BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,799	T→C	V462A (GTA→GCA)	metL →	aspartokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,799	0	T	C	99.1%	1079.6 / ‑5.8	345	V462A (GTA→GCA)	metL	aspartokinase II
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (167/174); total (169/176)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTT  >  NC_000913/4128709‑4128889

tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                          >  2:7929589/1‑93 (MQ=255)

tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                          >  2:5567712/1‑93 (MQ=255)

tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                          <  2:263873/93‑1 (MQ=255)

tggtggCAGCACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                          >  2:2740307/1‑93 (MQ=255)

 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAAGGCATCGGCCTGGCAtt                                                                                         >  2:3996384/1‑93 (MQ=255)

 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                         >  2:6277277/1‑93 (MQ=255)

 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                         >  2:5138702/1‑93 (MQ=255)

  gtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTg                                                                                        >  2:3617075/1‑93 (MQ=255)

   tggCAGTACTGCGCGCCGGCGCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCATAAAAACGCATCGGCCTGGCATTGt                                                                                       <  1:6938916/93‑1 (MQ=255)

      cAGTACTGCGCACCGGCCCGACCGAAAGCTTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCg                                                                                    <  1:3864147/93‑1 (MQ=255)

       aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgg                                                                                   <  1:2388436/93‑1 (MQ=255)

        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgga                                                                                  >  2:1087346/1‑92 (MQ=255)

        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                  >  2:4828573/1‑93 (MQ=255)

          aCTGCGCACGGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAACCGCATCGGCCTGGCATTGTTCGGTaa                                                                                <  1:4598364/93‑1 (MQ=255)

          aCTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGAGCTGCATCAGTCCGTCTTCCACGCAGAAAAACGCATCGGCCTGGCATTGTTAGTTaa                                                                                >  2:6753117/1‑93 (MQ=255)

           cTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAg                                                                               <  1:2473659/93‑1 (MQ=255)

           cTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAg                                                                               >  1:2122053/1‑93 (MQ=255)

           cTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAg                                                                               >  1:4776348/1‑93 (MQ=255)

            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                              >  2:4073897/1‑93 (MQ=255)

            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTATTCCGCGCAGAAAAAAGCATCAGCCTGGCTTTGTTTGGGAAgg                                                                              >  2:1876116/1‑93 (MQ=255)

             gcgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAggg                                                                             <  2:1449104/93‑1 (MQ=255)

              cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGc                                                                            <  2:1303782/93‑1 (MQ=255)

                cACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCaa                                                                          <  2:5892434/93‑1 (MQ=255)

                cACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGATAAGGGCaa                                                                          >  1:5671239/1‑93 (MQ=255)

                 aCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAat                                                                         <  1:2519852/93‑1 (MQ=255)

                 aCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAat                                                                         >  1:6701481/1‑93 (MQ=255)

                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                        <  1:4178573/93‑1 (MQ=255)

                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                        >  1:7689755/1‑93 (MQ=255)

                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                      <  1:7593616/93‑1 (MQ=255)

                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                      <  1:6633380/93‑1 (MQ=255)

                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                     >  1:1618384/1‑93 (MQ=255)

                       ccgaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGt                                                                   <  2:5184160/93‑1 (MQ=255)

                       ccgaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGt                                                                   >  2:1809582/1‑93 (MQ=255)

                        cgaccgcAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGtt                                                                  <  2:7042028/93‑1 (MQ=255)

                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                 >  2:7764820/1‑93 (MQ=255)

                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                 >  2:7275028/1‑93 (MQ=255)

                          gccgaAAGCGTGATTCAGGGGCTGCATCAGTCCGTCTGCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTcc                                                                <  1:5491759/92‑1 (MQ=255)

                          accgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTcc                                                                <  2:3919838/93‑1 (MQ=255)

                           ccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTccc                                                               <  2:4864995/93‑1 (MQ=255)

                            cgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCg                                                              >  2:457091/1‑93 (MQ=255)

                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                             >  2:3437634/1‑93 (MQ=255)

                              aaaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGtt                                                            <  2:2239001/93‑1 (MQ=255)

                               aaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTg                                                           <  1:7450123/93‑1 (MQ=255)

                               aaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTg                                                           <  1:5334599/93‑1 (MQ=255)

                                 gCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCCCGCAGAAAAACGCATCTGCCTGGCATTGTTCGGTAACGGCAATATTGGTTCCCGttccc                                                         >  2:6632256/1‑90 (MQ=255)

                                   cTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTg                                                       >  1:1312488/1‑93 (MQ=255)

                                    tGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTgg                                                      >  2:7577847/1‑93 (MQ=255)

                                      atgcAGGGGCTGCATCAGTCCGTCGTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGaa                                                    <  1:374863/90‑1 (MQ=255)

                                       ttCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAc                                                   <  2:3712059/93‑1 (MQ=255)

                                         cAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTg                                                 <  1:7418673/93‑1 (MQ=255)

                                          cggggCTGCATCAGTCCCGCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGt                                                <  2:5699700/92‑1 (MQ=255)

                                          aGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGt                                                >  1:5896867/1‑93 (MQ=255)

                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                               >  1:2662297/1‑93 (MQ=255)

                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                               <  2:2125007/93‑1 (MQ=255)

                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                               <  2:5799631/93‑1 (MQ=255)

                                            gggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTc                                              >  1:6418311/1‑93 (MQ=255)

                                              gCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGc                                            >  2:6912489/1‑93 (MQ=255)

                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                         >  1:6267856/1‑93 (MQ=255)

                                                  cATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTTGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGt                                        >  2:1192977/1‑93 (MQ=255)

                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                       >  1:796413/1‑93 (MQ=255)

                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                       <  2:3817321/93‑1 (MQ=255)

                                                      aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagc                                    >  1:7828599/1‑93 (MQ=255)

                                                       gTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagca                                   <  2:4525083/93‑1 (MQ=255)

                                                        tCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcag                                  >  2:2369088/1‑93 (MQ=255)

                                                          cgccTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCGGTTGGCTGGAACTGTTCGCCCGTgagcggag                                <  2:2945106/90‑1 (MQ=255)

                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                                >  1:2313713/1‑93 (MQ=255)

                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                                >  1:7641447/1‑93 (MQ=255)

                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                               >  1:3067511/1‑93 (MQ=255)

                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                               >  2:4736257/1‑93 (MQ=255)

                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                               >  2:7307324/1‑93 (MQ=255)

                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                               <  1:1106531/93‑1 (MQ=255)

                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                              <  2:5303752/93‑1 (MQ=255)

                                                             cTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagcgc                             >  1:1853543/1‑91 (MQ=255)

                                                              ttCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACg                            >  1:5138861/1‑93 (MQ=255)

                                                               tCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGc                           <  1:2202144/93‑1 (MQ=255)

                                                               tCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGc                           >  1:4133988/1‑93 (MQ=255)

                                                               tCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGc                           >  1:6812672/1‑93 (MQ=255)

                                                               tCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGc                           >  2:2014267/1‑93 (MQ=255)

                                                                ccGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCt                          <  1:1356843/93‑1 (MQ=255)

                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                        <  2:1573382/93‑1 (MQ=255)

                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                       <  1:7906162/93‑1 (MQ=255)

                                                                     cAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCgg                     <  1:4296546/93‑1 (MQ=255)

                                                                        aaaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAc                  <  2:4420411/93‑1 (MQ=255)

                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                 >  2:7101845/1‑93 (MQ=255)

                                                                              gCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCg            <  1:4890552/93‑1 (MQ=255)

                                                                               cATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCgg           >  2:425067/1‑93 (MQ=255)

                                                                                aTCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGAACGCTTTCGGCACGTACCGGc          >  1:3456289/1‑93 (MQ=255)

                                                                                 tCGGCCTGGCATTGTTCGGTACGGGCAATATCGGTTCCCGTTGGCTTGAACTGTTCTCCCGCGAGAAGAGCACGCTTTCTGCACGTACCGACt         >  2:279202/1‑93 (MQ=255)

                                                                                 tCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCt         <  2:3173387/93‑1 (MQ=255)

                                                                                 tCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCt         <  1:6074637/93‑1 (MQ=255)

                                                                                  cGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCtt        <  2:5098066/93‑1 (MQ=255)

                                                                                  cGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCtt        <  1:7150478/93‑1 (MQ=255)

                                                                                   ggCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCttt       <  2:2909078/93‑1 (MQ=255)

                                                                                   ggCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCttt       <  1:1024754/93‑1 (MQ=255)

                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg      <  2:6370082/93‑1 (MQ=255)

                                                                                     ccTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGa     <  2:2994430/93‑1 (MQ=255)

                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGat    >  1:1571015/1‑92 (MQ=255)

                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAg    >  1:179035/1‑93 (MQ=255)

                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt   >  1:3532029/1‑93 (MQ=255)

                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt   >  1:3598750/1‑93 (MQ=255)

TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTT  >  NC_000913/4128709‑4128889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`