BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,088,657	T→C	I431V (ATC→GTC)	frvB ←	PTS system, fructose‑like enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,088,657	0	T	C	100.0%	1109.1 / NA	352	I431V (ATC→GTC)	frvB	PTS system, fructose‑like enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (182/170); total (182/170)

Only 100 of 380 total aligned reads displayed.

TAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCG  >  NC_000913/4088567‑4088746

tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGacac                                                                                         >  1:5954493/1‑93 (MQ=255)

tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGacac                                                                                         >  2:5151375/1‑93 (MQ=255)

 aatgaatgAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACAcc                                                                                        >  2:5788240/1‑93 (MQ=255)

   tgaatgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCa                                                                                      >  1:5423282/1‑93 (MQ=255)

    gaatgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCaa                                                                                     <  1:3812456/93‑1 (MQ=255)

     aatgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCaaa                                                                                    >  1:7777468/1‑93 (MQ=255)

      atgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAAc                                                                                   <  1:6493676/93‑1 (MQ=255)

       tgaaATGTTCAGCGCGAGGCCACTCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAAcc                                                                                  >  2:7568326/1‑93 (MQ=255)

       tgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAAcc                                                                                  <  2:6133873/93‑1 (MQ=255)

         aaaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAg                                                                                <  2:7372970/93‑1 (MQ=255)

          aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAgg                                                                               <  1:1065406/93‑1 (MQ=255)

            tGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTg                                                                             >  1:4818265/1‑93 (MQ=255)

            tGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTg                                                                             >  1:7036188/1‑93 (MQ=255)

            tGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTg                                                                             >  1:5505432/1‑93 (MQ=255)

             gTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGc                                                                            <  1:5292121/93‑1 (MQ=255)

             gTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGc                                                                            >  1:6382111/1‑93 (MQ=255)

               tCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCa                                                                          >  1:3995060/1‑93 (MQ=255)

               tCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCa                                                                          <  2:4845652/93‑1 (MQ=255)

               tCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGCGCCa                                                                          >  2:2210188/1‑93 (MQ=255)

                 aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGa                                                                        <  2:823020/93‑1 (MQ=255)

                 aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGa                                                                        >  2:2881905/1‑93 (MQ=255)

                 aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGa                                                                        <  1:7430000/93‑1 (MQ=255)

                 aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGa                                                                        <  1:4161093/93‑1 (MQ=255)

                  gcgcGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGAc                                                                       <  2:1192839/93‑1 (MQ=255)

                   cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACg                                                                      >  1:2749846/1‑93 (MQ=255)

                    gcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGt                                                                     <  1:1694089/93‑1 (MQ=255)

                     cgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGtt                                                                    <  2:3898031/93‑1 (MQ=255)

                      gAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGttt                                                                   <  2:3167870/93‑1 (MQ=255)

                       aGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTg                                                                  >  1:3579191/1‑93 (MQ=255)

                        ggCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGa                                                                 >  2:556908/1‑93 (MQ=255)

                         gCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGAt                                                                <  1:4672257/93‑1 (MQ=255)

                         gCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGAt                                                                <  2:1523649/93‑1 (MQ=255)

                         gCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGAt                                                                <  1:5480385/93‑1 (MQ=255)

                           cAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTc                                                              >  1:2631675/1‑93 (MQ=255)

                             aTCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCg                                                            >  1:2575873/1‑93 (MQ=255)

                              tCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGa                                                           >  1:230671/1‑93 (MQ=255)

                              tCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGa                                                           >  2:7215060/1‑93 (MQ=255)

                              tCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGa                                                           >  2:6685552/1‑93 (MQ=255)

                               cGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCgga                                                          >  1:4252096/1‑91 (MQ=255)

                                gCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGaaa                                                         <  2:7448863/93‑1 (MQ=255)

                                 ccAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGATGTGTGATTCCGAAAg                                                        >  2:7022900/1‑93 (MQ=255)

                                  cAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACAAGGTGCCAGACGTTTGATTCCGAAAGc                                                       >  2:4591650/1‑93 (MQ=255)

                                   aGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCg                                                      <  1:5848500/93‑1 (MQ=255)

                                    gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGa                                                     >  2:4028305/1‑93 (MQ=255)

                                    gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGa                                                     >  2:4416821/1‑93 (MQ=255)

                                    gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGa                                                     <  2:1703274/93‑1 (MQ=255)

                                    gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGa                                                     >  1:4955214/1‑93 (MQ=255)

                                     cACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGAt                                                    <  1:713222/93‑1 (MQ=255)

                                      aCCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATc                                                   >  1:2223687/1‑93 (MQ=255)

                                       ccAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCa                                                  >  1:1135877/1‑93 (MQ=255)

                                       ccAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCa                                                  >  2:6529263/1‑93 (MQ=255)

                                        cAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCAc                                                 <  1:7918929/93‑1 (MQ=255)

                                        cAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCAc                                                 >  2:2469860/1‑93 (MQ=255)

                                         aGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCAcc                                                >  2:2652599/1‑93 (MQ=255)

                                         aGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCAcc                                                >  2:3437244/1‑93 (MQ=255)

                                          gATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCa                                               <  1:6821777/93‑1 (MQ=255)

                                          gATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCa                                               <  2:1427146/93‑1 (MQ=255)

                                           aTAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAg                                              >  1:1284510/1‑93 (MQ=255)

                                            tAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGc                                             >  1:1768400/1‑93 (MQ=255)

                                               aaCGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACg                                          >  2:18527/1‑93 (MQ=255)

                                               aaCGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACg                                          >  2:2295570/1‑93 (MQ=255)

                                                aCGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGc                                         <  1:7579282/93‑1 (MQ=255)

                                                 cGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGcc                                        >  2:1121651/1‑93 (MQ=255)

                                                   aaCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCgg                                      >  2:6801963/1‑93 (MQ=255)

                                                       cACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAAt                                  <  1:2023573/93‑1 (MQ=255)

                                                       cACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAAt                                  <  1:5217184/93‑1 (MQ=255)

                                                       cACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAAt                                  <  2:1272100/93‑1 (MQ=255)

                                                       cACCGGCGACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAAt                                  <  1:6571958/93‑1 (MQ=255)

                                                       cACCGGCGACATCAGGCCGATCAGCGGCTCAAAGACTCCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAAt                                  <  1:634567/93‑1 (MQ=255)

                                                         ccGGCGACATCAGGCCGATCAGCGGCTGAAAGACACCCAAGCCAGGTGCGAGACGTTTGATCCCGAAAGCCATCACCAGCACGCCGGTAACgc                                <  2:4166563/93‑1 (MQ=255)

                                                              gACATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGccgccc                           >  2:5409704/1‑93 (MQ=255)

                                                               aCATCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGccgcccg                          >  1:3111702/1‑93 (MQ=255)

                                                                  tCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCa                       >  2:58822/1‑93 (MQ=255)

                                                                  tCAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGATTGATTCCGAAAGCGATCATCTGAACGCCAGTAATGCCGTCCGCCa                       >  2:4083450/1‑93 (MQ=255)

                                                                  tCAGGCCCATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCa                       <  1:2360307/93‑1 (MQ=255)

                                                                   cAGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAg                      >  2:7863014/1‑93 (MQ=255)

                                                                    aGGCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGc                     >  1:871966/1‑93 (MQ=255)

                                                                     ggCCGATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCg                    <  1:4473685/93‑1 (MQ=255)

                                                                         gATCAGCGGGTCAAAGACACCCAACCCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTAtt                <  1:3184763/93‑1 (MQ=255)

                                                                         gATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTAtt                <  1:5670415/93‑1 (MQ=255)

                                                                         gATCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTAtt                <  2:5023479/93‑1 (MQ=255)

                                                                          aTCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCCCCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTg               <  2:6067532/93‑1 (MQ=255)

                                                                          aTCAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTg               <  2:2727004/93‑1 (MQ=255)

                                                                            cAGCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGc             >  1:369576/1‑93 (MQ=255)

                                                                              gCGGGTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCgg           >  1:7346025/1‑93 (MQ=255)

                                                                               cGGGTCAAAGACACCCAAACCAGGTGCCAGCCGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGt          <  2:3272661/93‑1 (MQ=255)

                                                                                 ggTCAAAGACACCCAACCCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTaa        <  1:1473830/93‑1 (MQ=255)

                                                                                 ggTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTaa        <  2:2355834/93‑1 (MQ=255)

                                                                                 ggTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTaa        <  2:3811347/93‑1 (MQ=255)

                                                                                 ggTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTaa        <  1:2120392/93‑1 (MQ=255)

                                                                                  gTCAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAat       <  1:6008140/93‑1 (MQ=255)

                                                                                    cAAAGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatca     <  2:3625568/93‑1 (MQ=255)

                                                                                     aaaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcat    >  1:512508/1‑93 (MQ=255)

                                                                                     aaaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcat    <  1:6919637/93‑1 (MQ=255)

                                                                                     aaaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcat    >  2:2134624/1‑93 (MQ=255)

                                                                                     aaaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcat    >  2:2595712/1‑93 (MQ=255)

                                                                                      aaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcatc   >  1:6166577/1‑93 (MQ=255)

                                                                                      aaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcatc   >  1:1811166/1‑93 (MQ=255)

                                                                                      aaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcatc   <  2:1053460/93‑1 (MQ=255)

                                                                                      aaGACACCCAAACCAGGTGCCAGACGTTTGATTCCGAAACCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAatcatc   >  2:7678982/1‑93 (MQ=255)

TAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCAGGTGCCAGACGTTTGATTCCGAAAGCGATCACCAGCACGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCG  >  NC_000913/4088567‑4088746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`