Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,477 T→C Y456H (TAC→CAC)  lon → ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,4770TC99.3% 951.4 / ‑3.1 303Y456H (TAC→CAC) lonATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (168/133);  minor base A (2/0);  total (170/133)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-02

Only 100 of 337 total aligned reads displayed.

GACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATG  >  NC_000913/459387‑459566
                                                                                          |                                                                                         
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      >  1:5147129/1‑93 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      >  1:5323361/1‑93 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      <  1:6167918/93‑1 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      <  1:7855034/93‑1 (MQ=255)
   aaaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTg                                                                                      <  1:923339/93‑1 (MQ=255)
    aaaTGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTgg                                                                                     >  2:5080260/1‑93 (MQ=255)
       tGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAg                                                                                  <  2:3758493/93‑1 (MQ=255)
       tGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAg                                                                                  >  1:5238949/1‑93 (MQ=255)
        gTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGt                                                                                 >  1:2255003/1‑93 (MQ=255)
         tcttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTg                                                                                >  1:4624637/1‑93 (MQ=255)
          cttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTgg                                                                               <  1:3093741/93‑1 (MQ=255)
           ttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCGGCGACCACCACCTGGAAGTGGa                                                                              >  1:2669946/1‑93 (MQ=255)
           ttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGa                                                                              >  1:5201613/1‑93 (MQ=255)
           ttctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGa                                                                              >  2:4805549/1‑93 (MQ=255)
            tctGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGAt                                                                             >  2:117137/1‑93 (MQ=255)
              tGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTa                                                                           >  2:1881113/1‑93 (MQ=255)
              tGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTa                                                                           >  2:3284118/1‑93 (MQ=255)
               gtcATTCGTGGCGATCCTGCCTCTGCACTGCTTGAAGTGCTGGATCCAGATCAGAACGTAGCGTTCAGCGACCTCCACCTGGAAGTGGATTAc                                                                          >  2:7028109/3‑93 (MQ=255)
               gACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTAc                                                                          <  2:6223383/93‑1 (MQ=255)
               gACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTAc                                                                          >  1:6962241/1‑93 (MQ=255)
               gACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTAc                                                                          <  1:4868685/93‑1 (MQ=255)
                 cATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGa                                                                        <  1:1150062/93‑1 (MQ=255)
                  aTGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAt                                                                       >  1:1847116/1‑93 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGATGTGGATTACGaac                                                                      >  2:495621/1‑91 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  2:6326911/1‑93 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  1:549580/1‑93 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      <  1:5413995/93‑1 (MQ=255)
                   tGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtc                                                                      >  2:2191624/1‑93 (MQ=255)
                    gCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGAtct                                                                     >  1:355938/1‑93 (MQ=255)
                      gTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAGCGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCa                                                                   <  2:6037949/93‑1 (MQ=255)
                       tGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAg                                                                  >  1:3787321/1‑93 (MQ=255)
                        ggCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGc                                                                 >  2:1569033/1‑93 (MQ=255)
                         gCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCg                                                                >  1:509730/1‑93 (MQ=255)
                         gCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCg                                                                >  1:6763006/1‑93 (MQ=255)
                          cGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCGGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGa                                                               >  2:1228065/1‑93 (MQ=255)
                          cGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGa                                                               <  1:142953/93‑1 (MQ=255)
                          cGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGa                                                               <  2:3998729/93‑1 (MQ=255)
                           gATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGAc                                                              >  1:7688894/1‑93 (MQ=255)
                           gATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGAc                                                              >  2:526097/1‑93 (MQ=255)
                            aTCCGGCCTCTGCACTGCTTGGAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACg                                                             >  2:38845/1‑93 (MQ=255)
                            aTCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACg                                                             >  2:3148208/1‑93 (MQ=255)
                             tCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGt                                                            <  1:3009653/93‑1 (MQ=255)
                               cGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGa                                                          <  2:6419165/93‑1 (MQ=255)
                                 gCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATg                                                        <  1:4557135/93‑1 (MQ=255)
                                 gCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATg                                                        >  1:2504836/1‑93 (MQ=255)
                                   ctctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGtt                                                      <  2:7681823/93‑1 (MQ=255)
                                    tctGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt                                                     <  1:5700201/93‑1 (MQ=255)
                                      tGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGt                                                   <  1:3325976/93‑1 (MQ=255)
                                       gCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTc                                                  <  1:4408547/93‑1 (MQ=255)
                                         aCTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcgc                                                >  1:6274545/1‑93 (MQ=255)
                                         aCTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcgc                                                <  1:7363254/93‑1 (MQ=255)
                                           tGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTcgcgc                                              >  2:5636380/1‑92 (MQ=255)
                                           tGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa                                              >  1:3243910/1‑93 (MQ=255)
                                           tGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa                                              <  1:3418571/93‑1 (MQ=255)
                                           tGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGa                                              >  2:422647/1‑93 (MQ=255)
                                            gCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc                                             >  1:6087540/1‑93 (MQ=255)
                                            gCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGAc                                             <  1:4451073/93‑1 (MQ=255)
                                              ttGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGt                                           <  2:498878/93‑1 (MQ=255)
                                                gAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCg                                         >  1:3487255/1‑93 (MQ=255)
                                                gAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCg                                         >  1:6539955/1‑93 (MQ=255)
                                                  aGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGaa                                       <  2:6955023/93‑1 (MQ=255)
                                                    tGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACt                                     <  1:5774206/93‑1 (MQ=255)
                                                     gCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTc                                    <  1:593704/93‑1 (MQ=255)
                                                      cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt                                                     <  1:4115237/75‑1 (MQ=255)
                                                      cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt                                                     >  2:4115236/1‑75 (MQ=255)
                                                      cTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTcc                                   >  2:1991713/1‑93 (MQ=255)
                                                       tGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCa                                  >  2:1048989/1‑93 (MQ=255)
                                                        ggATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCAt                                 <  1:2383510/93‑1 (MQ=255)
                                                        ggATCCAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCAt                                 >  2:3693596/1‑93 (MQ=255)
                                                           tCCAGAGCAGAACGTAGCATTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGaa                              >  2:2259470/1‑93 (MQ=255)
                                                            ccAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAAc                             >  1:3624852/1‑93 (MQ=255)
                                                            ccAGAGCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAAc                             <  1:7341573/93‑1 (MQ=255)
                                                              agagCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAt                           <  1:2867185/93‑1 (MQ=255)
                                                               gagCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACAtt                          >  2:2033626/1‑93 (MQ=255)
                                                                agCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTc                         <  2:6155738/93‑1 (MQ=255)
                                                                agCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTc                         >  1:421383/1‑93 (MQ=255)
                                                                 gCAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTcc                        <  2:5445137/93‑1 (MQ=255)
                                                                  cAGAACGTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCg                       <  2:2886908/93‑1 (MQ=255)
                                                                        gTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCg                 <  1:1068367/93‑1 (MQ=255)
                                                                        gTAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCg                 >  2:1635961/1‑93 (MQ=255)
                                                                         tAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgc                >  1:7774512/1‑93 (MQ=255)
                                                                         tAGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgc                >  1:7121235/1‑93 (MQ=255)
                                                                          aGCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgct               <  1:6656806/93‑1 (MQ=255)
                                                                           gCGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgctg              <  2:7822996/93‑1 (MQ=255)
                                                                            cGTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgctgc             <  2:754180/93‑1 (MQ=255)
                                                                             gTTCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCgctgct            >  1:1697200/1‑93 (MQ=255)
                                                                              ttCAGCGACCCCCTCCTGGAAGTGGATTACGATCTCAGCGACGGGATGTTTTTCGCGACGTCGAACTCCATGAACATTCCGGCACCGctgctg           <  2:2594485/93‑1 (MQ=255)
                                                                              ttCAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGctgctg           >  2:5865545/1‑93 (MQ=255)
                                                                                cAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGa         >  1:885095/1‑93 (MQ=255)
                                                                                cAGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGa         <  2:775773/93‑1 (MQ=255)
                                                                                cAGCGACCACAACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGa         >  2:4417848/1‑93 (MQ=255)
                                                                                 aGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGAt        <  2:2463691/93‑1 (MQ=255)
                                                                                 aGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGAt        <  2:1480750/93‑1 (MQ=255)
                                                                                 aGCGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTCGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGAt        <  2:6720028/93‑1 (MQ=255)
                                                                                   cGACCACCACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCg      <  1:2758826/93‑1 (MQ=255)
                                                                                     cccaccaCCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTa    <  2:7893541/92‑1 (MQ=255)
                                                                                      ccaccaCCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTAt   <  2:3453250/93‑1 (MQ=255)
                                                                                      ccaccaCCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTAt   <  1:153831/93‑1 (MQ=255)
                                                                                      ccaccaCCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTAt   <  2:7195286/93‑1 (MQ=255)
                                                                                       caccacCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATg  >  1:4336224/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATG  >  NC_000913/459387‑459566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑