BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,651	A→G	L427L (TTA→TTG)	ybbX →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,651	0	A	G	99.7%	1000.0 / ‑6.0	315	L427L (TTA→TTG)	ybbX	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (150/164); total (150/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 325 total aligned reads displayed.

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

tGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCg                                                                                         >  1:7577606/1‑93 (MQ=255)

  ttCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtg                                                                                       >  1:6919330/1‑93 (MQ=255)

    cTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggt                                                                                     <  1:4157695/93‑1 (MQ=255)

     ttACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggtg                                                                                    >  1:4694706/1‑93 (MQ=255)

     ttACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggtg                                                                                    >  2:6120679/1‑93 (MQ=255)

       aCCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgat                                                                                  <  1:6209169/93‑1 (MQ=255)

        ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                 >  1:6611629/1‑93 (MQ=255)

          aaTGACGATCTGGAATATCGCGACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                               >  2:5014421/1‑93 (MQ=255)

          aaTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtg                                                                               >  1:1919703/1‑93 (MQ=255)

           aTGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtga                                                                              <  2:3584068/93‑1 (MQ=255)

            tGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                             <  2:4683584/93‑1 (MQ=255)

             gACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTCCCATTGGCCCGCGTATCACGAAACCCATCTTGCGTGGTGATGTGAtt                                                                            <  1:6640202/93‑1 (MQ=255)

             gACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAtt                                                                            <  2:729452/93‑1 (MQ=255)

             gACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAtt                                                                            <  1:2765293/93‑1 (MQ=255)

              aCGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAttt                                                                           <  1:7865026/93‑1 (MQ=255)

              aCGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAttt                                                                           <  1:5040766/93‑1 (MQ=255)

               cGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTa                                                                          >  1:367944/1‑93 (MQ=255)

                gATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTAc                                                                         <  1:6737909/93‑1 (MQ=255)

                 aTCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGAGATGTGATTTACg                                                                        >  2:2307894/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  2:6919024/1‑93 (MQ=255)

                    tGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACa                                                                     <  2:2614338/93‑1 (MQ=255)

                    tGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACa                                                                     >  2:6416927/1‑93 (MQ=255)

                    tGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACa                                                                     >  1:5200230/1‑93 (MQ=255)

                    tGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACa                                                                     >  1:1305835/1‑93 (MQ=255)

                     ggAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                    <  2:6483050/93‑1 (MQ=255)

                     ggAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                    >  2:7793653/1‑93 (MQ=255)

                      gAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAtt                                                                   <  1:1076242/93‑1 (MQ=255)

                       aaTATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTg                                                                  >  1:442854/1‑93 (MQ=255)

                       aaTATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTg                                                                  >  1:6542761/1‑93 (MQ=255)

                        atatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGa                                                                 >  2:5516749/1‑93 (MQ=255)

                         tatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGaa                                                                >  1:1652463/1‑93 (MQ=255)

                         tatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGaa                                                                >  1:6348749/1‑93 (MQ=255)

                          atCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAc                                                               <  2:1474567/93‑1 (MQ=255)

                          atCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAc                                                               <  1:2291680/93‑1 (MQ=255)

                           tCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACa                                                              >  2:6200393/1‑93 (MQ=255)

                            cGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAg                                                             <  2:3935877/93‑1 (MQ=255)

                             gCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAGTTACGACATTGAACAgg                                                            >  1:7012142/1‑93 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          <  2:1067191/93‑1 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          <  2:5873436/93‑1 (MQ=255)

                                  aaaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTc                                                       >  1:548356/1‑93 (MQ=255)

                                   aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                      >  1:6147396/1‑93 (MQ=255)

                                     gTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCt                                                    <  1:1759156/93‑1 (MQ=255)

                                      tCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTg                                                   >  2:7333253/1‑93 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                >  2:5812773/1‑93 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                <  1:1374748/93‑1 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                <  1:7422402/93‑1 (MQ=255)

                                            cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                             >  2:2764561/1‑93 (MQ=255)

                                            cGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcgc                                             >  2:7501946/1‑93 (MQ=255)

                                               aTGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGa                                          <  2:7726480/93‑1 (MQ=255)

                                                 gTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaaa                                        >  2:4033718/1‑93 (MQ=255)

                                                 gTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaaa                                        <  2:1273243/93‑1 (MQ=255)

                                                    ggCCGTACCATTGGTGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGt                                     >  1:5736865/1‑93 (MQ=255)

                                                     gCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTc                                    <  1:3486793/93‑1 (MQ=255)

                                                       cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  >  2:3007479/1‑93 (MQ=255)

                                                       cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  <  1:4287529/93‑1 (MQ=255)

                                                       cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  <  1:2985549/93‑1 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 <  1:4606845/93‑1 (MQ=255)

                                                         tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAtt                                <  1:901497/93‑1 (MQ=255)

                                                         tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAtt                                <  2:2008395/93‑1 (MQ=255)

                                                         tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAtt                                >  1:6004427/1‑93 (MQ=255)

                                                           ccATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTa                              <  1:3758729/93‑1 (MQ=255)

                                                            cATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTAt                             >  2:1890271/1‑93 (MQ=255)

                                                            cATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTAt                             >  2:320030/1‑93 (MQ=255)

                                                            cATTGGCGCGCGGATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTAt                             <  2:5465606/93‑1 (MQ=255)

                                                               tGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCt                          <  2:2477964/93‑1 (MQ=255)

                                                                 gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                        >  1:7316876/1‑93 (MQ=255)

                                                                 gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                        >  2:5177271/1‑93 (MQ=255)

                                                                 gcgcgcGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTa                        >  2:512704/1‑93 (MQ=255)

                                                                  cgcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaa                       <  2:803826/93‑1 (MQ=255)

                                                                   gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                      <  1:1428079/93‑1 (MQ=255)

                                                                     gcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACa                    <  2:4108014/93‑1 (MQ=255)

                                                                      cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                   >  2:7185343/1‑93 (MQ=255)

                                                                      cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                   >  2:3435260/1‑93 (MQ=255)

                                                                      cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                   <  2:6611628/93‑1 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  >  1:401019/1‑93 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  <  2:1342395/93‑1 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  <  1:2846790/93‑1 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  >  1:3524389/1‑93 (MQ=255)

                                                                        tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                 <  1:1995206/93‑1 (MQ=255)

                                                                         gtCACGAAAACCATCTTGCGTGGGGATGTGATTTACGACATGGAACAGGGCTTCCCTGTGGCGCCGAAAGGTCAATTTATCCTTAAACATcag                <  1:2348657/92‑1 (MQ=255)

                                                                           cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca              <  2:3257268/93‑1 (MQ=255)

                                                                              gAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa           >  1:4237570/1‑93 (MQ=255)

                                                                              caaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa           <  2:4455308/92‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          <  2:5577986/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          >  2:7098708/1‑93 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          <  2:3117860/93‑1 (MQ=255)

                                                                               aaaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTaa          >  1:2689651/1‑93 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         <  2:1778512/93‑1 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         >  1:5238653/1‑93 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         >  1:1616072/1‑93 (MQ=255)

                                                                                aaaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAAt         >  1:1837067/1‑93 (MQ=255)

                                                                                 aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc        >  1:3867722/1‑93 (MQ=255)

                                                                                  aCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATcc       <  1:102631/93‑2 (MQ=255)

                                                                                  aCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCt       <  2:3802700/93‑1 (MQ=255)

                                                                                  aCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCt       >  2:3477653/1‑93 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg      >  1:2148433/1‑93 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg      <  1:7182877/93‑1 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   >  1:2461662/1‑93 (MQ=255)

                                                                                       cTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGccc  <  2:5722244/93‑1 (MQ=255)

                                                                                       cTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCCGGccc  <  1:2589971/93‑1 (MQ=255)

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`