BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,378,338	G→A	A520V (GCG→GTG)	frdA ←	FrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,378,338	0	G	A	98.5%	1140.3 / ‑2.0	334	A520V (GCG→GTG)	frdA	FrdA
Reads supporting (aligned to +/- strand): ref base G (1/4); new base A (133/195); total (134/200)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 339 total aligned reads displayed.

TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGC  >  NC_000913/4378248‑4378427

 tGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCa                                                                                        <  1:1038953/93‑1 (MQ=255)

 tGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCa                                                                                        >  2:2001929/1‑93 (MQ=255)

 tGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCa                                                                                        <  1:2203533/93‑1 (MQ=255)

   aGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATa                                                                                      <  2:3510674/93‑1 (MQ=255)

    ggAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATAc                                                                                     <  1:4761979/93‑1 (MQ=255)

       aGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACAtt                                                                                  >  1:4012827/1‑93 (MQ=255)

        gTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTc                                                                                 >  1:541576/1‑93 (MQ=255)

        gTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTc                                                                                 <  1:2948384/93‑1 (MQ=255)

        gTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTc                                                                                 >  1:2660081/1‑93 (MQ=255)

         ttGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCa                                                                                <  1:7282601/93‑1 (MQ=255)

         ttGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCa                                                                                <  2:2494788/93‑1 (MQ=255)

          tGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAg                                                                               <  1:2727490/93‑1 (MQ=255)

           gACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGc                                                                              <  1:5453301/93‑1 (MQ=255)

              gtcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAAc                                                                           <  1:3353739/93‑1 (MQ=255)

               tcgtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACg                                                                          >  2:7013385/1‑93 (MQ=255)

                 gtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGATTGCACCATACATTCAGCAACGtt                                                                        >  2:4335481/1‑93 (MQ=255)

                 gtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGtt                                                                        <  2:6288631/93‑1 (MQ=255)

                 gtcACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGtt                                                                        <  1:7382870/93‑1 (MQ=255)

                     cGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGa                                                                    >  2:6633364/1‑93 (MQ=255)

                     cGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGa                                                                    <  2:3685679/93‑1 (MQ=255)

                     cGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGa                                                                    <  2:7300898/93‑1 (MQ=255)

                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                   <  1:6516961/93‑1 (MQ=255)

                      gCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAc                                                                   <  2:5369854/93‑1 (MQ=255)

                       cTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAcc                                                                  <  2:1562986/93‑1 (MQ=255)

                        tCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCg                                                                 >  1:3113361/1‑93 (MQ=255)

                        tCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCg                                                                 >  1:5281057/1‑93 (MQ=255)

                         cGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGt                                                                <  1:3921733/93‑1 (MQ=255)

                         cGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGt                                                                <  2:6883539/93‑1 (MQ=255)

                          ggTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTg                                                               <  2:4997376/93‑1 (MQ=255)

                          ggTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGAACGTg                                                               >  2:3962624/1‑93 (MQ=255)

                            tGCAACCTTCGTCCAGGCGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGc                                                             <  2:5053413/93‑1 (MQ=255)

                            tGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGc                                                             >  1:6370880/1‑93 (MQ=255)

                              cAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGccc                                                           >  2:4837007/1‑93 (MQ=255)

                                 ccTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGt                                                        >  2:7537905/1‑93 (MQ=255)

                                    tCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCa                                                     <  2:3011307/93‑1 (MQ=255)

                                     cGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  1:191030/93‑1 (MQ=255)

                                     cGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  1:421384/93‑1 (MQ=255)

                                     cGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  2:4573720/93‑1 (MQ=255)

                                     cGTCCAGACGCTGGTGCGCCCCGCGGGACTCTTTACGAGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCaa                                                    <  2:6345247/93‑1 (MQ=255)

                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATg                                                  >  1:914001/1‑93 (MQ=255)

                                       tCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATg                                                  >  2:6824189/1‑93 (MQ=255)

                                        ccAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATgg                                                 <  2:4629589/93‑1 (MQ=255)

                                        ccAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATgg                                                 <  1:1960898/93‑1 (MQ=255)

                                         cAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGgt                                                <  1:5796545/93‑1 (MQ=255)

                                           gACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGgtgt                                              >  2:3718254/1‑93 (MQ=255)

                                            aCGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTa                                             <  1:1775171/93‑1 (MQ=255)

                                             cGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTag                                            <  1:7203046/93‑1 (MQ=255)

                                              gCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTaga                                           <  2:2099262/93‑1 (MQ=255)

                                               cTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGGGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGGGTagag                                          >  1:1304931/1‑93 (MQ=255)

                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  2:6682576/93‑1 (MQ=255)

                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  2:2656140/93‑1 (MQ=255)

                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  1:3538335/93‑1 (MQ=255)

                                                 ggTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCa                                        <  2:1893147/93‑1 (MQ=255)

                                                  gTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAg                                       <  1:5369301/93‑1 (MQ=255)

                                                   tGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAgg                                      <  2:5508795/93‑1 (MQ=255)

                                                   tGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAgg                                      >  1:6697180/1‑93 (MQ=255)

                                                    gcgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGt                                     <  2:7890513/93‑1 (MQ=255)

                                                     cgcgcCGCGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTc                                    <  2:3359243/93‑1 (MQ=255)

                                                       cgccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCgg                                  <  1:2273022/93‑1 (MQ=255)

                                                        gccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgt                                 <  1:6149774/93‑1 (MQ=255)

                                                         ccgcGGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtg                                >  1:763146/1‑93 (MQ=255)

                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                               <  2:7862436/93‑1 (MQ=255)

                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                               >  1:5680393/1‑93 (MQ=255)

                                                          cgcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGgtgt                               <  1:2697467/93‑1 (MQ=255)

                                                           gcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGtt                              >  1:4698533/1‑93 (MQ=255)

                                                           gcgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGtt                              >  2:2309558/1‑93 (MQ=255)

                                                            cgGGACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTg                             >  1:3323135/1‑93 (MQ=255)

                                                              ggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGaa                           <  1:2446203/93‑1 (MQ=255)

                                                              ggACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGaa                           <  2:7672755/93‑1 (MQ=255)

                                                               gACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAac                          >  1:592568/1‑93 (MQ=255)

                                                               gACTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAac                          <  1:5617928/93‑1 (MQ=255)

                                                                aCTCTTTACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAaca                         <  1:4939538/93‑1 (MQ=255)

                                                                    tttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCt                     >  1:7911972/1‑93 (MQ=255)

                                                                    tttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCt                     >  1:5524697/1‑93 (MQ=255)

                                                                     ttACGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTg                    >  2:6457777/1‑93 (MQ=255)

                                                                        cGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGaa                 <  2:4209201/93‑1 (MQ=255)

                                                                        cGTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGaa                 <  1:6916670/93‑1 (MQ=255)

                                                                         gTGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAg                >  1:5828830/1‑93 (MQ=255)

                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt               <  1:3664846/93‑1 (MQ=255)

                                                                          tGCCATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgt               <  1:5088512/93‑1 (MQ=255)

                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt             <  1:1123001/93‑1 (MQ=255)

                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt             <  2:2784312/93‑1 (MQ=255)

                                                                            ccATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAgtgt             >  1:6847849/1‑93 (MQ=255)

                                                                             cATTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTc            >  2:4246524/1‑93 (MQ=255)

                                                                              aTTGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCg           <  1:4610012/93‑1 (MQ=255)

                                                                               ttGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCgg          >  1:6754349/1‑93 (MQ=255)

                                                                               ttGCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCgg          >  1:2428590/1‑93 (MQ=255)

                                                                                 gCGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTg        <  1:2046301/93‑1 (MQ=255)

                                                                                  cGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACCCGCTGGAAGTGTCGGTGa       <  2:411018/93‑1 (MQ=255)

                                                                                  cGGAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGa       <  2:5732670/93‑1 (MQ=255)

                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  1:3723271/1‑93 (MQ=255)

                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  2:5895736/1‑93 (MQ=255)

                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  1:7671162/1‑93 (MQ=255)

                                                                                   ggAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGAt      >  1:1696742/1‑93 (MQ=255)

                                                                                    taGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATg     >  2:1267440/2‑93 (MQ=255)

                                                                                    gAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATg     >  2:2890291/1‑93 (MQ=255)

                                                                                    gAGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATg     <  2:6101426/93‑1 (MQ=255)

                                                                                     aGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgc    >  1:1463585/1‑93 (MQ=255)

                                                                                     aGTGCACCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgc    >  1:2909718/1‑93 (MQ=255)

                                                                                      gTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGATCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATgcg   <  2:1155491/93‑1 (MQ=255)

TTGAGGAAGTTGACGTCGTCACGCTCGGTGCAACCTTCGTCCAGACGCTGGTGCGCGCCGCGGGACTCTTTACGTGCCATTGCGGAGTGCGCCATACATTCAGCAACGTTCAGACCGTGGCCCAGTTCAATGGTGTAGAGCAGGTCGGTGTTGAACACGCTGGAAGTGTCGGTGATGCGC  >  NC_000913/4378248‑4378427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`