGc                                                                           >  2:407082/1‑93 (MQ=255)

               tacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCg                                                                          >  1:7605910/1‑93 (MQ=255)

               tacAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCg                                                                          >  1:7118697/1‑93 (MQ=255)

                acAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGa                                                                         >  2:2508298/1‑93 (MQ=255)

                 cAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGAc                                                                        <  1:1895989/93‑1 (MQ=255)

                   gCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAACGCGACGCCAGAAAGGGCGACTa                                                                      >  1:2648109/1‑93 (MQ=255)

                     tAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTaaa                                                                    >  2:7827495/1‑93 (MQ=255)

                      aaaTAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTaaaa                                                                   <  2:5457587/93‑1 (MQ=255)

                       aataatATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAc                                                                  >  2:6151750/1‑93 (MQ=255)

                       aataatATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAc                                                                  >  2:6268669/1‑93 (MQ=255)

                        ataataTTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAcc                                                                 >  1:4907132/1‑93 (MQ=255)

                        ataataTTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAcc                                                                 <  1:5170320/93‑1 (MQ=255)

                         taataTTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAccc                                                                >  1:3480168/1‑93 (MQ=255)

                         taataTTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAccc                                                                >  2:7183281/1‑93 (MQ=255)

                          aataTTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATCATCCAGCAAACCATCAAGCTCGACGCCAGAAACGGCGCCTCAAAcccc                                                               >  1:6321219/1‑93 (MQ=255)

                          aataTTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAAcccc                                                               <  2:1032783/93‑1 (MQ=255)

                           atatTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCg                                                              <  2:2829471/93‑1 (MQ=255)

                           atatTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCg                                                              >  1:891872/1‑93 (MQ=255)

                           atatTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCg                                                              >  1:4196517/1‑93 (MQ=255)

                            tatTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGa                                                             >  2:6899061/1‑93 (MQ=255)

                              tttACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACt                                                           >  2:1144547/1‑93 (MQ=255)

                               ttACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACtt                                                          <  2:7646601/93‑1 (MQ=255)

                                tACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTc                                                         >  2:3275549/1‑93 (MQ=255)

                                tACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTc                                                         >  1:2030202/1‑93 (MQ=255)

                                tACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTc                                                         >  1:1142665/1‑93 (MQ=255)

                                   aGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcag                                                      >  2:7377835/1‑93 (MQ=255)

                                    gATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcagc                                                     <  2:1354970/93‑1 (MQ=255)

                                     aTTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcagca                                                    >  2:2020085/1‑93 (MQ=255)

                                     aTTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcagca                                                    >  2:1424092/1‑93 (MQ=255)

                                     aTTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcagca                                                    >  2:3330360/1‑93 (MQ=255)

                                      ttACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcagcag                                                   <  2:7721413/93‑1 (MQ=255)

                                      ttACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCcagcag                                                   >  1:7264766/1‑93 (MQ=255)

                                       tACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCagcaga                                                  >  1:3626379/1‑93 (MQ=255)

                                        aCGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCagcagag                                                 >  1:2517402/1‑93 (MQ=255)

                                          gTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCagcagagca                                               <  2:7778595/93‑1 (MQ=255)

                                           tCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGTCTAAAACCCCGACTTCCagcagagcag                                              >  2:2070290/1‑93 (MQ=255)

                                           tCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCagcagagcag                                              >  1:5902887/1‑93 (MQ=255)

                                           tCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCagcagagcag                                              <  1:3232094/93‑1 (MQ=255)

                                            cAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcagg                                             >  1:2818095/1‑93 (MQ=255)

                                            cAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGAGCGACTACAACCCCGACTTCCAGCAGAgccgg                                             >  1:4266755/1‑93 (MQ=255)

                                             aGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggc                                            >  2:4995884/1‑93 (MQ=255)

                                             aGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggc                                            >  2:428794/1‑93 (MQ=255)

                                             aGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggc                                            >  1:4506172/1‑93 (MQ=255)

                                             aGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggc                                            >  2:393184/1‑93 (MQ=255)

                                              gATGCGTTTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggc                                            >  2:3719355/1‑93 (MQ=255)

                                              gATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggca                                           >  1:6621496/1‑93 (MQ=255)

                                              gATGCG‑TTTTTCGTAGCGCCAGATAATCAAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAgcaggca                                           <  1:6757482/93‑1 (MQ=255)

                                                 gCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGa                                        <  1:6021574/93‑1 (MQ=255)

                                                   g‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGaaa                                      <  1:1050952/93‑1 (MQ=255)

                                                   g‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGaaa                                      <  1:4294669/93‑1 (MQ=255)

                                                        ttCGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAgaga                                  <  1:3104562/93‑1 (MQ=255)

                                                          cGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAgagagg                                >  2:6602211/1‑91 (MQ=255)

                                                          cGTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAgagagg                                <  2:2580933/93‑3 (MQ=255)

                                                           gTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAgagaggg                               >  2:2191335/1‑90 (MQ=255)

                                                           gTAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGt                               <  1:1458122/93‑1 (MQ=255)

                                                            tAGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTg                              <  2:5279486/93‑1 (MQ=255)

                                                             aGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGc                             <  1:3958179/93‑1 (MQ=255)

                                                             aGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGc                             <  2:1275449/93‑1 (MQ=255)

                                                             aGCGCCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGc                             >  1:6911139/1‑93 (MQ=255)

                                                              gcgcCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCg                            <  1:5451102/93‑1 (MQ=255)

                                                                gcCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTc                          <  1:1244991/93‑1 (MQ=255)

                                                                gcCAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGCGCGTc                          >  2:1060864/1‑93 (MQ=255)

                                                                 ccAGATAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCa                         <  2:3109168/93‑1 (MQ=255)

                                                                     aTAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATc                     <  2:3731509/93‑1 (MQ=255)

                                                                     aTAATCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATc                     >  2:1509814/1‑93 (MQ=255)

                                                                        aTCCAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAg                  <  1:467977/93‑1 (MQ=255)

                                                                          ccAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAgcg                >  1:7005005/1‑93 (MQ=255)

                                                                           cAGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAgcgc               <  1:2684000/93‑1 (MQ=255)

                                                                            aGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGcgcg              >  2:6297142/1‑93 (MQ=255)

                                                                            aGCAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGcgcg              >  2:4436161/1‑93 (MQ=255)

                                                                              cAAACCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCAACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTc            <  2:4789088/93‑1 (MQ=255)

                                                                               aaaCCACCAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCACATCCAGCGCGTcc           >  2:5866541/1‑93 (MQ=255)

                                                                                 accaccAAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCAt         >  1:4627997/1‑93 (MQ=255)

                                                                                    accaAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGt      >  2:524711/1‑93 (MQ=255)

                                                                                    accaAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGt      >  2:2434036/1‑93 (MQ=255)

                                                                                     ccaAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTc     >  2:7923302/1‑93 (MQ=255)

                                                                                      caAGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCa    >  2:836536/1‑93 (MQ=255)

                                                                                      aaaGCGCCACGCCAGAAAGGGCGAAAAAAGCAACACCTCCAAGCAGAGCAGGCAAGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCa    <  2:6995538/92‑1 (MQ=255)

                                                                                       aaGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCaa   <  1:2778275/93‑1 (MQ=255)

                                                                                       aaGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCaa   <  1:1553021/93‑1 (MQ=255)

                                                                                       aaGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCaa   <  1:5102998/93‑1 (MQ=255)

                                                                                        aGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCGGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAAt  <  2:4922222/93‑1 (MQ=255)

                                                                                        aGCGCGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAGGTGCGTCAAATCCAGCGCGTCCATCGTCAAt  <  1:6070767/93‑1 (MQ=255)

ACGGCGAGTGATTACTACAGCTAAATAATATTTACAGATTACGTCAGATGCG‑TTTTTCGTAGCGCCAGATAATCCAGCAAACCACCAAGCACGACGCCAGAAAGGGCGACTAAAACCCCGACTTCCAGCAGAGCAGGCAGGAAAGAGAAGTGCGTCAAATCCAGCGCGTCCATCGTCAAT  >  NC_000913/451256‑451435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`