BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,041	A→G	S863P (TCC→CCC)	acrB ←	acridine efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,041	0	A	G	99.6%	815.3 / ‑5.3	261	S863P (TCC→CCC)	acrB	acridine efflux pump
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (125/135); total (125/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 272 total aligned reads displayed.

CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCC  >  NC_000913/480951‑481129

cGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCa                                                                                        <  2:1738453/93‑1 (MQ=255)

 gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCAt                                                                                       <  2:2188967/93‑1 (MQ=255)

  tACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATc                                                                                      <  1:3823297/93‑1 (MQ=255)

  tACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATc                                                                                      <  1:6876714/93‑1 (MQ=255)

  tACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATc                                                                                      >  2:2527745/1‑93 (MQ=255)

    cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATccc                                                                                    <  1:4197547/93‑1 (MQ=255)

     aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                   <  2:4749245/93‑1 (MQ=255)

     aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATcccc                                                                                   >  1:4966081/1‑93 (MQ=255)

       cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGt                                                                                 >  2:5808789/1‑93 (MQ=255)

         ccgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTcc                                                                               >  2:4275508/1‑93 (MQ=255)

          cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCa                                                                              <  1:5884571/93‑1 (MQ=255)

           gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAg                                                                             >  2:7167868/1‑93 (MQ=255)

           gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAg                                                                             >  1:3739325/1‑93 (MQ=255)

            ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGt                                                                            <  1:3646135/93‑1 (MQ=255)

              aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCa                                                                          <  1:7497211/93‑1 (MQ=255)

              aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCa                                                                          <  2:6403164/93‑1 (MQ=255)

              aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCa                                                                          >  1:2772187/1‑93 (MQ=255)

              aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCa                                                                          >  2:3217634/1‑93 (MQ=255)

               gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                         <  1:3915867/93‑1 (MQ=255)

               gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                         >  1:4858076/1‑93 (MQ=255)

               gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCAt                                                                         >  1:4699548/1‑93 (MQ=255)

                acacAGGAACACGACAATCAACGAAATGGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATa                                                                        <  1:6724520/93‑1 (MQ=255)

                acacAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATa                                                                        <  1:7126437/93‑1 (MQ=255)

                acacAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATa                                                                        <  2:512965/93‑1 (MQ=255)

                 cacaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAg                                                                       >  1:5978450/1‑93 (MQ=255)

                  acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGc                                                                      >  1:6379982/1‑93 (MQ=255)

                  acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGc                                                                      <  1:6198607/93‑1 (MQ=255)

                   caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGcc                                                                     <  1:4401687/93‑1 (MQ=255)

                   caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGcc                                                                     <  2:3342306/93‑1 (MQ=255)

                   caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGcc                                                                     >  2:1119248/1‑93 (MQ=255)

                     ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                   >  1:7566126/1‑93 (MQ=255)

                     ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                   <  1:7678499/93‑1 (MQ=255)

                     ggAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCaa                                                                   >  1:2489384/1‑93 (MQ=255)

                       aaCACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAaca                                                                 <  2:1198050/93‑1 (MQ=255)

                        acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAacac                                                                <  2:6204590/93‑1 (MQ=255)

                         cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCAGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACAcc                                                               >  1:6569081/1‑93 (MQ=255)

                          acGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCg                                                              >  1:7405751/1‑93 (MQ=255)

                           cGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATACCCGTCCAGTCATAGCCAACACCgg                                                             >  1:4175533/1‑93 (MQ=255)

                            gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGt                                                            >  1:919862/1‑93 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                           <  1:5476551/93‑1 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                           <  1:5169098/93‑1 (MQ=255)

                             aCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTa                                                           <  1:3165977/93‑1 (MQ=255)

                                aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                        <  2:1321459/93‑1 (MQ=255)

                                aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                        <  1:7047363/93‑1 (MQ=255)

                                 tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGcg                                                       >  2:2781177/1‑92 (MQ=255)

                                    aCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                    <  2:323464/93‑1 (MQ=255)

                                      gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                  <  2:3115292/93‑1 (MQ=255)

                                      gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                  >  2:813812/1‑93 (MQ=255)

                                      gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                  >  2:7780397/1‑93 (MQ=255)

                                      gAAATCGCGTAAAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                  >  2:4564789/1‑93 (MQ=255)

                                       aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                 >  1:7909584/1‑93 (MQ=255)

                                       aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                 >  1:1058517/1‑93 (MQ=255)

                                       aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGc                                                 <  1:6485820/93‑1 (MQ=255)

                                         aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                               >  2:7313252/1‑93 (MQ=255)

                                            gcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                            >  2:5680618/1‑93 (MQ=255)

                                                aCAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtt                                        <  2:5824143/93‑1 (MQ=255)

                                                   gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                     <  2:4449433/93‑1 (MQ=255)

                                                   gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                     >  2:6008212/1‑93 (MQ=255)

                                                      aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                  >  1:5429328/1‑93 (MQ=255)

                                                      aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                  <  1:2955527/93‑1 (MQ=255)

                                                      aaGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                  >  2:7672558/1‑93 (MQ=255)

                                                           gCCTGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGc                             >  2:2241360/1‑93 (MQ=255)

                                                              tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                          >  1:1818512/1‑93 (MQ=255)

                                                              tGGTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGCCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCACCtac                          >  2:406940/1‑91 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         <  2:6578874/93‑1 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         <  2:4622900/93‑1 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         <  1:3761901/93‑1 (MQ=255)

                                                               ggTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                         >  1:35855/1‑93 (MQ=255)

                                                                gTTGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                        <  2:7464664/93‑1 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                       <  1:7280432/93‑1 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                       <  1:2068601/93‑1 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                       <  1:2233077/93‑1 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                       <  2:2882640/93‑1 (MQ=255)

                                                                 ttGCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                       <  2:19580/93‑1 (MQ=255)

                                                                   gCCGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                     <  1:5669985/93‑1 (MQ=255)

                                                                    ccGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCt                    <  1:1929722/93‑1 (MQ=255)

                                                                     cGGAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCtt                   <  2:2948325/93‑1 (MQ=255)

                                                                      ggAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                  <  1:2527746/93‑1 (MQ=255)

                                                                      ggAGAGACGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                  <  2:6687038/93‑1 (MQ=255)

                                                                      cgagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                  <  1:2665966/92‑1 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                 <  2:3090003/93‑1 (MQ=255)

                                                                       gagagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCa                 >  2:6785696/1‑93 (MQ=255)

                                                                        agagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAc                >  1:5555244/1‑93 (MQ=255)

                                                                         gagaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGCTAGGCAGTTAGCTCGCCAGTTGTTCCCTAAGTTCCATTGCTTcgcg               >  2:4128172/1‑90 (MQ=255)

                                                                          agaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg              >  1:7933795/1‑93 (MQ=255)

                                                                          agaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg              >  2:1479285/1‑93 (MQ=255)

                                                                           gaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg             <  2:5498559/93‑1 (MQ=255)

                                                                           gaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg             >  2:4179026/1‑93 (MQ=255)

                                                                           gaCGTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCgg             <  2:2333438/93‑1 (MQ=255)

                                                                              gTTCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc          <  1:6821347/93‑1 (MQ=255)

                                                                                tCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtt        >  2:1271127/1‑93 (MQ=255)

                                                                                tCCTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtt        <  1:1536871/93‑1 (MQ=255)

                                                                                 ccTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACttt       >  2:4995385/1‑93 (MQ=255)

                                                                                 ccTGATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACttt       <  1:1277091/93‑1 (MQ=255)

                                                                                    gATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc    >  2:3790253/1‑93 (MQ=255)

                                                                                    gATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc    <  2:1659790/93‑1 (MQ=255)

                                                                                    gATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc    >  1:5796233/1‑93 (MQ=255)

                                                                                    gATAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAc    >  1:3499722/1‑93 (MQ=255)

                                                                                     aTAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc   >  2:3562396/1‑93 (MQ=255)

                                                                                     aTAGGGCATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc   <  1:241107/93‑1 (MQ=255)

CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCC  >  NC_000913/480951‑481129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`