BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,298,670	A→G	L616S (TTG→TCG)	yjcQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,298,670	0	A	G	99.7%	1029.4 / ‑5.3	331	L616S (TTG→TCG)	yjcQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (127/203); total (127/204)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 351 total aligned reads displayed.

CAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATT  >  NC_000913/4298580‑4298759

cagcagACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAg                                                                                         <  1:349765/93‑1 (MQ=255)

 agcagACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGc                                                                                        <  2:6746868/93‑1 (MQ=255)

  gcagACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCg                                                                                       <  1:3916343/93‑1 (MQ=255)

  gcagACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCg                                                                                       <  2:5738145/93‑1 (MQ=255)

   cagACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCgg                                                                                      >  2:6861265/1‑93 (MQ=255)

     gACGTGTTAGTTGACGATTGATCATTGCGTGGCTCCTTGTTTCTGTTCCGTGGCGGGCGGTAAATTCGGGGTTGTCCAGCCTGGCTAGCgggg                                                                                    >  2:7242911/1‑92 (MQ=255)

       cGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGa                                                                                  <  2:7431765/93‑1 (MQ=255)

        gTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGaa                                                                                 >  2:276095/1‑93 (MQ=255)

        gTGAAAGTTGACGATTGATCATTGAGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAATTTGTCGAGCGGGCgga                                                                                 >  2:2341085/1‑91 (MQ=255)

         tGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGaaa                                                                                >  1:7201629/1‑93 (MQ=255)

         tGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGaaa                                                                                >  2:6143117/1‑93 (MQ=255)

            aaGTTGACTATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGc                                                                             <  1:1597443/93‑1 (MQ=255)

            aaGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGc                                                                             <  2:6279315/93‑1 (MQ=255)

            aaGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGc                                                                             >  2:1690379/1‑93 (MQ=255)

            aaGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGc                                                                             <  1:903500/93‑1 (MQ=255)

             aGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCt                                                                            <  1:4238962/93‑1 (MQ=255)

             aGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCt                                                                            <  2:2158313/93‑1 (MQ=255)

              gtcgACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTg                                                                           <  1:2371896/90‑1 (MQ=255)

              gTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTg                                                                           <  1:494836/93‑1 (MQ=255)

              gTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCAAAAAGCTg                                                                           <  2:265397/93‑1 (MQ=255)

               ttGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTgg                                                                          >  1:6263740/1‑93 (MQ=255)

                tGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCTGGCGAAAAGCTGGc                                                                         >  2:5852947/1‑93 (MQ=255)

                  aCGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAg                                                                       <  2:5557184/93‑1 (MQ=255)

                   cGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGa                                                                      <  1:713283/93‑1 (MQ=255)

                    gATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGag                                                                     >  1:7931674/1‑92 (MQ=255)

                    gATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGAc                                                                     >  2:387245/1‑93 (MQ=255)

                    gATGGATCAGTCGGTGGCTCCTTGTGCCTGTTCGGTGGCGGTCTTTAAGGCCGGGGCGGTCCAGTCGGGCGAGCGGGCGAAAAGCTGGCAGAc                                                                     <  2:3000951/93‑1 (MQ=255)

                     aTTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACg                                                                    >  1:1379039/1‑93 (MQ=255)

                      ttGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACgt                                                                   <  1:5569275/93‑1 (MQ=255)

                        taTCATTGCGCGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACgtgt                                                                 <  1:2257325/92‑1 (MQ=255)

                        gATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACgtgt                                                                 <  2:1747926/93‑1 (MQ=255)

                        gATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACgtgt                                                                 >  2:4714838/1‑93 (MQ=255)

                        gATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACgtgt                                                                 >  1:1138962/1‑93 (MQ=255)

                         aTCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGtt                                                                <  2:4024488/93‑1 (MQ=255)

                           cATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGc                                                              <  1:2993175/93‑1 (MQ=255)

                            aTTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCt                                                             <  2:1846468/93‑1 (MQ=255)

                              tGCGTGGCTCCTTGTGCCTGTTCCGTAGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCAcc                                                           <  1:6004075/93‑1 (MQ=255)

                               gCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCt                                                          >  2:110152/1‑93 (MQ=255)

                                cGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTg                                                         <  2:3365560/93‑1 (MQ=255)

                                 gTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGt                                                        >  1:374722/1‑93 (MQ=255)

                                  tGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGtt                                                       >  2:4258786/1‑93 (MQ=255)

                                  tGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGtt                                                       <  2:429314/93‑1 (MQ=255)

                                   ggCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGttt                                                      <  1:2089819/93‑1 (MQ=255)

                                      tCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaa                                                   >  1:4424371/1‑93 (MQ=255)

                                       ccTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTTCTGTTTttat                                                  >  1:6754854/1‑93 (MQ=255)

                                       ccTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtcat                                                  >  2:3404403/1‑93 (MQ=255)

                                       ccTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaat                                                  >  2:367899/1‑93 (MQ=255)

                                        cTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaata                                                 <  2:7681754/93‑1 (MQ=255)

                                         ttGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaatta                                                >  2:7059370/1‑91 (MQ=255)

                                         ttGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaataa                                                >  1:7488427/1‑93 (MQ=255)

                                         ttGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaataa                                                >  1:1957568/1‑93 (MQ=255)

                                         ttGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaataa                                                <  1:5735993/93‑1 (MQ=255)

                                          tgtgCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAg                                               <  1:4586310/93‑1 (MQ=255)

                                           gtgCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAgg                                              <  2:7727689/93‑1 (MQ=255)

                                            tgCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGGAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGt                                             >  1:3530900/1‑93 (MQ=255)

                                            tgCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGt                                             <  1:4801963/93‑1 (MQ=255)

                                            gtccTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGt                                             <  2:6534455/91‑1 (MQ=255)

                                             gCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAGGGTg                                            >  1:640261/1‑93 (MQ=255)

                                             gCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTg                                            <  2:5143849/93‑1 (MQ=255)

                                             gCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTg                                            <  2:7387379/93‑1 (MQ=255)

                                             gCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTg                                            <  2:6947448/93‑1 (MQ=255)

                                              ccggTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTgg                                           <  1:1687503/90‑1 (MQ=255)

                                              ccTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTgg                                           >  2:6566336/1‑93 (MQ=255)

                                               cTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTggg                                          >  2:7316157/1‑93 (MQ=255)

                                               cTGTTCCGTGGCGGGCGTTAATGCCAGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTggg                                          <  1:5154723/93‑1 (MQ=255)

                                                tGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGc                                         >  1:2505141/1‑93 (MQ=255)

                                                 gTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCa                                        <  1:3478886/93‑1 (MQ=255)

                                                    ccGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGa                                     <  1:612704/93‑1 (MQ=255)

                                                      gTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATg                                   <  1:6929875/93‑1 (MQ=255)

                                                      gTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATg                                   <  2:5769246/93‑1 (MQ=255)

                                                       tGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATgg                                  <  1:3137310/93‑1 (MQ=255)

                                                          cgggcgTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCt                               <  2:5065453/93‑1 (MQ=255)

                                                            ggcgTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTga                             >  1:1704860/1‑93 (MQ=255)

                                                              cgTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTgaga                           <  2:6510211/93‑1 (MQ=255)

                                                               gTTAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGagag                          <  2:6366120/93‑1 (MQ=255)

                                                                ttAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAgg                         <  1:292798/93‑1 (MQ=255)

                                                                 tAATGCCTGGGCTGGCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGc                        <  1:5390879/93‑1 (MQ=255)

                                                                 tAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGc                        <  1:954091/93‑1 (MQ=255)

                                                                 tAATGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGc                        <  2:2573838/93‑1 (MQ=255)

                                                                  aaTGCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCg                       <  1:4885289/93‑1 (MQ=255)

                                                                     gCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgt                    <  2:2940857/93‑1 (MQ=255)

                                                                     gCCGGGGCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgt                    <  2:1525158/93‑1 (MQ=255)

                                                                        ggggCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtgtt                 >  1:6665009/1‑92 (MQ=255)

                                                                        ggggCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGtc                 <  1:7261605/93‑1 (MQ=255)

                                                                        ggggCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGtc                 <  2:6068083/93‑1 (MQ=255)

                                                                          ggCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGtctc               <  1:7400780/93‑1 (MQ=255)

                                                                           gCTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTctct              >  1:5799763/1‑93 (MQ=255)

                                                                            cTGTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCtt             >  2:1949712/1‑93 (MQ=255)

                                                                              gTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGGGGGGGTCGCttta           >  1:1013615/1‑91 (MQ=255)

                                                                              gTCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCa           <  1:2177989/93‑1 (MQ=255)

                                                                               tCCAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCtttca          >  1:4156435/1‑90 (MQ=255)

                                                                                ccAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAg         <  1:7642619/93‑1 (MQ=255)

                                                                                 cAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGc        <  2:5434320/93‑1 (MQ=255)

                                                                                 cAGTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCCAGc        >  1:2875821/1‑93 (MQ=255)

                                                                                   gTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCGAGCGt      >  2:2384143/1‑93 (MQ=255)

                                                                                   gTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGt      <  2:4869815/93‑1 (MQ=255)

                                                                                   gTCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGGTTCTTTAAGCGt      >  2:45580/1‑93 (MQ=255)

                                                                                    tCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGtt     <  2:7813451/93‑1 (MQ=255)

                                                                                    tCTGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGtt     >  2:2096735/1‑93 (MQ=255)

                                                                                      tGGCGAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTAt   >  1:522388/1‑93 (MQ=255)

CAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATT  >  NC_000913/4298580‑4298759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`