BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,211	T→C	A53A (GCA→GCG)	yjfY ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,211	0	T	C	99.8%	1247.8 / ‑5.4	398	A53A (GCA→GCG)	yjfY	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (193/204); total (193/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 409 total aligned reads displayed.

CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT  >  NC_000913/4422121‑4422300

  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       >  1:4263063/1‑93 (MQ=255)

  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       >  2:4409427/1‑93 (MQ=255)

  tatTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCtt                                                                                       >  1:705076/1‑93 (MQ=255)

   atTGCCGTTACTCCCCGGTTCCCTCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTc                                                                                      <  2:4295236/93‑1 (MQ=255)

    ttGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCg                                                                                     >  1:1857976/1‑93 (MQ=255)

     tGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGc                                                                                    >  1:5310161/1‑93 (MQ=255)

      gCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCt                                                                                   <  2:4922485/93‑1 (MQ=255)

        cGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCttt                                                                                 <  1:3376534/93‑1 (MQ=255)

        cGTTACTCCCCGGTCCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCttt                                                                                 <  2:6024769/93‑1 (MQ=255)

         gTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTc                                                                                <  1:4524571/93‑1 (MQ=255)

           tACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAg                                                                              <  2:5333463/93‑1 (MQ=255)

           tACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAg                                                                              <  2:2112868/93‑1 (MQ=255)

           tACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAg                                                                              >  1:1407946/1‑93 (MQ=255)

           tACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCACCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAg                                                                              <  2:7016673/93‑1 (MQ=255)

            aCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGt                                                                             >  2:1202164/1‑93 (MQ=255)

            aCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGt                                                                             <  2:3444531/93‑1 (MQ=255)

               ccccGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGc                                                                          <  1:2522654/93‑1 (MQ=255)

               ccccGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGc                                                                          <  2:5581935/93‑1 (MQ=255)

                cccGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCg                                                                         >  2:1475633/1‑93 (MQ=255)

                   ggTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGaaa                                                                      <  2:3945173/93‑1 (MQ=255)

                   ggTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGaaa                                                                      <  1:352619/93‑1 (MQ=255)

                   ggTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGaaa                                                                      <  2:6538376/93‑1 (MQ=255)

                     ttCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAtt                                                                    >  1:2199512/1‑93 (MQ=255)

                      tCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTa                                                                   >  1:2701064/1‑93 (MQ=255)

                      tCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAAATa                                                                   <  1:1336052/93‑1 (MQ=255)

                       cccGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAt                                                                  <  1:6239831/93‑1 (MQ=255)

                        ccGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtg                                                                 >  1:5470272/1‑93 (MQ=255)

                        ccGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtg                                                                 >  1:2941351/1‑93 (MQ=255)

                          gCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgat                                                               >  2:7574263/1‑93 (MQ=255)

                            aTCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattg                                                             <  2:866032/93‑1 (MQ=255)

                            aTCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattg                                                             <  1:1144842/93‑1 (MQ=255)

                             tCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattga                                                            >  2:5034447/1‑93 (MQ=255)

                             tCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattga                                                            >  1:179332/1‑93 (MQ=255)

                              cAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTAtgattgat                                                           <  2:6920258/93‑1 (MQ=255)

                                gAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGatat                                                         <  1:1042151/93‑1 (MQ=255)

                                gAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGACTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGatat                                                         >  1:4087340/1‑93 (MQ=255)

                                  aTTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaa                                                       >  1:4039919/1‑93 (MQ=255)

                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      >  1:3249799/1‑93 (MQ=255)

                                   ttACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa                                                      <  1:7540394/93‑1 (MQ=255)

                                    tACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                     <  2:3702572/93‑1 (MQ=255)

                                    tACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt                                                     <  2:1654286/93‑1 (MQ=255)

                                     aCGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc                                                    <  1:6593870/93‑1 (MQ=255)

                                     aCGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc                                                    <  2:7776192/93‑1 (MQ=255)

                                       gTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc                                                  <  1:6466194/93‑1 (MQ=255)

                                        tGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACg                                                 <  2:1094835/93‑1 (MQ=255)

                                         ggTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                                >  1:4609218/1‑93 (MQ=255)

                                         ggTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc                                                <  1:2453784/93‑1 (MQ=255)

                                           tagtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCt                                              <  1:5666580/93‑1 (MQ=255)

                                             gtaCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTaa                                            <  2:3503003/93‑1 (MQ=255)

                                                 gTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCtt                                        <  2:6859393/93‑1 (MQ=255)

                                                  ttGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCttt                                       >  1:3050396/1‑93 (MQ=255)

                                                   tGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTg                                      <  1:6354881/93‑1 (MQ=255)

                                                    gcgcCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGc                                     <  1:942754/93‑1 (MQ=255)

                                                     cgcCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGca                                    >  1:6995114/1‑93 (MQ=255)

                                                       ccTTGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGcaca                                  <  2:2791204/93‑1 (MQ=255)

                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                >  2:4693309/1‑93 (MQ=255)

                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                >  2:1985821/1‑93 (MQ=255)

                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                >  2:2231973/1‑93 (MQ=255)

                                                         ttGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATc                                >  2:5889440/1‑93 (MQ=255)

                                                          tGCGCATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCg                               >  1:6865226/1‑93 (MQ=255)

                                                           gcgcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGt                              >  2:7462079/1‑93 (MQ=255)

                                                             gcATCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTcc                            >  2:5191492/1‑93 (MQ=255)

                                                             gcATCTGTTTCTTCATTTAATGCCTGACGCGCTGCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTcc                            <  1:7632720/93‑1 (MQ=255)

                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         >  1:3778112/1‑93 (MQ=255)

                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         >  2:1356812/1‑93 (MQ=255)

                                                                tCTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCata                         <  1:4891522/93‑1 (MQ=255)

                                                                 cTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCatat                        <  2:6378764/93‑1 (MQ=255)

                                                                 cTGTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCatat                        >  1:1573599/1‑93 (MQ=255)

                                                                   gTTTCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATAttt                      >  2:1787426/1‑93 (MQ=255)

                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCGCATCGTCCATATTTc                     >  2:2546598/1‑93 (MQ=255)

                                                                    tttCTTCATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTc                     <  1:3887714/93‑1 (MQ=255)

                                                                     ttcttcATTTGATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCt                    <  2:7220602/93‑1 (MQ=255)

                                                                      tcttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTg                   <  1:2032597/93‑1 (MQ=255)

                                                                        ttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGc                 <  1:86909/93‑1 (MQ=255)

                                                                        ttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGc                 <  2:7898475/93‑1 (MQ=255)

                                                                        ttcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGc                 >  1:6908361/1‑93 (MQ=255)

                                                                         tcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGcc                <  1:6754210/93‑1 (MQ=255)

                                                                         tcATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGcc                <  2:2308500/93‑1 (MQ=255)

                                                                          cATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCt               <  2:5418383/93‑1 (MQ=255)

                                                                          cATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCt               <  2:3235712/93‑1 (MQ=255)

                                                                          cATTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCt               <  1:4728353/93‑1 (MQ=255)

                                                                           aTTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtg              <  2:7721362/93‑1 (MQ=255)

                                                                           aTTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtg              <  1:6117028/93‑1 (MQ=255)

                                                                           aTTTAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtg              <  2:7556034/93‑1 (MQ=255)

                                                                             ttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggt            <  2:2918624/93‑1 (MQ=255)

                                                                             ttAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggg            >  1:7429794/1‑92 (MQ=255)

                                                                              tAATGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtg           <  2:415314/93‑1 (MQ=255)

                                                                               aaTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtgg          >  2:5992192/1‑93 (MQ=255)

                                                                               aaTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtgg          <  1:3631605/93‑1 (MQ=255)

                                                                               aaTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtgg          >  1:7759751/1‑93 (MQ=255)

                                                                               aaTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCtggtgg          <  2:1865434/93‑1 (MQ=255)

                                                                                aTGCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTggtggt         <  1:5006560/93‑1 (MQ=255)

                                                                                  gCCTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttt       >  1:1116496/1‑92 (MQ=255)

                                                                                    cTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt     >  1:4328832/1‑93 (MQ=255)

                                                                                    cTGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatt     <  2:5111974/93‑1 (MQ=255)

                                                                                     tGACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGttatta    <  2:6099456/93‑1 (MQ=255)

                                                                                      gACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCTCGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTaa   <  1:5377063/93‑1 (MQ=255)

                                                                                      gACGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTaa   <  2:4049356/93‑1 (MQ=255)

                                                                                       aCGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAt  >  2:5795932/1‑93 (MQ=255)

                                                                                       aCGCGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAt  <  1:5895363/93‑1 (MQ=255)

CATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAAT  >  NC_000913/4422121‑4422300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`