BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,372,457	A→G	V260A (GTG→GCG)	yjeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,372,457	0	A	G	99.5%	1270.6 / ‑4.5	402	V260A (GTG→GCG)	yjeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (196/204); total (197/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 413 total aligned reads displayed.

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       <  2:2971682/93‑1 (MQ=255)

  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       <  1:4306357/93‑1 (MQ=255)

  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       <  2:1547815/93‑1 (MQ=255)

   ttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCa                                                                                      <  2:4770141/93‑1 (MQ=255)

       cGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTa                                                                                  <  1:2571262/93‑1 (MQ=255)

        gAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTaa                                                                                 >  2:2632435/1‑93 (MQ=255)

        gAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTaa                                                                                 >  1:2335597/1‑93 (MQ=255)

         aGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAc                                                                                <  1:1409114/93‑1 (MQ=255)

          gCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACa                                                                               <  1:5668328/93‑1 (MQ=255)

           cacaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAg                                                                              <  1:7409783/93‑1 (MQ=255)

            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             >  1:378799/1‑93 (MQ=255)

             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:215643/93‑1 (MQ=255)

             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:782756/93‑1 (MQ=255)

             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:4081793/93‑1 (MQ=255)

              aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAc                                                                           <  2:7553027/93‑1 (MQ=255)

              aTGCAGGTAATACCGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAc                                                                           <  2:2578064/93‑1 (MQ=255)

               tGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACg                                                                          >  2:119276/1‑93 (MQ=255)

                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         >  1:7291989/1‑93 (MQ=255)

                  aGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctc                                                                       <  2:2582591/93‑1 (MQ=255)

                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      <  1:1003028/93‑1 (MQ=255)

                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     <  1:6622490/93‑1 (MQ=255)

                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     >  2:5522302/1‑93 (MQ=255)

                     tAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTgg                                                                    <  2:4532020/93‑1 (MQ=255)

                     tAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTgg                                                                    <  2:5930967/93‑1 (MQ=255)

                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   <  1:1841587/93‑1 (MQ=255)

                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACATAACGCTCTGGtt                                                                  <  1:136746/93‑1 (MQ=255)

                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGtt                                                                  <  1:2435265/93‑1 (MQ=255)

                        tACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTc                                                                 >  2:6406067/1‑93 (MQ=255)

                          cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAg                                                               >  1:4459546/1‑93 (MQ=255)

                           ggCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGc                                                              <  1:575441/93‑1 (MQ=255)

                           ggCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGc                                                              >  2:3844778/1‑93 (MQ=255)

                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           >  2:1042232/1‑93 (MQ=255)

                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           >  1:3696806/1‑93 (MQ=255)

                               ttACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAg                                                          >  2:1631931/1‑93 (MQ=255)

                                 tcggcGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTa                                                        <  1:803408/89‑1 (MQ=255)

                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  2:3288075/93‑1 (MQ=255)

                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  2:6111111/93‑1 (MQ=255)

                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  1:1918496/93‑1 (MQ=255)

                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  1:2901356/93‑1 (MQ=255)

                                   gCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAc                                                      <  1:359487/93‑1 (MQ=255)

                                     cGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGc                                                    <  2:6680577/93‑1 (MQ=255)

                                      ggCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGcc                                                   >  2:3745429/1‑93 (MQ=255)

                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  >  1:6274990/1‑93 (MQ=255)

                                        cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTa                                                 <  2:307007/93‑1 (MQ=255)

                                         aTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAc                                                >  2:1862074/1‑93 (MQ=255)

                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:6524486/1‑93 (MQ=255)

                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:1741873/1‑93 (MQ=255)

                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               >  1:7798583/1‑93 (MQ=255)

                                            gAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCg                                             <  1:7058585/93‑1 (MQ=255)

                                            gAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCg                                             >  1:2755824/1‑93 (MQ=255)

                                            gAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCg                                             <  1:3015238/93‑1 (MQ=255)

                                                aacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgcgc                                         >  1:534307/1‑93 (MQ=255)

                                                aacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgcgc                                         >  1:1287787/1‑93 (MQ=255)

                                                 acGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCa                                        >  1:752328/1‑93 (MQ=255)

                                                  cGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCaa                                       >  2:837423/1‑93 (MQ=255)

                                                  cGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCaa                                       >  1:4091195/1‑93 (MQ=255)

                                                   gCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAc                                      >  1:4845624/1‑93 (MQ=255)

                                                   gCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAc                                      <  1:5061534/93‑1 (MQ=255)

                                                    cATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACt                                     >  2:7145479/1‑93 (MQ=255)

                                                     aTTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACtt                                    >  1:4588345/1‑93 (MQ=255)

                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:178806/1‑93 (MQ=255)

                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:3542012/1‑93 (MQ=255)

                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               <  1:685192/93‑1 (MQ=255)

                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:2646045/93‑1 (MQ=255)

                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:2116101/93‑1 (MQ=255)

                                                           cAGGGTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:4656805/93‑1 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  1:7673836/1‑93 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:7257631/1‑93 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:7183401/1‑93 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  2:5114813/93‑1 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             >  2:1972732/1‑93 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  1:5932343/93‑1 (MQ=255)

                                                            aGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATc                             <  1:7175707/93‑1 (MQ=255)

                                                              gTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGc                           >  2:4003482/1‑93 (MQ=255)

                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          <  2:6150092/93‑1 (MQ=255)

                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          >  2:2430591/1‑93 (MQ=255)

                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      >  1:3954998/1‑93 (MQ=255)

                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      <  2:4680369/93‑1 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  1:6742983/1‑93 (MQ=255)

                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     >  1:6447444/1‑93 (MQ=255)

                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  1:4175195/93‑1 (MQ=255)

                                                                         tttGTGCGTTATCTTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                <  1:1578426/93‑1 (MQ=255)

                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  2:5386410/1‑93 (MQ=255)

                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  2:3113570/1‑93 (MQ=255)

                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  1:5485164/1‑93 (MQ=255)

                                                                          ttttgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:2722022/90‑1 (MQ=255)

                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:5306370/93‑1 (MQ=255)

                                                                           tgtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              <  1:3877200/93‑1 (MQ=255)

                                                                             tgCGTTATCGTTCTCATCACGTAACAGACCGCTATGTTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTc            <  2:4468210/93‑1 (MQ=255)

                                                                              gCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCa           <  1:6179395/93‑1 (MQ=255)

                                                                                gTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATc         <  2:1665331/93‑1 (MQ=255)

                                                                                 ttATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCt        >  1:1124699/1‑93 (MQ=255)

                                                                                  tATCGTTCGCATCACGTAACAGACCGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       <  2:4292092/93‑1 (MQ=255)

                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       >  2:4962576/1‑93 (MQ=255)

                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       >  1:3328598/1‑93 (MQ=255)

                                                                                   aTCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAc      >  2:6980325/1‑93 (MQ=255)

                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     >  2:602591/1‑93 (MQ=255)

                                                                                    tCGTCCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  1:3099655/93‑1 (MQ=255)

                                                                                     cGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCt    >  1:3544938/1‑93 (MQ=255)

                                                                                      gTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCAGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTc   <  2:7236642/93‑1 (MQ=255)

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`