BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,214,346	A→G	E430E (GAA→GAG)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,214,346	0	A	G	99.2%	1173.9 / ‑2.7	376	E430E (GAA→GAG)	aceB	malate synthase A
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (182/191); total (183/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 381 total aligned reads displayed.

TTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA  >  NC_000913/4214255‑4214435

  aCTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCg                                                                                        >  1:6578063/1‑93 (MQ=255)

    tGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTg                                                                                      >  2:1063244/1‑93 (MQ=255)

         aTCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtct                                                                                 >  2:2134481/1‑93 (MQ=255)

         aTCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtct                                                                                 >  1:6651008/1‑93 (MQ=255)

         aTCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtct                                                                                 >  1:2695707/1‑93 (MQ=255)

          tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                                >  2:5293256/1‑93 (MQ=255)

          tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                                <  1:1894248/93‑1 (MQ=255)

               tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                           >  1:1683978/1‑93 (MQ=255)

                gctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCaa                                                                          <  1:1986473/93‑1 (MQ=255)

                 ctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATAGAGGCGTGGATCTCTGGCAAc                                                                         <  1:1402731/93‑1 (MQ=255)

                   ggCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACgg                                                                       >  2:4257980/1‑93 (MQ=255)

                     cACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCt                                                                     <  1:4614429/93‑1 (MQ=255)

                     cACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCt                                                                     <  2:4226785/93‑1 (MQ=255)

                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTCCATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  >  2:4998515/1‑93 (MQ=255)

                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGTATCTCTGGCAACGGCtgtg                                                                  <  1:1439171/93‑1 (MQ=255)

                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  >  1:4023329/1‑93 (MQ=255)

                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  <  1:5096375/93‑1 (MQ=255)

                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  <  2:6757671/93‑1 (MQ=255)

                         ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgt                                                                 >  1:3300284/1‑93 (MQ=255)

                         ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgt                                                                 <  1:7870137/93‑1 (MQ=255)

                         ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgt                                                                 <  2:728587/93‑1 (MQ=255)

                           gtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGc                                                               >  1:1284238/1‑93 (MQ=255)

                           gtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGc                                                               <  1:5696514/93‑1 (MQ=255)

                           gtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGc                                                               <  1:5485105/93‑1 (MQ=255)

                            tgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGcc                                                              >  2:1236211/1‑93 (MQ=255)

                            tgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGcc                                                              >  1:3935303/1‑93 (MQ=255)

                            tgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGcc                                                              >  2:2711730/1‑93 (MQ=255)

                              aTGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTGCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGa                                                            <  1:6857372/93‑1 (MQ=255)

                                ggTGAACGCACCGAAGAAGGTATGCGCGCCAACTTTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAtt                                                          <  1:7605809/93‑1 (MQ=255)

                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  2:4630608/93‑1 (MQ=255)

                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        >  1:5667512/1‑93 (MQ=255)

                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:1031530/93‑1 (MQ=255)

                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:3034543/93‑1 (MQ=255)

                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:4827184/93‑1 (MQ=255)

                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:36280/93‑1 (MQ=255)

                                   gAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAt                                                       <  2:7402305/93‑1 (MQ=255)

                                   gAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAt                                                       <  2:7181773/93‑1 (MQ=255)

                                    aaCGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATg                                                      <  1:4408868/93‑1 (MQ=255)

                                      cGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGc                                                    <  1:6005228/93‑1 (MQ=255)

                                      cGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGc                                                    >  2:6084889/1‑93 (MQ=255)

                                       gCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTTTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGcc                                                   >  1:5011100/1‑93 (MQ=255)

                                         aCCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTg                                                 <  1:7476743/93‑1 (MQ=255)

                                          ccGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGa                                                <  2:1318998/93‑1 (MQ=255)

                                           cGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGAt                                               <  1:4538112/93‑1 (MQ=255)

                                             aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                             <  2:6733088/93‑1 (MQ=255)

                                              agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGa                                            >  2:2967799/1‑93 (MQ=255)

                                              agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGa                                            <  2:5103860/93‑1 (MQ=255)

                                               gaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGaa                                           <  1:6942856/93‑1 (MQ=255)

                                                aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAg                                          <  1:4229752/93‑1 (MQ=255)

                                                aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAg                                          <  2:388527/93‑1 (MQ=255)

                                                aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAg                                          <  1:371654/93‑1 (MQ=255)

                                                 agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACCTCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAATTATGGCCTGATGGAAGa                                         <  2:7680201/93‑1 (MQ=255)

                                                  ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                        >  1:712100/1‑93 (MQ=255)

                                                  ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                        <  2:6694850/93‑1 (MQ=255)

                                                  ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                        <  2:2347934/93‑1 (MQ=255)

                                                   gTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATg                                       <  1:7057182/93‑1 (MQ=255)

                                                    tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                      <  1:5846374/93‑1 (MQ=255)

                                                    tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                      <  1:5271551/93‑1 (MQ=255)

                                                     aTGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg                                     >  2:7114477/1‑93 (MQ=255)

                                                     aTGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgag                                     >  1:6289382/1‑91 (MQ=255)

                                                     aTGCGCGCCAACATGCGGGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg                                     <  1:4084384/93‑1 (MQ=255)

                                                      tGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcgg                                    >  1:6486545/1‑93 (MQ=255)

                                                         gcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa                                 <  1:6777978/93‑1 (MQ=255)

                                                          cgcCAGCATTCCCGTGGCTGTGCAGTACAGCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                                <  1:7364713/93‑1 (MQ=255)

                                                          cgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                                <  1:157472/93‑1 (MQ=255)

                                                           gcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACg                               >  1:2461115/1‑93 (MQ=255)

                                                           gcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACg                               <  1:3188546/93‑1 (MQ=255)

                                                            ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGGGACgg                              >  2:5908872/1‑93 (MQ=255)

                                                            ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACgg                              >  2:4005037/1‑93 (MQ=255)

                                                            ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACgg                              <  2:6504640/93‑1 (MQ=255)

                                                             cAACATTCGCGTGGCTGTGCAGTACATCGGGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGc                             >  2:4774951/1‑93 (MQ=255)

                                                              aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                            >  2:2527805/1‑93 (MQ=255)

                                                              aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                            <  2:1601555/93‑1 (MQ=255)

                                                              aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                            >  1:3467769/1‑93 (MQ=255)

                                                               aCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTg                           <  1:2836978/93‑1 (MQ=255)

                                                                  ttCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGaaa                        >  2:6375662/1‑93 (MQ=255)

                                                                   tCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAt                       <  2:2837372/93‑1 (MQ=255)

                                                                     gcgTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAttt                     >  1:7202129/1‑93 (MQ=255)

                                                                       gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                   >  2:181061/1‑93 (MQ=255)

                                                                        tGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTccc                  >  2:7487034/1‑93 (MQ=255)

                                                                          gCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGt                >  1:2337697/1‑93 (MQ=255)

                                                                          gCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGt                >  1:2095928/1‑93 (MQ=255)

                                                                           cTGTGCAGTACATCGAGGCGTGGACCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGACATTTCCCGTa               <  2:2472464/93‑1 (MQ=255)

                                                                            tgtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAc              >  2:849298/1‑93 (MQ=255)

                                                                            tgtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAc              >  2:5352613/1‑93 (MQ=255)

                                                                            tgtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAc              >  2:436417/1‑93 (MQ=255)

                                                                             gtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc             <  1:4958689/93‑1 (MQ=255)

                                                                              tgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGGGGCGACGGCTGAAATTTCCCGTACCt            >  1:6143924/1‑93 (MQ=255)

                                                                              tgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCt            <  2:3509282/93‑1 (MQ=255)

                                                                               gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc           <  2:6179568/93‑1 (MQ=255)

                                                                               gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc           >  2:3328803/1‑93 (MQ=255)

                                                                               gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc           <  2:7620240/93‑1 (MQ=255)

                                                                                 aGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGa         >  1:87419/1‑93 (MQ=255)

                                                                                    aCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt      <  2:3573715/93‑1 (MQ=255)

                                                                                      aTCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTgg    <  2:70105/93‑1 (MQ=255)

                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTTGc   >  1:1173759/1‑93 (MQ=255)

                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   >  2:4968006/1‑93 (MQ=255)

                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   >  2:1927600/1‑93 (MQ=255)

                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   >  1:7776913/1‑93 (MQ=255)

                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   <  1:4684180/93‑1 (MQ=255)

TTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA  >  NC_000913/4214255‑4214435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`