BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,566	A→G	D121G (GAC→GGC)	uxuA →	mannonate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,566	0	A	G	100.0%	987.0 / NA	314	D121G (GAC→GGC)	uxuA	mannonate hydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (145/169); total (145/169)

Only 100 of 328 total aligned reads displayed.

ACCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGACGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACA  >  NC_000913/4549476‑4549655

aCCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCg                                                                                         >  2:3835179/1‑93 (MQ=255)

aCCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCg                                                                                         >  1:554483/1‑93 (MQ=255)

aCCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCg                                                                                         >  2:7355514/1‑93 (MQ=255)

aCCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCg                                                                                         >  1:7820719/1‑93 (MQ=255)

aCCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCg                                                                                         >  2:4148617/1‑93 (MQ=255)

      cgcAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCa                                                                                   <  2:3587165/93‑1 (MQ=255)

       gcAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCaa                                                                                  >  1:4805806/1‑93 (MQ=255)

       gcAGTGTGGCATTCGCACCGTGCGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCaa                                                                                  <  1:171731/93‑1 (MQ=255)

         aGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAg                                                                                >  2:2797316/1‑93 (MQ=255)

             tgGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGctct                                                                            <  2:6924980/93‑1 (MQ=255)

                cATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTgcg                                                                         <  2:4433049/93‑1 (MQ=255)

                 aTTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTgcgc                                                                        <  1:6768832/93‑1 (MQ=255)

                  ttCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCt                                                                       >  1:5246402/1‑93 (MQ=255)

                  ttCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCt                                                                       >  1:6963584/1‑93 (MQ=255)

                    cGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTc                                                                     >  1:3796471/1‑93 (MQ=255)

                    cGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTc                                                                     <  2:4835178/93‑1 (MQ=255)

                      cACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGa                                                                   <  2:4688610/93‑1 (MQ=255)

                      cACCGTGTGCTACAAATTCATGCCGGTTCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGa                                                                   <  2:6766647/93‑1 (MQ=255)

                       aCCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGAc                                                                  >  1:6945212/1‑93 (MQ=255)

                          gtgtGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAg                                                               <  1:4338930/93‑1 (MQ=255)

                           tgtgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGa                                                              <  2:5683464/93‑1 (MQ=255)

                           tgtgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGa                                                              >  1:4523651/1‑93 (MQ=255)

                           tgtgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGa                                                              >  1:2252965/1‑93 (MQ=255)

                           tgtgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCCACCGGa                                                              >  1:6099831/1‑93 (MQ=255)

                            gtgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGAt                                                             <  2:555952/93‑1 (MQ=255)

                            gtgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGAt                                                             <  2:3614964/93‑1 (MQ=255)

                            gggCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGAt                                                             <  2:5804113/91‑1 (MQ=255)

                             tgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATc                                                            >  2:5512693/1‑93 (MQ=255)

                             tgCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATc                                                            <  1:5703939/93‑1 (MQ=255)

                              gCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCg                                                           >  2:2470713/1‑93 (MQ=255)

                               cTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGa                                                          <  1:831987/93‑1 (MQ=255)

                                tACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGaa                                                         <  1:1110900/93‑1 (MQ=255)

                                tACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGaa                                                         >  2:2611747/1‑93 (MQ=255)

                                  cAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAAtt                                                       <  2:6097171/93‑1 (MQ=255)

                                   aaCTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAAttg                                                      >  2:6684877/1‑92 (MQ=255)

                                   aaCTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTc                                                      <  2:7475339/93‑1 (MQ=255)

                                     cTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGc                                                    <  1:6252785/93‑1 (MQ=255)

                                      ttCACGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCt                                                   <  2:3064415/93‑1 (MQ=255)

                                       tCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTg                                                  >  1:7506427/1‑93 (MQ=255)

                                          tGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCAt                                               >  1:318066/1‑93 (MQ=255)

                                          tGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCAt                                               <  1:4139184/93‑1 (MQ=255)

                                          tGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATAGAATTCGCTGCAt                                               >  1:5829200/1‑93 (MQ=255)

                                           gCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCAtt                                              >  1:5343258/1‑93 (MQ=255)

                                            ccGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTc                                             >  1:2553353/1‑93 (MQ=255)

                                              ggTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGa                                           >  2:7065638/1‑93 (MQ=255)

                                               gTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGaa                                          >  1:2925836/1‑93 (MQ=255)

                                                tGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGaaa                                         <  1:7396462/93‑1 (MQ=255)

                                                tGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGaaa                                         <  1:2671138/93‑1 (MQ=255)

                                                    cGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCa                                     >  2:1884114/1‑93 (MQ=255)

                                                    cGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCa                                     <  2:1719463/93‑1 (MQ=255)

                                                     gACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTTGCTGCATTCGAAATGCat                                    >  2:6256068/1‑93 (MQ=255)

                                                      aCTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCata                                   <  2:6595695/93‑1 (MQ=255)

                                                      aCTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCata                                   <  2:1579883/93‑1 (MQ=255)

                                                      aCTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCata                                   <  2:1108798/93‑1 (MQ=255)

                                                      aCTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCata                                   <  1:972806/93‑1 (MQ=255)

                                                      aCTGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCata                                   >  1:520657/1‑93 (MQ=255)

                                                      aCTGGACCAGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCata                                   <  1:3568886/93‑1 (MQ=255)

                                                        tGGACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATc                                 >  2:2862332/1‑93 (MQ=255)

                                                         ggACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATcc                                <  1:7772995/93‑1 (MQ=255)

                                                         ggACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATcc                                <  2:6981288/93‑1 (MQ=255)

                                                          gACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCt                               <  1:2003191/93‑1 (MQ=255)

                                                          gACCCGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCt                               >  1:1010602/1‑93 (MQ=255)

                                                            cccGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGa                             >  2:2245674/1‑93 (MQ=255)

                                                              cGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGaaa                           <  2:5218878/93‑1 (MQ=255)

                                                              cGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGaaa                           <  2:5059547/93‑1 (MQ=255)

                                                              cGTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGaaa                           <  2:776657/93‑1 (MQ=255)

                                                               gTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAAc                          >  2:3417612/1‑93 (MQ=255)

                                                               gTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAAc                          <  1:6686122/93‑1 (MQ=255)

                                                               gTACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAAc                          >  1:4041504/1‑93 (MQ=255)

                                                                tACTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACg                         >  1:6183444/1‑93 (MQ=255)

                                                                 aCTGCCCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGc                        <  1:4691934/93‑1 (MQ=255)

                                                                 aCTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGc                        >  1:7252932/1‑93 (MQ=255)

                                                                 aCTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGc                        <  1:2739310/93‑1 (MQ=255)

                                                                  cTGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGcc                       >  2:3965771/1‑93 (MQ=255)

                                                                   tGACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGccc                      >  1:2876385/1‑93 (MQ=255)

                                                                    gACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCa                     <  1:6487912/93‑1 (MQ=255)

                                                                    gACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCa                     <  1:4785439/93‑1 (MQ=255)

                                                                    gACCTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCa                     <  1:3710318/93‑1 (MQ=255)

                                                                       cTCGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGgc                  <  1:1081264/93‑1 (MQ=255)

                                                                         cGAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGgcgc                <  2:453175/93‑1 (MQ=255)

                                                                          gAATACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGcgcg               >  1:1787522/1‑93 (MQ=255)

                                                                           aaTACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCgg              <  2:7908602/93‑1 (MQ=255)

                                                                           aaTACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCgg              >  2:2307064/1‑93 (MQ=255)

                                                                           aaTACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCgg              >  2:6353169/1‑93 (MQ=255)

                                                                            aTACGTGCTGTCAGGCGGCCCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGa             <  1:1306590/93‑1 (MQ=255)

                                                                            aTACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGa             >  2:7227985/1‑93 (MQ=255)

                                                                            aTACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGa             <  1:3053563/93‑1 (MQ=255)

                                                                             tACGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGaa            >  1:3140068/1‑93 (MQ=255)

                                                                              aCGTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAg           <  1:2169234/93‑1 (MQ=255)

                                                                                gTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAGACGCCCAGGCGCGGAAGCg         <  2:7623406/93‑1 (MQ=255)

                                                                                gTGCTGCCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCg         >  2:1287715/1‑93 (MQ=255)

                                                                                    tgcCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGAtt     <  1:7170042/93‑1 (MQ=255)

                                                                                     gcCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCTGAAGCGGATTa    >  2:4112081/1‑93 (MQ=255)

                                                                                     gcCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTa    <  2:6734469/93‑1 (MQ=255)

                                                                                     gcCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTa    >  2:4956341/1‑93 (MQ=255)

                                                                                     gcCAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTa    <  1:1687397/93‑1 (MQ=255)

                                                                                       cAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTaca  <  1:5032149/93‑1 (MQ=255)

                                                                                       cAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTaca  <  1:1839780/93‑1 (MQ=255)

                                                                                       cAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTaca  >  2:2082819/1‑93 (MQ=255)

                                                                                       cAGGCGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTaca  <  2:801255/93‑1 (MQ=255)

ACCTGGCGCAGTGTGGCATTCGCACCGTGTGCTACAACTTCATGCCGGTGCTCGACTGGACCCGTACTGACCTCGAATACGTGCTGCCAGACGGCTCCAAAGCTCTGCGCTTCGACCAGATCGAATTCGCTGCATTCGAAATGCATATCCTGAAACGCCCAGGCGCGGAAGCGGATTACA  >  NC_000913/4549476‑4549655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`