BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	486,435	A→G	T226A (ACC→GCC)	aefA →	putative alpha helix protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	486,435	0	A	G	100.0%	856.5 / NA	272	T226A (ACC→GCC)	aefA	putative alpha helix protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (115/157); total (115/157)

Only 100 of 279 total aligned reads displayed.

CTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAA  >  NC_000913/486344‑486524

 ttACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGcc                                                                                         >  1:1056843/1‑93 (MQ=255)

   aCGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGt                                                                                       <  2:2354277/93‑1 (MQ=255)

    cGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTc                                                                                      <  1:2053160/93‑1 (MQ=255)

        ccagccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGc                                                                                  >  2:4122099/1‑93 (MQ=255)

         cagccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCa                                                                                 <  1:6061929/93‑1 (MQ=255)

           gccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAgg                                                                               >  2:6314573/1‑93 (MQ=255)

            ccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGa                                                                              <  1:7212908/93‑1 (MQ=255)

            ccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGa                                                                              <  1:7015823/93‑1 (MQ=255)

            ccagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGa                                                                              <  2:3002050/93‑1 (MQ=255)

             cagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGAt                                                                             <  1:4104226/93‑1 (MQ=255)

             cagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGAt                                                                             >  2:6202659/1‑93 (MQ=255)

             cagAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGAt                                                                             <  2:5456298/93‑1 (MQ=255)

              agAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATa                                                                            >  1:5028478/1‑93 (MQ=255)

              agAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATa                                                                            <  1:1255148/93‑1 (MQ=255)

               gAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAc                                                                           >  1:297745/1‑93 (MQ=255)

               gAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAc                                                                           >  2:3395396/1‑93 (MQ=255)

                aaaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATAcc                                                                          >  2:7480840/1‑93 (MQ=255)

                 aaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCt                                                                         >  1:6453780/1‑93 (MQ=255)

                 aaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCt                                                                         <  2:4331297/93‑1 (MQ=255)

                 aaGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCt                                                                         <  1:4737781/93‑1 (MQ=255)

                  aGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCtt                                                                        <  2:3518261/93‑1 (MQ=255)

                  aGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCtt                                                                        <  2:1503450/93‑1 (MQ=255)

                   gtgtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTg                                                                       >  1:3285549/1‑93 (MQ=255)

                   gtgtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTg                                                                       <  1:7223089/93‑1 (MQ=255)

                    tgtTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGc                                                                      <  1:3208426/93‑1 (MQ=255)

                          tGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGc                                                                <  2:1469760/93‑1 (MQ=255)

                           gCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCa                                                               <  2:6749390/93‑1 (MQ=255)

                           gCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCa                                                               <  1:7918722/93‑1 (MQ=255)

                            cAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCaa                                                              <  2:460984/93‑1 (MQ=255)

                            cAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCaa                                                              <  2:155987/93‑1 (MQ=255)

                             atgCCCAGCAGGCGTGGCTGAATGCGGAGATTGACCAGCAGGGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAAc                                                             <  1:2641408/91‑1 (MQ=255)

                             aGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAAc                                                             >  2:1889455/1‑93 (MQ=255)

                             aGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAAc                                                             <  2:854329/93‑1 (MQ=255)

                             aGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAAc                                                             <  2:6073097/93‑1 (MQ=255)

                              ggCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGACCGCCGTCTTGCAGGATACCTTGCAACAGCAACg                                                            >  2:7775820/1‑93 (MQ=255)

                                  cagcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGAt                                                        >  2:1065318/1‑93 (MQ=255)

                                   agcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGAtt                                                       >  2:4513359/1‑93 (MQ=255)

                                    gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTa                                                      >  1:5784703/1‑93 (MQ=255)

                                    gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTa                                                      <  1:4526154/93‑1 (MQ=255)

                                    gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTa                                                      <  1:218005/93‑1 (MQ=255)

                                     gacgCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGACACCTTGCAAAAGCAACGTGATTAc                                                     <  1:1136085/90‑1 (MQ=255)

                                     cagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTAc                                                     <  1:4508072/93‑1 (MQ=255)

                                       ggCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGt                                                   >  1:3967246/1‑93 (MQ=255)

                                       ggCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGt                                                   >  2:5118359/1‑93 (MQ=255)

                                          gTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGAc                                                <  1:3176341/93‑1 (MQ=255)

                                            tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACgg                                              >  2:5718852/1‑93 (MQ=255)

                                             gCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGc                                             >  2:4732199/1‑93 (MQ=255)

                                                  aTGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACa                                        >  1:5379164/1‑93 (MQ=255)

                                                  aTGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACa                                        <  2:1210844/93‑1 (MQ=255)

                                                   tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAg                                       <  1:3836963/93‑1 (MQ=255)

                                                    gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgc                                      <  2:1289537/93‑1 (MQ=255)

                                                    gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgc                                      >  1:3666702/1‑93 (MQ=255)

                                                     cGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgcg                                     <  1:1007772/93‑1 (MQ=255)

                                                      ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGCCGGCGAACAgcgc                                    >  2:2242848/1‑93 (MQ=255)

                                                      ggAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAgcgc                                    >  1:1544974/1‑93 (MQ=255)

                                                       gagaTTGACCAGCAGCGTAAAAGTCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCt                                   <  2:4287153/93‑1 (MQ=255)

                                                       gagaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCt                                   <  2:2077564/93‑1 (MQ=255)

                                                       gagaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGGGATTACGTGACGGCGAACAGCGCt                                   >  2:3194611/1‑93 (MQ=255)

                                                        agaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTc                                  >  1:2419527/1‑93 (MQ=255)

                                                          aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGt                                >  2:535023/1‑93 (MQ=255)

                                                           ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTc                               >  2:919385/1‑93 (MQ=255)

                                                           ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTc                               <  2:1342466/93‑1 (MQ=255)

                                                           ttGACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTc                               <  2:6660280/93‑1 (MQ=255)

                                                             gACCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTg                             <  2:333227/93‑1 (MQ=255)

                                                              aCCAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTgg                            <  1:3353402/93‑1 (MQ=255)

                                                               ccAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGa                           >  1:2907336/1‑93 (MQ=255)

                                                               ccAGCAGCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGa                           >  1:4226671/1‑93 (MQ=255)

                                                                cagcagCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAg                          <  2:3473607/93‑1 (MQ=255)

                                                                cagcagCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAg                          >  1:6252886/1‑93 (MQ=255)

                                                                cagcagCGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAg                          <  1:5123183/93‑1 (MQ=255)

                                                                  gcagcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCa                        <  2:492816/93‑1 (MQ=255)

                                                                     gcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCa                     <  1:6685904/93‑1 (MQ=255)

                                                                     gcGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCa                     >  2:2869088/1‑93 (MQ=255)

                                                                      cGTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAg                    >  2:6889229/1‑93 (MQ=255)

                                                                       gTAAAAGCCTGGAGGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCCACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGt                   >  2:3502212/1‑93 (MQ=255)

                                                                       gTAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGt                   >  1:2578475/1‑93 (MQ=255)

                                                                        tAAAAGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGGACCAGtt                  >  1:5886762/1‑93 (MQ=255)

                                                                         aaaaGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTa                 >  1:4329781/1‑93 (MQ=255)

                                                                         aaaaGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTa                 >  2:2269616/1‑93 (MQ=255)

                                                                          aaaGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTAc                >  2:78534/1‑93 (MQ=255)

                                                                          aaaGCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTAc                >  2:6092035/1‑93 (MQ=255)

                                                                             gCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAAc             <  2:7250888/93‑1 (MQ=255)

                                                                             gCCTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAAc             <  1:920472/93‑1 (MQ=255)

                                                                              ccTGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACt            <  2:3889617/93‑1 (MQ=255)

                                                                                tGGAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGt          <  2:3620629/93‑1 (MQ=255)

                                                                                 ggAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTTCAACTGtt         >  2:7011097/1‑93 (MQ=255)

                                                                                 ggAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGtt         >  1:6863155/1‑93 (MQ=255)

                                                                                 ggAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGtt         >  2:483457/1‑93 (MQ=255)

                                                                                 ggAAGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTCACAACTGtt         <  2:5241490/93‑1 (MQ=255)

                                                                                    aGGGAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCa      >  1:7723311/1‑93 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     >  2:6081052/1‑93 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     >  2:5909311/1‑93 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     <  1:3623522/93‑1 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     <  2:7319540/93‑1 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     <  1:1609556/93‑1 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     <  1:5580771/93‑1 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     <  1:6702016/93‑1 (MQ=255)

                                                                                     gggAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaa     <  1:978976/93‑1 (MQ=255)

                                                                                      ggAACGCCGTCTTGCAGGATACCTTTCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaag    >  1:5006777/1‑93 (MQ=255)

                                                                                       gAACGCCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCaaga   <  1:2264524/93‑1 (MQ=255)

CTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAA  >  NC_000913/486344‑486524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`