BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,045,192	C→T	A216V (GCC→GTC)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,045,192	0	C	T	100.0%	1285.7 / NA	357	A216V (GCC→GTC)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (193/164); total (193/164)

Only 100 of 363 total aligned reads displayed.

CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT  >  NC_000913/4045102‑4045281

cctcTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCg                                                                                         >  2:54910/1‑93 (MQ=255)

 ctctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGa                                                                                        >  1:1612507/1‑93 (MQ=255)

 ctctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGa                                                                                        <  1:601722/93‑1 (MQ=255)

 ctctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGa                                                                                        <  2:20979/93‑1 (MQ=255)

 ctctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGa                                                                                        >  1:2748051/1‑93 (MQ=255)

   ctGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGc                                                                                      >  1:4100401/1‑93 (MQ=255)

    tGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGcc                                                                                     <  2:3108966/93‑1 (MQ=255)

     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    <  2:1968546/93‑1 (MQ=255)

     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    >  2:4885408/1‑93 (MQ=255)

     gATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCa                                                                                    <  2:663643/93‑1 (MQ=255)

      aTAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAg                                                                                   >  2:7602284/1‑93 (MQ=255)

      aTAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAg                                                                                   >  1:4840576/1‑93 (MQ=255)

      aTAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAg                                                                                   >  2:1088158/1‑93 (MQ=255)

       tAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGa                                                                                  >  2:1147958/1‑93 (MQ=255)

        aaCATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaa                                                                                 >  2:6884082/1‑93 (MQ=255)

         aCATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTTGAGCCAGaaa                                                                                >  1:1261754/1‑93 (MQ=255)

            ttCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaaaa                                                                             >  2:2822075/1‑93 (MQ=255)

            ttCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGaaaaaa                                                                             >  2:6207128/1‑93 (MQ=255)

             tCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAAt                                                                            <  1:6444194/93‑1 (MQ=255)

             tCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAAt                                                                            >  1:5283695/1‑93 (MQ=255)

               cTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTg                                                                          >  1:2881807/1‑93 (MQ=255)

                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  1:6344762/93‑1 (MQ=255)

                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  2:3755335/93‑1 (MQ=255)

                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  2:3243365/93‑1 (MQ=255)

                  gCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTg                                                                       <  1:2623290/93‑1 (MQ=255)

                    gTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTggg                                                                     >  1:6583029/1‑93 (MQ=255)

                    gTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTggg                                                                     >  2:4714457/1‑93 (MQ=255)

                     tACCGGGCGTCGG‑GAAAAAACCGCGCAGGCGTTGCTGCAAGGTCTTGGCGGACTGGATACGCTGGATGTCGAGCCAGAAAAAATTGCTggggt                                                                   >  2:2738746/1‑91 (MQ=255)

                       ccGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTg                                                                  >  2:6574255/1‑93 (MQ=255)

                        cGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGa                                                                 >  1:7707771/1‑93 (MQ=255)

                          ggCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGc                                                               >  1:4585872/1‑93 (MQ=255)

                          ggCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGc                                                               >  2:1619182/1‑93 (MQ=255)

                          ggCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGc                                                               >  1:7490679/1‑93 (MQ=255)

                             gTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTc                                                            <  1:3524260/93‑1 (MQ=255)

                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           >  2:1452802/1‑93 (MQ=255)

                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           >  1:975738/1‑93 (MQ=255)

                              tCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTcc                                                           >  1:2332196/1‑93 (MQ=255)

                                ggTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGt                                                         <  1:3944874/93‑1 (MQ=255)

                                     aaaaaCCGCGCAGGCATTGCTGCAAGGTCGTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGgcgc                                                    <  1:3874285/93‑1 (MQ=255)

                                      aaaaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGcgcg                                                   >  2:7267723/1‑93 (MQ=255)

                                        aaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGaa                                                 >  2:5001883/1‑93 (MQ=255)

                                        aaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGaa                                                 <  2:1364974/93‑1 (MQ=255)

                                        aaCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGaa                                                 >  2:4400174/1‑93 (MQ=255)

                                         aCCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGaaa                                                >  2:3077340/1‑93 (MQ=255)

                                            gcgcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACa                                             >  2:5132966/1‑93 (MQ=255)

                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           <  1:2566343/93‑1 (MQ=255)

                                              gcAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAAt                                           <  1:722821/93‑1 (MQ=255)

                                                 ggCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGc                                        <  2:746381/93‑1 (MQ=255)

                                                  gCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCa                                       >  2:6703590/1‑93 (MQ=255)

                                                     ttGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGCAAACAATGGCAGCg                                    >  2:4162099/1‑93 (MQ=255)

                                                     ttGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCg                                    >  1:3049215/1‑93 (MQ=255)

                                                     ttGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCg                                    >  1:5121017/1‑93 (MQ=255)

                                                     ttGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGACAGCg                                    >  2:3797865/1‑93 (MQ=255)

                                                       gctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGaa                                  <  1:667698/93‑1 (MQ=255)

                                                        ctgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAg                                 >  1:6985113/1‑93 (MQ=255)

                                                         tgcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGc                                >  1:2413096/1‑93 (MQ=255)

                                                          gcAAGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCCGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCCAAgat                               >  1:2223653/1‑91 (MQ=255)

                                                            aaGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCg                             >  1:7107004/1‑93 (MQ=255)

                                                            aaGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCg                             >  2:7754725/1‑93 (MQ=255)

                                                            aaGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCg                             >  1:168032/1‑93 (MQ=255)

                                                            aaGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCg                             >  1:3321121/1‑93 (MQ=255)

                                                             aGGTCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGa                            <  2:4564427/93‑1 (MQ=255)

                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGCAGCTCGACCa                         >  2:7694726/1‑93 (MQ=255)

                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCa                         <  1:4678370/93‑1 (MQ=255)

                                                                tCTTGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCa                         <  2:5776032/93‑1 (MQ=255)

                                                                  ttGGCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCaaa                       >  2:3976625/1‑93 (MQ=255)

                                                                    ggCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAc                     >  1:1817421/1‑93 (MQ=255)

                                                                    ggCGGACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAAc                     <  1:803349/93‑1 (MQ=255)

                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  >  1:4493449/1‑93 (MQ=255)

                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  >  1:317444/1‑93 (MQ=255)

                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  >  2:5455222/1‑93 (MQ=255)

                                                                       ggACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGAGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACaaa                  >  2:7935757/1‑93 (MQ=255)

                                                                        gACTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaag                 >  1:4433967/1‑93 (MQ=255)

                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  1:1023822/93‑1 (MQ=255)

                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  2:6477112/93‑1 (MQ=255)

                                                                         aCTGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaaga                <  1:5468692/93‑1 (MQ=255)

                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              <  2:254472/93‑1 (MQ=255)

                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              >  2:2481138/1‑93 (MQ=255)

                                                                           tGGATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAaagaag              <  1:1907460/93‑1 (MQ=255)

                                                                            ggATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGt             <  1:5756192/93‑1 (MQ=255)

                                                                             gATACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGtt            <  1:5808464/93‑1 (MQ=255)

                                                                               tACGCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGc          >  1:7070318/1‑93 (MQ=255)

                                                                                  gCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTa       >  1:1131479/1‑93 (MQ=255)

                                                                                  gCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTa       <  1:4980592/93‑1 (MQ=255)

                                                                                  gCTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTa       <  1:67940/93‑1 (MQ=255)

                                                                                   cTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAt      <  2:6061810/93‑1 (MQ=255)

                                                                                   cTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAt      >  1:7821440/1‑93 (MQ=255)

                                                                                   cTGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAt      <  2:2633661/93‑1 (MQ=255)

                                                                                    tGTATGTCGAGCCAGACAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCACAACACAGAAGTTGCTTAtc     >  2:5284214/1‑93 (MQ=255)

                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     <  1:7641901/93‑1 (MQ=255)

                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     <  1:4791831/93‑1 (MQ=255)

                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     <  1:7657563/93‑1 (MQ=255)

                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     >  1:1516712/1‑93 (MQ=255)

                                                                                    tGTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtc     >  2:2916091/1‑93 (MQ=255)

                                                                                     gTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtct    >  2:6141115/1‑93 (MQ=255)

                                                                                     gTATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtct    >  1:4280716/1‑93 (MQ=255)

                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  2:3598851/93‑1 (MQ=255)

                                                                                      tATGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   >  2:5943723/1‑93 (MQ=255)

                                                                                      tATGCCGAGCAAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCTCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctc   <  1:1344131/93‑1 (MQ=255)

                                                                                       aTGTCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTAtctct  >  1:2976147/1‑93 (MQ=255)

CCTCTGATAACATTCCTGGCGTACCGGGCGTCGGTGAAAAAACCGCGCAGGCATTGCTGCAAGGTCTTGGCGGACTGGATACGCTGTATGCCGAGCCAGAAAAAATTGCTGGGTTGAGCTTCCGTGGCGCGAAAACAATGGCAGCGAAGCTCGAGCAAAACAAAGAAGTTGCTTATCTCT  >  NC_000913/4045102‑4045281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`