BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,369,637	T→C	R345R (CGT→CGC)	mopA →	GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,369,637	0	T	C	99.5%	1207.3 / ‑5.8	385	R345R (CGT→CGC)	mopA	GroEL
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (185/198); total (187/198)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 407 total aligned reads displayed.

AGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCAGGCGG  >  NC_000913/4369547‑4369726

aGACCTGGGTCAGTCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgcgt                                                                                         <  2:1803335/93‑1 (MQ=255)

aGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgcgt                                                                                         <  2:5021490/93‑1 (MQ=255)

aGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCgcgt                                                                                         <  1:3525756/93‑1 (MQ=255)

  aCCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTg                                                                                       >  1:5584341/1‑93 (MQ=255)

   ccTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGCGAAGAAGCTGCAATCCAGGGCCGCGTTGc                                                                                      >  1:4146621/1‑93 (MQ=255)

    cTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCt                                                                                     <  1:2004650/93‑1 (MQ=255)

    cTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCt                                                                                     >  1:7080834/1‑93 (MQ=255)

      gggTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCa                                                                                   <  1:3836317/93‑1 (MQ=255)

        gTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGa                                                                                 >  2:681347/1‑93 (MQ=255)

          cAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATc                                                                               <  2:1339856/93‑1 (MQ=255)

          cAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATc                                                                               <  1:6320362/93‑1 (MQ=255)

           aGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATcc                                                                              >  1:3024347/1‑93 (MQ=255)

            ggCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCg                                                                             <  1:7318114/93‑1 (MQ=255)

            ggCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGCGGGTCAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCg                                                                             >  1:1568126/1‑93 (MQ=255)

               tAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTca                                                                          <  2:38361/93‑1 (MQ=255)

                aaaCGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTcag                                                                         >  1:4988052/1‑93 (MQ=255)

                aaaCGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTcag                                                                         <  1:374443/93‑1 (MQ=255)

                 aaCGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTcagc                                                                        <  1:7554630/93‑1 (MQ=255)

                    gtgtTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGa                                                                     >  1:131417/1‑93 (MQ=255)

                    gtgtTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGa                                                                     <  1:430882/93‑1 (MQ=255)

                     tgttgtGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGAt                                                                    <  1:964236/93‑1 (MQ=255)

                     tgttgtGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGAt                                                                    >  2:87778/1‑93 (MQ=255)

                      gttgtGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGAtt                                                                   >  2:6629913/1‑93 (MQ=255)

                      gttgtGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGAtt                                                                   >  2:6182653/1‑93 (MQ=255)

                      gttgtGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGAtt                                                                   >  1:3131590/1‑93 (MQ=255)

                       ttgtGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTg                                                                  <  2:1626553/93‑1 (MQ=255)

                        tgtgATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTga                                                                 <  2:5538853/93‑1 (MQ=255)

                         gtgATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTgaa                                                                <  2:5127696/93‑1 (MQ=255)

                          tgATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTgaag                                                               >  1:3697284/1‑93 (MQ=255)

                          tgATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTgaag                                                               >  2:4040603/1‑93 (MQ=255)

                                 caaAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAAc                                                        <  2:3698835/93‑1 (MQ=255)

                                 caaAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAAc                                                        >  2:2970955/1‑93 (MQ=255)

                                  aaaGAAACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACt                                                       >  2:5265714/1‑93 (MQ=255)

                                       caccaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGa                                                  >  1:6683309/1‑93 (MQ=255)

                                        accaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGAc                                                 >  2:692062/1‑93 (MQ=255)

                                        accaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGAc                                                 <  2:199435/93‑1 (MQ=255)

                                        accaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGAc                                                 <  2:1594883/93‑1 (MQ=255)

                                        accaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGAc                                                 <  2:1203752/93‑1 (MQ=255)

                                         ccaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGCAGCAACTTCTGACt                                                >  2:3542657/1‑93 (MQ=255)

                                         ccaccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACt                                                >  2:2257495/1‑93 (MQ=255)

                                          caccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTa                                               >  1:72373/1‑93 (MQ=255)

                                           cccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTAc                                              <  1:1027988/92‑1 (MQ=255)

                                           accacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTAc                                              >  1:5812261/1‑93 (MQ=255)

                                            ccacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACg                                             >  1:7467285/1‑93 (MQ=255)

                                             cacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGa                                            <  2:391445/93‑1 (MQ=255)

                                             cacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGa                                            <  1:1856358/93‑1 (MQ=255)

                                             cacTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGa                                            >  1:5173208/1‑93 (MQ=255)

                                              acTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGAc                                           <  2:786448/93‑1 (MQ=255)

                                              acTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGGTGCTCAGATCCGTAAGCAGATTGAAGAAGCCACATCTGACTTCGAc                                           >  2:2836787/1‑93 (MQ=255)

                                                tATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCg                                         >  1:4060467/1‑93 (MQ=255)

                                                tATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCg                                         <  1:2784658/93‑1 (MQ=255)

                                                tATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCg                                         >  2:743960/1‑93 (MQ=255)

                                                 atcatcGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGt                                        >  1:6421600/1‑93 (MQ=255)

                                                  tcatcGATGGCGTGTGCGACGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTg                                       >  2:6472215/1‑93 (MQ=255)

                                                     tcGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaa                                    >  2:2444940/1‑93 (MQ=255)

                                                     acGATGGCGTGGGTGAAAAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaa                                    <  1:5289107/92‑1 (MQ=255)

                                                       gATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaaaa                                  <  1:2343709/93‑1 (MQ=255)

                                                       gATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaaaa                                  >  2:1263857/1‑93 (MQ=255)

                                                       gATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaaaa                                  >  2:5602466/1‑93 (MQ=255)

                                                       gATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaaaa                                  <  2:1937751/93‑1 (MQ=255)

                                                       gATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGaaaaa                                  <  2:6519084/93‑1 (MQ=255)

                                                        aTGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAAc                                 <  2:7936698/93‑1 (MQ=255)

                                                         tGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACt                                <  1:2165816/93‑1 (MQ=255)

                                                         tGGCGGGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACt                                <  2:5756053/93‑1 (MQ=255)

                                                            cGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCa                             <  2:7315695/93‑1 (MQ=255)

                                                            cGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCa                             <  1:4451776/93‑1 (MQ=255)

                                                            cGTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCa                             <  2:1423847/93‑1 (MQ=255)

                                                             gTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAg                            >  2:5413084/1‑93 (MQ=255)

                                                             gTGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAg                            <  1:6124295/93‑1 (MQ=255)

                                                              tGGGTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAgg                           >  2:258323/1‑93 (MQ=255)

                                                               ngnTGAANAAGCTGCAATCCANGNCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGa                          <  1:6524123/92‑1 (MQ=255)

                                                               gggTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGa                          <  1:2628718/93‑1 (MQ=255)

                                                               gggTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGa                          >  2:4751163/1‑93 (MQ=255)

                                                                ggTGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGaa                         >  2:4519018/1‑93 (MQ=255)

                                                                  tGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAAcg                       <  1:618568/93‑1 (MQ=255)

                                                                  tGAAGAAGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAAcg                       >  1:1498344/1‑93 (MQ=255)

                                                                   gaagaaGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAAcgc                      <  1:1122196/93‑1 (MQ=255)

                                                                   gaagaaGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAAcgc                      >  2:6383169/1‑93 (MQ=255)

                                                                   gaagaaGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAAcgc                      <  2:4251299/93‑1 (MQ=255)

                                                                   gaagaaGCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAAcgc                      >  2:2041764/1‑93 (MQ=255)

                                                                      gaagCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                   <  2:2701946/93‑1 (MQ=255)

                                                                      gaagCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTa                   <  1:4873493/93‑1 (MQ=255)

                                                                        agCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGc                 >  2:4626804/1‑93 (MQ=255)

                                                                         gCTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCg                <  1:3379902/93‑1 (MQ=255)

                                                                          cTGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGa               <  2:7602151/93‑1 (MQ=255)

                                                                           tGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGaa              <  2:4347759/93‑1 (MQ=255)

                                                                           tGCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGaa              >  1:7772657/1‑93 (MQ=255)

                                                                            gCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGaaa             >  1:4813603/1‑93 (MQ=255)

                                                                            gCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGaaa             <  1:845345/93‑1 (MQ=255)

                                                                            gCAATCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGaaa             <  2:3081496/93‑1 (MQ=255)

                                                                              aaTCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACt           <  1:673835/93‑1 (MQ=255)

                                                                              aaTCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACt           <  1:2933481/93‑1 (MQ=255)

                                                                              aaTCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACt           <  2:3446475/93‑1 (MQ=255)

                                                                                tCCAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTgg         >  2:4399479/1‑93 (MQ=255)

                                                                                 ccAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGc        <  2:7116120/93‑1 (MQ=255)

                                                                                  cAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCa       <  1:7697909/93‑1 (MQ=255)

                                                                                  cAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCa       >  1:248091/1‑93 (MQ=255)

                                                                                  cAGGGCCGCGTTGCTCAGATCCGTCAGCAGATTGAACAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCa       >  1:6937765/1‑93 (MQ=255)

                                                                                   aGGGCCGCGTTGCTGAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGGAg      <  2:3263586/93‑1 (MQ=255)

                                                                                     ggCCGCGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCAggc    <  2:7200091/93‑1 (MQ=255)

AGACCTGGGTCAGGCTAAACGTGTTGTGATCAACAAAGACACCACCACTATCATCGATGGCGTGGGTGAAGAAGCTGCAATCCAGGGCCGTGTTGCTCAGATCCGTCAGCAGATTGAAGAAGCAACTTCTGACTACGACCGTGAAAAACTGCAGGAACGCGTAGCGAAACTGGCAGGCGG  >  NC_000913/4369547‑4369726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`