BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,176	C→G	G182R (GGA→CGA)	ysgA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,176	0	C	G	99.5%	1177.8 / ‑5.6	373	G182R (GGA→CGA)	ysgA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (200/171); minor base T (2/0); total (202/171)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 380 total aligned reads displayed.

TTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCCG‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAC  >  NC_000913/4013092‑4013260

ttAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTa                                                                               >  1:6829441/1‑93 (MQ=255)

 tAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTaa                                                                              <  2:3558225/93‑1 (MQ=255)

  agcagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAg                                                                             <  2:901117/93‑1 (MQ=255)

   gcagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGa                                                                            <  1:2133849/93‑1 (MQ=255)

    cagcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGAt                                                                           <  2:6442828/93‑1 (MQ=255)

      gcCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCg                                                                         >  2:2960556/1‑93 (MQ=255)

       cccGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCgg                                                                        <  2:2048125/93‑1 (MQ=255)

       cccGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCgg                                                                        >  1:3679840/1‑93 (MQ=255)

        ccGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGt                                                                       <  1:7099891/93‑1 (MQ=255)

        ccGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGt                                                                       <  1:4240038/93‑1 (MQ=255)

         cGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGtt                                                                      >  1:6257432/1‑93 (MQ=255)

          gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTg                                                                     <  1:518557/93‑1 (MQ=255)

          gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTg                                                                     >  2:1976857/1‑93 (MQ=255)

          gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTg                                                                     >  1:6628800/1‑93 (MQ=255)

          gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTg                                                                     <  2:5262587/93‑1 (MQ=255)

          gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTg                                                                     >  1:3980119/1‑93 (MQ=255)

          gCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGACGAGA‑ATCGGCGCGTTAAGATCGGTTg                                                                     >  1:4632167/1‑93 (MQ=255)

           cAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGc                                                                    <  1:7321451/93‑1 (MQ=255)

            aGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCg                                                                   >  2:1741523/1‑93 (MQ=255)

             gcgcCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGa                                                                  <  2:1369667/93‑1 (MQ=255)

               gcCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCTGCGCGTTAAGATCGGTTGCGata                                                                >  2:4295432/1‑93 (MQ=255)

                ccTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGatat                                                               <  1:144016/93‑1 (MQ=255)

                ccTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGatat                                                               >  2:6021867/1‑93 (MQ=255)

                 cTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATc                                                              <  1:4121452/93‑1 (MQ=255)

                 cTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATc                                                              >  2:7767272/1‑93 (MQ=255)

                   ggCGCATGGTTTCCACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCaa                                                            >  1:6375401/1‑93 (MQ=255)

                   ggCGCATGGTTTCATCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCaa                                                            <  2:5155201/93‑1 (MQ=255)

                    gcgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAAc                                                           <  2:2692481/93‑1 (MQ=255)

                    gcgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAAc                                                           <  2:3301747/93‑1 (MQ=255)

                     cgcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACa                                                          <  2:866712/93‑1 (MQ=255)

                      gcATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAg                                                         >  1:4213618/1‑93 (MQ=255)

                        aTGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgt                                                       <  1:2453738/93‑1 (MQ=255)

                        aTGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgt                                                       <  2:593138/93‑1 (MQ=255)

                        aTGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgt                                                       <  2:4884775/93‑1 (MQ=255)

                        aTGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGGTGCGATATCAACAGgt                                                       >  1:5360184/1‑93 (MQ=255)

                          ggTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttg                                                     >  1:44635/1‑93 (MQ=255)

                           gTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgt                                                    >  1:1060659/1‑93 (MQ=255)

                           gTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGgttgt                                                    <  2:2795118/93‑1 (MQ=255)

                             ttCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGttt                                                  <  2:625622/93‑1 (MQ=255)

                              tCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTc                                                 <  2:2419419/93‑1 (MQ=255)

                               cAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCg                                                <  2:7457041/93‑1 (MQ=255)

                                aaCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCgg                                               <  1:841216/93‑1 (MQ=255)

                                 aCGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGt                                              <  1:5215308/93‑1 (MQ=255)

                                    ctctCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGaa                                           <  2:4038930/93‑1 (MQ=255)

                                    ctctCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATAACAACAGGTTGTTTCGGTGaa                                           <  1:5845942/93‑1 (MQ=255)

                                     tctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAAt                                          >  1:6095905/1‑93 (MQ=255)

                                     tctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAAt                                          >  1:3510758/1‑93 (MQ=255)

                                      ctcCTGCGGTATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAAtt                                         <  1:1863825/93‑1 (MQ=255)

                                      ctcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAAtt                                         >  1:1275327/1‑93 (MQ=255)

                                       tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTc                                        >  2:5638257/1‑93 (MQ=255)

                                       tcCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTc                                        <  2:707631/93‑1 (MQ=255)

                                        ccTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCa                                       >  1:74959/1‑93 (MQ=255)

                                         cTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAg                                      <  2:4597509/93‑1 (MQ=255)

                                          tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                                     >  2:5440730/1‑93 (MQ=255)

                                          tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                                     >  1:4786516/1‑93 (MQ=255)

                                          tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                                     >  2:3453986/1‑93 (MQ=255)

                                          tGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGc                                     <  2:3263905/93‑1 (MQ=255)

                                           gCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCg                                    >  1:201163/1‑93 (MQ=255)

                                           gCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCg                                    >  2:7291707/1‑93 (MQ=255)

                                            cGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGa                                   <  2:6734951/93‑1 (MQ=255)

                                              gAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCAACt                                 <  1:88060/93‑1 (MQ=255)

                                               aaTGCGGTTATTCTTTCCACCATATATTTCGAGATTTGTT‑GCGGTAAGATCGGTTGCGATTTCAACAGGTTGTTTCTGTTTTTTCAGCTACtg                                >  1:5998719/1‑92 (MQ=255)

                                                aTGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTg                               >  1:2347848/1‑93 (MQ=255)

                                                 tGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGt                              <  1:5687507/93‑1 (MQ=255)

                                                 agCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGt                              <  2:6648544/92‑1 (MQ=255)

                                                  gCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTc                             <  1:3150274/93‑1 (MQ=255)

                                                  gCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTc                             >  1:1689880/1‑93 (MQ=255)

                                                    tGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGc                           >  2:3835225/1‑93 (MQ=255)

                                                    tGTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGc                           >  2:4756711/1‑93 (MQ=255)

                                                     gTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGcc                          >  1:2821834/1‑93 (MQ=255)

                                                     gTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGcc                          <  1:5792119/93‑1 (MQ=255)

                                                     gTTATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGcc                          >  1:775758/1‑93 (MQ=255)

                                                      ttATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGccc                         >  2:7696865/1‑93 (MQ=255)

                                                       tATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCg                        <  2:311451/93‑1 (MQ=255)

                                                       tATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCg                        >  2:1543272/1‑93 (MQ=255)

                                                       tATCCTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCg                        >  1:3909436/1‑93 (MQ=255)

                                                          ccTGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCa                     <  2:6880514/93‑1 (MQ=255)

                                                            tGACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGt                   >  1:1466321/1‑93 (MQ=255)

                                                             gACCACCATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGtt                  <  1:4649121/93‑1 (MQ=255)

                                                              accaccATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGttt                 >  2:5340919/1‑93 (MQ=255)

                                                              accaccATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGttt                 <  2:2201510/93‑1 (MQ=255)

                                                              accaccATATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGttt                 <  1:5544218/93‑1 (MQ=255)

                                                                caccaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGc               <  2:5766381/93‑1 (MQ=255)

                                                                caccaTATAAGCCGAGA‑ATCGGCGCGTTAAGATAGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGc               <  1:7601563/93‑1 (MQ=255)

                                                                 cccaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGcc              <  1:435164/92‑1 (MQ=255)

                                                                 accaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGcc              <  2:1289944/93‑1 (MQ=255)

                                                                 accaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGcc              <  1:2936757/93‑1 (MQ=255)

                                                                 accaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGcc              >  2:618739/1‑93 (MQ=255)

                                                                 accaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGcc              >  2:208406/1‑93 (MQ=255)

                                                                  ccaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCg             >  1:3228613/1‑93 (MQ=255)

                                                                  ccaTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCg             >  2:2615756/1‑93 (MQ=255)

                                                                   caTATAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGt            <  2:117030/93‑1 (MQ=255)

                                                                    atatAAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGACCCCCGCCAGTTTGCCGtc           >  1:2499639/1‑92 (MQ=255)

                                                                     tataAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTAc          <  1:3736304/93‑1 (MQ=255)

                                                                     tataAGATGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTAGTCCTCCGTCAGTTTGCCCTAc          >  2:4648705/1‑93 (MQ=255)

                                                                       taAGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCa        <  2:1826002/93‑1 (MQ=255)

                                                                         aGCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACg      >  1:6533976/1‑93 (MQ=255)

                                                                          gCCGAGA‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGc     >  2:3189954/1‑93 (MQ=255)

                                                                             gaga‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAc  <  1:5033548/93‑1 (MQ=255)

                                                                             gaga‑ATCGGCGCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAc  <  1:4986562/93‑1 (MQ=255)

TTAGCAGCCCGCAGCGCCTGGCGCATGGTTTCAACGCTCTCCTGCGGAATGCTGTTATCCTGACCACCATATAAGCCGAGA‑ATCCG‑GCGTTAAGATCGGTTGCGATATCAACAGGTTGTTTCGGTGAATTCAGCGACTTGTCGCCCGTCAGTTTGCCGTACCACGCCAC  >  NC_000913/4013092‑4013260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`