BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,484,843	T→C	V353A (GTC→GCC)	yjgP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,484,843	0	T	C	99.4%	1090.2 / ‑4.4	355	V353A (GTC→GCC)	yjgP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (185/168); total (186/169)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 370 total aligned reads displayed.

GTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGTCCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTT  >  NC_000913/4484753‑4484932

 tAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccg                                                                                        >  2:7341478/1‑93 (MQ=255)

  aaGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccgc                                                                                       <  2:1348222/93‑1 (MQ=255)

  aaGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccgc                                                                                       >  1:2522090/1‑93 (MQ=255)

   aGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccgcc                                                                                      >  2:7916269/1‑93 (MQ=255)

    gCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCccgcca                                                                                     >  1:1671484/1‑92 (MQ=255)

       ggACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCCCCt                                                                                  >  2:5791407/1‑93 (MQ=255)

        gACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTg                                                                                 >  1:4017041/1‑93 (MQ=255)

         aCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTgc                                                                                <  2:3501677/93‑1 (MQ=255)

           ccGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCCACCTTTGGGACACCGTGCCGGCCCGCCCCCTgccc                                                                              >  2:1459513/1‑91 (MQ=255)

                 cTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGt                                                                        <  2:632085/93‑1 (MQ=255)

                  tgtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGtt                                                                       >  1:2267506/1‑93 (MQ=255)

                  tgtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGtt                                                                       >  1:4776942/1‑93 (MQ=255)

                   gtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttt                                                                      <  2:2924801/93‑1 (MQ=255)

                    tggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGtttt                                                                     <  1:3279788/93‑1 (MQ=255)

                     ggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttttt                                                                    <  1:1501470/93‑1 (MQ=255)

                      gatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTc                                                                   >  1:2145594/1‑93 (MQ=255)

                      gatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTc                                                                   <  2:7135437/93‑1 (MQ=255)

                       atgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTcg                                                                  >  2:4382668/1‑93 (MQ=255)

                        tgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTcgc                                                                 >  2:4745109/1‑93 (MQ=255)

                        tgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTcgc                                                                 >  1:112044/1‑93 (MQ=255)

                          tggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGt                                                               <  1:4131293/93‑1 (MQ=255)

                           ggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTa                                                              >  1:2012848/1‑93 (MQ=255)

                           ggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTa                                                              >  1:5549070/1‑93 (MQ=255)

                              ccGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAg                                                           <  2:1148244/93‑1 (MQ=255)

                              ccGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAg                                                           <  2:1886789/93‑1 (MQ=255)

                              ccGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAg                                                           <  1:4230840/93‑1 (MQ=255)

                               cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTTGGACACCGTGCCGGCCCGCCTCCTGCGCGCCAGTTTTTCGCGTAAAgg                                                          <  1:1400665/93‑1 (MQ=255)

                               cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAgg                                                          >  1:6122815/1‑93 (MQ=255)

                               cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAgg                                                          >  1:4482827/1‑93 (MQ=255)

                                gTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                         >  1:3623273/1‑93 (MQ=255)

                                gTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                         >  1:2410642/1‑93 (MQ=255)

                                  tAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGc                                                       <  1:5783785/93‑1 (MQ=255)

                                  tAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGc                                                       <  1:4932262/93‑1 (MQ=255)

                                  tAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGc                                                       <  2:1607437/93‑1 (MQ=255)

                                    aCCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCgg                                                     <  2:5014991/93‑1 (MQ=255)

                                     ccTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgt                                                    >  2:7270521/1‑93 (MQ=255)

                                      cTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtg                                                   <  1:6582469/93‑1 (MQ=255)

                                       tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgt                                                  <  2:2571287/93‑1 (MQ=255)

                                       tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgt                                                  <  2:1069033/93‑1 (MQ=255)

                                       tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgg                                                  >  2:5632355/1‑92 (MQ=255)

                                       tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgg                                                  >  2:2211456/1‑92 (MQ=255)

                                       tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGAACCAGTTTTTCGCGTAAAGGAGCGgtgg                                                  >  1:1005014/1‑92 (MQ=255)

                                        gATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGtgtg                                                 <  2:6619503/93‑1 (MQ=255)

                                         aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                >  1:3868026/1‑93 (MQ=255)

                                         aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                <  1:5202758/93‑1 (MQ=255)

                                         aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                <  1:6273176/93‑1 (MQ=255)

                                          tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                               <  1:7575085/93‑1 (MQ=255)

                                          tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                               >  1:1815680/1‑93 (MQ=255)

                                          tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                               >  2:6741115/1‑93 (MQ=255)

                                          tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                               <  1:3123870/93‑1 (MQ=255)

                                           ttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATg                                              >  2:4897709/1‑93 (MQ=255)

                                            tATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGc                                             >  1:3365233/1‑93 (MQ=255)

                                            tATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGc                                             >  1:7786630/1‑93 (MQ=255)

                                              tCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCaa                                           >  2:5845678/1‑93 (MQ=255)

                                              tCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGGTTTTTGCGTAAAGGAGCGGTGTGATGCaa                                           >  2:2965869/1‑93 (MQ=255)

                                               cTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAc                                          <  1:5477184/93‑1 (MQ=255)

                                                tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                         >  1:6658441/1‑93 (MQ=255)

                                                tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                         <  1:6471748/93‑1 (MQ=255)

                                                 ggCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCt                                        <  1:1191513/93‑1 (MQ=255)

                                                  gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                       >  2:5361481/1‑93 (MQ=255)

                                                  gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTCCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                       >  2:2141880/1‑93 (MQ=255)

                                                   cTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCttt                                      >  2:2311417/1‑93 (MQ=255)

                                                    tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                     <  1:6790160/93‑1 (MQ=255)

                                                       aGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGc                                  <  1:5889838/93‑1 (MQ=255)

                                                       aGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGc                                  <  2:4030825/93‑1 (MQ=255)

                                                          gATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTa                               <  1:7377007/93‑1 (MQ=255)

                                                           aTTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTAc                              >  1:7490364/1‑93 (MQ=255)

                                                             tGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACtt                            <  2:5139607/93‑1 (MQ=255)

                                                             tGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACtt                            <  1:2822004/93‑1 (MQ=255)

                                                              gTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTg                           >  1:7108466/1‑93 (MQ=255)

                                                               ttCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGa                          >  2:6466952/1‑93 (MQ=255)

                                                                 ctcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAcc                        >  2:4249384/1‑93 (MQ=255)

                                                                  tcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTCTTGGCGTACTTGAcag                       >  2:2693596/1‑91 (MQ=255)

                                                                    aaCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCt                     >  1:2713019/1‑93 (MQ=255)

                                                                    aaCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCt                     >  2:5863676/1‑93 (MQ=255)

                                                                      ccTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                   <  1:2974105/93‑1 (MQ=255)

                                                                      ccTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                   <  2:1281092/93‑1 (MQ=255)

                                                                       cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGGTGCAACCTTTTGGCGTACTTGACCGCtata                  >  2:5510314/1‑93 (MQ=255)

                                                                       cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                  <  2:3404587/93‑1 (MQ=255)

                                                                       cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                  <  2:6809624/93‑1 (MQ=255)

                                                                        tttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTTGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTatat                 >  2:3856708/1‑93 (MQ=255)

                                                                         ttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATc                <  2:701999/93‑1 (MQ=255)

                                                                         ttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATc                <  1:5283403/93‑1 (MQ=255)

                                                                         ttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATc                >  1:1649903/1‑93 (MQ=255)

                                                                          tGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCg               <  2:5095594/93‑1 (MQ=255)

                                                                          tGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCg               <  2:3655838/93‑1 (MQ=255)

                                                                           gggACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCgg              <  2:1809377/93‑1 (MQ=255)

                                                                           gggACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCgg              >  2:4003174/1‑93 (MQ=255)

                                                                            ggACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCgga             >  1:1238043/1‑92 (MQ=255)

                                                                               cacCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaa          >  2:3153952/1‑93 (MQ=255)

                                                                                acCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaaa         >  1:5836329/1‑93 (MQ=255)

                                                                                 ccGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAc        >  2:6121187/1‑93 (MQ=255)

                                                                                 ccGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAc        >  2:7431160/1‑93 (MQ=255)

                                                                                  cGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACt       <  2:3562011/93‑1 (MQ=255)

                                                                                  cGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACt       >  2:4498130/1‑93 (MQ=255)

                                                                                   gTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTa      <  1:999205/93‑1 (MQ=255)

                                                                                    tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt     >  1:4358175/1‑93 (MQ=255)

                                                                                      ccGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAttt   <  1:6970424/93‑1 (MQ=255)

                                                                                      ccGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAttt   <  2:4317410/93‑1 (MQ=255)

                                                                                      ccGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAttt   <  1:78990/93‑1 (MQ=255)

GTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGTCCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTT  >  NC_000913/4484753‑4484932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`