BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	526,519	T→C	C1345C (TGT→TGC)	rhsD →	RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	526,519	0	T	C	99.6%	849.5 / ‑5.4	268	C1345C (TGT→TGC)	rhsD	RhsD
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (125/142); total (125/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 277 total aligned reads displayed.

TGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGA  >  NC_000913/526429‑526608

tGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGccc                                                                                         >  1:3310707/1‑93 (MQ=255)

tGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGccc                                                                                         <  1:2246826/93‑1 (MQ=255)

 gTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGcccc                                                                                        >  2:1655342/1‑93 (MQ=255)

  tCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCca                                                                                       >  2:2784126/1‑93 (MQ=255)

   cTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCcac                                                                                      <  2:2682129/93‑1 (MQ=255)

    tGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCcaca                                                                                     >  1:1833443/1‑93 (MQ=255)

    tGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCcaca                                                                                     >  1:7276962/1‑93 (MQ=255)

    tGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCcaca                                                                                     >  2:6292328/1‑93 (MQ=255)

    tGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGACCcaca                                                                                     >  1:5217056/1‑93 (MQ=255)

     gTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAg                                                                                    <  2:4605626/93‑1 (MQ=255)

     gTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAg                                                                                    >  2:4843270/1‑93 (MQ=255)

     gTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAg                                                                                    >  2:3676712/1‑93 (MQ=255)

      tAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAgg                                                                                   >  1:2417333/1‑93 (MQ=255)

        aaGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTa                                                                                 <  1:3452027/93‑1 (MQ=255)

        aaGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTa                                                                                 <  1:365995/93‑1 (MQ=255)

          gATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACa                                                                               <  2:5764874/93‑1 (MQ=255)

           aTACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAg                                                                              >  1:4373691/1‑93 (MQ=255)

            tACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGa                                                                             >  2:4155026/1‑93 (MQ=255)

             aCTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGAt                                                                            <  1:533632/93‑1 (MQ=255)

              cTAATGGAAAATATTTTGCATCTAAGTCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAAGGTCCCACAGGTACAGATa                                                                           <  1:7650531/93‑1 (MQ=255)

              cTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATa                                                                           <  2:3375739/93‑1 (MQ=255)

              cTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATa                                                                           <  2:2495865/93‑1 (MQ=255)

                aaTGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTATGAAAAGAAAATGCCTCACAGGTACAGATaga                                                                         <  2:7508201/93‑1 (MQ=255)

                 aTGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATagag                                                                        <  2:4564223/93‑1 (MQ=255)

                  tGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGt                                                                       >  2:1044280/1‑93 (MQ=255)

                  tGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGt                                                                       >  2:5286838/1‑93 (MQ=255)

                   ggAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGtt                                                                      >  1:4714643/1‑93 (MQ=255)

                   ggAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGtt                                                                      >  1:5025060/1‑93 (MQ=255)

                     aaaaTATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTtgc                                                                    <  2:2845444/93‑1 (MQ=255)

                     aaaaTATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTtgc                                                                    <  1:6653392/93‑1 (MQ=255)

                     aaaaTATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTtgc                                                                    >  2:1266421/1‑93 (MQ=255)

                     aaaaTATATTGCATCTAAGTCAGAAACTGGTAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTtgc                                                                    <  1:5383947/93‑1 (MQ=255)

                      aaaTATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTtgct                                                                   >  1:92563/1‑93 (MQ=255)

                      aaaTATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTtgct                                                                   <  2:2246720/93‑1 (MQ=255)

                          atTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCtt                                                               >  1:1956688/1‑93 (MQ=255)

                           ttttGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTa                                                              <  1:3142689/93‑1 (MQ=255)

                            tttGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTAt                                                             >  1:4897814/1‑93 (MQ=255)

                             ttGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATc                                                            <  1:569608/93‑1 (MQ=255)

                              tGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCa                                                           <  2:7126095/93‑1 (MQ=255)

                               gCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCAt                                                          <  1:317078/93‑1 (MQ=255)

                               gCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCAt                                                          >  1:7449176/1‑93 (MQ=255)

                                cATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATa                                                         <  2:2841986/93‑1 (MQ=255)

                                  tCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACt                                                       >  2:5183124/1‑93 (MQ=255)

                                    tAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCa                                                     <  2:4949473/93‑1 (MQ=255)

                                     aaGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCAc                                                    <  2:2340548/93‑1 (MQ=255)

                                      aGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACg                                                   <  1:7797326/93‑1 (MQ=255)

                                       ggCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACgg                                                  >  1:3252620/1‑93 (MQ=255)

                                       ggCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACgg                                                  >  2:3119246/1‑93 (MQ=255)

                                          aGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGc                                               >  1:7185241/1‑93 (MQ=255)

                                          aGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGc                                               >  1:4707687/1‑93 (MQ=255)

                                          aGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGc                                               <  2:3242974/93‑1 (MQ=255)

                                           gAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCa                                              >  1:7827507/1‑93 (MQ=255)

                                                tGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAg                                         >  1:2148597/1‑93 (MQ=255)

                                                  aTAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTc                                       <  1:3524130/93‑1 (MQ=255)

                                                   tAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCa                                      <  1:1273583/93‑1 (MQ=255)

                                                   tAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCa                                      <  2:4667236/93‑1 (MQ=255)

                                                   tAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCa                                      >  2:4255661/1‑93 (MQ=255)

                                                    aaTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCAt                                     <  1:1423364/93‑1 (MQ=255)

                                                    aaTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCAt                                     <  1:2347064/93‑1 (MQ=255)

                                                     aTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATg                                    <  1:4532953/93‑1 (MQ=255)

                                                     aTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATg                                    >  2:1023197/1‑93 (MQ=255)

                                                     aTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATg                                    <  1:2760234/93‑1 (MQ=255)

                                                     aTTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATg                                    <  2:6535750/93‑1 (MQ=255)

                                                       ttAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGc                                  <  1:6400743/93‑1 (MQ=255)

                                                        tAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCg                                 >  2:7849571/1‑93 (MQ=255)

                                                        tAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCg                                 <  2:1131064/93‑1 (MQ=255)

                                                         aaGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGa                                <  1:401672/93‑1 (MQ=255)

                                                         aaGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGa                                >  2:586120/1‑93 (MQ=255)

                                                          aGAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGAt                               <  2:6078937/93‑1 (MQ=255)

                                                           gAAAGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGAtt                              <  1:4110046/93‑1 (MQ=255)

                                                            aaaGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTa                             <  1:4816426/93‑1 (MQ=255)

                                                            aaaGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTa                             <  2:7589516/93‑1 (MQ=255)

                                                              aGGAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATg                           <  1:1751893/93‑1 (MQ=255)

                                                                gAGTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGtt                         <  2:5714938/93‑1 (MQ=255)

                                                                 aGTCGTATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtg                        <  2:3515910/93‑1 (MQ=255)

                                                                  gTCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtga                       <  1:2865497/93‑1 (MQ=255)

                                                                   tCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtgat                      >  2:6870061/1‑93 (MQ=255)

                                                                   tCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtgat                      <  2:4387512/93‑1 (MQ=255)

                                                                   tCATATCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtgat                      >  1:3900374/1‑93 (MQ=255)

                                                                     atatCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtgatga                    <  2:1971547/93‑1 (MQ=255)

                                                                     atatCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtgatga                    <  2:1475162/93‑1 (MQ=255)

                                                                      tatCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGaa                   >  1:3616507/1‑93 (MQ=255)

                                                                       atCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAAt                  >  1:754578/1‑93 (MQ=255)

                                                                       atCCTCTGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAAt                  <  2:3408976/93‑1 (MQ=255)

                                                                          ctctGAAAAGAAAATGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAAtttt               <  1:5158698/93‑1 (MQ=255)

                                                                              gaaaagaaaaTGCCCCACAGGTACAGGTAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTc           <  1:6467437/93‑1 (MQ=255)

                                                                              gaaaagaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTc           >  1:5981769/1‑93 (MQ=255)

                                                                               aaaagaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCa          <  1:6742141/93‑1 (MQ=255)

                                                                                 aagaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAg        <  1:6562580/93‑1 (MQ=255)

                                                                                 aagaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAg        <  2:6752723/93‑1 (MQ=255)

                                                                                 aagaaaaGGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAg        <  2:2025138/93‑1 (MQ=255)

                                                                                  agaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGt       >  2:5734149/1‑93 (MQ=255)

                                                                                  agaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGt       <  1:5515578/93‑1 (MQ=255)

                                                                                  agaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGt       <  2:4483171/93‑1 (MQ=255)

                                                                                  agaaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGt       <  1:1083498/93‑1 (MQ=255)

                                                                                    aaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAg     <  1:2654340/93‑1 (MQ=255)

                                                                                    aaaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAg     <  2:7780828/93‑1 (MQ=255)

                                                                                     aaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGc    <  2:3821734/93‑1 (MQ=255)

                                                                                     aaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGc    <  2:3896945/93‑1 (MQ=255)

                                                                                     aaaTGCCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGc    <  1:3852995/93‑1 (MQ=255)

TGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGA  >  NC_000913/526429‑526608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`