BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,484,849	G→A	R355H (CGC→CAC)	yjgP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,484,849	0	G	A	99.4%	1138.7 / ‑4.6	335	R355H (CGC→CAC)	yjgP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (171/159); total (173/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 375 total aligned reads displayed.

TGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGTCCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCAC  >  NC_000913/4484759‑4484938

tGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCgccacc                                                                                         <  1:5807229/93‑1 (MQ=255)

tGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCgccacc                                                                                         >  2:5100875/1‑93 (MQ=255)

   aCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTgc                                                                                      >  2:1901637/1‑93 (MQ=255)

       gACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTgcgcgc                                                                                  >  2:7324351/1‑93 (MQ=255)

       gACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTgcgcgc                                                                                  >  2:694254/1‑93 (MQ=255)

         cGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCa                                                                                <  2:4885080/93‑1 (MQ=255)

          gCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAg                                                                               <  2:6424726/93‑1 (MQ=255)

            tgtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGTCAggt                                                                             >  2:1304061/1‑91 (MQ=255)

             gtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttt                                                                            >  1:4812951/1‑93 (MQ=255)

             gtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttt                                                                            >  1:6116638/1‑93 (MQ=255)

             gtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttt                                                                            <  2:2924802/93‑1 (MQ=255)

             gtggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttt                                                                            >  2:5060262/1‑93 (MQ=255)

              tggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGtttt                                                                           <  1:3279788/93‑1 (MQ=255)

              tggatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGtttt                                                                           >  1:1175695/1‑93 (MQ=255)

               ggatgtggaCCGTTAACCCTATTTATCTGCCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGttttt                                                                          <  2:5834107/93‑1 (MQ=255)

                gatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTc                                                                         <  1:3895488/93‑1 (MQ=255)

                gatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTc                                                                         <  1:4248047/93‑1 (MQ=255)

                gatgtggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTc                                                                         <  2:4465410/93‑1 (MQ=255)

                    tggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGt                                                                     <  1:4131293/93‑1 (MQ=255)

                     ggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTa                                                                    >  1:2012848/1‑93 (MQ=255)

                     ggaCCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTa                                                                    >  1:5549070/1‑93 (MQ=255)

                        ccGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAg                                                                 >  2:4620316/1‑93 (MQ=255)

                        ccGTTAACCTGATTTATCTGGCTCTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAg                                                                 <  2:4298177/93‑1 (MQ=255)

                         cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTTGGACACCGTGCCGGCCCGCCTCCTGCGCGCCAGTTTTTCGCGTAAAgg                                                                <  1:1400665/93‑1 (MQ=255)

                         cGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAgg                                                                >  1:4482827/1‑93 (MQ=255)

                          gTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                               >  2:3074933/1‑93 (MQ=255)

                          gTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGa                                                               >  1:3497978/1‑93 (MQ=255)

                               ccTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgt                                                          <  1:107185/93‑1 (MQ=255)

                               ccTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgt                                                          <  2:6707541/93‑1 (MQ=255)

                                cTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtg                                                         <  1:6582469/93‑1 (MQ=255)

                                 tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgt                                                        <  2:2571287/93‑1 (MQ=255)

                                 tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgt                                                        >  2:3758294/1‑93 (MQ=255)

                                 tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgt                                                        <  2:1069033/93‑1 (MQ=255)

                                 tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgg                                                        >  2:2211456/1‑92 (MQ=255)

                                 tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGgtgg                                                        >  2:1431810/1‑92 (MQ=255)

                                 tGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGAACCAGTTTTTCGCGTAAAGGAGCGgtgg                                                        >  1:1005014/1‑92 (MQ=255)

                                   aTTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGa                                                      <  1:5202758/93‑1 (MQ=255)

                                    tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                                     >  1:1815680/1‑93 (MQ=255)

                                    tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                                     >  2:4973781/1‑93 (MQ=255)

                                    tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                                     <  1:3123870/93‑1 (MQ=255)

                                    tttATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGAt                                                     <  2:149182/93‑1 (MQ=255)

                                      tATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGc                                                   >  1:3365233/1‑93 (MQ=255)

                                       aTCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCa                                                  >  2:7911032/1‑93 (MQ=255)

                                        tCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCaa                                                 <  2:3569483/93‑1 (MQ=255)

                                         cTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAc                                                <  1:5477184/93‑1 (MQ=255)

                                          tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                               >  1:6658441/1‑93 (MQ=255)

                                          tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                               >  1:7826276/1‑93 (MQ=255)

                                          tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                               >  2:3539262/1‑93 (MQ=255)

                                          tGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAAcc                                               <  1:6471748/93‑1 (MQ=255)

                                           ggCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCt                                              <  1:2781385/93‑1 (MQ=255)

                                            gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                             >  2:5361481/1‑93 (MQ=255)

                                            gCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTCCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtt                                             >  2:2141880/1‑93 (MQ=255)

                                             cTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCttt                                            >  2:2311417/1‑93 (MQ=255)

                                              tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                           <  1:6790160/93‑1 (MQ=255)

                                              tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                           <  1:2632626/93‑1 (MQ=255)

                                              tttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCtttt                                           <  1:2384811/93‑1 (MQ=255)

                                               ttAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTg                                          >  1:503225/1‑93 (MQ=255)

                                                tAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTgg                                         >  1:1237594/1‑93 (MQ=255)

                                                 aGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGc                                        <  2:7066357/93‑1 (MQ=255)

                                                     aTTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTAc                                    >  2:5672309/1‑93 (MQ=255)

                                                     aTTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTAc                                    <  2:1215467/93‑1 (MQ=255)

                                                     aTTGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTAc                                    >  1:7495840/1‑93 (MQ=255)

                                                       tGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACtt                                  >  2:3966293/1‑93 (MQ=255)

                                                       tGTTCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACtt                                  <  2:6795336/93‑1 (MQ=255)

                                                         ttCTCAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTgc                                >  1:2199720/1‑92 (MQ=255)

                                                           ctcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAcc                              <  2:6170811/93‑1 (MQ=255)

                                                           ctcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGAcc                              >  1:382635/1‑93 (MQ=255)

                                                            tcAACCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCg                             >  1:6211943/1‑93 (MQ=255)

                                                              aaCCTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCt                           >  2:5863676/1‑93 (MQ=255)

                                                                ccTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtat                         <  2:7191283/93‑1 (MQ=255)

                                                                 cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGGTGCAACCTTTTGGCGTACTTGACCGCtata                        >  2:5510314/1‑93 (MQ=255)

                                                                 cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                        <  2:6101135/93‑1 (MQ=255)

                                                                 cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                        <  1:2075412/93‑1 (MQ=255)

                                                                 cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                        <  2:4375916/93‑1 (MQ=255)

                                                                 cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                        <  1:5679862/93‑1 (MQ=255)

                                                                 cTTTGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCtata                        >  1:1476822/1‑93 (MQ=255)

                                                                  tttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTatat                       <  1:5872258/93‑1 (MQ=255)

                                                                  tttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTatat                       >  1:7905694/1‑93 (MQ=255)

                                                                   ttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATc                      <  1:5283403/93‑1 (MQ=255)

                                                                   ttGGGACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATc                      >  1:1649903/1‑93 (MQ=255)

                                                                      ggACACCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGt                   <  2:182937/93‑1 (MQ=255)

                                                                        acacCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaa                 <  1:7432221/93‑1 (MQ=255)

                                                                         cacCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaa                >  1:245390/1‑93 (MQ=255)

                                                                          acCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaaa               <  2:4080210/93‑1 (MQ=255)

                                                                          acCGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTaaaa               >  1:7272602/1‑93 (MQ=255)

                                                                           ccGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAGAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAc              <  2:6593087/93‑1 (MQ=255)

                                                                           ccGTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAAc              >  2:7431160/1‑93 (MQ=255)

                                                                             gTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCATCCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTa            <  2:401376/93‑1 (MQ=255)

                                                                             gTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTa            >  1:4017604/1‑93 (MQ=255)

                                                                             gTGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTa            <  1:999205/93‑1 (MQ=255)

                                                                              tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt           >  2:2733867/1‑93 (MQ=255)

                                                                              tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAt           >  1:4358175/1‑93 (MQ=255)

                                                                              tGCCGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTCTTAGCGTACTTGACTGCTATATCGGTAAAACTAt           >  2:3272097/1‑93 (MQ=255)

                                                                                ccGGCCCGCCACCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTAttt         <  1:6970424/93‑1 (MQ=255)

                                                                                  ggcccgccaCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTc       <  2:5710925/90‑1 (MQ=255)

                                                                                  ggcccgccaCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTc       <  2:1718881/90‑1 (MQ=255)

                                                                                   gcccgccaCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTca      <  1:2914030/91‑1 (MQ=255)

                                                                                   gcccgccaCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTca      >  2:34779/3‑93 (MQ=255)

                                                                                   gcccgccaCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTca      >  2:2986594/3‑93 (MQ=255)

                                                                                       gccaccTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTcaccac  >  1:7606840/1‑93 (MQ=255)

TGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACCGTGCCGGTCCGCCGCCTGCGCGCCAGTTTTTCGCGTAAAGGAGCGGTGTGATGCAACCTTTTGGCGTACTTGACCGCTATATCGGTAAAACTATTTTCACCAC  >  NC_000913/4484759‑4484938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`