Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,205,033 T→C A26A (GCA→GCG)  purH ← phosphoribosylaminoimidazolecarboxamideformyltra nsferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,205,0330TC99.7% 1041.4 / ‑5.1 329A26A (GCA→GCG) purHphosphoribosylaminoimidazolecarboxamideformyltra nsferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (161/167);  total (161/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01

Only 100 of 335 total aligned reads displayed.

CGGAAACTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGTGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTAAATCCCCT  >  NC_000913/4204945‑4205122
                                                                                        |                                                                                         
 ggAAACTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTg                                                                                      <  2:63857/93‑1 (MQ=255)
 ggAAACTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTg                                                                                      <  2:1246458/93‑1 (MQ=255)
 ggAAACTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTg                                                                                      >  1:23034/1‑93 (MQ=255)
 ggAAACTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTg                                                                                      <  1:6701874/93‑1 (MQ=255)
    aaCTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGc                                                                                   <  1:4838057/93‑1 (MQ=255)
    aaCTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGc                                                                                   >  1:2775636/1‑93 (MQ=255)
      cTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGa                                                                                 >  2:7637598/1‑93 (MQ=255)
      cTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGa                                                                                 >  2:608212/1‑93 (MQ=255)
       ttCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGaa                                                                                >  2:4856175/1‑93 (MQ=255)
        tCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAAt                                                                               >  2:4773616/1‑93 (MQ=255)
         cGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAAtt                                                                              <  2:4613070/93‑1 (MQ=255)
            ttACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTcga                                                                           <  2:7270680/93‑1 (MQ=255)
            ttACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTcga                                                                           <  2:7732476/93‑1 (MQ=255)
             tACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGACCCCTGTAGACAGCAGCTCCACACCGCCTGCGGAAAGCGCCTGTGCCAATTcgac                                                                          >  1:6023040/1‑93 (MQ=255)
               ccGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTcgacga                                                                        <  2:5820118/93‑1 (MQ=255)
               ccGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTcgacga                                                                        <  1:5211479/93‑1 (MQ=255)
               ccGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTcgacga                                                                        <  2:5217455/93‑1 (MQ=255)
                 ggCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATa                                                                      >  1:7706611/1‑93 (MQ=255)
                  gCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATAc                                                                     <  1:6329478/93‑1 (MQ=255)
                  gCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATAc                                                                     <  1:2165041/93‑1 (MQ=255)
                   cAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATAcc                                                                    >  1:3385306/1‑93 (MQ=255)
                    aGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCg                                                                   >  1:107365/1‑93 (MQ=255)
                     gACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCgg                                                                  >  1:7529547/1‑93 (MQ=255)
                       ccTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCt                                                                >  1:853697/1‑93 (MQ=255)
                         tttttCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCttt                                                              <  1:3984989/93‑1 (MQ=255)
                         tttttCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGGGCGGAAAGCGCCTCGGCGAATTCGACGATACCGCCttt                                                              >  2:547651/1‑93 (MQ=255)
                               tGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGa                                                        >  2:4942908/1‑93 (MQ=255)
                               tGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGa                                                        >  2:6989651/1‑93 (MQ=255)
                                gCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGaa                                                       >  1:7352479/1‑93 (MQ=255)
                                gCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGaa                                                       >  1:3074972/1‑93 (MQ=255)
                                gCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGaa                                                       <  1:927570/93‑1 (MQ=255)
                                  tAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAac                                                     >  2:708314/1‑93 (MQ=255)
                                  tAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAac                                                     >  1:6886291/1‑93 (MQ=255)
                                   aaCAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAaca                                                    >  2:601234/1‑93 (MQ=255)
                                      aGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTg                                                 <  2:1770309/93‑1 (MQ=255)
                                      aGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTg                                                 >  2:5305747/1‑93 (MQ=255)
                                      aGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTg                                                 >  1:3677573/1‑93 (MQ=255)
                                       gACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGa                                                >  1:5352996/1‑93 (MQ=255)
                                       gACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGa                                                >  1:1434534/1‑93 (MQ=255)
                                       gACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGa                                                <  2:2132727/93‑1 (MQ=255)
                                       gACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGa                                                >  1:4870139/1‑93 (MQ=255)
                                        aCGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAg                                               <  1:7675849/93‑1 (MQ=255)
                                           ggCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCag                                            <  2:1680239/93‑1 (MQ=255)
                                           ggCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCag                                            <  2:2974562/93‑1 (MQ=255)
                                           ggCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCag                                            <  2:1417601/93‑1 (MQ=255)
                                           ggCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCag                                            <  1:1540657/93‑1 (MQ=255)
                                            gCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCaga                                           <  1:6775119/93‑1 (MQ=255)
                                            gCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCaga                                           <  2:3696476/93‑1 (MQ=255)
                                            gCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCaga                                           <  2:4068407/93‑1 (MQ=255)
                                            gCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCaga                                           <  1:4734035/93‑1 (MQ=255)
                                            gCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCaga                                           <  2:5892669/93‑1 (MQ=255)
                                            gCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCaga                                           <  1:1513309/93‑1 (MQ=255)
                                               gTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAgcg                                        >  1:4163677/1‑93 (MQ=255)
                                               gTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAgcg                                        >  2:4157765/1‑93 (MQ=255)
                                               gTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAgcg                                        >  2:2764022/1‑93 (MQ=255)
                                                tGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAgcgc                                       >  1:6164051/1‑93 (MQ=255)
                                                  cccccTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCgcgg                                     <  2:79538/93‑1 (MQ=255)
                                                   ccccTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCgcggc                                    <  1:5823680/93‑1 (MQ=255)
                                                   ccccTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCgcggc                                    <  2:289298/93‑1 (MQ=255)
                                                    cccTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCgcggcg                                   <  1:2014185/93‑1 (MQ=255)
                                                    cccTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCgcggcg                                   <  1:5602177/93‑1 (MQ=255)
                                                     ccTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGcggcgg                                  >  1:5557302/1‑93 (MQ=255)
                                                      cTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGa                                 >  2:4496989/1‑93 (MQ=255)
                                                      cTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGa                                 <  2:3212506/93‑1 (MQ=255)
                                                      cTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGa                                 >  2:157956/1‑93 (MQ=255)
                                                        gTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACt                               >  2:3109062/1‑93 (MQ=255)
                                                        gTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACt                               >  2:3551485/1‑93 (MQ=255)
                                                        gTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACt                               >  2:676527/1‑93 (MQ=255)
                                                        gTAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACt                               >  1:1484577/1‑93 (MQ=255)
                                                         tAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTg                              <  2:2534471/93‑1 (MQ=255)
                                                         tAGACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTg                              >  1:6244010/1‑93 (MQ=255)
                                                           gACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGga                            <  1:5150161/93‑1 (MQ=255)
                                                           gACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGga                            >  2:6224617/1‑93 (MQ=255)
                                                           gACAGCAGCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGga                            <  1:705563/93‑1 (MQ=255)
                                                             cagcagCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGgacg                          >  1:6470141/1‑93 (MQ=255)
                                                             cagcagCTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGgacg                          >  2:2234458/1‑93 (MQ=255)
                                                              agcagcTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGgacga                         >  1:4292285/1‑93 (MQ=255)
                                                                 agcTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACgt                      <  1:2214429/93‑1 (MQ=255)
                                                                  gcTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACgtt                     >  2:6605792/1‑93 (MQ=255)
                                                                  gcTCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACgtt                     >  1:1857067/1‑93 (MQ=255)
                                                                    tCCACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACgttgt                   >  2:4279516/1‑93 (MQ=255)
                                                                     ccACACCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACgttgtt                  <  2:2418033/93‑1 (MQ=255)
                                                                        cacCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCa               <  2:6885575/93‑1 (MQ=255)
                                                                        cacCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCa               <  2:3957247/93‑1 (MQ=255)
                                                                         cccGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCAt              <  2:5680240/92‑1 (MQ=255)
                                                                         acCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCAt              >  2:922057/1‑93 (MQ=255)
                                                                         acCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCAt              <  1:3239410/93‑1 (MQ=255)
                                                                         acCGCGTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCAt              <  2:5058403/93‑1 (MQ=255)
                                                                           cgcgTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATgg            >  1:2647516/1‑93 (MQ=255)
                                                                            gcgTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGt           <  2:1031238/93‑1 (MQ=255)
                                                                            ccgTGCGGAAATCGCCGTGGCNANTTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGt           <  1:1656937/92‑1 (MQ=255)
                                                                             cgTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTa          >  2:2004155/1‑93 (MQ=255)
                                                                              gTGCGGAAAGCGCCTGGGTGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTaa         <  1:1031276/93‑1 (MQ=255)
                                                                              gTGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTaa         >  2:1865510/1‑93 (MQ=255)
                                                                               tGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTaaa        >  1:5899300/1‑93 (MQ=255)
                                                                               tGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTaaa        <  1:4497389/93‑1 (MQ=255)
                                                                               tGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTaaa        <  1:1717007/93‑1 (MQ=255)
                                                                               tGCGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTaaa        >  2:7514731/1‑93 (MQ=255)
                                                                                 cGGAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTAAATc      <  2:3367926/93‑1 (MQ=255)
                                                                                   gAAAGCGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTAAATccc    >  2:4548285/1‑93 (MQ=255)
                                                                                        |                                                                                         
CGGAAACTTCGGTTACCGGCAGACCTTTTTCTGCTAACAGACGGGCAGTGCCCCCTGTAGACAGCAGCTCCACACCGCGTGCGGAAAGTGCCTGGGCGAATTCGACGATACCGGCTTTGTCAGAAACACTGAGCAGAGCGCGGCGGACTGGACGACGTTGTTGCATGGTAAATCCCCT  >  NC_000913/4204945‑4205122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑