BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,149,444	A→G	Y412H (TAC→CAC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,149,444	0	A	G	100.0%	1102.9 / NA	352	Y412H (TAC→CAC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (184/168); total (184/168)

Only 100 of 371 total aligned reads displayed.

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

 gaGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCg                                                                                        <  2:2635394/92‑1 (MQ=255)

  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       >  2:1321775/1‑93 (MQ=255)

  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       >  1:3890649/1‑93 (MQ=255)

   gAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCggg                                                                                      >  2:4952474/1‑93 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:4669189/93‑1 (MQ=255)

       cGGACTTTGGGAGTTGAGTTCGCGAATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAg                                                                                  <  1:4542227/93‑1 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                >  2:6586289/1‑93 (MQ=255)

             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            <  1:2552543/93‑1 (MQ=255)

               tGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCa                                                                          <  1:1937191/93‑1 (MQ=255)

                ggAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAg                                                                         <  1:7314304/93‑1 (MQ=255)

                ggAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAg                                                                         >  1:2763995/1‑93 (MQ=255)

                 gAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgc                                                                        >  2:6155873/1‑93 (MQ=255)

                 gAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgc                                                                        <  2:7262401/93‑1 (MQ=255)

                   gttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgcgc                                                                      >  1:4297051/1‑93 (MQ=255)

                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCtt                                                                    >  2:4258099/1‑93 (MQ=255)

                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCtt                                                                    >  2:4077565/1‑93 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:3531359/93‑1 (MQ=255)

                        gttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCgg                                                                 >  2:2783592/1‑93 (MQ=255)

                        gttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCgg                                                                 <  2:5049572/93‑1 (MQ=255)

                         ttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGt                                                                >  1:1384076/1‑93 (MQ=255)

                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  1:2317164/93‑1 (MQ=255)

                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  2:4217276/93‑1 (MQ=255)

                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  1:458151/93‑1 (MQ=255)

                            gcgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATg                                                             >  1:7647439/1‑93 (MQ=255)

                             cgaaTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTTTGa                                                            <  2:7747719/90‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:1432103/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:5556902/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            >  2:2380465/1‑93 (MQ=255)

                               gATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcg                                                          <  2:4387744/93‑1 (MQ=255)

                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  1:7117370/1‑93 (MQ=255)

                                  cAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGt                                                       <  1:153447/93‑1 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     <  2:7796066/93‑1 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  1:2174089/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  1:3743/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGGGc                                                     >  1:7186013/1‑93 (MQ=255)

                                     gAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGct                                                    >  2:3078547/1‑93 (MQ=255)

                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  2:371579/93‑1 (MQ=255)

                                       aCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctct                                                  <  1:5894903/93‑1 (MQ=255)

                                         gCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGGGTGCTCTcc                                                >  2:4800632/1‑93 (MQ=255)

                                         gCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTcc                                                >  1:6797636/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              <  1:4999888/93‑1 (MQ=255)

                                            tttttGTCCGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             <  2:6553010/91‑1 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTgg                                             >  2:681780/1‑92 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             >  1:3032677/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             >  1:1990505/1‑93 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            <  2:1610141/93‑1 (MQ=255)

                                              tttGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACg                                           <  1:2882561/93‑1 (MQ=255)

                                               ttGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACgg                                          >  2:4583855/1‑93 (MQ=255)

                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         <  2:7110922/93‑1 (MQ=255)

                                                  tCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGata                                       >  2:2294674/1‑93 (MQ=255)

                                                  tCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGata                                       <  2:1872264/93‑1 (MQ=255)

                                                  tCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGGATGACGCGTGCTCTCCTGACGGata                                       >  2:897770/1‑93 (MQ=255)

                                                   cGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGatat                                      >  1:6121094/1‑93 (MQ=255)

                                                    ggCCTCTGCCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATc                                     <  2:3474047/93‑1 (MQ=255)

                                                    ggCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATc                                     >  1:6171621/1‑93 (MQ=255)

                                                      ccTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCaa                                   >  1:5094857/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  2:5835167/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:1651750/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:7883619/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGAATGACGCGTGCTCTCCTTACGGATATCAATa                                 >  1:5675816/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCAGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:4017150/93‑1 (MQ=255)

                                                        cctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:7454196/92‑1 (MQ=255)

                                                         ctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAc                                <  1:995789/93‑1 (MQ=255)

                                                          tGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACg                               <  2:5287156/93‑1 (MQ=255)

                                                          tGACCAGCTTTCGTAATCGCCGATACCGAGGTGGCGGGTCAGCTCACCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACg                               <  1:7011060/93‑1 (MQ=255)

                                                           gACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACgg                              >  1:1059858/1‑93 (MQ=255)

                                                             ccAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAc                            >  2:5617759/1‑93 (MQ=255)

                                                             ccAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAc                            <  2:2905753/93‑1 (MQ=255)

                                                             ccAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAc                            >  1:3463997/1‑93 (MQ=255)

                                                              cAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAcc                           <  2:1121059/93‑1 (MQ=255)

                                                               aGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCa                          <  1:5607699/93‑1 (MQ=255)

                                                                gCTTTCGTAGTCGCCGATACCGAGGTGGCTGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAg                         <  1:6929344/93‑1 (MQ=255)

                                                                gCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAg                         <  1:5548206/93‑1 (MQ=255)

                                                                  tttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCg                       >  1:939281/1‑93 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  2:6469951/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  1:1149902/93‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  1:6490638/93‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  1:6325953/93‑1 (MQ=255)

                                                                      gTAGTCGCGGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   <  2:7432619/93‑1 (MQ=255)

                                                                      gTAGTCGCCTATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   <  1:2719716/93‑1 (MQ=255)

                                                                      gTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   >  1:1329088/1‑93 (MQ=255)

                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCCATACGGACCAGCGGTACg                  >  2:7563061/1‑93 (MQ=255)

                                                                          tCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCg               >  1:819955/1‑93 (MQ=255)

                                                                            gCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaa             >  2:764980/1‑93 (MQ=255)

                                                                             ccGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaaa            >  2:2471246/1‑93 (MQ=255)

                                                                             ccGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaaa            >  1:6048898/1‑93 (MQ=255)

                                                                              cGATTCCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAc           <  1:5487156/93‑1 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        >  2:3237058/1‑93 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  2:2676242/93‑1 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  2:7909432/93‑1 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        >  1:4889750/1‑93 (MQ=255)

                                                                                  aCCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAttt       <  2:223001/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  1:3122586/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  1:524110/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  2:6033285/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  2:1792160/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGGATGACGCGTGCTCTCCTGACGGCTATCAATCCGGACCAGCGGTACGCCGAAACCTTTc      >  2:6347935/1‑93 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAc    <  1:4190271/93‑1 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAc    <  2:3625821/93‑1 (MQ=255)

                                                                                      gggTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   <  1:4714512/92‑1 (MQ=255)

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`