BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,239,076	A→G	V253A (GTA→GCA)	xylE ←	xylose‑proton symport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,239,076	0	A	G	99.7%	1110.5 / NA	348	V253A (GTA→GCA)	xylE	xylose‑proton symport
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (193/153); total (194/154)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 360 total aligned reads displayed.

ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGA  >  NC_000913/4238986‑4239165

aTTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCt                                                                                         >  1:6541387/1‑93 (MQ=255)

aTTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCt                                                                                         <  1:3035722/93‑1 (MQ=255)

 ttACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTg                                                                                        >  1:2402933/1‑93 (MQ=255)

    cGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCt                                                                                     <  2:6681804/93‑1 (MQ=255)

     gCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTg                                                                                    >  1:1586824/1‑93 (MQ=255)

       cGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAg                                                                                  <  2:2391085/93‑1 (MQ=255)

       cGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAg                                                                                  >  1:1755124/1‑93 (MQ=255)

        gATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGt                                                                                 >  2:1624204/1‑93 (MQ=255)

        gATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGt                                                                                 <  1:1632682/93‑1 (MQ=255)

        gATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGt                                                                                 <  2:6698640/93‑1 (MQ=255)

         aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGtt                                                                                <  2:4460440/93‑1 (MQ=255)

         aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGtt                                                                                >  2:5684547/1‑93 (MQ=255)

         aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGtt                                                                                <  1:2116483/93‑1 (MQ=255)

          ttACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTg                                                                               >  2:1291034/1‑93 (MQ=255)

           tACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGc                                                                              <  1:257232/93‑1 (MQ=255)

            aCAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCa                                                                             >  1:5280769/1‑93 (MQ=255)

            aCAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCa                                                                             >  2:2576885/1‑93 (MQ=255)

            aCAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCa                                                                             >  2:6604349/1‑93 (MQ=255)

             cAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCaa                                                                            <  2:7171592/93‑1 (MQ=255)

              aaTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAg                                                                           >  1:1387729/1‑93 (MQ=255)

                 cacgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCgt                                                                        >  1:1461356/1‑93 (MQ=255)

                 cacgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCgg                                                                        >  2:2280830/1‑92 (MQ=255)

                  acgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGGGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCgtg                                                                       >  1:4898624/1‑93 (MQ=255)

                     cccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTg                                                                    >  2:1143488/1‑93 (MQ=255)

                      ccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGc                                                                   >  2:2653372/1‑93 (MQ=255)

                       cacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGcc                                                                  >  2:63806/1‑93 (MQ=255)

                        acgccACACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGccc                                                                 >  2:7787084/1‑93 (MQ=255)

                            cAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGAACCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATaa                                                             <  1:4099474/93‑1 (MQ=255)

                              aaCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAtt                                                           >  1:81654/1‑93 (MQ=255)

                              aaCATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAtt                                                           >  1:4417340/1‑93 (MQ=255)

                                cATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGc                                                                          <  1:4966064/76‑1 (MQ=255)

                                cATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAAtttt                                                         <  2:391176/93‑1 (MQ=255)

                                 aTCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                        >  1:4502742/1‑93 (MQ=255)

                                 aTCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                        >  2:220781/1‑93 (MQ=255)

                                 aTCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                        >  1:2296928/1‑93 (MQ=255)

                                 aTCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                        <  2:3516394/93‑1 (MQ=255)

                                  tcggcagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTgc                                                       <  1:3805002/90‑1 (MQ=255)

                                  tCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTgc                                                       >  2:6463136/1‑93 (MQ=255)

                                     gcagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCa                                                    <  1:4233461/93‑1 (MQ=255)

                                      cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   <  2:3097384/93‑1 (MQ=255)

                                      cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   <  1:1139294/93‑1 (MQ=255)

                                      cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   <  2:802330/93‑1 (MQ=255)

                                      cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAg                                                   <  1:5730695/93‑1 (MQ=255)

                                       agACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAgg                                                  >  1:436164/1‑93 (MQ=255)

                                        gacgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGa                                                 >  2:4236649/1‑93 (MQ=255)

                                        gacgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGa                                                 <  1:5805945/93‑1 (MQ=255)

                                         acgacCACCGGTTTTGCGTCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGAt                                                <  2:5262243/93‑1 (MQ=255)

                                         acgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGAt                                                <  1:6149411/93‑1 (MQ=255)

                                         acgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGAt                                                <  2:1530464/93‑1 (MQ=255)

                                          cgacCCCCGGTTTTGCGGCCATGATCCAGGGGGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATa                                               <  1:5446182/93‑1 (MQ=255)

                                           gacCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAc                                              <  1:4160819/93‑1 (MQ=255)

                                            accaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                             <  1:1291154/93‑1 (MQ=255)

                                            accaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                             <  1:3378142/93‑1 (MQ=255)

                                            accaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                             >  1:7055648/1‑93 (MQ=255)

                                            accaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                             >  2:6278796/1‑93 (MQ=255)

                                            accaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                             <  2:5772932/93‑1 (MQ=255)

                                            accaccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                             <  2:3244561/93‑1 (MQ=255)

                                             ccaccGGTTTTGCGTCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCt                                            <  2:4023305/93‑1 (MQ=255)

                                              caccGGTTTGGCGGCCATGATCCAGGGAGGGTTTAATTGCCTGTGCTGCCTGAGGTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATGCCtt                                           <  1:1526087/93‑1 (MQ=255)

                                               accGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTc                                          >  1:7501552/1‑93 (MQ=255)

                                               accGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTc                                          >  1:1002383/1‑93 (MQ=255)

                                                ccGGTTTTGCGGCCATGATCCAGTGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                         <  2:4293993/93‑1 (MQ=255)

                                                ccGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                         <  2:6068085/93‑1 (MQ=255)

                                                   gTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGcc                                      >  1:738696/1‑93 (MQ=255)

                                                   gTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGcc                                      <  2:3450020/93‑1 (MQ=255)

                                                        gCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTc                                 <  2:5876914/93‑1 (MQ=255)

                                                         cGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTct                                <  1:51628/93‑1 (MQ=255)

                                                         cGGCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTct                                <  1:2750802/93‑1 (MQ=255)

                                                          ggCCATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTctt                               <  1:7775370/93‑1 (MQ=255)

                                                             cATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgct                            <  1:97408/93‑1 (MQ=255)

                                                             cATGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgct                            >  2:3783736/1‑93 (MQ=255)

                                                               tGATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgcttg                          <  2:4749406/93‑1 (MQ=255)

                                                                gATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCttgcttgc                         <  1:7872695/93‑1 (MQ=255)

                                                                gATCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCttgcttgc                         <  1:539030/93‑1 (MQ=255)

                                                                  tCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                       >  1:609422/1‑93 (MQ=255)

                                                                  tCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                       >  2:2871412/1‑93 (MQ=255)

                                                                  tCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                       >  2:5505633/1‑93 (MQ=255)

                                                                  tCCAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGATGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                       <  1:3473968/93‑1 (MQ=255)

                                                                    cAGGGAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcg                     >  1:3876359/1‑93 (MQ=255)

                                                                       ggAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcgcgc                  >  1:6894675/1‑92 (MQ=255)

                                                                       ggAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGa                  <  1:5869610/93‑1 (MQ=255)

                                                                       ggAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGa                  >  2:2081150/1‑93 (MQ=255)

                                                                        gAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGAc                 <  1:5957545/93‑1 (MQ=255)

                                                                        gAGTGTTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGAc                 <  1:4681652/93‑1 (MQ=255)

                                                                          gtgtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACAt               >  2:7499971/1‑93 (MQ=255)

                                                                           tgtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATc              <  2:1949576/93‑1 (MQ=255)

                                                                           tgtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATc              <  2:4846831/93‑1 (MQ=255)

                                                                            gtTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGAACGAAATca             <  2:7045440/93‑1 (MQ=255)

                                                                               tAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagcc          >  2:6981838/1‑93 (MQ=255)

                                                                                aaTTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagcca         <  1:2446266/93‑1 (MQ=255)

                                                                                 aTTTCCTGTGCTGGCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCCCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACAtcagtcag        >  2:7726913/1‑93 (MQ=255)

                                                                                 aTTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagccag        <  2:4542775/93‑1 (MQ=255)

                                                                                  tttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagccagc       >  1:6375094/1‑93 (MQ=255)

                                                                                  tttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagccagc       <  1:6605419/93‑1 (MQ=255)

                                                                                   ttCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCg      >  1:4576203/1‑93 (MQ=255)

                                                                                    tCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGa     <  1:3180110/93‑1 (MQ=255)

                                                                                     ccTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAg    >  2:7609852/1‑93 (MQ=255)

                                                                                      cTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAgg   >  1:2835836/1‑93 (MQ=255)

                                                                                       tgtgCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGa  <  2:5111878/93‑1 (MQ=255)

                                                                                       tgtgCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGa  >  2:5010233/1‑93 (MQ=255)

ATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGA  >  NC_000913/4238986‑4239165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`