BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,662	A→G	V195A (GTC→GCC)	fecA ←	FecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,662	0	A	G	99.7%	887.1 / ‑6.1	285	V195A (GTC→GCC)	fecA	FecA
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (147/137); total (147/138)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 306 total aligned reads displayed.

ATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGC  >  NC_000913/4513572‑4513751

 tGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCgg                                                                                        >  2:1242543/1‑93 (MQ=255)

   gCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTc                                                                                      <  1:5418703/93‑1 (MQ=255)

   gCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTc                                                                                      >  2:4894726/1‑93 (MQ=255)

     aTCGATGTTCCCGAGCGTAACGGGAGCGAGTTAAAGCTGCGGCTGACCGTAAGGGCCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGa                                                                                    <  1:3917809/93‑1 (MQ=255)

     aTCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGa                                                                                    >  1:4454628/1‑93 (MQ=255)

     aTCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGa                                                                                    <  2:7401619/93‑1 (MQ=255)

     aTCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGGCGa                                                                                    >  2:2980271/1‑93 (MQ=255)

      tCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAg                                                                                   >  2:3583838/1‑93 (MQ=255)

       ccATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCgggg                                                                                  >  2:1939993/1‑90 (MQ=255)

            ttGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTg                                                                             >  2:1629091/1‑93 (MQ=255)

            ttGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTg                                                                             <  1:116250/93‑1 (MQ=255)

              gCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGAGGTCGAGCGGCTGGc                                                                           >  1:1000213/1‑93 (MQ=255)

                cGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGa                                                                         <  2:348713/93‑1 (MQ=255)

                cGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGa                                                                         <  1:2296833/93‑1 (MQ=255)

                 gAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCATGGCGGTCGAGCGGCTGGCGAg                                                                        <  2:7771107/93‑1 (MQ=255)

                   gCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTGCATCAGGGCGGTCGAGCGGCTGGCGAGgc                                                                      >  1:5687145/1‑93 (MQ=255)

                   gCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGgc                                                                      <  1:1661336/93‑1 (MQ=255)

                   gCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGgc                                                                      <  1:7555557/93‑1 (MQ=255)

                     gAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGgcgg                                                                    >  2:1413199/1‑92 (MQ=255)

                      aaaCGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGcgcg                                                                   >  2:7848830/1‑93 (MQ=255)

                      aaaCGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGGCCATCAGGGCGGTCGAGCGGCTGGCGAGgcgag                                                                   >  1:4895914/1‑91 (MQ=255)

                        aCGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCggg                                                                 <  1:122089/93‑1 (MQ=255)

                        aCGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCggg                                                                 <  1:2559544/93‑1 (MQ=255)

                        aCGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCggg                                                                 >  1:5304780/1‑93 (MQ=255)

                          gggAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCggggt                                                               >  1:1483304/1‑91 (MQ=255)

                           ggAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTc                                                              <  1:7745187/93‑1 (MQ=255)

                           ggAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTc                                                              <  1:4731365/93‑1 (MQ=255)

                           ggAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTc                                                              >  2:6356114/1‑93 (MQ=255)

                            gAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCa                                                             <  2:11140/93‑1 (MQ=255)

                            gAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCa                                                             <  1:6499202/93‑1 (MQ=255)

                            gAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTCa                                                             >  1:3401994/1‑93 (MQ=255)

                            gAGCAAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCTGGTTCa                                                             <  2:2373756/93‑1 (MQ=255)

                             aGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTCAg                                                            >  2:173560/1‑93 (MQ=255)

                               ccAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGc                                                          <  2:1373934/93‑1 (MQ=255)

                                cAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTCAGGcc                                                         >  1:86540/1‑93 (MQ=255)

                                 aGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGccc                                                        <  1:6221343/93‑1 (MQ=255)

                                  gTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGcccc                                                       >  2:5562027/1‑93 (MQ=255)

                                   tGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGGCCCg                                                      >  1:3575916/1‑93 (MQ=255)

                                   tGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCg                                                      >  1:2001472/1‑93 (MQ=255)

                                    gAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCgg                                                     >  1:3286225/1‑93 (MQ=255)

                                    gAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCgg                                                     <  1:6148671/93‑1 (MQ=255)

                                     aaaGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGa                                                    <  2:5591644/93‑1 (MQ=255)

                                     aaaGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGa                                                    <  1:7380793/93‑1 (MQ=255)

                                      aaGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGGGGCGCGGGGTGAGGGCCCgggg                                                   >  2:7168194/1‑91 (MQ=255)

                                      aaGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGAt                                                   <  1:3980935/93‑1 (MQ=255)

                                        gCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTCAGGCCCCGGATGc                                                 >  2:3431249/1‑93 (MQ=255)

                                             ggCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAg                                            >  2:7696363/1‑93 (MQ=255)

                                             ggCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAg                                            >  1:2897049/1‑93 (MQ=255)

                                              gCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGt                                           <  2:4080784/93‑1 (MQ=255)

                                              gCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGt                                           <  1:1379052/93‑1 (MQ=255)

                                                tGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTc                                         <  2:6980949/93‑1 (MQ=255)

                                                 gACCGTAAGGGTCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCa                                        <  1:2832913/93‑1 (MQ=255)

                                                  aCCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCAt                                       <  1:2446854/93‑1 (MQ=255)

                                                    cGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTCAGGCCCCGGATGCCAAAGTTCATCg                                     >  1:7414987/1‑93 (MQ=255)

                                                     gTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGc                                    >  1:2107277/1‑93 (MQ=255)

                                                     gTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGc                                    >  1:46732/1‑93 (MQ=255)

                                                     gTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGc                                    >  1:4925683/1‑93 (MQ=255)

                                                     gTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGAGGTCGAGCGGCTGGCCAGGCGCGGGGTCAGGCCCCGGATGCCAAAGTTCATCGc                                    >  2:7566934/1‑93 (MQ=255)

                                                      tAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGcc                                   <  1:3056370/93‑1 (MQ=255)

                                                      tAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGcc                                   >  1:375892/1‑93 (MQ=255)

                                                       aaGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCa                                  <  2:677694/93‑1 (MQ=255)

                                                       aaGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCa                                  <  2:6698969/93‑1 (MQ=255)

                                                       aaGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCa                                  <  2:3095522/93‑1 (MQ=255)

                                                       aaGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCa                                  <  1:7190360/93‑1 (MQ=255)

                                                        aGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAg                                 <  1:4244719/93‑1 (MQ=255)

                                                        aGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAg                                 >  1:253410/1‑93 (MQ=255)

                                                        aGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAg                                 >  2:2192755/1‑93 (MQ=255)

                                                        aGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAg                                 <  2:2516014/93‑1 (MQ=255)

                                                         ggggCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAgg                                >  1:5775671/1‑93 (MQ=255)

                                                          ggtcGAAGGGGACGGGGATGCCGGCAATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGt                               <  2:5992413/90‑1 (MQ=255)

                                                           ggCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTc                              <  1:320051/93‑1 (MQ=255)

                                                            gCGAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCg                             >  2:4390201/1‑93 (MQ=255)

                                                              gAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGGCCAGGATGCCAAAGTTACTGCCCAGgtggtg                           >  1:1741246/1‑93 (MQ=255)

                                                              gAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGGCAAAGTTCATCGCCAGGTCGTg                           >  2:5431582/1‑93 (MQ=255)

                                                              gAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTg                           <  1:769037/93‑1 (MQ=255)

                                                              gAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTg                           >  2:4499751/1‑93 (MQ=255)

                                                              gAAGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGGTCAGGCCCCGGATGGCAAAGTTCATCGCCAGGTCGTg                           >  2:5768645/1‑93 (MQ=255)

                                                                aGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGc                         <  1:1683985/93‑1 (MQ=255)

                                                                aGGGGACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGGTAATCGCCAGGTCGTGGc                         >  1:79598/1‑93 (MQ=255)

                                                                 ggggACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCt                        >  1:5540719/1‑93 (MQ=255)

                                                                  gggACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTg                       <  2:1979831/93‑1 (MQ=255)

                                                                   ggACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGc                      <  1:1907179/93‑1 (MQ=255)

                                                                   ggACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGc                      <  1:5607114/93‑1 (MQ=255)

                                                                    gACGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGcc                     <  2:7112829/93‑1 (MQ=255)

                                                                      cGGGGATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCgg                   <  2:4176452/93‑1 (MQ=255)

                                                                          gATGCCGTCCATCAGGGCGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGgctgccgctgcc               >  2:4605056/1‑93 (MQ=255)

                                                                          gATGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGcc               >  2:6593105/1‑93 (MQ=255)

                                                                           ccgCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCg              <  2:4235671/91‑1 (MQ=255)

                                                                           aTGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCg              >  2:3842608/1‑93 (MQ=255)

                                                                           aTGCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCg              <  2:3725056/93‑1 (MQ=255)

                                                                             gCCGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCgtt            <  1:3676137/93‑1 (MQ=255)

                                                                              ccGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTAGCTGCCGGTGCCgttg           <  2:6053814/93‑1 (MQ=255)

                                                                               cGTCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCgttgt          <  2:4776486/93‑1 (MQ=255)

                                                                                 tCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGttt        <  1:1804068/93‑1 (MQ=255)

                                                                                 tCCATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGttt        >  2:5107898/1‑93 (MQ=255)

                                                                                  ccATCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCCGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGCGGCTGCCGGTGCCGTTGtttt       >  2:6233890/1‑93 (MQ=255)

                                                                                    atgaGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTcc     <  1:4106544/90‑1 (MQ=255)

                                                                                    aTCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTcc     >  1:6686287/1‑93 (MQ=255)

                                                                                    aTCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTcc     <  1:159006/93‑1 (MQ=255)

                                                                                     tCAGGGCGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCg    <  1:307830/93‑1 (MQ=255)

ATGGCATCCATGTTGCCGAGCGAAACGGGAGCCAGTGAAAGCTGCGGCTGACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGC  >  NC_000913/4513572‑4513751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`