Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,483,536 T→C intergenic (‑17/‑250) pepA ← / → yjgP aminopeptidase A/I/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,483,5360TC99.5% 1260.0 / ‑4.4 394intergenic (‑17/‑250)pepA/yjgPaminopeptidase A/I/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (195/196);  total (195/199)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 398 total aligned reads displayed.

GTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACG  >  NC_000913/4483444‑4483625
                                                                                            |                                                                                         
        aaGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGa                                                                                   <  1:5914003/93‑1 (MQ=255)
          gACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACa                                                                                 >  2:5943929/1‑93 (MQ=255)
           aCGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACaa                                                                                <  2:4185425/93‑1 (MQ=255)
           aCGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACaa                                                                                <  2:1326117/93‑1 (MQ=255)
            cGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAc                                                                               <  2:6635264/93‑1 (MQ=255)
            cGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAc                                                                               <  1:1644312/93‑1 (MQ=255)
            cGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAc                                                                               >  2:1918107/1‑93 (MQ=255)
               ccACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggc                                                                            <  1:4107946/93‑1 (MQ=255)
                cACGACGATTCAGGCACTCCGCTGTTCCTCCGGGCTACCGCTTTTTACACTAACCTCCATGCACTACGCTCCTGAACCTTAAAGACACcggcg                                                                           <  1:5725383/93‑1 (MQ=255)
                cACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcg                                                                           >  2:7648705/1‑93 (MQ=255)
                cACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcg                                                                           <  1:3170341/93‑1 (MQ=255)
                cACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcg                                                                           >  1:52896/1‑93 (MQ=255)
                 acgacgATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAAcggcgg                                                                          <  1:7851338/93‑1 (MQ=255)
                  cgacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGt                                                                         <  1:2181359/93‑1 (MQ=255)
                  cgacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGt                                                                         <  2:2751452/93‑1 (MQ=255)
                   gacgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTg                                                                        <  1:6170956/93‑1 (MQ=255)
                    acgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTgg                                                                       >  1:7343783/1‑93 (MQ=255)
                    acgaTGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTgg                                                                       >  2:2725282/1‑93 (MQ=255)
                        tGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTAc                                                                   >  1:7270408/1‑93 (MQ=255)
                         gCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACa                                                                  <  2:6849689/93‑1 (MQ=255)
                         gCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACa                                                                  <  1:3668874/93‑1 (MQ=255)
                           aGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGa                                                                >  2:1542871/1‑93 (MQ=255)
                           aGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGa                                                                >  1:1011190/1‑93 (MQ=255)
                            ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               >  1:676299/1‑93 (MQ=255)
                            ggCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGAt                                                               <  2:291449/93‑1 (MQ=255)
                               aCTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGa                                                            >  2:4809533/1‑93 (MQ=255)
                               aCTCCGCTGTTTCTCCGGGCTACCGCTTGTTAGACTAACCTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGa                                                            <  1:4012677/93‑1 (MQ=255)
                                cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           >  2:1152009/1‑93 (MQ=255)
                                cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           >  2:1594417/1‑93 (MQ=255)
                                cTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGaa                                                           >  2:404139/1‑93 (MQ=255)
                                 tCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAAt                                                          >  2:5101958/1‑93 (MQ=255)
                                 tCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAAt                                                          <  1:2083409/93‑1 (MQ=255)
                                    gCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGc                                                       >  1:5384421/1‑93 (MQ=255)
                                    gCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCCTAAAGACAACGGCGGTGGCTACAGATAGAATTGc                                                       <  1:5137967/93‑1 (MQ=255)
                                      tGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCaa                                                     <  1:1364624/93‑1 (MQ=255)
                                       gTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAg                                                    >  1:3062944/1‑93 (MQ=255)
                                       gTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAg                                                    <  2:1735335/93‑1 (MQ=255)
                                        tttCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGc                                                   <  2:3158021/93‑1 (MQ=255)
                                        tttCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGc                                                   <  1:6898609/93‑1 (MQ=255)
                                         ttCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCt                                                  >  1:2351123/1‑93 (MQ=255)
                                         ttCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCt                                                  <  1:880517/93‑1 (MQ=255)
                                          tctcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCtt                                                 >  1:1463950/1‑93 (MQ=255)
                                            tcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCtttt                                               <  2:6517439/93‑2 (MQ=255)
                                            tcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTc                                               <  2:6756702/93‑1 (MQ=255)
                                            tcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTc                                               >  1:1995267/1‑93 (MQ=255)
                                            tcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTc                                               >  2:2125848/1‑93 (MQ=255)
                                            tcCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTc                                               >  1:4573259/1‑93 (MQ=255)
                                             ccGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCg                                              <  1:7368849/93‑1 (MQ=255)
                                               gggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTa                                            >  1:6582798/1‑93 (MQ=255)
                                               gggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTa                                            <  2:3376800/93‑1 (MQ=255)
                                               gggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTa                                            >  2:5608588/1‑93 (MQ=255)
                                                ggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTaa                                           >  2:582278/1‑93 (MQ=255)
                                                ggCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTaa                                           >  1:4899104/1‑93 (MQ=255)
                                                  cTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACt                                         >  1:485748/1‑93 (MQ=255)
                                                   tACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTc                                        >  1:7618112/1‑93 (MQ=255)
                                                    aCCTCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCa                                       <  2:4413035/93‑1 (MQ=255)
                                                    aCCGCTTTTTACACTAAACTCCATGCCCTCCGCTCCTGAACCTTAAAGCCAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTACCTCa                                       <  1:375050/93‑1 (MQ=255)
                                                     ccGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCAt                                      <  2:5849014/93‑1 (MQ=255)
                                                     ccGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCAt                                      <  1:1770625/93‑1 (MQ=255)
                                                       gCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGt                                    >  2:7721195/1‑93 (MQ=255)
                                                       gCTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGt                                    >  2:7106517/1‑93 (MQ=255)
                                                        cTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTc                                   <  1:5160336/93‑1 (MQ=255)
                                                        cTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTc                                   <  2:7825675/93‑1 (MQ=255)
                                                        cTTTTTACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTc                                   <  1:5429507/93‑1 (MQ=255)
                                                         tttttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTcc                                  >  2:6246576/1‑93 (MQ=255)
                                                         tttttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTcc                                  >  1:3515099/1‑93 (MQ=255)
                                                           tttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGc                                <  2:4648262/93‑1 (MQ=255)
                                                           tttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGc                                <  2:254339/93‑1 (MQ=255)
                                                            ttACACTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCt                               <  2:4352441/93‑1 (MQ=255)
                                                              acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             <  2:5329084/93‑1 (MQ=255)
                                                              acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             <  1:4514561/93‑1 (MQ=255)
                                                              acacTAAACTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGt                             >  1:620135/1‑93 (MQ=255)
                                                                    aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       <  1:3374133/93‑1 (MQ=255)
                                                                    aaCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGAt                       >  1:1852742/1‑93 (MQ=255)
                                                                     aCTCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATg                      >  1:5599040/1‑93 (MQ=255)
                                                                       tCCATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGAc                    <  2:5205789/93‑1 (MQ=255)
                                                                         cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  <  1:1183760/93‑1 (MQ=255)
                                                                         cATGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACtt                  <  1:7171999/93‑1 (MQ=255)
                                                                          aTGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTc                 <  2:3835634/93‑1 (MQ=255)
                                                                          aTGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTc                 <  2:6451554/93‑1 (MQ=255)
                                                                          aTGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTAGCTACAGATAGAATTCCAAGCTTTTGTAACTCATGTCCGCTGTTCCGATGACTTc                 <  2:2374723/93‑1 (MQ=255)
                                                                           tGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCg                >  2:3513057/1‑93 (MQ=255)
                                                                           tGCACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCg                >  1:2604462/1‑93 (MQ=255)
                                                                             cACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtg              <  2:6084299/93‑1 (MQ=255)
                                                                             cACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtg              >  1:6116721/1‑93 (MQ=255)
                                                                             cACTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtg              <  2:3756039/93‑1 (MQ=255)
                                                                              aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGTATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             >  2:7019697/1‑93 (MQ=255)
                                                                              aCTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCgtgt             >  1:2918081/1‑93 (MQ=255)
                                                                               cTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGtt            <  1:5317821/93‑1 (MQ=255)
                                                                               cTACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGtt            <  2:7444470/93‑1 (MQ=255)
                                                                                tACGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTa           >  1:4350369/1‑93 (MQ=255)
                                                                                 aCGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTaa          >  1:2472186/1‑93 (MQ=255)
                                                                                 aCGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTaa          >  2:723781/1‑93 (MQ=255)
                                                                                  cGCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAAt         <  1:7652109/93‑1 (MQ=255)
                                                                                   gCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATc        >  1:2259830/1‑93 (MQ=255)
                                                                                   gCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATc        >  1:2757543/1‑93 (MQ=255)
                                                                                   gCTCCTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATc        <  1:3791635/93‑1 (MQ=255)
                                                                                       cTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTaa    <  1:7423710/93‑1 (MQ=255)
                                                                                       cTGAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTaa    <  2:4968012/93‑1 (MQ=255)
                                                                                         gAACCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACg  >  1:7584099/1‑93 (MQ=255)
                                                                                            |                                                                                         
GTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACTACGCTCCTGAATCTTAAAGACAACGGCGGTGGCTACAGATAGAATTGCAAGCTTTCGTAACTCATGTCCGCTGTTGCGATGACTTCGTGTTAATCTTAACG  >  NC_000913/4483444‑4483625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑