BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	500,613	A→G	E422G (GAG→GGG)	gsk →	inosine‑guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	500,613	0	A	G	99.6%	842.6 / NA	270	E422G (GAG→GGG)	gsk	inosine‑guanosine kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (137/132); total (138/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

 aTCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCg                                                                                         <  2:7841829/93‑1 (MQ=255)

 aTCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCg                                                                                         >  2:7802652/1‑93 (MQ=255)

   cGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTg                                                                                       >  1:1046505/1‑93 (MQ=255)

   cGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTg                                                                                       >  1:5166718/1‑93 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                     >  1:6971481/1‑93 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                     >  2:529229/1‑93 (MQ=255)

       aGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGa                                                                                   <  1:4164713/93‑1 (MQ=255)

       aGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGa                                                                                   <  1:3450091/93‑1 (MQ=255)

        gcgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGAc                                                                                  <  2:3339004/93‑1 (MQ=255)

         cgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACa                                                                                 <  2:2564088/93‑1 (MQ=255)

          gcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAg                                                                                >  2:4868363/1‑93 (MQ=255)

          gcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAg                                                                                <  2:5095935/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                              <  2:2158866/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                              <  1:5432596/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                              <  1:1260358/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                              >  2:2715525/1‑93 (MQ=255)

              gtgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCGGGGGCGTGAAGACAGCC‑Tg                                                                           >  1:7692627/1‑93 (MQ=255)

               tgtgtAAAAATGAAAACCGTGCGAACTCTCAGGGACATAACCAGCATTCACCTCGTTTAAAGCGGGGCTTGCCGGGCTGTGAGCACAGCC‑ggg                                                                          >  2:5667557/1‑93 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGa                                                                         <  1:4611901/93‑1 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGa                                                                         <  1:6277382/93‑1 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGa                                                                         >  2:4137964/1‑93 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGa                                                                                   <  2:6459771/83‑1 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGaa                                                                        <  2:5197291/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAaga                                                                      <  1:5270239/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAaga                                                                      <  1:2553824/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAaga                                                                      <  1:6638904/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACGGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGAAAGCC‑GGGAagt                                                                      >  1:1924515/1‑92 (MQ=255)

                     aaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGt                                                                    <  2:6516137/93‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTc                                                                   <  1:2749410/93‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTc                                                                   <  1:3263287/93‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTc                                                                   <  2:7413948/93‑1 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCt                                                                  >  2:3098679/1‑93 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCt                                                                  <  1:5484869/93‑1 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCt                                                                  >  2:5917930/1‑93 (MQ=255)

                         tGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTa                                                                <  2:1717326/93‑1 (MQ=255)

                         tGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTa                                                                >  2:3437910/1‑93 (MQ=255)

                         tGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTa                                                                >  2:5555512/1‑93 (MQ=255)

                         cccTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTa                                                                >  2:6675981/3‑93 (MQ=255)

                          gCTAACCGTGTGCGCTATCAGGTACTGCACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAGGAGCATTAc                                                               >  2:2359390/1‑93 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTAc                                                               <  2:3475762/93‑1 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTAc                                                               <  1:5692483/93‑1 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGCTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTCGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTAc                                                               <  1:5543722/93‑1 (MQ=255)

                           cTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACt                                                              >  2:4467919/1‑93 (MQ=255)

                            tAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTg                                                             >  1:3579713/1‑93 (MQ=255)

                             aaCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTgg                                                            <  2:2833391/93‑1 (MQ=255)

                              aCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTggg                                                           <  2:5577638/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGa                                                          <  1:5974776/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGa                                                          <  1:6481062/93‑1 (MQ=255)

                               ccGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGa                                                          <  1:7530390/93‑1 (MQ=255)

                                cGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGAt                                                         <  2:1517549/93‑1 (MQ=255)

                                 gtgtGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTTGGAAGAGTCTTACTGGGAt                                                         <  2:6922413/93‑1 (MQ=255)

                                 gtgtGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATc                                                        >  1:4179147/1‑93 (MQ=255)

                                  tgtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGAC‑TGGAAGAGTCTTACTGGGATCg                                                       >  2:6614470/1‑93 (MQ=255)

                                   gtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGt                                                      <  1:6812064/93‑1 (MQ=255)

                                       gCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAg                                                  <  2:619978/93‑1 (MQ=255)

                                         tATCAGGTACTGAACCAGCATTCTCGTCGTTTAACGCGCGTCTTGCCGGGGCGTGAAGACAGCC‑TGTAAGAGTCTTACTGGGATCGTTAAGtt                                                <  2:5406235/93‑1 (MQ=255)

                                         tATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGtt                                                >  2:7707419/1‑93 (MQ=255)

                                         tATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGtt                                                >  2:7524853/1‑93 (MQ=255)

                                          aTCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTa                                               >  1:6740979/1‑93 (MQ=255)

                                          aTCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTa                                               >  1:7033020/1‑93 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              >  2:2070651/1‑93 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              >  2:466873/1‑93 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              >  2:5989127/1‑93 (MQ=255)

                                               gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtc                                          <  1:7275038/93‑1 (MQ=255)

                                                tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtcg                                         >  1:2204877/1‑93 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        <  2:478173/93‑1 (MQ=255)

                                                   tGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGtt                                      >  1:7309232/1‑93 (MQ=255)

                                                   cgAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGtt                                      <  1:783766/92‑1 (MQ=255)

                                                     aaCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCg                                    >  1:7163804/1‑93 (MQ=255)

                                                     aaCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCg                                    >  2:7257664/1‑93 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  <  1:1009941/93‑1 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  <  2:4267897/93‑1 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 >  1:3755793/1‑93 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 <  2:2887880/93‑1 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 <  1:3996939/93‑1 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 >  2:6839944/1‑93 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAg                                 <  1:1713193/93‑1 (MQ=255)

                                                        cAGCATTCACCTCGTTTAACGCGCGGCTCGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGCTATCGTCGGTTCGTAg                                 <  1:6416550/93‑1 (MQ=255)

                                                         aGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAgg                                <  1:5853642/93‑1 (MQ=255)

                                                         aGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAgg                                >  2:6417966/1‑93 (MQ=255)

                                                         aGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAgg                                >  2:3407870/1‑93 (MQ=255)

                                                          gCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGc                               <  2:40305/93‑1 (MQ=255)

                                                            aTTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGGTCGTTAAGTTATCGTCGGTTCGTAGGCCa                             >  2:3614955/1‑93 (MQ=255)

                                                            aTTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCa                             <  2:896789/93‑1 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            <  1:4734778/93‑1 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  2:3701432/1‑93 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           <  1:1942764/93‑1 (MQ=255)

                                                               cACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGAt                          >  1:4905890/1‑93 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:1374790/93‑1 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        <  2:2345211/93‑1 (MQ=255)

                                                                  cgcGTTTAACGCGCGACTTGCCGGGTCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAg                       <  2:5237792/91‑1 (MQ=255)

                                                                  cTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAg                       <  2:3864431/93‑1 (MQ=255)

                                                                    cGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGc                     <  2:1738470/93‑1 (MQ=255)

                                                                       ttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGtt                  <  1:5080142/93‑1 (MQ=255)

                                                                         aaCGCGCGGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCa                <  2:1241801/93‑1 (MQ=255)

                                                                              gcgGCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgccg           >  1:7875474/1‑93 (MQ=255)

                                                                                 gCTTGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCAt        >  2:4634395/1‑93 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      >  1:4431976/1‑93 (MQ=255)

                                                                                   ttGCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt      <  2:7610544/93‑1 (MQ=255)

                                                                                     gCCGGGGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTgg    >  2:1958005/1‑93 (MQ=255)

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCC‑TGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`