BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,295	A→G	V219A (GTA→GCA)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,295	0	A	G	99.7%	1037.9 / ‑5.5	329	V219A (GTA→GCA)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (167/161); total (167/162)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 350 total aligned reads displayed.

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

tGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCATCAGTgcc                                                                                         >  1:1883466/1‑93 (MQ=255)

   tGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgccacc                                                                                      >  2:5915534/1‑93 (MQ=255)

   tGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgccacc                                                                                      >  1:3819154/1‑93 (MQ=255)

   tGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTgccacc                                                                                      >  1:2244638/1‑93 (MQ=255)

     cTGATCTCCCCCTTGCTCAACCAGGTCGGGCCACCACAGGTCCCCCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCcaccac                                                                                    <  2:2752304/93‑1 (MQ=255)

     cTGACCGCTCGCTTCCTCACCCAGGTCGTCCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCcaccac                                                                                    <  1:3184218/93‑1 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   <  1:3472050/93‑1 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  1:7398523/1‑93 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   >  2:1203504/1‑93 (MQ=255)

      tGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTCCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACcaca                                                                                   <  2:3421783/93‑1 (MQ=255)

       gATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACAt                                                                                  >  1:6095547/1‑93 (MQ=255)

         tcgctcgcTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGc                                                                                >  1:6325362/1‑93 (MQ=255)

           gctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctc                                                                              >  2:5965685/1‑93 (MQ=255)

           gctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctc                                                                              >  1:5758536/1‑93 (MQ=255)

            ctcgctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGctct                                                                             >  2:314533/1‑93 (MQ=255)

             ccgctTCCTCACCCAGGTCGTCCCACCACAGGTCGCCCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTa                                                                            <  1:6655662/92‑1 (MQ=255)

                ctTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGc                                                                         >  2:753614/1‑93 (MQ=255)

                 ttGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCt                                                                        <  1:7836022/93‑1 (MQ=255)

                 ttGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCt                                                                        >  2:5586159/1‑93 (MQ=255)

                   tctaaaCCAGGTCGCCCCCCCACTGGACGCGCCTTTCCTGCAAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTgg                                                                      <  1:4238460/89‑1 (MQ=255)

                   gcccAACCAGCCCGTGCCCCCACGGGCCGCGCCCTTCCTCCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTgg                                                                      <  1:6537301/90‑1 (MQ=255)

                    cTCACCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                     <  1:453503/93‑1 (MQ=255)

                    cTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTggg                                                                     >  1:493915/1‑92 (MQ=255)

                    cTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGt                                                                     <  2:4753833/93‑1 (MQ=255)

                     tCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTg                                                                    >  1:46777/1‑93 (MQ=255)

                     ccAACCCGGTCGTGCCACCACAGGTCGCCCCTTTCCGGCTAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTg                                                                    <  1:830103/92‑1 (MQ=255)

                        aCCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACg                                                                 >  2:2392629/1‑93 (MQ=255)

                         ccAGGTCGTGCCACCCCAGGCCCCGCCTTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGt                                                                <  1:480550/93‑1 (MQ=255)

                          cAGGTGGTGCCCCCACAGGTCGCGCCCTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               <  2:3509307/93‑1 (MQ=255)

                          cAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGtt                                                               >  2:6170454/1‑93 (MQ=255)

                            ggTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCg                                                             >  2:4988476/1‑93 (MQ=255)

                              tCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCggg                                                           >  1:1650928/1‑93 (MQ=255)

                               cGTGCCACCACAGGTCGCGCCCTTCCTGCCATTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGt                                                          <  1:2145600/93‑1 (MQ=255)

                               cGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGt                                                          >  1:3417976/1‑93 (MQ=255)

                                gTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCTCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGCCGTTCgggct                                                         >  1:3121592/1‑91 (MQ=255)

                                gTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         >  1:4091739/1‑93 (MQ=255)

                                gTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGtt                                                         >  1:1266003/1‑93 (MQ=255)

                                 tGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGttt                                                        >  1:3654704/1‑93 (MQ=255)

                                   ccaccaCAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGttttt                                                      >  1:5528363/1‑93 (MQ=255)

                                     cccagAGGTCGCGCCCTTCCTGCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCa                                                    <  2:13972/92‑1 (MQ=255)

                                     cccacAGGTCCCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCa                                                    <  1:2624367/92‑1 (MQ=255)

                                      ccacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAg                                                   >  2:6426213/1‑93 (MQ=255)

                                      ccacAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAg                                                   >  1:3999326/1‑93 (MQ=255)

                                           ggTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGGTTTTTAGCGCGt                                              >  2:6019175/1‑93 (MQ=255)

                                             tCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtca                                            >  2:4941946/1‑93 (MQ=255)

                                             tCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtca                                            >  1:1937467/1‑93 (MQ=255)

                                              cggccccTCCCGGCCAGTCATTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcat                                           <  2:2196170/89‑1 (MQ=255)

                                               gcgcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatc                                          >  1:5293638/1‑93 (MQ=255)

                                               gcgcCCTTCCTGCCACTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatc                                          <  1:5547750/93‑1 (MQ=255)

                                                cgcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGtcatca                                         >  2:4585922/1‑93 (MQ=255)

                                                 gcCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        >  2:5688718/1‑93 (MQ=255)

                                                 cccctccctGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCaa                                        <  2:6202920/92‑1 (MQ=255)

                                                  cccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       >  1:2848490/1‑93 (MQ=255)

                                                  cccTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       >  2:2501802/1‑93 (MQ=255)

                                                  cccTTCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAAc                                       <  1:1442445/93‑1 (MQ=255)

                                                    cTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGt                                     <  2:6280897/93‑1 (MQ=255)

                                                     ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    >  2:7355318/1‑93 (MQ=255)

                                                     ttCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    >  2:2594609/1‑93 (MQ=255)

                                                     ttCCTGCCAGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    <  1:1520705/93‑1 (MQ=255)

                                                     tcccTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGtt                                    <  2:5329861/91‑1 (MQ=255)

                                                        cTGCCAGTCATTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGttttt                                 <  2:3785292/93‑1 (MQ=255)

                                                           caaGTAAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttctt                              <  2:1051160/91‑1 (MQ=255)

                                                            cattCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  2:2228141/90‑1 (MQ=255)

                                                            cAGTCAATGTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttc                             <  2:1613174/93‑1 (MQ=255)

                                                             cgTCAATTTTCCCGCCAGTCCGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTttcttcc                            <  1:7031281/92‑2 (MQ=255)

                                                             aGTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            >  2:3875981/1‑93 (MQ=255)

                                                             aGTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCa                            <  2:1778779/93‑1 (MQ=255)

                                                              gTCAATTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           >  2:1891993/1‑93 (MQ=255)

                                                              gTCAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAg                           <  1:7244623/93‑1 (MQ=255)

                                                                cAATTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCg                         <  1:5415322/93‑1 (MQ=255)

                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        <  1:4456301/93‑1 (MQ=255)

                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        >  2:5477385/1‑93 (MQ=255)

                                                                 aaTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCgg                        <  2:3598030/93‑1 (MQ=255)

                                                                  aTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       <  2:7508498/93‑1 (MQ=255)

                                                                  aTTTTCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       >  1:3039958/1‑93 (MQ=255)

                                                                  aTTTCCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGa                       <  1:2338046/93‑1 (MQ=255)

                                                                   ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGaa                      >  1:283053/1‑93 (MQ=255)

                                                                   ttttCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGaa                      <  1:6799412/93‑1 (MQ=255)

                                                                     tccccGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATa                    <  2:7022077/91‑1 (MQ=255)

                                                                      tCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATAc                   >  2:4361790/1‑93 (MQ=255)

                                                                      tCCCGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATAc                   <  2:6724273/93‑1 (MQ=255)

                                                                         ctccAGTACGCTTCAGTGCCACAACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgc                <  2:5880803/91‑1 (MQ=255)

                                                                         cGCCAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgc                <  2:4509726/93‑1 (MQ=255)

                                                                           ccAGTACGCTTCAGTGCCCCCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTgcgc              <  1:5223569/93‑1 (MQ=255)

                                                                            cAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc             >  1:5209883/1‑93 (MQ=255)

                                                                            cAGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGcc             <  2:326955/93‑1 (MQ=255)

                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            >  2:2825093/1‑93 (MQ=255)

                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            >  2:785055/1‑93 (MQ=255)

                                                                             aGTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGccc            <  1:508223/93‑1 (MQ=255)

                                                                              gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg           >  2:6623530/1‑93 (MQ=255)

                                                                              gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCg           >  1:4616070/1‑93 (MQ=255)

                                                                              gTACGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCCCCCg           >  1:5884016/1‑93 (MQ=255)

                                                                                aCGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgc         >  1:4340916/1‑93 (MQ=255)

                                                                                 cGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcc        >  1:4859386/1‑92 (MQ=255)

                                                                                 cGCTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgca        >  2:1266992/1‑93 (MQ=255)

                                                                                   cTTCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacg      >  2:2858457/1‑93 (MQ=255)

                                                                                    ttCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgc     >  2:3949891/1‑93 (MQ=255)

                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    <  2:7916310/93‑1 (MQ=255)

                                                                                     tCAGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGgcacgca    >  2:101135/1‑93 (MQ=255)

                                                                                       aGTGCCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGcaca  <  1:3287457/93‑1 (MQ=255)

TGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCGCCAGTACGCTTCAGTACCACCACATGCTCTACGCTGGTGACGTTCGGGTTTTTCAGCGCGTCATCAACGTTTTTCTTCAGCGGAATACTGCGCCCGGCACGCACA  >  NC_000913/4284205‑4284384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`