BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,434,828	T→C	Y166H (TAT→CAT)	cysQ →	CysQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,434,828	0	T	C	99.5%	1184.7 / ‑5.4	376	Y166H (TAT→CAT)	cysQ	CysQ
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (160/213); total (161/215)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

Only 100 of 382 total aligned reads displayed.

TGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTAC  >  NC_000913/4434738‑4434917

gggggTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCAt                                                                                         <  2:6075374/90‑1 (MQ=255)

ggCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCAt                                                                                         <  2:6749081/92‑1 (MQ=255)

ggCGGTGGCCGCAAGAAAATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCAt                                                                                         <  2:5800096/92‑1 (MQ=255)

 gCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATc                                                                                        >  2:7853190/1‑93 (MQ=255)

  cGGTGTGCGCAAGCAGATTCAGTTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCt                                                                                       <  1:820700/93‑1 (MQ=255)

  cGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCt                                                                                       >  2:5522984/1‑93 (MQ=255)

  cGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCt                                                                                       <  1:6263007/93‑1 (MQ=255)

   ggTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTg                                                                                      >  2:291286/1‑93 (MQ=255)

    gtgtGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGc                                                                                     >  2:3560617/1‑93 (MQ=255)

    gtgtGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGc                                                                                     <  1:490973/93‑1 (MQ=255)

      gtgCGCAAGCAGATTCAGGTCCGCGATGCGCCCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCaa                                                                                   <  1:134510/93‑1 (MQ=255)

        gcgcAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCACGCGGATGCGGAGCTGAAAGAGCATCTGCAACa                                                                                 <  2:6583999/93‑1 (MQ=255)

         cgcAAGCAGATTCGGGTCCGCGACGCGCCCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAg                                                                                <  2:649981/93‑1 (MQ=255)

         cccAAGCAGATTCGGGTCCGCGACGCGCCCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAg                                                                                <  2:2129287/91‑1 (MQ=255)

          gcAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGc                                                                               >  1:3429402/1‑93 (MQ=255)

          gcAAGCAGATTCAGGCCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGc                                                                               <  2:1517647/93‑1 (MQ=255)

           cAAGAAAATTCGGGCGCGCGATGCCCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCt                                                                              <  1:3885824/93‑1 (MQ=255)

            acgtaGATTCAGGCCCGCGAGCCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCtt                                                                             <  2:7317796/89‑1 (MQ=255)

            aaGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCtt                                                                             >  1:2290485/1‑93 (MQ=255)

             aGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTg                                                                            <  1:7568924/93‑1 (MQ=255)

              gCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTgg                                                                           >  1:2907847/1‑93 (MQ=255)

               cAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGc                                                                          >  2:4737351/1‑93 (MQ=255)

                aGATTCAGGCCCGGGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCg                                                                         <  1:780757/93‑1 (MQ=255)

                 gATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGa                                                                        <  2:536524/93‑1 (MQ=255)

                 gATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGa                                                                        >  1:3528903/1‑93 (MQ=255)

                  aTTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGaa                                                                       >  2:5477930/1‑93 (MQ=255)

                   ttCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAAc                                                                      >  2:2797429/1‑93 (MQ=255)

                   ttCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAAc                                                                      >  2:919752/1‑93 (MQ=255)

                    tcgggTCCGCGCTGCCCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACa                                                                     <  2:3192209/90‑1 (MQ=255)

                       ggTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCa                                                                  <  1:2089380/93‑1 (MQ=255)

                        gTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAg                                                                 <  2:7189097/93‑1 (MQ=255)

                          ccGGGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGAc                                                               <  1:1818894/93‑1 (MQ=255)

                          ccGCGATGCGCCCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGAc                                                               <  1:5648765/93‑1 (MQ=255)

                            gcgcTGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCa                                                             <  2:4481870/93‑1 (MQ=255)

                            gcgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCa                                                             >  2:7834216/1‑93 (MQ=255)

                             cgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCAc                                                            <  2:6446483/93‑1 (MQ=255)

                             cgATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCAc                                                            <  2:2592754/93‑1 (MQ=255)

                              gATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACg                                                           >  1:3132942/1‑93 (MQ=255)

                                tGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTc                                                         <  1:6890895/93‑1 (MQ=255)

                                     gcccgccGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCg                                                    <  1:1025019/93‑1 (MQ=255)

                                     gcccgccGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCg                                                    >  2:2312526/1‑93 (MQ=255)

                                     gcccgccGCTGGTGGTGATCAGCCGATCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCACCAGCTTGGCGAACATCAGACCACGTACATCg                                                    >  1:7745659/1‑93 (MQ=255)

                                     ccccgccGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCg                                                    <  2:1944420/92‑1 (MQ=255)

                                     ccccgccGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCg                                                    <  2:4889113/92‑1 (MQ=255)

                                           cgcTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCtt                                              >  2:5028522/1‑93 (MQ=255)

                                            gcTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTc                                             <  1:4848505/93‑1 (MQ=255)

                                            gcTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTc                                             <  1:282486/93‑1 (MQ=255)

                                             cTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGCACATCTGCTCTTCg                                            >  1:4554134/1‑93 (MQ=255)

                                              tggtggTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGc                                           >  2:6160509/1‑93 (MQ=255)

                                              tggtggTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGc                                           >  2:5072454/1‑93 (MQ=255)

                                               ggtggtGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCt                                          >  2:3732539/1‑93 (MQ=255)

                                               ggtggtGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCt                                          >  1:1471775/1‑93 (MQ=255)

                                                gtggtgATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTGCATCGGCCCCTCGCtc                                         >  2:1123827/1‑92 (MQ=255)

                                                 tggtgATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGa                                        >  2:7588905/1‑93 (MQ=255)

                                                  ggtgATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGaa                                       <  1:912222/93‑1 (MQ=255)

                                                   gtgATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGaaa                                      <  1:3225982/93‑1 (MQ=255)

                                                     gATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAAtt                                    >  1:6489584/1‑93 (MQ=255)

                                                      aTCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGGCCACGTCCATCGGCTCTTCGCTGAAATTc                                   <  2:3117773/93‑1 (MQ=255)

                                                      aTCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTc                                   <  1:7175616/93‑1 (MQ=255)

                                                      aTCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTc                                   <  1:6405801/93‑1 (MQ=255)

                                                      aTCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTc                                   <  1:5291663/93‑1 (MQ=255)

                                                        taGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCTAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTg                                 <  1:6790897/92‑1 (MQ=255)

                                                          gCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGcc                               <  2:7770174/93‑1 (MQ=255)

                                                          gCCGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGcc                               >  1:1184394/1‑93 (MQ=255)

                                                            cGTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCAACCGGCTCTTCGCTGAAATcctgcctg                             >  1:7658661/1‑93 (MQ=255)

                                                            cGTGCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtg                             <  2:3487086/93‑1 (MQ=255)

                                                             gTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtgg                            <  1:3955066/93‑1 (MQ=255)

                                                             gTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtgg                            <  2:384970/93‑1 (MQ=255)

                                                             gTTCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtgg                            >  2:4645924/1‑93 (MQ=255)

                                                              ttCCCATGGGGATGCGGAACTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtggt                           <  2:4056113/93‑1 (MQ=255)

                                                              ttCCCATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtggg                           >  1:1718365/1‑92 (MQ=255)

                                                               taccATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtggtg                          >  2:4582228/3‑93 (MQ=255)

                                                                cccATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCtggtgg                         <  2:1008972/93‑1 (MQ=255)

                                                                 ccATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGc                        >  2:3156912/1‑93 (MQ=255)

                                                                 ccATGCGGATGCGGAGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGc                        >  1:5516052/1‑93 (MQ=255)

                                                                  tatgcggatgcggTGCTGAAAGTGCATTTGCTACAGCTTGGCGAACTTTTGACCATTTCCATCGGCTCTTCGCTGAAATTTTGCCTGTCGGCg                       >  1:6038340/2‑93 (MQ=255)

                                                                    tgcggatgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGa                     >  1:5779946/1‑93 (MQ=255)

                                                                     gcggatgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGaa                    >  2:1331946/1‑93 (MQ=255)

                                                                     gcggatgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGaa                    <  1:4285768/93‑1 (MQ=255)

                                                                        gatgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGa                 <  1:541092/93‑1 (MQ=255)

                                                                        gatgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGa                 <  2:620869/93‑1 (MQ=255)

                                                                         atgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGAc                <  2:336022/93‑1 (MQ=255)

                                                                         atgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGAc                <  1:7412038/93‑1 (MQ=255)

                                                                          tgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACa               <  1:2700974/93‑1 (MQ=255)

                                                                          tgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACa               <  1:4729350/93‑1 (MQ=255)

                                                                          tgcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAAGTCTGCCTGGTGGCGGAAGGACa               <  1:4218890/93‑1 (MQ=255)

                                                                           gcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAg              >  1:36901/1‑93 (MQ=255)

                                                                           gcggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAg              <  1:3831263/93‑1 (MQ=255)

                                                                            cggaGCTGAAATAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAgg             <  2:6969714/93‑1 (MQ=255)

                                                                            cggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAgg             >  1:4989395/1‑93 (MQ=255)

                                                                             ggaGCTGAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGgc            <  2:1695238/93‑1 (MQ=255)

                                                                                   gAAAGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGcg      >  2:4271567/1‑92 (MQ=255)

                                                                                    aaaGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTg     <  1:4471191/93‑1 (MQ=255)

                                                                                     aaGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGt    >  2:4393041/1‑93 (MQ=255)

                                                                                     aaGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGt    >  1:2525968/1‑93 (MQ=255)

                                                                                     aaGAGCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGt    >  1:1943684/1‑93 (MQ=255)

                                                                                      agagCATCTGNAACAGCTTGGNGNACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTa   <  1:7051111/93‑1 (MQ=255)

                                                                                      agagCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTa   >  2:5255383/1‑93 (MQ=255)

                                                                                       gagCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTTGCGGAAGGACAGGCGCAGCTGTAc  >  2:5719787/1‑93 (MQ=255)

                                                                                       gagCATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTAc  >  1:5584091/1‑93 (MQ=255)

TGCGGTGTGCGCAAGCAGATTCAGGTCCGCGATGCGCGCCCGCCGCTGGTGGTGATCAGCCGTTCCCATGCGGATGCGGAGCTGAAAGAGTATCTGCAACAGCTTGGCGAACATCAGACCACGTCCATCGGCTCTTCGCTGAAATTCTGCCTGGTGGCGGAAGGACAGGCGCAGCTGTAC  >  NC_000913/4434738‑4434917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`