BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,853	G→A	V178I (GTT→ATT)	nusG →	NusG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,853	0	G	A	100.0%	1225.2 / NA	337	V178I (GTT→ATT)	nusG	NusG
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (171/166); total (171/166)

Only 100 of 342 total aligned reads displayed.

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

gttgAAGAAGTGGATTACGAGAATTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAtt                                                                                          <  2:5391966/93‑1 (MQ=255)

gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAtt                                                                                          >  2:617860/1‑93 (MQ=255)

 ttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTg                                                                                         <  1:2112599/93‑1 (MQ=255)

    aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaa                                                                                      >  1:6839637/1‑93 (MQ=255)

     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGGCCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                     <  1:1598841/93‑1 (MQ=255)

     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                     <  1:7554117/93‑1 (MQ=255)

      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                    >  2:557494/1‑93 (MQ=255)

       aagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAg                                                                                   <  1:6412073/93‑1 (MQ=255)

        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                  >  2:6627964/1‑93 (MQ=255)

         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                 >  1:7875933/1‑93 (MQ=255)

         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                 >  2:2619845/1‑93 (MQ=255)

         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                 <  1:4241812/93‑1 (MQ=255)

          tGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCt                                                                                <  1:7109161/93‑1 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                              <  1:1450842/93‑1 (MQ=255)

            gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTaa                                                                              >  2:5843110/1‑93 (MQ=255)

               tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAccc                                                                           <  1:1037481/93‑1 (MQ=255)

                aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCa                                                                          <  2:3440845/93‑1 (MQ=255)

                 cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAg                                                                         >  2:1282603/1‑93 (MQ=255)

                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCCGATTGAAAAAGCCTAAACCAGc                                                                        >  1:4340046/1‑93 (MQ=255)

                   agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCg                                                                       >  1:5682852/1‑93 (MQ=255)

                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                      >  1:723732/1‑93 (MQ=255)

                     aaaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAt                                                                     <  2:2340629/93‑1 (MQ=255)

                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑a                                                                  >  1:3425585/1‑93 (MQ=255)

                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑a                                                                  >  2:4983014/1‑93 (MQ=255)

                          tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑aaaa                                                               >  2:266583/1‑93 (MQ=255)

                           cGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑aaaaa                                                              >  1:3893481/1‑93 (MQ=255)

                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑aaaaaa                                                             >  1:5857609/1‑93 (MQ=255)

                            ctCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑aaaaaa                                                             <  2:3596718/92‑1 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAg                                                            >  2:1107508/1‑93 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAg                                                            <  1:5063186/93‑1 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAg                                                            >  1:5085825/1‑93 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAg                                                            >  1:3854270/1‑93 (MQ=255)

                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAg                                                            >  1:304620/1‑93 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgc                                                           >  2:1402849/1‑93 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgc                                                           >  2:5221856/1‑93 (MQ=255)

                              cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgc                                                           >  1:2647251/1‑93 (MQ=255)

                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcg                                                          >  1:3737290/1‑93 (MQ=255)

                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcgg                                                         <  1:1942316/93‑1 (MQ=255)

                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcgg                                                         >  2:2222855/1‑93 (MQ=255)

                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcggc                                                        <  2:461938/93‑1 (MQ=255)

                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcggc                                                        <  1:4590321/93‑1 (MQ=255)

                                  aaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcggcg                                                       >  2:7780777/1‑93 (MQ=255)

                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAgcggcgt                                                      >  2:5448399/1‑92 (MQ=255)

                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGa                                                      >  1:141796/1‑93 (MQ=255)

                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGAtt                                                    >  2:7146494/1‑93 (MQ=255)

                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGAtt                                                    <  2:689847/93‑1 (MQ=255)

                                      gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGAttt                                                   >  1:3295706/1‑93 (MQ=255)

                                      gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGAttt                                                   <  1:3794013/93‑1 (MQ=255)

                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAAt                                                <  2:4206446/93‑1 (MQ=255)

                                         tGTTTCTATCATCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAAt                                                <  1:1111659/93‑1 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATc                                               <  1:7706495/93‑1 (MQ=255)

                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATc                                               <  1:5955945/93‑1 (MQ=255)

                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCNGNNATC‑AAAAAAGCGGCGATTTAATCg                                              >  2:3246785/1‑93 (MQ=255)

                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCg                                              <  1:4846972/93‑1 (MQ=255)

                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGt                                             >  1:3603703/1‑93 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGtt                                            <  2:2158343/93‑1 (MQ=255)

                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGtt                                            <  2:4120941/93‑1 (MQ=255)

                                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTg                                           >  1:5468978/1‑93 (MQ=255)

                                               tATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGc                                          >  1:2180310/1‑93 (MQ=255)

                                               tATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGc                                          >  2:6401865/1‑93 (MQ=255)

                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGcac                                        >  1:7604075/1‑93 (MQ=255)

                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGcaca                                       <  2:305171/93‑1 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑ATAAAAGCGGCGATTTAATCGTTGcacag                                      >  1:6712034/1‑92 (MQ=255)

                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACaa                                      >  2:1190196/1‑93 (MQ=255)

                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  2:4486786/93‑1 (MQ=255)

                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  2:2523477/1‑93 (MQ=255)

                                                        tCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                 <  2:1860937/93‑1 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  1:1196547/93‑1 (MQ=255)

                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  2:2779842/93‑1 (MQ=255)

                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  1:1727587/93‑1 (MQ=255)

                                                            gCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgaga                             <  2:3744990/93‑1 (MQ=255)

                                                              gACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                           >  1:3278083/1‑93 (MQ=255)

                                                              gACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                           <  2:2180142/93‑1 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  2:7495718/1‑93 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          <  1:5629659/93‑1 (MQ=255)

                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          >  2:4864505/1‑93 (MQ=255)

                                                                ccccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                         >  2:1736664/1‑93 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:7614152/93‑1 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:1476568/93‑1 (MQ=255)

                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       >  2:6002179/1‑93 (MQ=255)

                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  1:2424395/93‑1 (MQ=255)

                                                                     gTAGAGCTGTACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    <  1:6650543/93‑1 (MQ=255)

                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  <  2:193910/93‑1 (MQ=255)

                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  >  2:204765/1‑93 (MQ=255)

                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  >  1:5574024/1‑93 (MQ=255)

                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  <  1:3246786/93‑1 (MQ=255)

                                                                         agCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                <  1:4692607/93‑1 (MQ=255)

                                                                          gCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt               >  2:1795544/1‑93 (MQ=255)

                                                                          gCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt               <  2:6923768/93‑1 (MQ=255)

                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGGTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              >  1:6218005/1‑93 (MQ=255)

                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              <  1:7299741/93‑1 (MQ=255)

                                                                            tGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAACGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg             <  2:4562258/93‑1 (MQ=255)

                                                                                cTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATATCGCGcc         >  2:4021134/1‑93 (MQ=255)

                                                                                 ttCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCt        >  1:6539965/1‑93 (MQ=255)

                                                                                 ttCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGAGTGAGATTGGAATACAATTTCGCGCCt        >  2:4809304/1‑93 (MQ=255)

                                                                                  tCAGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt       >  1:6991126/1‑93 (MQ=255)

                                                                                    aGCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtttt     <  2:7875932/93‑1 (MQ=255)

                                                                                     gCCAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTg    >  1:5341828/1‑93 (MQ=255)

                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   <  1:6305056/93‑1 (MQ=255)

                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   <  1:816646/93‑1 (MQ=255)

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATC‑AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`