BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,105,759	A→G	I210V (ATC→GTC)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,105,759	0	A	G	99.4%	1072.4 / ‑3.7	351	I210V (ATC→GTC)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (163/186); total (163/188)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

Only 100 of 366 total aligned reads displayed.

GCTGCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTATCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACC  >  NC_000913/4105669‑4105848

   gCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTcgcg                                                                                      >  1:5280904/1‑93 (MQ=255)

    cGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGa                                                                                     <  1:4072580/93‑1 (MQ=255)

    cGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGa                                                                                     <  1:4871331/93‑1 (MQ=255)

      gCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGaaa                                                                                   >  1:857811/1‑93 (MQ=255)

      gCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGaaa                                                                                   >  2:5039858/1‑93 (MQ=255)

         aTTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAgga                                                                                >  1:6696971/1‑92 (MQ=255)

          ttGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTa                                                                               >  2:260091/1‑93 (MQ=255)

          ttGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTa                                                                               >  2:2804091/1‑93 (MQ=255)

          ttGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTa                                                                               <  1:7194772/93‑1 (MQ=255)

          ttGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTa                                                                               >  1:2368846/1‑93 (MQ=255)

            tccGTTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaa                                                                             <  2:6947265/92‑1 (MQ=255)

              cGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTaaaaa                                                                           >  1:3253100/1‑93 (MQ=255)

                gTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAac                                                                         >  2:240121/1‑93 (MQ=255)

                  ggCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAacac                                                                       <  1:7348295/93‑1 (MQ=255)

                  ggCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAacac                                                                       <  1:6384066/93‑1 (MQ=255)

                   gCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACAcg                                                                      >  2:7656395/1‑93 (MQ=255)

                    gtgtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGGGGGTGTCGCGAAAGGTAAAAAACAcgc                                                                     <  2:4047141/92‑1 (MQ=255)

                    cTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACAcgc                                                                     >  1:1372991/1‑93 (MQ=255)

                     tgtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACAcgcg                                                                    >  2:1993138/1‑93 (MQ=255)

                      gtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGa                                                                   >  1:5127394/1‑93 (MQ=255)

                      gtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGa                                                                   <  2:3959387/93‑1 (MQ=255)

                      gtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGa                                                                   <  2:759683/93‑1 (MQ=255)

                      gtgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGa                                                                   >  2:7861997/1‑93 (MQ=255)

                       tgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGAt                                                                  >  2:4830543/1‑93 (MQ=255)

                       tgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGAt                                                                  <  2:4993554/93‑1 (MQ=255)

                       tgAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGAt                                                                  <  1:6630742/93‑1 (MQ=255)

                         aaTTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCg                                                                >  2:6048527/1‑93 (MQ=255)

                           ttCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTg                                                              <  1:955740/93‑1 (MQ=255)

                           ttCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTg                                                              <  1:4792144/93‑1 (MQ=255)

                            tCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTgg                                                             <  2:5371936/93‑1 (MQ=255)

                             cGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGc                                                            <  2:6446312/93‑1 (MQ=255)

                              gTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCg                                                           >  2:1550993/1‑93 (MQ=255)

                              gTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCg                                                           >  2:6316028/1‑93 (MQ=255)

                                 gtgGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGAtt                                                        <  1:1166329/93‑1 (MQ=255)

                                 gtgGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGAtt                                                        >  2:7165929/1‑93 (MQ=255)

                                 gtgGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGAtt                                                        <  1:1827576/93‑1 (MQ=255)

                                    gTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTAcc                                                     >  2:1218520/1‑93 (MQ=255)

                                    gTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTAcc                                                     >  2:3394132/1‑93 (MQ=255)

                                    gTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTAcc                                                     >  2:5218598/1‑93 (MQ=255)

                                    gTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTAcc                                                     >  1:75972/1‑93 (MQ=255)

                                     ttCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCg                                                    >  2:2935134/1‑93 (MQ=255)

                                      tCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGa                                                   <  1:42828/93‑1 (MQ=255)

                                       ccGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGaa                                                  <  1:5871152/93‑1 (MQ=255)

                                          gAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACat                                               <  1:4629164/93‑1 (MQ=255)

                                          gAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACat                                               >  1:770913/1‑93 (MQ=255)

                                           aaGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACata                                              >  1:3628857/1‑93 (MQ=255)

                                            aGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACatat                                             <  1:1492716/93‑1 (MQ=255)

                                            aGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACatat                                             >  1:6532600/1‑93 (MQ=255)

                                            aGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGGGTCGCGAAAGGGAAAAAACACGCGATCGTGGCGATTACCGAACatag                                             >  1:3883011/1‑92 (MQ=255)

                                             gTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtg                                            <  2:7260231/93‑1 (MQ=255)

                                             gTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtg                                            <  2:3029414/93‑1 (MQ=255)

                                              ttGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgt                                           <  2:2686210/93‑1 (MQ=255)

                                               tGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtg                                          <  2:6825153/93‑1 (MQ=255)

                                               tGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtg                                          <  2:4285385/93‑1 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         <  1:5422441/93‑1 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         <  2:6521479/93‑1 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         >  2:6679495/1‑93 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         <  2:4455636/93‑1 (MQ=255)

                                                gAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATAtgtgt                                         >  1:1198038/1‑93 (MQ=255)

                                                  aTTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGa                                       <  1:2102612/93‑1 (MQ=255)

                                                  aTTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGa                                       <  2:2206940/93‑1 (MQ=255)

                                                  aTTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGa                                       >  1:6038505/1‑93 (MQ=255)

                                                   ttCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGAt                                      <  1:435316/93‑1 (MQ=255)

                                                   ttCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGAt                                      <  2:113978/93‑1 (MQ=255)

                                                   ttCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGAt                                      <  2:307874/93‑1 (MQ=255)

                                                    tCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATg                                     >  1:7556938/1‑93 (MQ=255)

                                                      aGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGtt                                   <  1:616184/93‑1 (MQ=255)

                                                      aGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGtt                                   >  1:1700219/1‑93 (MQ=255)

                                                      aGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGtt                                   <  2:1403948/93‑1 (MQ=255)

                                                        ccGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGa                                 <  1:3969941/93‑1 (MQ=255)

                                                        ccGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGa                                 <  2:520148/93‑1 (MQ=255)

                                                        ccGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGa                                 <  2:1028073/93‑1 (MQ=255)

                                                         cGTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGAc                                >  1:4048815/1‑93 (MQ=255)

                                                          gTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACg                               >  1:7135144/1‑93 (MQ=255)

                                                          gTGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACg                               >  2:4705828/1‑93 (MQ=255)

                                                           tGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGa                              >  1:5948584/1‑93 (MQ=255)

                                                           tGAAGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGa                              <  2:1886517/93‑1 (MQ=255)

                                                             aaGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAAc                            <  1:5849521/93‑1 (MQ=255)

                                                             aaGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAAc                            <  1:2719162/93‑1 (MQ=255)

                                                             aaGACCAGGTAAACGAAGTCAAAGCGGATGTCGCAAAAGGTAAGTAGCACGCGATCGTGGCAATTACAGAACATATGTGTGATGTTGACGAAc                            <  1:7034398/93‑1 (MQ=255)

                                                              aGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACt                           <  2:4568241/93‑1 (MQ=255)

                                                              aGACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACt                           <  2:5707654/93‑1 (MQ=255)

                                                               gACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTg                          <  1:5539176/93‑1 (MQ=255)

                                                               gACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTg                          <  2:5838624/93‑1 (MQ=255)

                                                               gACCTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTg                          >  2:3496546/1‑93 (MQ=255)

                                                                  cTGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgcg                       >  1:2550644/1‑93 (MQ=255)

                                                                   tGGTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgcgc                      >  1:4817347/1‑93 (MQ=255)

                                                                   aggTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGgcgc                      <  1:1569263/92‑1 (MQ=255)

                                                                     gTAAACGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCAt                    >  2:3802933/1‑93 (MQ=255)

                                                                        aaCGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTc                 >  1:3343440/1‑93 (MQ=255)

                                                                         aCGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCa                <  1:1105353/93‑1 (MQ=255)

                                                                          cGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCAt               >  1:2952007/1‑93 (MQ=255)

                                                                          cGAAATCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCAt               >  1:3737006/1‑93 (MQ=255)

                                                                             aaTCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCga            >  1:199352/1‑93 (MQ=255)

                                                                              aTCAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCgag           >  1:7791677/1‑93 (MQ=255)

                                                                                cAAAGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaa         >  1:6818387/1‑93 (MQ=255)

                                                                                 aaaGCGGGTGTTGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaa        <  2:2545790/93‑1 (MQ=255)

                                                                                   aGCGGGTGTCGCGACAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaaga      <  2:3665330/93‑1 (MQ=255)

                                                                                   aGCGGGTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGagaaaga      <  1:6304584/93‑1 (MQ=255)

                                                                                      gggTGTCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAAc   <  2:7005011/93‑1 (MQ=255)

GCTGCGGCCATTGCCGGTGGCTGTGAATTCGTTGTGGTTCCGGAAGTTGAATTCAGCCGTGAAGACCTGGTAAACGAAATCAAAGCGGGTATCGCGAAAGGTAAAAAACACGCGATCGTGGCGATTACCGAACATATGTGTGATGTTGACGAACTGGCGCATTTCATCGAGAAAGAAACC  >  NC_000913/4105669‑4105848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`