Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,242,957 C→T E348K (GAA→AAA)  malE ← periplasmic maltose‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,242,9570CT99.5% 1432.1 / ‑6.1 393E348K (GAA→AAA) malEperiplasmic maltose‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (175/216);  total (175/218)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046
                                                                                          |                                                                                         
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         <  2:2450524/93‑1 (MQ=255)
tGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCa                                                                                         >  1:5291183/1‑93 (MQ=255)
 ggcggcGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAt                                                                                        <  1:6680078/93‑1 (MQ=255)
  gcggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtg                                                                                       <  1:7906156/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  1:5189220/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  1:3825215/93‑1 (MQ=255)
    ggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggt                                                                                     <  2:3117613/93‑1 (MQ=255)
       gTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCAtggtggg                                                                                  >  1:195100/1‑92 (MQ=255)
       gTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggc                                                                                  <  1:6143308/93‑1 (MQ=255)
        ttGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTggcg                                                                                 >  1:3258495/1‑93 (MQ=255)
            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             <  2:5649509/93‑1 (MQ=255)
            tCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCaa                                                                             >  1:1472383/1‑93 (MQ=255)
            tCACCAAAGTGCGCAAGGCGTACCAGAAAGCGGACAACTAAGGGCTGTTCGGCATGATTACACCTTTCTGGACGTTTTTCGTGGTGACGGCaa                                                                             <  1:6034683/93‑1 (MQ=255)
              aCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATTATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATa                                                                           >  2:5881689/1‑93 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:4111394/93‑1 (MQ=255)
               ccGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATAc                                                                          <  2:7306648/93‑1 (MQ=255)
                  cAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGGGGCGGCAATACGTg                                                                       >  1:887975/1‑93 (MQ=255)
                   aGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTgg                                                                      >  2:6057274/1‑93 (MQ=255)
                    gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     <  1:4064238/93‑1 (MQ=255)
                    gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     <  1:4756557/93‑1 (MQ=255)
                    gTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGa                                                                     >  1:2402964/1‑93 (MQ=255)
                     tACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAt                                                                    <  2:1250952/93‑1 (MQ=255)
                      aCGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATc                                                                   >  1:25800/1‑93 (MQ=255)
                       cGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCt                                                                  <  2:3959238/93‑1 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACCTCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGGAATACGTGGATCtt                                                                 >  1:6211437/1‑93 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 >  1:6868217/1‑93 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 <  2:6944283/93‑1 (MQ=255)
                        gCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCtt                                                                 <  2:7893510/93‑1 (MQ=255)
                         cACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCttt                                                                <  1:6735818/93‑1 (MQ=255)
                          aCGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTc                                                               <  1:1045649/93‑1 (MQ=255)
                          aCGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTc                                                               <  1:2475842/93‑1 (MQ=255)
                          aCGACATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTc                                                               <  2:2751494/93‑1 (MQ=255)
                             gCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGcc                                                            >  2:5252431/1‑93 (MQ=255)
                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAc                                                         >  2:1940219/1‑93 (MQ=255)
                                tACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAAc                                                         >  2:4835245/1‑93 (MQ=255)
                                  ccAGAAAGCGGCCATCTGGGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGAGCTTTCGCCAGctc                                                       <  2:1558410/93‑1 (MQ=255)
                                  ccAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAActc                                                       <  1:6515536/93‑1 (MQ=255)
                                    aGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCtt                                                     >  2:5570701/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  1:4580394/1‑93 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    <  2:2537974/93‑1 (MQ=255)
                                     gAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                    >  2:2925511/1‑93 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  2:100625/93‑1 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  2:6816552/93‑1 (MQ=255)
                                      aaaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTcc                                                   <  2:7812999/93‑1 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  1:4189691/93‑1 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  1:779795/93‑1 (MQ=255)
                                       aaGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  2:338884/93‑1 (MQ=255)
                                       aaGCGGACATCGGCGGGATGTTCGGCATGATTGCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCt                                                  <  2:1715384/93‑1 (MQ=255)
                                        aGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTc                                                 <  1:1737782/93‑1 (MQ=255)
                                        aGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTc                                                 <  2:3465183/93‑1 (MQ=255)
                                          cGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                               <  2:3322034/93‑1 (MQ=255)
                                           ggACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTa                                              >  2:1821116/1‑93 (MQ=255)
                                            gACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaa                                             <  2:4502454/93‑1 (MQ=255)
                                            gACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTaa                                             <  1:5556317/93‑1 (MQ=255)
                                             aCATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAg                                            <  2:7933400/93‑1 (MQ=255)
                                             aCATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAg                                            >  1:2232767/1‑93 (MQ=255)
                                              cATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAgc                                           >  1:7597679/1‑92 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         >  2:3082734/1‑93 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         >  2:5072254/1‑93 (MQ=255)
                                                tCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                         <  2:3880802/93‑1 (MQ=255)
                                                  tGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTc                                       <  1:2293401/93‑1 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      >  1:1797253/1‑93 (MQ=255)
                                                   gCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCa                                      <  2:7764440/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     <  2:1035474/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     <  1:727112/93‑1 (MQ=255)
                                                    cGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAg                                     >  1:5726228/1‑93 (MQ=255)
                                                       gATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAgcgc                                  >  2:5391497/1‑93 (MQ=255)
                                                         tGTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTa                                <  1:1221938/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               <  2:4758419/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               <  2:5928663/93‑1 (MQ=255)
                                                          gTTCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTAc                               <  2:3505326/93‑1 (MQ=255)
                                                            tCGGCATGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACgg                             >  1:1850499/1‑93 (MQ=255)
                                                                cATGATTTCACATTTCTGGGTGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAcc                         <  2:1405885/93‑1 (MQ=255)
                                                                 aTGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCAccc                        >  1:3522370/1‑93 (MQ=255)
                                                                  tGATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCa                       >  2:2277196/1‑93 (MQ=255)
                                                                   gATTTCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAg                      >  2:6206691/1‑93 (MQ=255)
                                                                     tttCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCg                    <  2:7363202/93‑1 (MQ=255)
                                                                       tccccTTTCTGGGCCTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:6778641/90‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  >  1:3452571/1‑93 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:144438/93‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:5795286/93‑1 (MQ=255)
                                                                       tCACCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGt                  <  2:5983206/93‑1 (MQ=255)
                                                                         tccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  2:704898/92‑1 (MQ=255)
                                                                         cccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  2:7233811/92‑1 (MQ=255)
                                                                         cccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  1:6372375/92‑1 (MQ=255)
                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                >  2:7463988/1‑93 (MQ=255)
                                                                         aCCTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGttt                <  2:4643084/93‑1 (MQ=255)
                                                                          ccTTTCTGGGCGTTTTTCATTGTGGCGGCAATACGCGGATCTTTCGTCAACTCTTCCTCGTAAGACTTCAGCGCTACTTCACCCATCGGATTg               >  2:647779/1‑93 (MQ=255)
                                                                          ccTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTg               <  1:4943705/93‑1 (MQ=255)
                                                                           cTTTCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt              <  1:214073/93‑1 (MQ=255)
                                                                            tttCTGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc             >  2:2109071/1‑93 (MQ=255)
                                                                                tGGGCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTtta         >  2:6696614/1‑93 (MQ=255)
                                                                                 gggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttat        >  1:6996474/1‑93 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  1:1612219/93‑1 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  1:742620/1‑93 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       <  1:2981059/93‑1 (MQ=255)
                                                                                  ggCGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt       >  2:4372101/1‑93 (MQ=255)
                                                                                    cGTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTaa     <  1:2744492/93‑1 (MQ=255)
                                                                                     gTTTTTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc    >  2:3679043/1‑93 (MQ=255)
                                                                                      ttttTCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc   >  1:5422448/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCG  >  NC_000913/4242867‑4243046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑