BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,575,878	A→G	Y344H (TAT→CAT)	mcrB ←	McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,575,878	0	A	G	99.3%	960.9 / ‑3.8	303	Y344H (TAT→CAT)	mcrB	McrB
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (124/177); total (125/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 304 total aligned reads displayed.

CTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTT  >  NC_000913/4575788‑4575967

 tGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTc                                                                                        >  1:398468/1‑93 (MQ=255)

 tGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTc                                                                                        >  2:1351627/1‑93 (MQ=255)

    ttttttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTcaac                                                                                     >  1:5484725/1‑93 (MQ=255)

    ttttttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTcaac                                                                                     <  2:1562657/93‑1 (MQ=255)

    ttttttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTcaac                                                                                     >  2:6211514/1‑93 (MQ=255)

     tttttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTcaaca                                                                                    >  2:5383693/1‑93 (MQ=255)

     tttttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTcaaca                                                                                    >  2:2318219/1‑93 (MQ=255)

      ttttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTcaacaa                                                                                   <  1:7390540/93‑1 (MQ=255)

       tttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCaacaac                                                                                  <  1:1350255/93‑1 (MQ=255)

        ttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACg                                                                                 <  2:7754045/93‑1 (MQ=255)

        ttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACg                                                                                 >  2:4510193/1‑93 (MQ=255)

        ttATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACg                                                                                 <  1:7792383/93‑1 (MQ=255)

         tATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACCACgg                                                                                >  1:1252117/1‑93 (MQ=255)

         tATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACgg                                                                                >  2:4773166/1‑93 (MQ=255)

         tATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACgg                                                                                >  2:1662406/1‑93 (MQ=255)

          aTTCAGTAAAAAATGCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGc                                                                               <  1:4124007/93‑1 (MQ=255)

           ttCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGcc                                                                              >  1:2739148/1‑93 (MQ=255)

            tCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCa                                                                             <  2:4639687/93‑1 (MQ=255)

              aGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCaga                                                                           <  2:1515995/93‑1 (MQ=255)

              aGTAAAAAATTCAGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCaga                                                                           <  2:5160493/93‑1 (MQ=255)

               gTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCagag                                                                          <  2:3298733/93‑1 (MQ=255)

               gTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCagag                                                                          <  1:2041791/93‑1 (MQ=255)

                tAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCagaga                                                                         <  2:3835901/93‑1 (MQ=255)

                tAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCagaga                                                                         >  1:6985362/1‑93 (MQ=255)

                tAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCagaga                                                                         <  1:6777893/93‑1 (MQ=255)

                tAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCagaga                                                                         >  2:6965824/1‑93 (MQ=255)

                  aaaaaTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGc                                                                       <  2:3927689/93‑1 (MQ=255)

                   aaaaTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCg                                                                      >  2:7397382/1‑93 (MQ=255)

                   aaaaTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCg                                                                      >  2:5736039/1‑93 (MQ=255)

                    aaaTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGa                                                                     >  1:1756888/1‑93 (MQ=255)

                    aaaTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGa                                                                     <  2:4209500/93‑1 (MQ=255)

                     aaTTCCGGAGCGGGGGTGTATCAAAACCTGGCTCGATATCTATGAAAGAAAAGCGTCTGCGTAGGGCTTGGTCAACATCGGCCAGAGAGTGAt                                                                    <  1:3144549/93‑1 (MQ=255)

                     aaTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGAt                                                                    >  2:6486676/1‑93 (MQ=255)

                      atcccGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATc                                                                   <  2:1085849/90‑1 (MQ=255)

                      aTTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATc                                                                   >  2:2903156/1‑93 (MQ=255)

                       ttCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCg                                                                  <  1:842191/93‑1 (MQ=255)

                          cGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCa                                                               >  1:5721016/1‑93 (MQ=255)

                          cGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCa                                                               <  2:3478843/93‑1 (MQ=255)

                           ggAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAg                                                              <  2:5560291/93‑1 (MQ=255)

                           ggAACGGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAg                                                              <  1:6574057/93‑1 (MQ=255)

                             aaCTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTa                                                            <  2:3258754/93‑1 (MQ=255)

                              aCTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTAt                                                           <  2:1007019/93‑1 (MQ=255)

                               cTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTAtt                                                          <  2:4972338/93‑1 (MQ=255)

                               cTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTAtt                                                          >  1:1137782/1‑93 (MQ=255)

                                tgtgGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTc                                                         <  1:7654699/93‑1 (MQ=255)

                                tgtgGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTc                                                         >  2:1746223/1‑93 (MQ=255)

                                 gtggtgTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCa                                                        <  2:3753754/93‑1 (MQ=255)

                                 gtggtgTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCa                                                        <  2:7622041/93‑1 (MQ=255)

                                 gtggtgTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCa                                                        <  1:2173071/93‑1 (MQ=255)

                                  tggtgTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCAt                                                       <  2:277782/93‑1 (MQ=255)

                                   ggtgTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCAtt                                                      >  1:4650780/1‑93 (MQ=255)

                                    gtgtATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTa                                                     <  1:2962175/93‑1 (MQ=255)

                                     tgtATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTaa                                                    <  1:6371541/93‑1 (MQ=255)

                                     tgtATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTaa                                                    <  1:4851091/93‑1 (MQ=255)

                                       tATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAAc                                                  <  1:5541670/93‑1 (MQ=255)

                                         tCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCg                                                >  2:591134/1‑93 (MQ=255)

                                          cAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCga                                               <  2:6329402/93‑1 (MQ=255)

                                          cAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCga                                               <  1:3419666/93‑1 (MQ=255)

                                          cAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCga                                               <  2:3616105/93‑1 (MQ=255)

                                               ccTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGata                                          >  1:28222/1‑93 (MQ=255)

                                                cTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGatat                                         >  1:7487410/1‑93 (MQ=255)

                                                cTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGatat                                         >  2:7523933/1‑93 (MQ=255)

                                                cTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGatat                                         <  2:1975580/93‑1 (MQ=255)

                                                 tGGCTCAATATCTATGAAAGAAAATCGTCGGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGAtata                                        <  1:3889719/93‑1 (MQ=255)

                                                   gCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATaaa                                      >  1:5627053/1‑93 (MQ=255)

                                                    cTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAAc                                     <  2:5794256/93‑1 (MQ=255)

                                                     tCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACa                                    >  2:3138814/1‑93 (MQ=255)

                                                     tCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACa                                    >  2:7329392/1‑93 (MQ=255)

                                                      cAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAACCCGATGATATAAACAt                                   <  2:5217861/93‑1 (MQ=255)

                                                      cAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAt                                   <  1:6910926/93‑1 (MQ=255)

                                                      cAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAt                                   >  1:5133252/1‑93 (MQ=255)

                                                      cAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAt                                   >  2:711048/1‑93 (MQ=255)

                                                       aaTATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAtt                                  >  2:5996657/1‑93 (MQ=255)

                                                       aaTATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAtt                                  <  2:2425010/93‑1 (MQ=255)

                                                       aaTATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAtt                                  <  1:1526069/93‑1 (MQ=255)

                                                       aaTATCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACAtt                                  >  1:6474017/1‑93 (MQ=255)

                                                        atatCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATtc                                 <  2:6663515/93‑1 (MQ=255)

                                                        atatCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATtc                                 <  1:7262973/93‑1 (MQ=255)

                                                        atatCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATtc                                 >  2:4098396/1‑93 (MQ=255)

                                                          atCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATtctc                               <  1:3697737/93‑1 (MQ=255)

                                                           tCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTcc                              >  1:5897636/1‑93 (MQ=255)

                                                           tCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTcc                              >  1:2459608/1‑93 (MQ=255)

                                                           tCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTcc                              <  1:6109541/93‑1 (MQ=255)

                                                           tCTATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTcc                              >  2:373303/1‑93 (MQ=255)

                                                             tATGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCgg                            >  1:427980/1‑93 (MQ=255)

                                                              aTGAAAGAAAATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCggg                           >  2:1194887/1‑93 (MQ=255)

                                                                gaaagaaaATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGAc                         >  1:1436581/1‑93 (MQ=255)

                                                                 aaagaaaATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACa                        <  1:4398006/93‑1 (MQ=255)

                                                                   agaaaATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGCTATAAACATTCTCCGGGACATa                      <  2:4982466/93‑1 (MQ=255)

                                                                    gaaaATCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAg                     <  1:456009/93‑1 (MQ=255)

                                                                        aTCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATc                 >  2:1300726/1‑93 (MQ=255)

                                                                         tCGTCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCg                <  1:1933711/93‑1 (MQ=255)

                                                                            tCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGttc             >  1:7737997/1‑93 (MQ=255)

                                                                            tCTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGttc             >  1:3185547/1‑93 (MQ=255)

                                                                             cTGCGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGttct            <  2:2658387/93‑1 (MQ=255)

                                                                                cGTAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCa         <  1:755044/93‑1 (MQ=255)

                                                                                  tAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATc       <  2:294840/93‑1 (MQ=255)

                                                                                  tAGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATc       <  1:4699305/93‑1 (MQ=255)

                                                                                   aGGGCATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCg      >  1:6532054/1‑93 (MQ=255)

                                                                                       cATGGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGtttt  <  2:7922908/93‑1 (MQ=255)

CTGCTTTTTTATTCAGTAAAAAATTCCGGAACTGTGGTGTATCAAAACCTGGCTCAATATCTATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTT  >  NC_000913/4575788‑4575967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`