BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,415,130	C→T	P201S (CCT→TCT)	yjfP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,415,130	0	C	T	99.2%	1280.6 / ‑4.1	361	P201S (CCT→TCT)	yjfP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (185/173); total (187/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 395 total aligned reads displayed.

CCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGT  >  NC_000913/4415040‑4415219

ccACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtct                                                                                         >  2:442005/1‑93 (MQ=255)

 cACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtctg                                                                                        >  1:4510656/1‑93 (MQ=255)

  aCTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtctgc                                                                                       >  2:6078842/1‑93 (MQ=255)

  aCTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtctgc                                                                                       <  2:4365068/93‑1 (MQ=255)

  aCTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtctgc                                                                                       <  2:3922738/93‑1 (MQ=255)

   cTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtctgcc                                                                                      >  2:1396360/1‑93 (MQ=255)

   cTGGCAGAGTGGGAAGCGACAAACCACCAGGAACAACTTAGTGACAGTCCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGtgtctgcc                                                                                      <  1:1877292/93‑1 (MQ=255)

    tGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCcg                                                                                     <  1:1052121/93‑1 (MQ=255)

     ggCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCcga                                                                                    <  2:3480272/93‑1 (MQ=255)

     ggCAGAGGGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCcga                                                                                    <  2:1737851/93‑1 (MQ=255)

      gCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCcgac                                                                                   >  1:5792464/1‑93 (MQ=255)

      gCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCcgac                                                                                   <  1:3050284/93‑1 (MQ=255)

         gagTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGaa                                                                                >  1:4441882/1‑93 (MQ=255)

         gagTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGaa                                                                                >  1:6527124/1‑93 (MQ=255)

         gagTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGaa                                                                                <  1:703919/93‑1 (MQ=255)

         gagTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGaa                                                                                >  2:495477/1‑93 (MQ=255)

           gTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATc                                                                              >  2:1963205/1‑93 (MQ=255)

           gTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATc                                                                              >  1:5012692/1‑93 (MQ=255)

            tGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCa                                                                             >  2:1524446/1‑93 (MQ=255)

            tGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCa                                                                             >  2:2438024/1‑93 (MQ=255)

            tGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCa                                                                             >  2:2926273/1‑93 (MQ=255)

            tGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCa                                                                             >  1:1261051/1‑93 (MQ=255)

             gggAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCAc                                                                            >  2:2554140/1‑93 (MQ=255)

              ggAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACt                                                                           <  1:4232111/93‑1 (MQ=255)

               gAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTa                                                                          <  2:911954/93‑1 (MQ=255)

               gAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTa                                                                          <  2:2163727/93‑1 (MQ=255)

               gAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTa                                                                          <  1:7699618/93‑1 (MQ=255)

                 aGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACg                                                                        >  2:3682826/1‑93 (MQ=255)

                   cGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGtt                                                                      <  1:6395905/93‑1 (MQ=255)

                      cAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTgc                                                                   <  2:7351199/93‑1 (MQ=255)

                       aaaCCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTgca                                                                  <  1:6634365/93‑1 (MQ=255)

                          ccaccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTgcagca                                                               <  1:255546/93‑1 (MQ=255)

                            cccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAgg                                                             <  2:2292277/92‑1 (MQ=255)

                            cccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAgg                                                             <  2:3828136/92‑1 (MQ=255)

                            accTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAgg                                                             >  2:2820530/1‑93 (MQ=255)

                            accTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAgg                                                             >  2:3388252/1‑93 (MQ=255)

                             ccTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTGGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGc                                                            <  2:2205286/93‑1 (MQ=255)

                               tGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCt                                                          <  1:7584285/93‑1 (MQ=255)

                                ggAACAACTTGGTGACAGACCTCTACTGCTGTGGCATGGACTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCCGGCCtt                                                         >  2:6423425/1‑93 (MQ=255)

                                ggAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCtt                                                         <  2:3016126/93‑1 (MQ=255)

                                  aacaacGTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTaa                                                       <  1:3776031/93‑1 (MQ=255)

                                   acaacTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGCGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAg                                                      <  1:1593969/93‑1 (MQ=255)

                                     aacTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCg                                                    >  1:2365370/1‑93 (MQ=255)

                                            tGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTATGTTTGCAGCAGGCCTTAAgcggggcggg                                             >  2:1596764/1‑93 (MQ=255)

                                            tGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggg                                             >  2:7076647/1‑93 (MQ=255)

                                             gACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggga                                            <  2:7524034/93‑1 (MQ=255)

                                             gACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggga                                            <  2:4603529/93‑1 (MQ=255)

                                             gACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggga                                            <  2:5348556/93‑1 (MQ=255)

                                              aCAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacgggac                                           <  2:2673982/93‑1 (MQ=255)

                                                  aCCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGAcgggacggga                                       <  1:7289453/93‑1 (MQ=255)

                                                   ccTCTACTGCTGTGGCATGGCCTCGACTATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGAt                                      <  1:4100754/93‑1 (MQ=255)

                                                    ctctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATa                                     <  2:4028730/93‑1 (MQ=255)

                                                    ctctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATa                                     <  2:4709495/93‑1 (MQ=255)

                                                    ctctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATa                                     >  1:5850871/1‑93 (MQ=255)

                                                     tctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATaa                                    >  2:1086930/1‑93 (MQ=255)

                                                     tctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATaa                                    >  1:2628925/1‑93 (MQ=255)

                                                     tctACTGCTGTGGCATGGCCTCGACGATGAAGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATaa                                    >  1:5335464/1‑93 (MQ=255)

                                                      ctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTGTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATaaa                                   >  1:6177492/1‑93 (MQ=255)

                                                      ctACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATaaa                                   <  1:2748042/93‑1 (MQ=255)

                                                       tACTGTTGTGGCATGGCTTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                  <  2:2032347/93‑1 (MQ=255)

                                                       tACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                  >  1:5591276/1‑93 (MQ=255)

                                                       tACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                  >  2:5698486/1‑93 (MQ=255)

                                                       tACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                  >  1:7827154/1‑93 (MQ=255)

                                                       tACTGCTGTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAAc                                  >  2:181662/1‑93 (MQ=255)

                                                         ctgctgTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTg                                >  1:4843144/1‑93 (MQ=255)

                                                         ctgctgTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTg                                >  1:3306653/1‑93 (MQ=255)

                                                          tgctgTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGc                               >  2:7787651/1‑93 (MQ=255)

                                                           gctgTGGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCt                              >  2:651303/1‑93 (MQ=255)

                                                              gtgGCATGGCATAGAAGATGACGTTGTGTCTGCCGACGAATCACTACATTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAAc                           <  2:7014072/93‑1 (MQ=255)

                                                               tgGCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAAcc                          >  1:1684566/1‑93 (MQ=255)

                                                                ggCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAActt                         >  1:1431962/1‑91 (MQ=255)

                                                                ggCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCt                         <  1:3477605/93‑1 (MQ=255)

                                                                ggCATGGCCTCGAAGATGACGTTGTGTCTGCCGACGAATTACTACGTTTGCAACAGGCCTTAAGCGAGACGGGGCGGGATAAAGCGCTCACCt                         >  2:342095/1‑93 (MQ=255)

                                                                 gCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGTATCAGGCCTTAAGCGAAACGGGACGGGATAAACTGCTAACTTg                        >  2:6738193/1‑93 (MQ=255)

                                                                 gCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACTTg                        <  1:3157868/93‑1 (MQ=255)

                                                                 gCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTg                        >  1:4588319/1‑93 (MQ=255)

                                                                 gCATGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTg                        >  1:4668027/1‑93 (MQ=255)

                                                                    tGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTc                     >  2:7123645/1‑93 (MQ=255)

                                                                    tGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTc                     >  1:4019105/1‑93 (MQ=255)

                                                                    tGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTc                     >  1:6775863/1‑93 (MQ=255)

                                                                    tGGCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTc                     >  2:5833930/1‑93 (MQ=255)

                                                                     ggCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCa                    <  1:2224749/93‑1 (MQ=255)

                                                                     ggCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCa                    <  1:5167874/93‑1 (MQ=255)

                                                                      gCCTCGACGATGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCAt                   >  2:4860602/1‑93 (MQ=255)

                                                                          cgacgaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACTTGTTCATGGCa               <  2:5942684/93‑1 (MQ=255)

                                                                          cgacgaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCa               <  2:5460271/93‑1 (MQ=255)

                                                                            acgaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGc             <  1:2067362/93‑1 (MQ=255)

                                                                            acgaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGc             >  1:2164946/1‑93 (MQ=255)

                                                                             cgaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGcc            <  2:447007/93‑1 (MQ=255)

                                                                              gaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGccc           >  1:815963/1‑92 (MQ=255)

                                                                              gaTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCa           <  2:2534441/93‑1 (MQ=255)

                                                                               aTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAg          <  2:7678210/93‑1 (MQ=255)

                                                                               aTGACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAg          <  1:3586943/93‑1 (MQ=255)

                                                                                 gaggTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgc        <  2:6913820/90‑1 (MQ=255)

                                                                                 gACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACTTGTTCATGGCAGCCAGgc        >  1:6755730/1‑93 (MQ=255)

                                                                                 gACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCCGCCAGgc        >  1:4303765/1‑93 (MQ=255)

                                                                                 gACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAggg        >  2:4679893/1‑92 (MQ=255)

                                                                                 gACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgc        >  1:2950829/1‑93 (MQ=255)

                                                                                 gACGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgc        >  2:3281373/1‑93 (MQ=255)

                                                                                  aCGTTGTGTCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgcg       <  1:5938557/93‑1 (MQ=255)

CCACTGGCAGAGTGGGAAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTGCTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGT  >  NC_000913/4415040‑4415219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`