Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,212 A→G R413R (CGT→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2120AG99.7% 1162.7 / ‑6.3 376R413R (CGT→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (186/189);  total (186/190)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 389 total aligned reads displayed.

GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG  >  NC_000913/4270122‑4270301
                                                                                          |                                                                                         
  agaaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGcgcg                                                                                       >  1:7113946/1‑93 (MQ=255)
    aaTTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTc                                                                                     <  1:7228973/93‑1 (MQ=255)
      ttCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTccc                                                                                   >  1:35510/1‑93 (MQ=255)
       tCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCt                                                                                  <  1:2319178/93‑1 (MQ=255)
       tCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCt                                                                                  <  1:2492989/93‑1 (MQ=255)
        ccATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                 >  2:6428722/1‑93 (MQ=255)
        ccATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCtt                                                                                 >  1:2741893/1‑93 (MQ=255)
         cATCGCATGGCCAAGGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTc                                                                                <  2:6938479/93‑1 (MQ=255)
         cATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTc                                                                                >  1:3031247/1‑93 (MQ=255)
          aTCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCg                                                                               >  2:3144476/1‑93 (MQ=255)
           tCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGc                                                                              >  1:1154179/1‑93 (MQ=255)
            cGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCa                                                                             >  2:6525865/1‑93 (MQ=255)
             gCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAg                                                                            >  1:6251700/1‑93 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                           <  2:154456/93‑1 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                           <  1:638219/93‑1 (MQ=255)
              cATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGc                                                                           >  2:3031234/1‑93 (MQ=255)
               aTGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCt                                                                          <  2:2804327/93‑1 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGGCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         >  1:2087823/1‑93 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         <  1:2815640/93‑1 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         <  2:3315249/93‑1 (MQ=255)
                tGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTg                                                                         <  1:7139281/93‑1 (MQ=255)
                   ccAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcg                                                                      >  2:1274022/1‑93 (MQ=255)
                    cAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgc                                                                     >  1:85214/1‑93 (MQ=255)
                    cAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgc                                                                     >  2:5615785/1‑93 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                    >  1:4332787/1‑92 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                    >  2:4563983/1‑92 (MQ=255)
                     aaTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCGTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcg                                                                    >  2:610551/1‑92 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcgg                                                                   >  2:4143447/1‑91 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcgcgc                                                                   <  1:320046/93‑3 (MQ=255)
                      aTGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGgcccgc                                                                   >  1:2128467/1‑89 (MQ=255)
                       tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                  >  2:6663548/1‑93 (MQ=255)
                        gCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGTGCGCTTCCCGACGCCGGCGCGTCCCTTCGCAGCTGGCGCTCgg                                                                 >  1:7440344/1‑93 (MQ=255)
                            aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                             <  1:4371306/93‑1 (MQ=255)
                            aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                             <  1:4283298/93‑1 (MQ=255)
                            aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCACGCGCGTCCCTTCGCCTCTGGCGCGCGGACGa                                                             >  2:7340633/1‑93 (MQ=255)
                             tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                            >  2:4079876/1‑93 (MQ=255)
                                  gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTg                                                       >  1:176461/1‑93 (MQ=255)
                                   ggaTAGCGGGCGGCGGCGTATTCTCGCCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTTCTGa                                                      <  1:7003297/92‑1 (MQ=255)
                                   agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                      <  1:5192139/93‑1 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    >  1:6077606/1‑93 (MQ=255)
                                     aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                    <  2:2597178/93‑1 (MQ=255)
                                      tAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaa                                                   >  2:1148745/1‑93 (MQ=255)
                                      tAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaa                                                   >  2:1991171/1‑93 (MQ=255)
                                       aGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaaa                                                  <  1:4972975/93‑1 (MQ=255)
                                        gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                 <  1:4033603/93‑1 (MQ=255)
                                          aggcagCGGCGTATTCTCGACTGACGCGTGGCGGGCTTCCCGGCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCTGACGATTACTGATAAACtt                                               <  2:2149715/93‑1 (MQ=255)
                                          aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                               <  2:6702377/93‑1 (MQ=255)
                                          aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                               >  2:7814500/1‑93 (MQ=255)
                                          aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                               >  2:5227689/1‑93 (MQ=255)
                                           ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACTATTACTGATAAACTTg                                              <  1:7727400/93‑1 (MQ=255)
                                            gcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTgg                                             <  1:1389725/93‑1 (MQ=255)
                                               gcggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTa                                          <  1:5103449/93‑1 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                        <  1:3727126/93‑1 (MQ=255)
                                                 ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                        <  2:4011233/93‑1 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  2:3887725/1‑93 (MQ=255)
                                                  gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                       >  1:6658026/1‑93 (MQ=255)
                                                   cgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttc                                      <  2:3757575/93‑1 (MQ=255)
                                                    gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                     >  2:6226796/1‑93 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    <  2:7724929/93‑1 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    <  1:6855803/93‑1 (MQ=255)
                                                     tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                    >  1:575025/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGTGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   <  2:7766717/93‑1 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   >  1:5466533/1‑93 (MQ=255)
                                                      aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                   >  1:4693023/1‑93 (MQ=255)
                                                          tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                               >  2:2253202/1‑93 (MQ=255)
                                                           cGACATACGCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGCCTAATTCTTCGCGTa                              <  2:2932392/93‑1 (MQ=255)
                                                           cGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTa                              >  2:7887044/1‑93 (MQ=255)
                                                            gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                             >  2:7562542/1‑93 (MQ=255)
                                                             aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                            <  2:1379254/93‑1 (MQ=255)
                                                              cATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCCGACGATTACTGATAAACATTTATAATTCTTGGTGTaaca                           >  2:4456914/1‑90 (MQ=255)
                                                               aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                          >  2:5170008/1‑93 (MQ=255)
                                                               aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                          <  2:1411761/93‑1 (MQ=255)
                                                                tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                         <  1:1298582/93‑1 (MQ=255)
                                                                tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                         >  2:1769290/1‑93 (MQ=255)
                                                                 acacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCgcgc                        >  2:568281/1‑93 (MQ=255)
                                                                  cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                       <  2:4509005/93‑1 (MQ=255)
                                                                  cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCt                       <  1:156325/93‑1 (MQ=255)
                                                                   acGTGGCGCGCTTCCCGACGCAGGCGCGTTCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTg                      >  2:3488568/1‑93 (MQ=255)
                                                                     gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                    <  1:2471318/93‑1 (MQ=255)
                                                                     gTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGa                    <  2:5771434/93‑1 (MQ=255)
                                                                      tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                   <  1:3482754/93‑1 (MQ=255)
                                                                            gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc             <  2:7208450/93‑1 (MQ=255)
                                                                            gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc             <  1:113254/93‑1 (MQ=255)
                                                                            gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc             >  1:1049686/1‑93 (MQ=255)
                                                                            gcTCCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc             <  1:6573924/93‑1 (MQ=255)
                                                                             cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct            <  1:7824820/93‑1 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc           >  2:2055546/1‑93 (MQ=255)
                                                                              ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAACGCGCTGGATTCCGtctc           >  2:486604/1‑93 (MQ=255)
                                                                               tCCCGACGCTGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct          <  2:4219748/93‑1 (MQ=255)
                                                                                cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt         <  1:2593801/93‑1 (MQ=255)
                                                                                 ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt        <  1:5569421/93‑1 (MQ=255)
                                                                                 ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt        <  1:5336931/93‑1 (MQ=255)
                                                                                 ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt        >  1:381832/1‑93 (MQ=255)
                                                                                  cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta       >  2:2496083/1‑93 (MQ=255)
                                                                                  cGACGCAGGCGCGTCCCGTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta       <  1:4124096/93‑1 (MQ=255)
                                                                                   gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat      <  2:552343/93‑1 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata     <  1:6816219/93‑1 (MQ=255)
                                                                                    aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata     <  1:6509935/93‑1 (MQ=255)
                                                                                     cGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAg    <  1:4633464/93‑1 (MQ=255)
                                                                                      gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgc   <  1:225945/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAAGAATTCCATCGCATGACCAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG  >  NC_000913/4270122‑4270301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑