BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,491	A→G	V101A (GTA→GCA)	yjcH ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,491	0	A	G	99.5%	1253.6 / ‑5.7	397	V101A (GTA→GCA)	yjcH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (194/200); total (195/202)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 414 total aligned reads displayed.

CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC  >  NC_000913/4282401‑4282580

 tctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCt                                                                                        >  2:7828748/1‑93 (MQ=255)

   tACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCa                                                                                      <  2:3678869/93‑1 (MQ=255)

    aCGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCAt                                                                                     <  1:7079757/93‑1 (MQ=255)

    aCGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCAt                                                                                     <  2:3824025/93‑1 (MQ=255)

    aCGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCAt                                                                                     >  2:4161012/1‑93 (MQ=255)

     cGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATg                                                                                    <  1:3901251/93‑1 (MQ=255)

      ggCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTATTCATGATGCTTGTGCCTCATGc                                                                                   >  2:5634861/1‑93 (MQ=255)

       gCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCa                                                                                  >  1:3363511/1‑93 (MQ=255)

          ccGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGa                                                                               >  2:2181706/1‑93 (MQ=255)

           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              <  1:2858084/93‑1 (MQ=255)

             cTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACtt                                                                            >  1:3744472/1‑93 (MQ=255)

              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  2:3387252/1‑93 (MQ=255)

               aaTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCa                                                                          <  1:6755320/93‑1 (MQ=255)

                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         >  1:7344824/1‑93 (MQ=255)

                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         <  1:2489288/93‑1 (MQ=255)

                 tAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCatt                                                                        <  1:2768418/93‑1 (MQ=255)

                  aGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCatta                                                                       <  2:1008279/93‑1 (MQ=255)

                   gCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCattat                                                                      >  2:4050008/1‑93 (MQ=255)

                    cATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCattatt                                                                     <  1:210461/93‑1 (MQ=255)

                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:878791/93‑1 (MQ=255)

                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:6163197/93‑1 (MQ=255)

                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   >  2:6502039/1‑93 (MQ=255)

                         gcggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGAc                                                                <  2:3062337/93‑1 (MQ=255)

                         gcggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGAc                                                                >  1:1418347/1‑93 (MQ=255)

                         gcggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTCTGCCTCATGCAGGACTTCATTATTAAGAc                                                                >  2:5249984/1‑93 (MQ=255)

                          cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACg                                                               >  1:4463847/1‑93 (MQ=255)

                          cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACg                                                               <  1:1485283/93‑1 (MQ=255)

                          cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACg                                                               <  1:5392314/93‑1 (MQ=255)

                          cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTCGTGCCTCATGCAGGACTTCATTATTAAGACg                                                               <  1:1691988/93‑1 (MQ=255)

                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              <  1:5183823/93‑1 (MQ=255)

                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              >  1:6376055/1‑93 (MQ=255)

                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             >  2:4345613/1‑93 (MQ=255)

                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             >  2:50166/1‑93 (MQ=255)

                              gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCg                                                           <  2:1939433/93‑1 (MQ=255)

                              gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCg                                                           >  2:6743070/1‑93 (MQ=255)

                              gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCg                                                           <  1:1173486/93‑1 (MQ=255)

                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          >  2:6298553/1‑93 (MQ=255)

                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGCCTTCATTATTAAGACGGTCGAAtt                                                       >  2:2790470/1‑93 (MQ=255)

                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                       >  1:6893620/1‑93 (MQ=255)

                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                       <  2:3199935/93‑1 (MQ=255)

                                   cggCGAAAGTGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                                                      <  1:36617/91‑1 (MQ=255)

                                   cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                                                      <  2:475855/93‑1 (MQ=255)

                                   cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                                                      <  2:1500370/93‑1 (MQ=255)

                                    tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                                                     <  1:1935413/93‑1 (MQ=255)

                                    tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                                                     >  2:7637325/1‑93 (MQ=255)

                                      cGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGcc                                                   >  2:1748596/1‑93 (MQ=255)

                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 <  1:6835029/93‑1 (MQ=255)

                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 >  1:2291195/1‑93 (MQ=255)

                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 >  1:6533500/1‑93 (MQ=255)

                                           gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcg                                              >  1:7527208/1‑93 (MQ=255)

                                            ggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc                                             >  2:1346976/1‑93 (MQ=255)

                                            ggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgc                                             >  2:6444240/1‑93 (MQ=255)

                                                 gtgGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcgcccgcc                                        <  2:6370864/93‑1 (MQ=255)

                                                    gcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa                                     <  2:4218006/93‑1 (MQ=255)

                                                      ggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATg                                   >  1:7914798/1‑93 (MQ=255)

                                                        cAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTa                                 <  1:4867481/93‑1 (MQ=255)

                                                         aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAg                                <  2:706034/93‑1 (MQ=255)

                                                          aGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGa                               <  2:6857831/93‑1 (MQ=255)

                                                          aGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGa                               <  1:7228702/93‑1 (MQ=255)

                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              >  1:267408/1‑93 (MQ=255)

                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              <  1:6281805/93‑1 (MQ=255)

                                                             gcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAc                            >  2:5514495/1‑93 (MQ=255)

                                                             gcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAc                            >  2:5420052/1‑93 (MQ=255)

                                                              ccGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAcc                           >  1:4939097/1‑93 (MQ=255)

                                                                 tCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCggg                        >  2:1098023/1‑92 (MQ=255)

                                                                 tCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGt                        >  1:7122666/1‑93 (MQ=255)

                                                                  cAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTg                       <  2:3889120/93‑1 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  2:6097562/93‑1 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      >  1:2225193/1‑93 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  2:5846075/93‑1 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  1:5769254/93‑1 (MQ=255)

                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  1:3133099/93‑1 (MQ=255)

                                                                    gAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgag                     >  2:6679150/1‑93 (MQ=255)

                                                                    gAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgag                     >  2:7439047/1‑93 (MQ=255)

                                                                     aaCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgaga                    >  1:1037469/1‑93 (MQ=255)

                                                                     aaCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgaga                    >  1:5946626/1‑93 (MQ=255)

                                                                     aaCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgaga                    >  1:767974/1‑93 (MQ=255)

                                                                      aCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGaa                   <  2:1612689/93‑1 (MQ=255)

                                                                      aCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGaa                   <  2:6643272/93‑1 (MQ=255)

                                                                      aCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGaa                   >  2:3008151/1‑93 (MQ=255)

                                                                       ctctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAc                  <  1:7800284/93‑1 (MQ=255)

                                                                       ctctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAc                  <  2:3480001/93‑1 (MQ=255)

                                                                        tctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACa                 >  1:4322171/1‑93 (MQ=255)

                                                                         ctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaa                >  1:3192849/1‑93 (MQ=255)

                                                                         ctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaa                <  1:2420924/93‑1 (MQ=255)

                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               >  1:4597744/1‑93 (MQ=255)

                                                                           tttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg              <  2:7735677/93‑1 (MQ=255)

                                                                           tttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg              <  2:4595063/93‑1 (MQ=255)

                                                                           tttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg              <  2:2654109/93‑1 (MQ=255)

                                                                             tCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGga            <  2:1687155/93‑1 (MQ=255)

                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  1:959040/1‑93 (MQ=255)

                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  1:3522378/1‑93 (MQ=255)

                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          <  1:2939246/93‑1 (MQ=255)

                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         >  1:6121115/1‑93 (MQ=255)

                                                                                  atgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATca       <  2:4525517/93‑1 (MQ=255)

                                                                                  atgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATca       <  2:5495379/93‑1 (MQ=255)

                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  2:2476759/1‑93 (MQ=255)

                                                                                   tgCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      <  2:7221065/93‑1 (MQ=255)

                                                                                    gCTTGTGCCTAGTGCGGGCCTTCATTGTTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcaca     <  1:564770/93‑1 (MQ=255)

                                                                                     cTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACaa    >  2:5156007/1‑93 (MQ=255)

CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC  >  NC_000913/4282401‑4282580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`