BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,431,142	T→C	H154R (CAC→CGC)	ytfG ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,431,142	0	T	C	99.5%	1109.4 / ‑4.2	362	H154R (CAC→CGC)	ytfG	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (160/199); total (163/199)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 380 total aligned reads displayed.

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGC  >  NC_000913/4431052‑4431231

tCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCgcgt                                                                                         >  1:454689/1‑93 (MQ=255)

   cTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTCAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTcc                                                                                      <  2:3090604/93‑1 (MQ=255)

    tAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCa                                                                                     <  2:1662700/93‑1 (MQ=255)

     aaTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAg                                                                                    <  1:7081428/93‑1 (MQ=255)

     aaTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAg                                                                                    <  2:3323683/93‑1 (MQ=255)

      aTCCCGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGt                                                                                   <  1:2578747/93‑1 (MQ=255)

      aTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGt                                                                                   <  2:4069748/93‑1 (MQ=255)

      aTCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGt                                                                                   <  2:207082/93‑1 (MQ=255)

       tCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtg                                                                                  <  1:6247106/93‑1 (MQ=255)

        cACGCGTGCCGCAGCTGCCGCATAATCTGCCGGGGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgc                                                                                 <  2:6316467/93‑1 (MQ=255)

         ccgcgTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgct                                                                                <  1:2375078/92‑1 (MQ=255)

           gcgTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGtgctgc                                                                              >  2:6790291/1‑93 (MQ=255)

            cgTGCCCGAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGcc                                                                             <  1:414455/93‑1 (MQ=255)

             gTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCg                                                                            <  1:3019135/93‑1 (MQ=255)

              tGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgg                                                                           <  2:5372895/93‑1 (MQ=255)

              tGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCgg                                                                           <  2:3878475/93‑1 (MQ=255)

               gCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCggg                                                                          <  2:6846608/93‑1 (MQ=255)

                  gCAGCTGCCGCATAATCTGCCCGAGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcg                                                                       <  2:4300422/93‑1 (MQ=255)

                   cAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgc                                                                      >  1:7757859/1‑93 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGgcgcg                                                                     >  1:3197847/1‑92 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     >  1:1329944/1‑93 (MQ=255)

                    aGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCt                                                                     <  1:1093107/93‑1 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    <  2:2706651/93‑1 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    <  2:5724056/93‑1 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    <  1:589987/93‑1 (MQ=255)

                     gCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGATCCAGTGCTGCCGGGGAGCTg                                                                    >  2:1246746/1‑93 (MQ=255)

                     gCTGCCGCATAAGCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTg                                                                    <  1:90539/93‑1 (MQ=255)

                      cTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTgg                                                                   <  2:6687312/93‑1 (MQ=255)

                      cTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTgg                                                                   <  2:2907191/93‑1 (MQ=255)

                         ccGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCgg                                                                >  2:766826/1‑92 (MQ=255)

                         ccGCATAATCTGCCCGCGTTGCTGAGCCGATTTTGACATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGa                                                                <  1:4005424/93‑1 (MQ=255)

                          cGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAg                                                               <  2:5519447/93‑1 (MQ=255)

                          cGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAg                                                               >  1:4786977/1‑93 (MQ=255)

                          cGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAg                                                               >  1:3615776/1‑93 (MQ=255)

                           gCATAATCTGCCCGCGTTGCTGAGGCGATTTTTCCATATCCTGCCCCACCGATAAATACGGCGCGCTCCAGTGCTGCCGGCGGGCTGGCGAgg                                                              >  2:4121282/1‑93 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGTCGCTGGCGAGGt                                                             <  2:3538194/93‑1 (MQ=255)

                            cATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGt                                                             >  1:2688306/1‑93 (MQ=255)

                             aTAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTa                                                            >  2:2591400/1‑93 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           >  1:6084668/1‑93 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           >  2:5595397/1‑93 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAg                                                           <  2:3144157/93‑1 (MQ=255)

                              tAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGCCGCTGGCGAGGTAg                                                           >  1:3816954/1‑93 (MQ=255)

                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                         >  1:1730541/1‑93 (MQ=255)

                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                         <  1:6485383/93‑1 (MQ=255)

                                aTCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtt                                                         >  2:5693732/1‑93 (MQ=255)

                                 tCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCCCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGttt                                                        <  2:6210399/93‑1 (MQ=255)

                                 tCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGttt                                                        >  1:7592577/1‑93 (MQ=255)

                                 tCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGttt                                                        >  2:5868004/1‑93 (MQ=255)

                                  cTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtttt                                                       <  1:4747945/93‑1 (MQ=255)

                                  cTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGtttt                                                       >  1:2945046/1‑93 (MQ=255)

                                   tGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTc                                                      <  2:3685389/93‑1 (MQ=255)

                                      ccGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCt                                                   >  2:4731385/1‑93 (MQ=255)

                                      ccGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCt                                                   <  1:7331013/93‑1 (MQ=255)

                                           ttGCTGAGGCGTTGTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                              <  2:2279672/93‑1 (MQ=255)

                                           ttGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                              <  2:829952/93‑1 (MQ=255)

                                           ttGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                              <  1:4921397/93‑1 (MQ=255)

                                           ttGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTAcc                                              <  2:1221915/93‑1 (MQ=255)

                                            tGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCa                                             <  1:683785/93‑1 (MQ=255)

                                             gCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAg                                            >  1:5931312/1‑93 (MQ=255)

                                               tGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGcc                                          >  2:1061225/1‑93 (MQ=255)

                                               tGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGcc                                          >  2:7150678/1‑93 (MQ=255)

                                                gAGGCTATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                         <  2:3744823/93‑1 (MQ=255)

                                                gAGGCGATTTTGCCATCGCCCCCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCg                                         >  2:1890858/1‑93 (MQ=255)

                                                 gggCGATTTTGCCACCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                        <  1:6640884/92‑1 (MQ=255)

                                                 aGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                        >  2:1505340/1‑93 (MQ=255)

                                                 aGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGt                                        >  2:2447382/1‑93 (MQ=255)

                                                  ggCGTTTTTGCCATCCCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGtt                                       <  2:3077308/93‑1 (MQ=255)

                                                      aTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgc                                   >  1:6305754/1‑93 (MQ=255)

                                                      aTTTTGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTgcgc                                   >  2:7492108/1‑93 (MQ=255)

                                                         ttGCCATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCgcagc                                <  1:7490496/93‑1 (MQ=255)

                                                           gCCACCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGcagcag                              <  2:664734/93‑1 (MQ=255)

                                                            ccATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAgg                             <  1:2231002/93‑1 (MQ=255)

                                                             cATCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAggg                            <  1:1973540/93‑1 (MQ=255)

                                                              aTCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgt                           >  2:4586703/1‑93 (MQ=255)

                                                              aTCGCCCGCCGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgt                           >  2:7757228/1‑93 (MQ=255)

                                                                cgcccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgtgt                         <  1:851753/93‑1 (MQ=255)

                                                                cgcccgccGCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGgggt                         >  2:3002281/1‑93 (MQ=255)

                                                                   ccgccgCACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTaaa                      <  1:1027309/93‑1 (MQ=255)

                                                                   ccgccgCACCGATAAACACGCCGCGTCCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTaaa                      <  2:3999233/93‑1 (MQ=255)

                                                                    cgccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAc                     <  2:5081046/93‑1 (MQ=255)

                                                                    cgccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAAc                     <  2:2303995/93‑1 (MQ=255)

                                                                     gccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGCTGCGCAGCAGGGTGTAAACg                    >  2:6977782/1‑93 (MQ=255)

                                                                      ccgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGa                   <  1:3445552/93‑1 (MQ=255)

                                                                      ccgcACCGAGAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGa                   <  1:5376529/93‑1 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  >  1:5673650/1‑93 (MQ=255)

                                                                       cgcACCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGAt                  >  1:7154592/1‑93 (MQ=255)

                                                                          aCCGATAAATCCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               <  1:1469813/93‑1 (MQ=255)

                                                                          aCCGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGcc               <  2:709138/93‑1 (MQ=255)

                                                                           ccGATAAATCCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa              <  2:1594064/93‑1 (MQ=255)

                                                                           ccGATAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCa              >  1:498782/1‑93 (MQ=255)

                                                                               tAAATACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAAt          >  1:3863711/1‑93 (MQ=255)

                                                                               tAAACACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTCTTCGCCGTACCAGCCGCTGCGCAGCAGGGTGGAAACGACGCCAGaac          >  1:6034338/1‑92 (MQ=255)

                                                                                aaaTACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATc         <  2:7924869/93‑1 (MQ=255)

                                                                                   tACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagc      >  1:6938888/1‑93 (MQ=255)

                                                                                   tACGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagc      <  2:1801404/93‑1 (MQ=255)

                                                                                    aCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagcc     <  2:6484811/93‑1 (MQ=255)

                                                                                    aCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagcc     >  2:4434117/1‑93 (MQ=255)

                                                                                    aCGCCGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTATCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagcc     >  2:4834856/1‑93 (MQ=255)

                                                                                     cgccgcGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagcca    <  2:2647230/93‑1 (MQ=255)

                                                                                       ccGCGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATcagccagc  >  2:6412048/1‑93 (MQ=255)

TCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGC  >  NC_000913/4431052‑4431231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`