BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,945	T→C	S420P (TCT→CCT)	aceA →	isocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,945	0	T	C	99.7%	1246.2 / ‑5.1	392	S420P (TCT→CCT)	aceA	isocitrate lyase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (211/180); total (212/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 397 total aligned reads displayed.

GCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCA  >  NC_000913/4215855‑4216035

gcgAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCt                                                                                          >  2:4508333/1‑93 (MQ=255)

gcgAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCt                                                                                          <  2:5636046/93‑1 (MQ=255)

  gAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTCCAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTc                                                                                        <  1:214970/93‑1 (MQ=255)

  gAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTc                                                                                        <  1:1773588/93‑1 (MQ=255)

   aaaGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCa                                                                                       <  2:4307043/93‑1 (MQ=255)

    aaGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACCGGTTACTTCGATAAAGTGACGCCTATTATTCAGGGCGGCACGCCTTCAg                                                                                      <  2:2781393/93‑1 (MQ=255)

    aaGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAg                                                                                      >  2:3573117/1‑93 (MQ=255)

    aaGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAg                                                                                      <  1:3786316/93‑1 (MQ=255)

      gATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTc                                                                                    >  2:4054150/1‑93 (MQ=255)

      gATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTc                                                                                    <  2:2432109/93‑1 (MQ=255)

      gATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTc                                                                                    <  2:7527842/93‑1 (MQ=255)

       aTGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCa                                                                                   <  2:6692955/93‑1 (MQ=255)

         ggCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCAcc                                                                                 <  2:4897661/93‑1 (MQ=255)

          gCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACcg                                                                                >  2:1791084/1‑93 (MQ=255)

          gCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACcg                                                                                >  1:330435/1‑93 (MQ=255)

           cTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACcgc                                                                               <  1:6210401/93‑1 (MQ=255)

            tataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACcgcg                                                                              >  1:4998615/1‑93 (MQ=255)

            tataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACcgcg                                                                              >  1:487979/1‑93 (MQ=255)

            tataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGGTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACcgcg                                                                              >  1:1895668/1‑93 (MQ=255)

             ataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGTCGGCACGCCTTCAGTCACCgcgc                                                                             <  2:2019650/93‑1 (MQ=255)

             ataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCgcgc                                                                             >  1:2415873/1‑93 (MQ=255)

             ataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCgcgc                                                                             <  1:6338499/93‑1 (MQ=255)

             ataCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCgcgc                                                                             <  1:2816604/93‑1 (MQ=255)

             ataCCTTCGTATCTCACCAGCAGGAAGTGGGCACAGGTTACTTAGATAAAGTGACGACTATTATTCAGAGCGGCACGCGTTCCGTCACCgcgc                                                                             >  1:3954042/1‑93 (MQ=255)

              taCCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCt                                                                            >  1:1258696/1‑93 (MQ=255)

                ccTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGa                                                                          <  2:6853875/93‑1 (MQ=255)

                 cTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGAc                                                                         >  1:3092636/1‑93 (MQ=255)

                    cGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCgg                                                                      >  1:6522601/1‑93 (MQ=255)

                     gTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGc                                                                     >  2:3945344/1‑93 (MQ=255)

                      tATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCt                                                                    >  2:7208486/1‑93 (MQ=255)

                        tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTcc                                                                  >  1:1059488/1‑93 (MQ=255)

                        tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTcc                                                                  >  2:5034055/1‑93 (MQ=255)

                        tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTcc                                                                  >  2:5480255/1‑93 (MQ=255)

                        tctcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTcc                                                                  >  2:7086508/1‑93 (MQ=255)

                          tcACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCAc                                                                <  2:508689/93‑1 (MQ=255)

                           cACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACt                                                               <  1:2564262/93‑1 (MQ=255)

                            aCCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTg                                                              >  2:4557666/1‑93 (MQ=255)

                            aCCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTg                                                              >  1:348412/1‑93 (MQ=255)

                              cagcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTgaa                                                            <  2:2916861/93‑1 (MQ=255)

                              cagcagGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTgaa                                                            >  2:1671505/1‑93 (MQ=255)

                                  agGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAAt                                                        >  1:5135696/1‑93 (MQ=255)

                                  agGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAAt                                                        <  2:5989231/93‑1 (MQ=255)

                                   ggAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATc                                                       >  2:874285/1‑93 (MQ=255)

                                   ggAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATc                                                       >  2:1503066/1‑93 (MQ=255)

                                   ggAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATc                                                       <  2:1431481/93‑1 (MQ=255)

                                    gAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCg                                                      >  1:5420404/1‑93 (MQ=255)

                                    gAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCg                                                      >  2:2638926/1‑93 (MQ=255)

                                     aaGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGc                                                     <  2:2181486/93‑1 (MQ=255)

                                      aGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCa                                                    <  2:6058299/93‑1 (MQ=255)

                                      aGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCa                                                    <  1:4349515/93‑1 (MQ=255)

                                        tGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGt                                                  <  2:3422495/93‑1 (MQ=255)

                                          ggTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTc                                                >  2:5724864/1‑93 (MQ=255)

                                          ggTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTc                                                <  1:59751/93‑1 (MQ=255)

                                          ggTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTc                                                >  2:2504400/1‑93 (MQ=255)

                                          ggTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTc                                                <  2:7404632/93‑1 (MQ=255)

                                           gTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCt                                               <  1:3176081/93‑1 (MQ=255)

                                            tACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTa                                              >  1:1737906/1‑93 (MQ=255)

                                              cAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAg                                            >  2:4699000/1‑93 (MQ=255)

                                              cAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAg                                            >  2:1253003/1‑93 (MQ=255)

                                               aGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGc                                           >  1:961059/1‑93 (MQ=255)

                                                    aCTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCACAGTTCTAAGcaacaa                                      >  1:6652217/1‑93 (MQ=255)

                                                     cTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaacaac                                     <  1:7610894/93‑1 (MQ=255)

                                                     cTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaacaac                                     <  2:3151165/93‑1 (MQ=255)

                                                     cTTCGATAAAGTGACGACTATTATGCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGcaacaac                                     <  1:854029/93‑1 (MQ=255)

                                                        cGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCaacaacaac                                  <  1:7092567/93‑1 (MQ=255)

                                                        cGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCaacaacaac                                  <  2:7451874/93‑1 (MQ=255)

                                                        cGATAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCaacaacaac                                  <  1:5238022/93‑1 (MQ=255)

                                                        cGAAAAAGTGACCACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCaacaacaac                                  <  1:5749911/93‑1 (MQ=255)

                                                          aTAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCg                                <  2:6573969/93‑1 (MQ=255)

                                                           tAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGt                               >  1:4540152/1‑93 (MQ=255)

                                                           tAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGt                               <  1:4134314/93‑1 (MQ=255)

                                                           tAAAGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGt                               <  1:4738001/93‑1 (MQ=255)

                                                           aaaaaTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGt                               <  1:6064606/92‑1 (MQ=255)

                                                             aaGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTg                             <  2:2110535/93‑1 (MQ=255)

                                                              aGTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGc                            <  2:1575665/93‑1 (MQ=255)

                                                               gTGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCt                           <  2:6463341/93‑1 (MQ=255)

                                                                tGACGACTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTg                          >  2:6302913/1‑93 (MQ=255)

                                                                 gacgacTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGa                         >  2:3295226/1‑93 (MQ=255)

                                                                 gacgacTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGa                         <  1:120005/93‑1 (MQ=255)

                                                                    gacTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTg                      <  2:2345801/93‑1 (MQ=255)

                                                                    gacTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTg                      >  1:2963495/1‑93 (MQ=255)

                                                                     acTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGt                     <  1:7401208/93‑1 (MQ=255)

                                                                      cTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTa                    >  1:7479149/1‑93 (MQ=255)

                                                                      cTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTa                    <  1:3620234/93‑1 (MQ=255)

                                                                      cTATTATTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTa                    <  2:3027506/93‑1 (MQ=255)

                                                                       tattatTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAg                   <  2:5363461/93‑1 (MQ=255)

                                                                       tattatTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAg                   >  1:2862758/1‑93 (MQ=255)

                                                                       tattatTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAg                   <  2:612861/93‑1 (MQ=255)

                                                                       tattatTCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAg                   <  2:3871305/93‑1 (MQ=255)

                                                                        attattCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAgg                  <  1:1535806/93‑1 (MQ=255)

                                                                          tattCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGcc                >  1:1009846/1‑93 (MQ=255)

                                                                           attCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCg               <  1:7827905/93‑1 (MQ=255)

                                                                            ttCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCCGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCgg              >  2:7811766/1‑93 (MQ=255)

                                                                            ttCAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCgg              >  1:2652507/1‑93 (MQ=255)

                                                                             tCAGGTCGGCACGCCTTCAGTCACCGCTCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGa             <  1:1345489/93‑1 (MQ=255)

                                                                              cAGGGCGGCACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGAt            >  2:7277072/1‑93 (MQ=255)

                                                                                   gggcACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGc       <  1:7688678/92‑1 (MQ=255)

                                                                                   cggcACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGc       <  1:7290539/93‑1 (MQ=255)

                                                                                      cACGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGtt    <  1:4253328/93‑1 (MQ=255)

                                                                                       aCGCCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTc   >  1:6823021/1‑93 (MQ=255)

GCGAAAGATGGCTATACCTTCGTATCTCACCAGCAGGAAGTGGGTACAGGTTACTTCGATAAAGTGACGACTATTATTCAGGGCGGCACGTCTTCAGTCACCGCGCTGACCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCA  >  NC_000913/4215855‑4216035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`