Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,083,858 T→C V88A (GTT→GCT)  fdhD → affects formate dehydrogenase‑N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,083,8580TC99.7% 1120.2 / ‑5.3 360V88A (GTT→GCT) fdhDaffects formate dehydrogenase‑N
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (174/184);  total (175/185)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

Only 100 of 403 total aligned reads displayed.

GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGAC  >  NC_000913/4083769‑4083947
                                                                                         |                                                                                         
  ccAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctcctt                                                                                      >  1:6106763/1‑93 (MQ=255)
   cAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttc                                                                                     >  2:2857254/1‑93 (MQ=255)
    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttcc                                                                                    >  2:7601482/1‑93 (MQ=255)
    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttcc                                                                                    >  2:4313510/1‑93 (MQ=255)
    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGctccttcc                                                                                    >  2:3156391/1‑93 (MQ=255)
    aaaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTAGctccttcc                                                                                    <  1:4456316/93‑1 (MQ=255)
     aaGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCtccttcct                                                                                   >  1:1111194/1‑93 (MQ=255)
      aGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTg                                                                                  <  1:149116/93‑1 (MQ=255)
       gACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGt                                                                                 <  2:85331/93‑1 (MQ=255)
        aCCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTa                                                                                >  2:2154601/1‑93 (MQ=255)
         ccTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTaa                                                                               <  1:815893/93‑1 (MQ=255)
          cTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAAt                                                                              <  2:4271572/93‑1 (MQ=255)
          cTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAAt                                                                              <  1:3761221/93‑1 (MQ=255)
           ttGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATg                                                                             <  1:1254781/93‑1 (MQ=255)
           ttGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATg                                                                             <  1:3357882/93‑1 (MQ=255)
           ttGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGAATCGATGTCGTTCCTTCCTGAAATg                                                                             >  2:2374243/1‑93 (MQ=255)
            tGAGTACTTTGCGCTCGGTTTTTCGCTTTCGGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATgg                                                                            <  1:7128649/93‑1 (MQ=255)
             taGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGt                                                                           <  2:3236288/92‑1 (MQ=255)
             gAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGt                                                                           <  1:2330510/93‑1 (MQ=255)
             gAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGt                                                                           <  2:4180339/93‑1 (MQ=255)
              aGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGtt                                                                          >  2:6314266/1‑92 (MQ=255)
              aGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTc                                                                          >  1:1690035/1‑93 (MQ=255)
                tACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCtt                                                                        >  1:3177516/1‑93 (MQ=255)
                 aCTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTg                                                                       <  2:6161552/93‑1 (MQ=255)
                 aCTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTg                                                                       <  2:7131767/93‑1 (MQ=255)
                  cTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGa                                                                      >  1:3969891/1‑93 (MQ=255)
                  cTTTGCGCTCGGATTTTCGCTTTCCGGGGGGATTATCGCGAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGACTTGa                                                                      <  2:7041294/93‑1 (MQ=255)
                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     >  2:5782322/1‑93 (MQ=255)
                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     >  1:6480449/1‑93 (MQ=255)
                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     <  2:3645844/93‑1 (MQ=255)
                   tttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGaa                                                                     <  1:6246156/93‑1 (MQ=255)
                    ttGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAg                                                                    <  2:1302841/93‑1 (MQ=255)
                      gcgcTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTa                                                                  <  1:6974687/93‑1 (MQ=255)
                      gcgcTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTa                                                                  >  2:6814015/1‑93 (MQ=255)
                          tCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACaaa                                                              <  2:5882032/93‑1 (MQ=255)
                          ccGGTTTTTCGCTTTCCGAGGGGATTATCGGAAGTCCGCGCGATATCTGCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACaaa                                                              <  1:5597288/92‑1 (MQ=255)
                           ccgTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAt                                                             <  1:3224246/91‑1 (MQ=255)
                           cGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTGCTTCCTGTAATGGTCTTGAAGTACAAAt                                                             <  1:7542305/93‑1 (MQ=255)
                           cGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAt                                                             <  2:4609898/93‑1 (MQ=255)
                            ggTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAtt                                                            <  2:1198409/93‑1 (MQ=255)
                            ggTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAAtt                                                            >  2:2009876/1‑93 (MQ=255)
                             gTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTg                                                           >  1:6708615/1‑93 (MQ=255)
                               ttttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAg                                                         <  1:981003/93‑1 (MQ=255)
                                tttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGc                                                        <  2:7401305/93‑1 (MQ=255)
                                tttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGc                                                        <  1:2833619/93‑1 (MQ=255)
                                 ttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCt                                                       <  1:1357689/93‑1 (MQ=255)
                                 ttCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCt                                                       <  2:2534905/93‑1 (MQ=255)
                                 ttCGCGTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCt                                                       <  1:1007811/93‑1 (MQ=255)
                                  tCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCtt                                                      >  2:47479/1‑93 (MQ=255)
                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    <  1:7322357/93‑1 (MQ=255)
                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    <  2:7664914/93‑1 (MQ=255)
                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    >  2:2289428/1‑93 (MQ=255)
                                    gCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTc                                                    <  1:785083/93‑1 (MQ=255)
                                      tttCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCa                                                  <  2:4691104/93‑1 (MQ=255)
                                      tttCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCa                                                  >  2:5525669/1‑93 (MQ=255)
                                        tCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgc                                                <  1:4612210/93‑1 (MQ=255)
                                         ccGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgcc                                               <  1:3927939/93‑1 (MQ=255)
                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             >  2:4933563/1‑93 (MQ=255)
                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             >  1:5811447/1‑93 (MQ=255)
                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             <  2:2758040/93‑1 (MQ=255)
                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             >  1:3410994/1‑93 (MQ=255)
                                           gAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgc                                             <  1:1451168/93‑1 (MQ=255)
                                            agtggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            <  2:5323655/90‑1 (MQ=255)
                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            >  2:1861360/1‑93 (MQ=255)
                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            >  1:4267950/1‑93 (MQ=255)
                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAgccgcc                                            >  2:7353551/1‑93 (MQ=255)
                                            aGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTCAGCTTTCCAgccgcc                                            >  2:4450612/1‑93 (MQ=255)
                                             ggggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGccgccg                                           >  2:388653/1‑93 (MQ=255)
                                               ggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCt                                         >  2:4018676/1‑93 (MQ=255)
                                               ggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCt                                         >  1:5040755/1‑93 (MQ=255)
                                                gATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCtt                                        >  1:5410175/1‑93 (MQ=255)
                                                  ttATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTa                                      <  2:667383/93‑1 (MQ=255)
                                                    aTCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATg                                    <  1:1921879/93‑1 (MQ=255)
                                                     tCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgg                                   <  1:3679255/93‑1 (MQ=255)
                                                     tCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgg                                   >  2:1573448/1‑93 (MQ=255)
                                                     tCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgg                                   <  2:1697967/93‑1 (MQ=255)
                                                       gAAAGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATgggg                                 <  1:1847009/93‑1 (MQ=255)
                                                        aaaGTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGt                                <  1:4389200/93‑1 (MQ=255)
                                                           gTCCGCGCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGa                             <  1:6824539/93‑1 (MQ=255)
                                                             ccGCTCGATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAg                           <  2:5041449/93‑1 (MQ=255)
                                                               gcgcgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGa                         >  2:4542911/1‑93 (MQ=255)
                                                               gcgcgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGa                         <  1:591163/93‑1 (MQ=255)
                                                               gcgcgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGa                         <  2:3014739/93‑1 (MQ=255)
                                                                 gcgATATCTTCGGCATGGAGTTCGCTCCTTCCTGTAAGTGTCGTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAgc                       <  1:6385762/93‑1 (MQ=255)
                                                                  cgATATCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAgcg                      >  2:4109892/1‑93 (MQ=255)
                                                                    atatCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGcgcc                    >  1:6007533/1‑93 (MQ=255)
                                                                      atCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGcgccgc                  <  2:6469017/93‑1 (MQ=255)
                                                                      atCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGcgccgc                  <  1:3033268/93‑1 (MQ=255)
                                                                       tCTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCgccgcc                 >  1:4980405/1‑93 (MQ=255)
                                                                        cTTCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGccgccg                >  2:5598373/1‑93 (MQ=255)
                                                                         ttCGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCgg               <  1:2557164/93‑1 (MQ=255)
                                                                           cGGCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGgc             <  2:3493231/93‑1 (MQ=255)
                                                                            ggCATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGgcc            >  1:2207748/1‑92 (MQ=255)
                                                                              cATGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCt          >  2:6056315/1‑93 (MQ=255)
                                                                                tGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTgg        >  2:656937/1‑93 (MQ=255)
                                                                                tGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTgg        >  1:4829383/1‑93 (MQ=255)
                                                                                tGGATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTgg        >  2:1817937/1‑93 (MQ=255)
                                                                                  gATGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCg      >  1:7384420/1‑93 (MQ=255)
                                                                                    tGTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCggg    <  2:7765798/93‑1 (MQ=255)
                                                                                     gTCGCTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGa   >  2:6976846/1‑93 (MQ=255)
                                                                                         |                                                                                         
GCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGAC  >  NC_000913/4083769‑4083947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑