BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,511,632	T→C	Q84R (CAG→CGG)	fecB ←	FecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,511,632	0	T	C	99.7%	1114.1 / ‑5.4	362	Q84R (CAG→CGG)	fecB	FecB
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (174/186); total (174/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 399 total aligned reads displayed.

CCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCG  >  NC_000913/4511542‑4511721

cccGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGc                                                                                         >  2:7612377/1‑93 (MQ=255)

  cGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGccc                                                                                       >  2:7822737/1‑92 (MQ=255)

     aTGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGGTCCGACGGACCGCCACgg                                                                                    >  2:4958910/1‑93 (MQ=255)

      tGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGGCCGCCACGGt                                                                                   >  2:2889337/1‑93 (MQ=255)

      tGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGt                                                                                   >  1:506640/1‑93 (MQ=255)

      tGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGt                                                                                   >  1:844345/1‑93 (MQ=255)

      tGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGACCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGt                                                                                   <  1:7403978/93‑1 (MQ=255)

       ggCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGtt                                                                                  >  2:6498152/1‑93 (MQ=255)

          gagtgctgTCGTCAAGGAACAGGTCTGGTTTGAGAGCGGCAAAGGCTCCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCTACGGTTTCa                                                                               <  2:4395756/90‑1 (MQ=255)

            ctgctgTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAgg                                                                             >  1:6786741/1‑93 (MQ=255)

             tgctgTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAggg                                                                            >  2:1299897/1‑92 (MQ=255)

              gctgTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTg                                                                           <  1:7117284/93‑1 (MQ=255)

              gctgTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGGTTCAggcg                                                                           >  2:2802239/1‑91 (MQ=255)

                tgTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTgcg                                                                         >  2:2469081/1‑93 (MQ=255)

                tgTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTgcg                                                                         >  2:5816144/1‑93 (MQ=255)

                  tCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCCCGGTTTCCGGTgcgcc                                                                       >  2:2384144/1‑92 (MQ=255)

                  tCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGcgca                                                                       >  1:4285925/1‑93 (MQ=255)

                  tCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGcgca                                                                       <  1:6242069/93‑1 (MQ=255)

                   cGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGcgcac                                                                      <  1:3850123/93‑1 (MQ=255)

                      cAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGcgcacgca                                                                   <  1:592973/93‑1 (MQ=255)

                       aaTGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCgcacgcac                                                                  <  2:5367557/93‑1 (MQ=255)

                        aTGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACt                                                                 >  2:2521130/1‑93 (MQ=255)

                        aTGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACt                                                                 >  2:4181472/1‑93 (MQ=255)

                        aTGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACt                                                                 <  1:3045783/93‑1 (MQ=255)

                         tGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACtt                                                                >  1:779579/1‑93 (MQ=255)

                           aTCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGCTTCAGGGGAGCACGCACTTCg                                                              >  1:1839241/1‑93 (MQ=255)

                            tCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGGTGCGCGCGCGTGCCGACGGACCGCCACGGTTTCAGGGGCGCACCCCCTTCgg                                                             >  1:241563/1‑93 (MQ=255)

                            tCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCgg                                                             <  1:1721124/93‑1 (MQ=255)

                             cAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCggg                                                            <  2:7459259/93‑1 (MQ=255)

                              aGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGATCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGATTTATGTGCGCACGCACTTCGGGc                                                           >  2:824564/1‑93 (MQ=255)

                               ggTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCa                                                          <  2:3577987/93‑1 (MQ=255)

                               ggTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCa                                                          <  2:7527537/93‑1 (MQ=255)

                               ggTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCa                                                          >  2:6016996/1‑93 (MQ=255)

                               ggTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACGTCGGGCa                                                          >  2:3623196/1‑93 (MQ=255)

                                gTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAg                                                         >  2:1214506/1‑93 (MQ=255)

                                 tCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAgg                                                        >  1:3692335/1‑93 (MQ=255)

                                 tCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAgg                                                        >  1:7709537/1‑93 (MQ=255)

                                 tCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGGTTCAGGTGCGCACGCACTTCGGGCAgg                                                        >  2:5927917/1‑93 (MQ=255)

                                  cTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGa                                                       <  1:2902714/93‑1 (MQ=255)

                                  cTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGa                                                       <  2:2205127/93‑1 (MQ=255)

                                  cTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGa                                                       >  2:1973278/1‑93 (MQ=255)

                                   tGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGAt                                                      <  2:2606593/93‑1 (MQ=255)

                                      tttCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCg                                                   >  1:1998255/1‑93 (MQ=255)

                                       ttCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGt                                                  <  2:5217921/93‑1 (MQ=255)

                                        tCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGtt                                                 >  2:6588843/1‑93 (MQ=255)

                                        tCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGtt                                                 >  2:7657467/1‑93 (MQ=255)

                                         cAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGttt                                                <  2:6761640/93‑1 (MQ=255)

                                         cAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGttt                                                <  2:5534916/93‑1 (MQ=255)

                                          agagCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGtttt                                               <  1:105551/93‑1 (MQ=255)

                                          agagCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGtttt                                               <  1:6460322/93‑1 (MQ=255)

                                          agagCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGtttt                                               >  1:5563819/1‑93 (MQ=255)

                                          agagCCGCAATGACTTCCAGGCTCGGCGCCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGtttt                                               <  1:319435/93‑1 (MQ=255)

                                            agCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGc                                             >  2:5632566/1‑93 (MQ=255)

                                            agCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGc                                             >  1:376234/1‑93 (MQ=255)

                                             gCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCa                                            >  2:1519141/1‑93 (MQ=255)

                                             gCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCa                                            >  1:346785/1‑93 (MQ=255)

                                             gCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCa                                            <  2:4576081/93‑1 (MQ=255)

                                              cGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCAt                                           <  1:4602949/93‑1 (MQ=255)

                                               ggCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATc                                          <  1:1009961/93‑1 (MQ=255)

                                               ggCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATc                                          <  1:7551669/93‑1 (MQ=255)

                                                 cAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGt                                        <  1:1476262/93‑1 (MQ=255)

                                                 cAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGt                                        <  2:373092/93‑1 (MQ=255)

                                                  aaTGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGtt                                       <  1:1202466/93‑1 (MQ=255)

                                                  aaTGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGtt                                       <  1:4925534/93‑1 (MQ=255)

                                                   aTGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTa                                      <  2:7773128/93‑1 (MQ=255)

                                                    tGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAt                                     <  1:4796919/93‑1 (MQ=255)

                                                     ggCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAtc                                    >  2:7924443/1‑93 (MQ=255)

                                                       cTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAtcgt                                  <  2:7259929/93‑1 (MQ=255)

                                                        ttCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGGGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAtcgtg                                 >  1:6983276/1‑92 (MQ=255)

                                                         tCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTAtcgtcg                                >  2:1089742/1‑93 (MQ=255)

                                                          ccAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCCGGCAGGATGCGTTTTGCATCGTTATCGTCgg                               >  2:4722453/1‑93 (MQ=255)

                                                           cAGGCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGc                              >  2:3007922/1‑93 (MQ=255)

                                                             ggCTCGGCTGCGCGCGCGTTCCGACGGACCGCCCCGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCCTCGTTATCGTCCGCaa                            >  2:6207021/1‑93 (MQ=255)

                                                             ggCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCaa                            <  1:137044/93‑1 (MQ=255)

                                                             ggCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCaa                            <  2:6322562/93‑1 (MQ=255)

                                                             ggCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCaa                            <  1:4023561/93‑1 (MQ=255)

                                                             ggCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCaa                            <  2:1834051/93‑1 (MQ=255)

                                                             ggCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGGTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCaa                            >  1:5811164/1‑93 (MQ=255)

                                                              gCTCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAAt                           >  1:3316842/1‑93 (MQ=255)

                                                               gtCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATa                          <  1:6190678/92‑1 (MQ=255)

                                                                tCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATAc                         >  2:4321850/1‑93 (MQ=255)

                                                                tCGGCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATAc                         <  2:3036117/93‑1 (MQ=255)

                                                                  ggCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCg                       >  1:7199644/1‑93 (MQ=255)

                                                                   gCTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGGTTTGCATCGTTATCGTCGGCAATACCGa                      >  2:7090206/1‑93 (MQ=255)

                                                                    cTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGAt                     >  1:6042433/1‑93 (MQ=255)

                                                                    cTGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGAt                     >  1:3246573/1‑93 (MQ=255)

                                                                     tGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATc                    >  1:4426276/1‑93 (MQ=255)

                                                                     tGCGCGCGCGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATc                    <  1:2133789/93‑1 (MQ=255)

                                                                      gcgcgcgcGTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCg                   >  2:3957615/1‑93 (MQ=255)

                                                                       cgcgcgcgTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCgg                  >  2:1772752/1‑93 (MQ=255)

                                                                        gcgcgcgTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCggg                 <  2:683232/93‑1 (MQ=255)

                                                                         cgcgcgTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGa                <  1:5095245/93‑1 (MQ=255)

                                                                         cgcgcgTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGa                >  1:3953096/1‑93 (MQ=255)

                                                                          gcgcgTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGAt               >  2:3323354/1‑93 (MQ=255)

                                                                            gcgTTCCGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGa             >  1:1276627/1‑93 (MQ=255)

                                                                                 ccGACGGCCCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTcc        <  2:4467767/93‑1 (MQ=255)

                                                                                 ccGACGGACCTCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTcc        <  1:2641840/93‑1 (MQ=255)

                                                                                 ccGACGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTcc        <  2:6700643/93‑1 (MQ=255)

                                                                                    aCGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCAcg     >  2:4034417/1‑93 (MQ=255)

                                                                                    aCGGACCGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCAcg     <  1:3019239/93‑1 (MQ=255)

CCCGCATGGCGACTGCTGTCGGCAATGATCAGGTCTGGTTTCAGAGCGGCAATGGCTTCCAGGCTCGGCTGCGCGCGCGTTCCGACGGACTGCCACGGTTTCAGGTGCGCACGCACTTCGGGCAGGATGCGTTTTGCATCGTTATCGTCGGCAATACCGATCGGGATGACGTCCACGGCG  >  NC_000913/4511542‑4511721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`